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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Assay plates were created by spotting 4 μL/well (1.5 pmol/well) from the intermediate plate onto 384-Well Optical Bottom Plates (Nunc, Cat#152041, same lot in the screening) with the Biomek FXP ...
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bioRxiv - Neuroscience 2021Quote: ... coated glass coverslips in 12-well plates (for immunofluorescence and live-cell imaging) in DMEM (Gibco) supplemented with 10% Fetal Bovine Serum (Hyclone ...
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bioRxiv - Cell Biology 2022Quote: ... washed in fresh media and plated onto a 96-well glass bottomed plate (160376, Thermo Fisher). K562 cells were then briefly spun down by centrifugation at 100g for 5 minutes and imaged on a Zeiss LSM710 NLO Laser Scanning confocal microscope.
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bioRxiv - Bioengineering 2021Quote: ... 200uL of the cell suspension was plated on YP-SUC plates and spread throughout the plate using glass beads (Fisher Scientific, #11-312B 4mm). The plates were wrapped in sterile ...
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bioRxiv - Neuroscience 2023Quote: ... The original INNOTEST kit plate was replaced with a 96-well MicroWell™ MaxiSorp™ flat bottom plate coated with V5 antibody (Invitrogen P/N-0705; 1:10000) in carbonate buffer (NaHCO3 0.1 mM ...
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bioRxiv - Neuroscience 2021Quote: ... culture dishes were either custom-made 10-15mm diameter glass-bottom dishes [acid-washed coverslip (Fisher Scientific #12-541-B 22×22-1.5) affixed over a hole in a 35mm petri dish (Falcon #351008 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 × 104 293T-GRET/-PalmGRET cells were seeded per well into a glass-bottom 8-well chamber (#1.5, Nunc™ Lab-Tek™ II Chamber Slide™ System, Thermo Scientific) coated with poly-D-lysine (Mw 75,000-150,000 Da ...
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bioRxiv - Cell Biology 2021Quote: Stem cell-derived β-cells were dissociated from clusters and plated onto 35 mm glass-bottom dishes coated with 5% Matrigel (Fisher Scientific, 354277). Cells were washed twice with basal KRBH solution composed of (mM) ...
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bioRxiv - Cell Biology 2023Quote: ... U2OS cells expressing mCherry-Geminin seeded on a 35-mm glass-bottom dish (Matsunami, Osaka, Japan) were transfected with the GFP-SART1 vector using Lipofectamine 2000 (Thermo Fisher Scientific, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed with PBST three more times before being placed in individual 2ul drops of PBS in a Nunc glass bottom dish (Thermo Fisher Scientific, 150682) and overlaid with mineral oil for imaging.
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bioRxiv - Cell Biology 2024Quote: ... cells were grown on glass bottom dishes in complete medium and stained for 30 min with 250 nM TMRM (Thermo fisher Scientific, T668) or 5 min with 100nM Mitotracker Deep Red (Thermo fisher scientific ...
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bioRxiv - Biochemistry 2024Quote: ... and placed on a Lloyd & McCown Woody Plant Basal Medium with Vitamins (PhytoTec Labs) agar pad sitting atop a 35 mm glass bottom dish (Thermo Fisher Scientific, 150682). The pad and seeds were incubated at 4 °C for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... and 0.2 ml were placed in duplicate in a black 96 well flat bottom plates (Nunc or Grenier Fluorotrac). Fluorescence was measured with a Tecan infinite 200 Pro machine ...
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bioRxiv - Pathology 2021Quote: ... Reactions were performed in triplicate in black 96-well plates with a clear bottom (Nunc, Thermo Fisher, Paisley, UK) with 85 μL of the reaction mix loaded into each well together with 15 μL of 0.1 mg/ml TBS-soluble or detergent-soluble fractions ...
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bioRxiv - Pathology 2021Quote: ... Reactions were performed in triplicate in black 96-well plates with a clear bottom (Nunc, Thermo Fisher, Paisley, UK) with 85 μL of the reaction mix loaded into each well together with 15 μL of 0.1 mg/ml TBS-soluble or detergent-soluble fractions ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μl from each sample was transferred to a 96-well flat bottom plate together with 100ul of Drabkin’s reagent (MilliporeSigma) containing Brij-35 solution (Thermofisher). The plate was incubated at room temperature for 15 min on a plate shaker ...
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bioRxiv - Biochemistry 2021Quote: ... 2000 parasites suspended in complete RPMI were added to the wells of optical bottom black 96 well plates (ThermoFisher) containing a confluent layer of HFF cells ...
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bioRxiv - Microbiology 2020Quote: ... 2500 Vero E6 (12 μl/well) or 10000 Calu-3 (12μl/well) were seeded in 384-well white optical-bottom tissue culture plates (Nunc) with the Multidrop Combi liquid handling instrument (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and 100 μl of each cell suspensions were loaded per well in round-bottom 96-well plates (Fisher Scientific). The samples were assayed in a Guava EasyCyte 6-2L HT Flow Cytometer (Luminex) ...
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bioRxiv - Bioengineering 2022Quote: ... FRET sensor-expressing HEK 293T cell line was seed at a density of 10,000 cells per well in a black clear bottom plate (Grener) with DMEM culture (11885, Gibco) supplemented with 10% FBS (Gibco 10082147) ...
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bioRxiv - Microbiology 2021Quote: ... while isolates intended for robot performed susceptibility testing were subcultured in a flat-bottom 96-well plate (Nunc, 167008), each well filled with 220 μL (allowing for overnight evaporation of roughly 20 μL ...
