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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... round-bottom plate in neural induction medium (N2:Neurobasal, 1:1, Thermo Fisher Scientific, Waltham, Massachusetts) containing 2% B27 without vitamin A (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Growth assays were performed in Nunclon 96 Flat Bottom Transparent Polystyrene well plates (Thermo Fisher Scientific) containing 250 µl of M9 minimal medium supplemented with specified carbon sources (2 g/l unless stated otherwise ...
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bioRxiv - Bioengineering 2024Quote: ... Serum dilutions were prepared during the blocking step in a conical bottom plate (Thermo Scientific, 249570) in diluent buffer starting at 1:100 (1 μL serum into 99 μL diluent buffer ...
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bioRxiv - Bioengineering 2024Quote: ... beads were resuspended by pipetting and transferred to V-bottom 96-well microtiter plate (Thermo Scientific). Flow cytometric analysis was performed using MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2024Quote: ... The cell suspension was transferred to a 96-well optical bottom black-wall plate (Thermo Scientific) and the fluorescence was determined in a SpectraMax Gemini EM plate reader (Molecular Devices ...
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bioRxiv - Immunology 2024Quote: ... and cultured (1x106 cells/mL) in flat-bottom 48-well plates with RPMI1640 (#21875034 Gibco Thermofisher) supplemented with L-glutamine (#25030024 Gibco Thermofisher) ...
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bioRxiv - Neuroscience 2024Quote: Inner wells of a clear-bottom black 96-well plate (Greiner, 655090 or Thermo Scientific, 165305) were coated with 50 µL of 50 µg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... Fifty microliters were decanted in Nunclon Sphera 96U-well round bottom plate wells (Thermo Scientific 174929), the plate was centrifuged at 100g for 2 minutes ...
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bioRxiv - Biophysics 2021Quote: ... cells were transferred to glass-bottomed plates (Nunc Lab-Tek, 0.4 mL working volume) at ~25% confluence and allowed to recover for 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then transferred to 8-well imaging plates (No. 1.5 glass. ThermoFisher Scientific) and incubated at 37°C in 250 μls of 1 μM FLIPPER-TR probe (Spirochrome ...
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bioRxiv - Biophysics 2020Quote: ... the GMPCPP seeds were immobilized on the bottom of the chamber (= PEG-coated glass) via streptavidin (S888, Thermo Fisher Scientific), and 20 μL of WT or Y222F tubulin in BRB80 buffer solution containing 1 mM GTP was infused into the chamber ...
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bioRxiv - Cell Biology 2020Quote: ... A hole with a diameter of 17mm was drilled in the center bottom of a 60×15mm tissue culture dish (Falcon) and a glass slide (#2, 22×22mm, Mentzel, Thermofisher) was glued onto the bottom of the hole with aquarium sealant.
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bioRxiv - Cell Biology 2020Quote: ... Individual egg chambers from well fattened progeny of the right genotype were dissected and transferred to borosilicate glass bottom chambered coverglasses (ThermoFisher) for imaging ...
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bioRxiv - Biophysics 2020Quote: The GV_AC28 cells incubated with Dox for over 3 days were plated onto a 35 mm glass bottom dish filled with phenol red-free DMEM (Gibco) with supplements ...
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bioRxiv - Cell Biology 2022Quote: ... cells plated on glass bottom cell culture dish were loaded with 10 μmol/L Fura-2 acetoxymethyl ester (Molecular Probes) and 0.1% pluronic F-127 (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... cells grown in glass-bottom dishes in FBS-containing medium were stained with 5 μM laurdan (Molecular Probes, Eugene, OR) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on a glass bottom dish (Matsunami, Osaka, Japan) and in FluoroBrite DMEM medium (Thermo Fisher, Waltham, MA) to collect live-cell images ...
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bioRxiv - Microbiology 2020Quote: An initial inoculum of 104 cells/mL in Sabouraud medium was added to 35 mm glass bottom dishes (Thermo Scientific ™ Nunc Glass Bottom Dish ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293A cells expressing dopamine receptor sensors or other constructs were prepared on cover-glass-bottom dishes coated with 10 μg/ml of fibronectin (Gibco). Various concentration of dopamine or other ligands were added to the cells ...
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bioRxiv - Cancer Biology 2021Quote: For live imaging, cells in glass bottom dish (NEST, 801001) were transfected with pEGFP-Tubulin and mCherry-H2B using Lipofectamine 3000 (Invitrogen), and synchronized with double thymidine block procedure ...
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bioRxiv - Cell Biology 2023Quote: Cells were plated on glass-bottom dishes and were stained with 250 nM of MitoTracker™ Orange CMTMRos (Invitrogen, M7510) for 30 min then fixed in 4% paraformaldehyde (Alfa Aesar ...
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bioRxiv - Neuroscience 2022Quote: Primary spinal microglia grown on poly D-lysine coated glass-bottom dishes were loaded with Fura-2 by incubating cells in 2 uM Fura-2-AM (Invitrogen) in growth medium for 30 min at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: ... cortices were dissected and neurons were dissociated with trypsin and plated on poly-d-lysine–coated glass-bottom culture dishes in neurobasal media supplemented with B27 (Invitrogen). This same media was used for all time-lapse experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Heterozygous HeLa TFG-mClover-FLAG cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging with live imaging solution (Invitrogen A1429IDJ) supplemented with 10 mM final concentration of glucose (Sigma G8644-100ML) ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded in 35-mm glass-bottom dishes and were transfected with Treacle-FusionRed-Cry2 plasmid using LTX transfection reagent (Invitrogen) 24 h before imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... animals with beating hearts were selected for fluorescence for expressing the Mesp>NTR::tagRFP construct and placed individually inside a glass bottom Lab-Tek II Chambered #1.5 German coverglass system (Fisher Scientific) containing MTZ in FASWT ...
