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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 μl of diluted DharmaFect #4 was pipetted in the three empty 96-well V-bottom plates (Thermo Fisher, cat. no. 11816003) and 5 μl of the 800 nM coupled gRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the sporozoites and the resulting mixtures transferred into the wells of an optical bottom 96-well plate (Thermo Fisher Scientific). The plate was centrifuged for 3 min at 1,000 rpm (Multifuge S1-R ...
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bioRxiv - Microbiology 2022Quote: ... of liquid culture of each investigated concentration was added in triplicate to a black Nunc MicroWell 96-well optical-bottom plate with polymer base (ThermoFisher Scientific, US). Absorbance (OD600 ...
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bioRxiv - Cancer Biology 2023Quote: 4T1 cells were plated at 2×104 cells per well in 200 uL of 7-day post-RT 3T3 CM supplemented with either 50 nM AZD3965 or an equivalent volume of DMSO (control) in a Nunclon Sphera ultra-low attachment 96-well U-bottom plate (Thermo Scientific 174929), allowing the cells to form spheroids ...
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bioRxiv - Microbiology 2024Quote: The sera were prediluted with phosphate buffered saline (PBS) to 1:100 in a polystyrene microtiter plate (Nunc F96 MicroWell flat bottom) and mixed well by shaking ...
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bioRxiv - Microbiology 2023Quote: ... Dd2-Polδ mixed-stage parasite cultures at 5% parasitaemia and 4% hematocrit were dispensed into each well of a six-well flat-bottom plate (Thermo Scientific, Australia). Five cultures were treated with 10x the growth EC50 of OGHL250 (9.1 µM ...
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bioRxiv - Cancer Biology 2023Quote: HCT116 cells were seeded at density of 400 cells/well on Nunclon Sphera 96-well U-bottom ultra low attachment 3D cell culture plate (Thermo Fisher Scientific) and spun down at 200 g for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: Pseudotyped viruses were titrated by serially diluting filtered pseudotypes with fresh DMEM starting from a 1:2 to a final 1:512 dilution using white 96-well flat bottom plates (ThermoFisher, Woolwich, UK). Then appropriate target cells were added (Table 1 ...
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bioRxiv - Immunology 2023Quote: ELISA to detect specific anti-NP IgG1 antibodies was performed in 96-well flat bottom Nunc-ImmunoTM MaxiSorpTM plates (Thermo Fisher Scientific). Plates were incubated ON using 10 μg/mL of NP(2,5)-BSA or NP(25)-BSA as the coating reagent kindly provided by Dr ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.2 mg µL-1 BCA standard were mixed with the collected proteins in 100 µL 1× RIPA buffer in a 96-well plate (Matrix Microplate w/lids 96-well blk/clr, flat bottom, tissue culture, PS; Thermo Fisher Scientific). Proteins were incubated at 37°C for 60 min and measured at a wavelength of 562 nm using a Synergy HTX Microplate Reader.
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... each culture was diluted 1:1,000 into fresh LB and was used to fill flat- bottom 96-well plates with 200 µL per well (Thermo Fisher Scientific). Eight replicates from each genotype were included ...
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bioRxiv - Microbiology 2023Quote: 1.68 x 105 ISE6 cells per well were seeded in a 96-well plate with black walls and clear bottoms (Thermo Scientific, 165305). All wells were treated with the fluorescent detection probes 2’,7’-dichlorofluorescein diacetate (10µM ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB-231 and C4-2B spheroids were formed by placing 20,000 cells in Nunclon Sphera round-bottom 96-well plates (Thermo Fisher Scientific, #174925) and centrifuging at 290xG for 3 minutes to form a pellet (day 0) ...
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bioRxiv - Cancer Biology 2023Quote: ... were seeded either alone or with TNBC-derived CAFs at a 1:1 ratio on low adherence 96-well round bottom plates (Thermo Fisher # 174925), in DMEM+10%FBS media ...
