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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Rho associated kinase inhibitor (10μM, Y-27632) and Geltrex (0.5 μl/cm2 well surface area, Thermo Fisher Scientific, A1413302) were added to media during replating ...
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bioRxiv - Neuroscience 2022Quote: ... was in vitro phosphorylated overnight at 30°C by incubation with purified PLK3 kinase (4.2 μg/ml, #PV3812, ThermoFisher) in a buffer consisting of 20 mM Hepes pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining beads were resuspended in 20 μl of the radiolabelling mixture (10U of T4 polynucleotides Kinase (Thermo Scientific), reaction buffer A (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and rho kinase inhibitors such as a CEPT cocktail consisting of 5 µM Emricasan (50-136-5235, Fisher Scientific), 50 nM Chroman 1 (7163 ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then washed three times with PBS containing 1% BSA followed by 1 h incubation with donkey anti-mouse Alexa Fluor 488-conjugated secondary antibody (1:1000, ThermoFisher) at 4°C in the dark ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202; anti-rabbit-Alexa568, Invitrogen A10042 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30, 1:250, Invitrogen; Rabbit anti-MyosinVIIa ...
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bioRxiv - Cell Biology 2019Quote: ... The parasites were subjected to incubation with secondary antibody (1:400 anti-rabbit Alexa 568, 1:400 anti-rabbit Alexa 488, 1:250 anti-mouse Alexa 594 from Invitrogen) for 1.5 hours followed by three washes with 1X PBS for 10 minutes each ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000, goat α-rat (AF488, RRID:AB_2534074) at 1:2000, goat α-mouse (AF594, RRID:AB_141372) from Invitrogen (1:2000). Images were quantified using FIJI software (National Institutes of Health ...
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bioRxiv - Biochemistry 2022Quote: ... 1:100 dilution of mouse anti- alpha 1 Spectrin (ab11751) and 1:500 dilution of rabbit anti-KCNN4 (76647, Invitrogen). For co- immunostaining experiments with rabbit primary antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... fluorescent secondaries were added overnight at 4°C (goat-anti rabbit AlexaFluor 647 1:500, goat anti-mouse AlexaFluor 568 1:500, goat anti-guinea pig AlexaFluor 594 1:500; all ThermoFisher). Ovarioles were hand-dissected from whole fixed ovaries prior to imaging.
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin antibody complexes were isolated using protein A-beads for rabbit primary antibodies or G-beads for mouse primary antibodies (Dynabeads, Invitrogen). The PCRs with 1:20 dilutions of genomic DNA (input ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-TRF1 antibody (Goat Pab sc-1977, SantaCruz), or anti-PAR (Mouse Mab 10H ALX-804220, Alexis) recovered with Protein-G dynabeads (Invitrogen), run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins ...
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bioRxiv - Neuroscience 2020Quote: ... MS/MS database search was performed against in silico tryptic digest of mouse proteins UniProt FASTA database using SEQUEST HT within Proteome Discoverer 2.2 (PD 2.2, Thermo Fisher Scientific) with precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.02 Da ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316 ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
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bioRxiv - Systems Biology 2020Quote: ... Secondary detection of bait proteins was performed in 2.5% bovine serum albumin (BSA) in PBS using goat anti-mouse Alexa FluorTM 488 (Molecular Probes, ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The Mex67-3Flag tagged protein was isolated using 200 µL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 34 µg of anti-Flag M2 mouse monoclonal antibody (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... The autophosphorylation of hIRE1 KR was confirmed by western blot using a pSer/24 phospho-specific antibody (Genentech) and phosphorylated Pumilio proteins were detected using anti-mouse-V5 antibody (Invitrogen). In the case of kinase radioactive assays ...
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bioRxiv - Molecular Biology 2022Quote: ... The combined data set of 9 injections were mapped against the mouse protein database using Proteome Discoverer v2.4 and the Sequest HT search algorithm (Thermo Scientific) with label-free quantitation (LFQ ...
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bioRxiv - Neuroscience 2021Quote: NAc samples were pooled from four D1-Cre-RT or D2-Cre-RT male and female mice and RNA isolation from immunoprecipitated polyribosomes using primary mouse anti-HA antibody (Covance Research Products, Cat#MMS101R) and secondary antibody coated magnetic Dynabeads (Dynabeads protein G, Invitrogen) to pulldown the MSN specific RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP purification was carried out as previously described 48 with 2 mg of protein extract and 50 μl of Dynabeads Pan Mouse IgG (Invitrogen) per sample ...