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bioRxiv - Neuroscience 2024Quote: Mouse DRG neurons were seeded on a glass-bottom cell culture dish and cultured in neurobasal medium (Gibco, Cat. 21103049) supplemented with B-27 supplement (Gibco ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... MGEs were dissected out from the organotypic slices and were embedded in matrigel matrix on glass bottom culture dishes (Nunc). MGE explants from Rac1/Rac3 wt and Rac1/Rac3 dmut were cultured in Neurobasal/B27 medium for 1 DIV in a 5% CO2 humidified incubator at 37 °C and next prepared for live imaging acquisition ...
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bioRxiv - Cell Biology 2022Quote: ... Individual egg chambers from well fattened progeny of the right genotype were dissected and transferred to borosilicate glass bottom chambered coverglasses (ThermoFisher) for imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... The testes were dissected into 1X Becker Ringer’ s solution [39] and then mounted onto a 35mm Glass Bottom Dishes (Nunc). 200 μL of 1 mg/mL poly-L-lysine (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... the 35 mm glass bottom dish was pre-incubated for 30 min with 1 ml of media containing Hoechst 33342 (1:1000 ThermoFisher) to label the nuclei ...
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bioRxiv - Developmental Biology 2024Quote: ... Denuded oocytes were washed in M2 media containing BSA and transferred to glass-bottom petri dishes (Thermo Scientific, cat#150680) containing a drop of M2 media containing BSA ...
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bioRxiv - Immunology 2020Quote: ... The positive control was diluted in 1.5 mM of sodium carbonate buffer pH 9.6 to 20 µg/ml and used for plate coating by adding 100 µL per well to a Nunc MaxiSorp(tm) flat-bottom plate (Thermo Fisher, Waltham, Massachusetts, USA) and incubating overnight at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... From the preculture plate we used 5 μl to inoculate each of four different wells of a 384-well plate (Sigma-Aldrich, Nunc®, flat-bottom) each containing 55 μl of SM containing:
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bioRxiv - Cancer Biology 2021Quote: PDOs were harvested as described above and incubated in 96-well round bottom culture plates (Thermo Fisher) in 100 µl ‘Type 1’ organoid culture medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed with PBS and the bottoms of the wells were visualized using EVOS (Invitrogen) or Lionheart (Biotek Instruments ...
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bioRxiv - Bioengineering 2020Quote: ... we fabricated self-organized spheroids using hMSCs on the Nunclon Sphera 96-well round-bottom plate (ThermoFisher). 2,500 cells were subcultured on the round-bottom plate to fabricate the spheroids and incubated for 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded in 4 flat-bottom 96-well plates (353072, Corning B.V. [Fisher Scientific, Madrid, Spain]) at a density of 1,000 cells/well in 200 μL of tetracycline-free media ...
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bioRxiv - Microbiology 2021Quote: The medium throughput screen was set up in sterile 96-well flat bottom polystyrene plates (Nunc: 260860). For each assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were then washed with PBS and the bottoms of the wells were visualized using EVOS (Invitrogen) or Lionheart (Biotek Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... was used to coat the bottom of each well of 4-well chamber plates (Lab-Tek, Nunc). The 4-well chamber plate was placed in 5% CO2 at 37°C for 30 minutes to allow the Matrigel to solidify ...
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bioRxiv - Biophysics 2022Quote: ... Turbidity of phase-separated samples was then estimated using 96-well NUNC optical bottom plates (Thermo Scientific) on a Multiskan Go (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: Single CD8+ YFV-specific T cells were index sorted into a 96-well V-bottom plate (Thermofisher) containing 9μl Tris-EDTA (TE ...
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bioRxiv - Cancer Biology 2022Quote: ... MS-5 cells were seeded into 0.2% gelatin coated 96 well-flat bottom plates (Thermo Fisher, 167008) at a density of 4-5×105 cells per well in Myelocult medium (H5100 ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions were subsequently mixed and added into 96-well conical-bottom microwell plates (Thermo Fisher Scientific) and allowed to settle overnight at room temperature prior to imaging.
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bioRxiv - Microbiology 2022Quote: ... and 0.2 ml were placed in duplicate in a black 96 well flat bottom plates (Nunc Fluorotrac). Fluorescence was measured with a Tecan infinite 200 Pro reader ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The 100-µl overnight cultures were dispensed into 96-well optical bottom black microtiter plates (Thermo Scientific). OD600 and fluorescence (excitation at 485 nm and emission at 535 nm with dichroic mirror of 510 nm ...
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bioRxiv - Synthetic Biology 2020Quote: ... in 96-well plates (flat bottom Nunclon™ Delta Surface, Thermo Fisher Scientific, 200 μL per well). Cell-specific mCherry fluorescence was determined by dividing the red fluorescence signal (excitation at λEx = 587 nm ...
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bioRxiv - Microbiology 2020Quote: ... 9×103 iPSCs are seeded into low-adhesion V-bottom 96-multiwell plates (277143; Thermo Fisher Scientific). The following day change medium with KSR medium (DMEM-F12 with 15% KSR ...
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bioRxiv - Immunology 2020Quote: ... and 50 μL of each supernatant were subsequently transferred to a flat-bottom microtiter well plate (Nunc). Hemolysis was then determined from the A405 measured on a Victor3 plate reader (PerkinElmer) ...