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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Dried residues were resuspended in MilliQ water and total protein content of each homogenate was measured with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). 20 µl of each sample was injected into a 25 cm x 4.6 mm C18 Nucleosil column with 5-µm particles (Interchim ...
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bioRxiv - Cancer Biology 2024Quote: ... cells scraped off and transferred to a 1.5 ml tube for cell lysis before clearance by centrifugation and protein concentration measurement using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) following manufactureŕs instructions and the FLUOstar Omega reader ...
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bioRxiv - Biophysics 2024Quote: ... HPG labeling of proteins was detected using the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit (Life Technologies, cat. #C10429), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and ventral hippocampus were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein content was measured in each well at the end of the assay using the Qubit Protein Assay Kit (Molecular Probes, Eugene, OR, USA). Oxygen consumption rate (OCR ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... The supernatant (islet sample) was collected and the total protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were subsequently re-solubilized in 40 uL of 5% SDS (v/v) and assayed for protein using the PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). 130-170ug of protein/sample (in a total volume of 33 uL) ...
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bioRxiv - Biochemistry 2022Quote: ... were centrifuged at 16,000 g for 30 minutes at 4 °C and the supernatant was collected for protein estimation using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific; Cat# 23227). Equal amounts of liver protein (100 μg ...
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bioRxiv - Cell Biology 2022Quote: ... The intensity values were normalized by the total protein in each sample (determined by the Pierce™ BCA Protein assay kit, ThermoFisher Scientific, cat# 23225) before they were used for quantitative analysis.
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bioRxiv - Plant Biology 2023Quote: ... The nuclear and cytosolic fractions were boiled in 1X Laemmli buffer (Laemmli, 1970) for 10mins at 100°C and protein was quantified using BCA compatible protein kit (Thermo Fisher Scientific, Whatham, USA). Fifty micrograms of protein were loaded onto a 4-20% gradient SDS-PAGE denaturing protein gel as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected and protein concentration was determined using the Pierce™ BCA protein assay kit following manufacturer’s instructions (Thermo Fisher Scientific, Massachusetts, USA). Each sample was separated by non-reducing SDS-PAGE with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and kept on ice and protein concentration measured with a Qubit™ protein BR assay kit (Thermo Fisher Scientific, Waltham, MA) and samples stored at − 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... A fraction of this neuronal mixture was taken to measure protein concentration through Pierce ™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, A53225) and the rest was used to isolate the biotinylated synaptic cleft proteins following the steps specified in Loh et al.24 In this sense ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% tri-tion-x-100) with 1% protein/phosphatase inhibitor (ThermoFisher; 78442). The RIPA lysate was sonicated and centrifuged for 15 min at 14,000 rpm at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and ∼1 µg primary antibody conjugated with protein-A dynabeads (Thermo Fisher) for EIF4A3-IP or CASC3-IP ...
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bioRxiv - Biochemistry 2022Quote: ... at 1:5000 dilution and MagicMark XP Western Protein Standard (Life Technologies).
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bioRxiv - Genetics 2020Quote: ... using a total of 1 μg of Cas9 protein (ThermoFisher Scientific, USA) per 0.5 ×105 cells.
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bioRxiv - Neuroscience 2020Quote: ... Rat anti-glial acidic fibrillary protein (GFAP; 1:1000, 13-0300, ThermoFisher), mouse anti-β-catenin (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... PAG1 (Csk-binding protein antibody PAG-C1) 1:1000 (ThermoFisher #MA1-19287); Phospho-Src family (Tyr416 ...
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bioRxiv - Cell Biology 2020Quote: ... and amyloid precursor protein (APP) at 1:500 (Invitrogen 14-9749-80). Imaging was performed with a BioRad ChemiDoc system and quantification was performed using ImageJ software.
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bioRxiv - Cell Biology 2020Quote: ... for 1 h with 40 μl of Dynabead-protein G (Life Technologies). Beads were washed with TSE-150 (0.1% SDS ...
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bioRxiv - Microbiology 2021Quote: ... Human alpha-1 PDX (alpha1-PDX) recombinant protein was purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5 μg of FGFb Recombinant Human Protein and 1% Gluta-MaxTM (Gibco) in flasks coated with Matrigel Matrix Basement Membrane (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 ng/mL Human TGF-β 1 recombinant protein (Cat# PHG9204, Gibco) and 1 μg/mL L-Ascorbic acid 2-phosphate (Cat# A8960 ...