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bioRxiv - Microbiology 2020Quote: ... Cleavage of junctional proteins was detected by Western blot analysis using specific antibodies against E-cadherin (Mouse, mAb, Thermo Scientific), p120-catenin (Rabbit ...
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bioRxiv - Immunology 2020Quote: ... slides were stained with the following secondary antibodies: polyclonal goat anti-Mouse Alexa Fluor488 to detect CD68 protein (Green color) (A-11029, Invitrogen) or polyclonal goat anti-Rabbit Alexa Fluor546 to detect SP140 protein (Orange color ...
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bioRxiv - Neuroscience 2022Quote: ... 50 and 35µg of proteins (for cell lysates, mouse brains and human cortices, respectively) were added with 1x Sample buffer (Thermo Fisher) and 1x Sample Reducing Agent (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2022Quote: We synthesized the Bowhead whale CDKN2CRTG protein with mouse codon usage (GeneScript) and directly cloned the synthesized gene into the pcDNA3.1-C-eGFP vector (Life Technologies), which added a C-terminal eGFP tag ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... proteins were extracted from homogenized mouse placental tissues or cell samples using RIPA lysis buffer (89900, Thermo Fisher Scientific, USA) containing protease and phosphatase inhibitors (A32961 ...
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bioRxiv - Cancer Biology 2022Quote: ... The horseradish peroxidase (HRP)-conjugated Protein A or HRP-conjugated rabbit anti-mouse secondary antibody for immunoblotting were from Invitrogen. The alpha smooth muscle actin (# ab7817) ...
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bioRxiv - Molecular Biology 2023Quote: The raw data files acquired from each sample were searched against mouse protein sequences database using the Proteome Discoverer 1.4 software (Thermo Scientific) based on the SEQUEST algorithm ...
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bioRxiv - Genetics 2022Quote: ... About 50ug total protein from cell extracts were blotted and hybridised with the primary antibody of Mouse anti-V5 (Invitrogen) for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: The raw spectra were searched against mouse protein database (UniProt) by SEQUEST using Proteome Discoverer (v2.5, Fisher Scientific, Waltham, MA). The mass tolerance was set as 20 ppm for precursors and 0.5 Da for fragments ...
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bioRxiv - Neuroscience 2023Quote: ... coated 10-cm dishes at a density of 100,000 cells/cm2 or on coverslips coated by 20 µg/ml poly-D-lysine and 10 µg/ml laminin mouse protein (Gibco) at a density of 40,000 cells/cm2 in plating medium (high glucose DMEM medium containing L-glutamine and 10% fetal bovine serum) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the membrane protein lysates were incubated with 10 μg mouse monoclonal anti-ubiquitin (eBioP4D1) antibody (Invitrogen, 14-6078-82) at 4 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD proteins were either precomplexed with StrepMAB-Classic HRP (IBA) and secondary goat anti-mouse IgG Alexa Fluor 555 (Invitrogen) in a 4:2:1 molar ratio or conjugated with pA-LS NP in a 1:1 molar ratio ...
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bioRxiv - Neuroscience 2021Quote: ... and goat anti-mouse Alexa Fluor 568 (1:500; Invitrogen A-11004). Expression was quantified in 3 hippocampal sections from 3 injected mice (n = 9) ...
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bioRxiv - Biochemistry 2020Quote: ... mouse Alexa Fluor 488 (Molecular Probes, Eugene, OR, US, 1/1000 dilution) and secondary rabbit (Dako ...
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bioRxiv - Genomics 2021Quote: ... or Fluor 568 conjugated donkey anti-mouse (1:100, Invitrogen, cat.no. A10037), in the dark for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-yeast PGK (22C5D8, Thermo Fisher Scientific, 4592250, 1:3000), goat anti mouse IgG-Alexa 680 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with 1:1000 α-mouse-AF488 (Molecular Probes A11001) in 2 % BSA [PBS] for 30 min at RT in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG (H+L) coupled to AlexaFluor594 (1/200; Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Cell Biology 2020Quote: ... or Alexa Fluor 594 goat anti-mouse (1 µg/ml, Invitrogen #A10239).
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-Clathrin Heavy Chain (1:500, Thermo Fisher, clone X22). Before fixation ...
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Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressorbioRxiv - Genetics 2021Quote: ... or a 1:10,000 dilution of mouse monoclonal antibody to Pgk1 (Invitrogen), followed by a 1:10,000 dilution of sheep anti-mouse antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2020Quote: HRP-conjugated goat anti–mouse (1:5,000; G-21040; Thermo Fisher Scientific)
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies used: goat anti-mouse Alexa Fluor 568 (1:500; Invitrogen), goat anti-rabbit 488 (1:500 ...