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bioRxiv - Biochemistry 2022Quote: ... 1×105 cells resuspended in PBS-B were added per well of a 96-well plate (V-bottom, non-treated surface; Thermo Fisher Scientific) and kept on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 3,000 MCF-7 cells and 10,000 HCC1500 cells were seeded in black 96-well optical bottom plates (Thermo Scientific™, CAT: 165305) in 100 µL per well ...
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bioRxiv - Immunology 2023Quote: ... of the CD3/CD15-depleted G-CSF-mobilized PBMCs were subsequently transferred to 96U-bottom ultralow adherence plates (Nunclon Sphera, Cat. 174925) to be cultured in StemPro34 serum-free medium (Gibco, Cat. 10639011) with 1x penicillin/streptomycin (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Target cells (1.5×104 in 25 µL per well) of each variant were seeded in serum free RPMI into a 96-well V-bottom plate (249952; Thermo Fisher Scientific). Heat inactivated mouse sera and BAL fluid samples were diluted 1:2 in serum free RPMI or used neat respectively ...
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bioRxiv - Microbiology 2023Quote: ... All compounds were dissolved in DMSO and 100 nL was transferred to a 384-well clear bottom plate (ThermoFisher Scientific, cat# 265203) using a Beckman Echo 650 acoustic liquid handler ...
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Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeastsbioRxiv - Systems Biology 2023Quote: ... an appropriate amount of inoculum and fresh media were transferred into 96-well clear U-bottom polypropylene microtiter plates (Thermo Fisher Scientific) in 4 replicates ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell suspensions were seeded in 50 μL of complete culture media and plated onto 384-well white clear-bottom plates (ThermoFisher Cat# 164610) using a MultidropTM Combi liquid dispenser in the slowest setting in triplicate ...
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bioRxiv - Cancer Biology 2023Quote: BLM spheroids were produced by seeding 104 cells per well in a 96-well U-bottom plate (Thermofisher Scientific, Waltham, MA, US) for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Triplicates of 200 uL for each strain cell culture were transferred into Nunc 96-well black/clear bottom plates (Thermo Scientific, 165305). mNG detection was performed using Ex ...
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bioRxiv - Immunology 2023Quote: ... the measurement of Fluorescence and the absorbance spectra was carried out using 1uM cSC in PBS in 100ul volume in a flat clear bottom 96 well plate (Thermo Scientific: 165305), and the absorbance and fluorescence spectra were measured using the Cytation 5 plate reader from BioTek ...
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bioRxiv - Immunology 2023Quote: ... a total of 75 × 103 labeled Teff cells were seeded in 96-well round-bottom plate in each well supplemented with Dynabeads™ Mouse T-Activator CD3/CD28 (Gibco, #11456D) at ration 1:1 beads to Teff cells ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 µL of subculture was added to 50 µL of 1X phosphate buffered saline (PBS) with 10 µg/mL of kanamycin in a 96-well optical bottom black plate (Thermo Scientific, #265301). Bulk optical density (OD600 ...
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bioRxiv - Biochemistry 2024Quote: ... The desthio-biotinylated mCNPase and hCNPase were immobilised for 2 h at RT on streptavidin-coated flat-bottom 96-well plates (Thermo Fisher Scientific). The wells were washed three times 10 min with PBS and blocked using 5% skim milk in PBS-T for 3 h at RT ...
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bioRxiv - Bioengineering 2024Quote: Human monocyte-derived macrophages (hMDM) were cultured in Nunc™ MicroWell™ 96-well optical-bottom plates (Thermo Fisher Scientific, Waltham, MA) and left untreated or treated with vehicle (sterile 1X PBS) ...
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bioRxiv - Microbiology 2024Quote: ... 10 µL of each of the overnight cultures was transferred to a separate well of a black 96- well plate with optical bottom (ThermoFisher Scientific, USA) containing 290 µL of SC medium ...
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bioRxiv - Microbiology 2024Quote: ... 10 µL of each of the overnight cultures was transferred to a separate well of a white 96-well plate with optical bottom (ThermoFisher Scientific, USA) containing 290 µL of SC medium and 1mM luciferin ...