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bioRxiv - Plant Biology 2024Quote: ... The protein pellet was resuspended with Novex Nupage LDS buffer 1× (Invitrogen), and proteins were then denatured at 70 °C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were then mixed with LDS loading buffer (Invitrogen NP0007; 1×) and reducing agent (Invitrogen NP0009 ...
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bioRxiv - Immunology 2022Quote: ... RNA-bound proteins were eluted by adding 1 μg RNaseA (Thermo Scientific) in 100 μl 100 mM Tris-HCl ...
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bioRxiv - Genomics 2022Quote: ... 1 µg/μL of TrueCut Cas9 protein V2 (ThermoFisher, Waltham, MA, USA), and 20% phenol red buffer (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg Invitrogen™ Protein G Dynabeads™ (Thermo Fisher Scientific, USA) was added to ten low protein binding microcentrifuge tubes ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-β-Amyloid Precursor Protein (CT695) (1:500, Invitrogen, 51-2700), mouse anti-β-Amyloid (6E10 ...
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bioRxiv - Cell Biology 2021Quote: Subcellular protein fractionation and isolation of chromatin fractions was performed using the Subcellular Protein Fractionation Kit for Cultured Cells kit from Thermo Fisher, following the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol from Alexa Fluor®647 Labeling Kit (Microscale Protein Labeling Kit, cat. no. A30009; ThermoFisher Scientific) and stored at −20°C ...
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bioRxiv - Cell Biology 2024Quote: Lentivirus concentration kit (Cat. NO. 41101ES50) was acquired from Yeason. BCA protein assay kit (Cat. NO. FD2001) was obtained from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 180 μg of mitochondrial proteins were subjected to a click reaction using a commercial kit (Click-iT Cell Reaction Buffer Kit; Thermo Fisher), with 40 μM Tetramethylrhodamine (TAMRA)-azide (Sigma) ...
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bioRxiv - Genetics 2024Quote: ... The PierceTM BCA protein assay kit (cat. # 23225) and the LiquiColor triglyceride test kit (cat. # 2100-225) were purchased from Thermo Fisher Scientific and Stanbio Laboratory ...
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with DENV-1 Mochizuki strain (in-house product) was coated onto a Nunc Maxisorp ELISA plate (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... WB 1:20 000) and Protein A HRP–conjugated were purchased from Invitrogen (WB 1: 20 000). The HALO-Trap Agarose beads and V5-conjugated beads were purchased from ChromoTek and Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... WT RBA1 (residues 1-363) and RBA1 mutant proteins were cloned into the pFastBac 1 vector (Invitrogen) with an N-terminal SUMO-6×His tag ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Molecular Biology 2022Quote: ... was preincubated with 1:1 mixture of washed Dynabeads Protein A and G (1002D and 1004D, Invitrogen) for more than 30 min at room temperature and washed twice with lysis buffer to remove unbound antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... and proteins were detected using 1:1 solution of Pierce™ ECL Western Blotting Substrate (Thermo Scientific). A complete list of all primary and secondary antibodies used in the study is provided in the key resources table.
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bioRxiv - Molecular Biology 2020Quote: ... 20 % PEG400) and 5’ end was phosphorylated by treating RNA with T4 Polynucleotide Kinase (1x PNK buffer (Thermo Scientific), 1mM ATP) ...
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bioRxiv - Biochemistry 2020Quote: ... in which case the phosphorylation of the oligos was performed by using the T4 Polynucleotide Kinase from Thermo Fisher, according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 pmol was 5’-end labeled with 20 μCi of Ψ-P32 ATP using Polynucleotide Kinase (Thermo Fisher) and subsequently purified using an Illustra MicroSpin G-50 column (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... at a concentration of ∼150 µM and then 2 mM of ATP and 0.42 μg of PLK3 kinase ( Invitrogen) per 500 μg of protein were added ...
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bioRxiv - Microbiology 2020Quote: ... beads were magnetized and resuspended in 100 μl PNK mix [10 units of T4 polynucleotide kinase (EK0032, ThermoFisher Scientific), 10 μCi γ-32P-ATP in PNK buffer] ...
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bioRxiv - Biochemistry 2020Quote: Poly-A RNA 27mer and 40mer were chemically synthesized (Biomers) and 32P-labelled with T4 polynucleotide kinase (Thermo Fisher) or ScriptCap m7G Capping System (CellScript) ...
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bioRxiv - Plant Biology 2022Quote: ... cold ATP was used in the kinase assays at 200 μM and detection was using ProQ diamond stain (Invitrogen).