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bioRxiv - Microbiology 2021Quote: ... Each clone was inoculated into four wells in a flat-bottom 96-well plate (Thermo Scientific Cat. No. 167008) containing 7% LB-glycerol supplemented with 25 μg/ml chloramphenicol and 10 μg/ml streptomycin and incubated overnight at 37°C after which stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: All samples were prepared by transferring 2x105 cells per sample to an uncoated V-bottom 96-well plate (Nunc). The samples were spun down at 300 g for 5 min at room temperature and washed with 0.2 mL 1X PBS twice ...
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bioRxiv - Microbiology 2021Quote: ... The remaining co-cultures were diluted 1:10 to OD600nm 0.01 and pipetted into a round bottom 96-well plate (Nunc). Growth measurements were performed as described above for 72 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Six overnight cultures were diluted 1:1,000 into fresh LB in flat-bottom 96-well plates (Thermo Scientific, Denmark), which were incubated during 24 hours at 37 ºC 250 rpm ...
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bioRxiv - Immunology 2020Quote: ... and resulting single cell suspensions were cultured in the above media in flat bottom 48-well Nunclon plates (ThermoFisher), at 1× 106 cells/well ...
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bioRxiv - Pathology 2020Quote: ... by mixing 100 μL of lysate with 100 μL of D-luciferin (0,1 mg/mL) supplemented with ATP (0,5 M) in flat bottom 96-well black plates (Thermo Scientific). Luciferase activity was measured for 1 min with f/stop = 1 and binning = 8 ...
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bioRxiv - Biochemistry 2021Quote: ... and complemented with 7.5 μM of Site2hp RNA (4:1 protein:RNA molar ratio) in PBS buffer in a 384 black-well clear-bottom plate (Nunc). A ThT stock was prepared as described above and added to the well to 300 μM final concentration ...
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bioRxiv - Immunology 2020Quote: ... Cytokine concentrations were measured by TM ELISAs according to the manufacturer’s instructions in Nunc MaxiSorp flat-bottom plates (Invitrogen) using Uncoated ELISA Kits (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... in quadruplet were prepared in 96-well round-bottom plates using Dulbecco’s Modified Eagle Medium (DMEM) with 1% (v/v) FBS and 1% Antibiotic-Antimycotic (Gibco). 75 µL of the diluted sera was mixed with an equal volume of media containing 64 PFU of SARS-CoV-2 virus (hCoV-19/England/02/2020 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded at densities of 3 × 104 or 5 × 104 per 60 mm plate with a bottom layer of 0.6% Noble agar in MEM (Gibco) and a top layer of 0.5% methylcellulose containing MEGM (Lonza) ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... and liquid agarose (2.5mL) was used to cover the bottom of a high-performance #1.5 6-well plate (Fisher Scientific). This process was repeated for all five wells ...
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bioRxiv - Microbiology 2023Quote: ... The mixtures (180 μL) were assembled directly in Nunc 96-well microwell plate (round, black bottom, untreated, Thermofisher Scientific), mixed ...
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bioRxiv - Cancer Biology 2023Quote: Antitumor assays were carried out in triplicates in flat white 96-well plates with a clear bottom (Thermo Scientific). Per well ...
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bioRxiv - Physiology 2022Quote: In vitro bioluminescence was performed by seeding a range of cell densities (from 625 to 2 x104 cells/well) into an optical bottom 96-well plate with black walls (#165,305, ThermoFisher). The cells were allowed to attach for 3 h prior to the addition of 5.12 mM D-Luciferin ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at 3×106 cell density per well into Nunclon Sphera 96 U bottom plates (#174929; ThermoFisher Scientific) and incubated at 37 °C with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... we transferred 3 hCOs to each well of a white 96-well plate with transparent bottom (Nunc, ThermoFisher 165306) and performed media changes on samples for synchronization ...
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bioRxiv - Neuroscience 2024Quote: ... we transferred 3 hCOs to each well of a white 96-well plate with transparent bottom (Nunc, ThermoFisher 165306) and performed media changes on samples for synchronization ...
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bioRxiv - Immunology 2024Quote: ... Staining was performed for 30 min at room temperature in 96-well V bottom plates (Thermo-Fisher Scientific, USA) with the following antibodies against CD3 (clone ...
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bioRxiv - Immunology 2024Quote: ... MoDCs were removed from 12-well Nunclon™ Delta surface-treated flat bottom plates (Thermo Fisher Scientific, Waltham, MA), pelleted ...
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bioRxiv - Immunology 2024Quote: ... the cells were seeded at 5 × 104 cells per well in U-bottom 96-well plates (Fisher Scientific; #163320). Two dilutions of antibody ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 20 g/L peptone) in a 96-well V-bottom plate covered by breathable rayon sealing film (Nunc #241205) in a 30°C shaking incubator set to 300 rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... From these pre-cultures we used 5 μl to inoculate 384-well plates (Sigma-Aldrich, Nunc®, flat-bottom) containing 55 ul of SM media per well with inducer concentrations of 0.2% ...
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bioRxiv - Synthetic Biology 2022Quote: ... subsequently divided the culture into 48 well (48 replicate) of 96-Well Optical-Bottom Plate with Polymer Base (ThermoFisher) and treated them with Amp (0.1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was determined by seeding EwS cell lines at 0.25·105 cells/mL in clear bottom black walled 96-well Tissue Culture plates (Thermofisher) and incubated with 10 µL of Cell Titer Blue (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...