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bioRxiv - Cell Biology 2024Quote: ... Hepatocytes (50,000 cells/well) were plated in a collagen I-coated clear-bottom 96 well plate (Thermo Fisher Scientific, 08-774-307). Porcine liver extracellular matrix (LECM ...
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bioRxiv - Microbiology 2024Quote: ... diluted 1,000-fold and 100 µl of each suspension were transferred in triplicates in a 96-well Nunc-plate with a flat bottom (Thermo Fisher Scientific). The growth was recorded as OD600 in Infinite F200 Pro plate reader (Tecan ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and plated at a density of 5×104 cells per well in a flat bottom 96-well plate with anti-CD3 anti-CD28 Dynabeads (Dynabeads™ Human T-Expander CD3/CD28 Cat. 11141D, Gibco,FR) and IL-2 (Proleukin ...
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bioRxiv - Immunology 2024Quote: ... The cells were cultured at a concentration of 2 million cells/mL in 96 well round (U) bottom plates (Thermo Fisher Scientific). Culture medium was RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... One follicle per well was cultured in round-bottom 96-well plates containing 100 µL of MEMα media with 100 U/mL penicillin/streptomycin (Gibco, Cat# 1520096), ITS-G (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... approximately 1-5×107 CFU of recombinant bacteria harbouring the plasmids of interest were dispensed into standard flat-bottom 96 well-plates (Thermo-Fisher Scientific; #167008).
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T-cells (105 cells/well) were seeded in supplemented RPMI-1640 medium into Nunclon delta surface 96-well U-bottom plates (Thermo Fisher Scientific)8,10,38 ...
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bioRxiv - Immunology 2024Quote: ... 1% L-Glutamine and plated at a density of 2-3 × 106 cells per well in a round U-bottom Nunclon Delta treated 96-well plates with lids (Thermo Fisher Scientific). Plate was then centrifuged at 500 x g for 5 min at 4 °C before being stimulated with 100 μl of complete media + 1X eBioscience Cell Stimulation Cocktail (containing phorbol 12-myristate 13-acetate (PMA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour tissue were mechanically dissociated on a 40 μm strainer and washed with PBS before transferring into 96-well round-bottom plates (Thermo Fisher Scientific). Samples were stained with 1:1,000 diluted LIVE/DEAD Fixable Stains (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were dissociated using TrypLE and seeded at 25,000 cells/well in V-bottom ultra-low attachment 96-well plates in 100 µL of Essential 6 medium (A1516401, Thermo Fisher Scientific) with 100 units/mL penicillin ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1,000 U/mL LIF added before replating 100µl per well of a U-bottom 96-well plate (Thermo Fisher Scientific, cat. 268200). For cytokine-free differentiation ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips were mounted on glass slides using Prolong Glass (Invitrogen), sealed with Covergrip sealant (Biotium ...
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bioRxiv - Microbiology 2020Quote: ... which collected aerial microbes on rotating sterilized glass slides lined with microtiter plate sealing film (Thermo Fisher Scientific, Waltham, MA). We immediately transferred all swabs and film to a microcentrifuge tube with 1 mL lysis buffer and garnet homogenization beads (Qiagen NV ...
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bioRxiv - Bioengineering 2020Quote: ... The CdWO4 scintillator plates were taken out from the 6-well plates and mounted onto a standard glass coverslip (0.1 mm thick, Fisher Scientific) upside down ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were plated onto 12-mm round glass poly-D-lysine coated coverslips placed in 24-well plates and transfected using lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... Cells were plated onto 12-mm round glass poly-D-lysine coated coverslips placed in 24-well plates and transfected using lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... were removed from the glass plates and incubated in ∼140 ml of TBE + 14 μl SYBR Gold Nucleic Acid Gel Stain (Invitrogen) for several minutes then destained in TBE for ∼10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... were seeded in 6-well plate with a cover glass inside and were transfected using lipofectamine 2000 (Thermo Fisher, #11668027) with mouse mEGFP M-Lip or mEGFP M-Lipxtal and mApple-Sec61b ...