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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Cell surface protein biotinylation and isolation were performed using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (Thermo Fisher Scientific, A44390) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total protein was extracted by radioimmunoprecipitation assay lysis buffer and protein concentration was determined by a BCA Assay Kit (Thermo Fisher Scientific, USA). Equal protein amounts (60 μg ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the membrane-associated proteins were transferred to a new tube followed by protein concentration determination by BCA assay (Thermo Fisher, A53225/A53226). For ubiquitin immunoprecipitation at 3.4 mg per sample ...
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bioRxiv - Cell Biology 2024Quote: HEK293T or HeLa Kyoto cells expressing the indicated tagged proteins were washed twice with ice-cold PBS and lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) containing protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of bEV was measured with Qubit™ Protein and Protein Broad Range (BR) Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA). The expression level of EV markers including CD9 ...
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bioRxiv - Cell Biology 2024Quote: ... the measured concentrations of various lipid classes (nmol/μL) were divided by protein concentration (μg/μL) that was assessed using BCA Protein Assay Kit (Thermo Fisher Scientific, 23225).
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bioRxiv - Neuroscience 2024Quote: ... The gradient fraction at the interface between the 23% and 10% Percoll containing synaptosomes were collected and protein concentration determined by Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, 23200).
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bioRxiv - Neuroscience 2024Quote: ... Raw cytokine values per well were normalized over nuclei number per well based on fluorescent staining of the fixed iAstrocytes in the plate or over protein content per well determined by Pierce™ BCA protein assay kit (Thermo Scientific, 23225). Pierce™ BCA protein assay was performed in a microplate as described in the user guide provided by Thermo Scientific.
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 16,000 x g for 15 minutes at 4°C and protein content determined by Pierce BCA protein assay kit (Thermo Fisher Scientific 23225). 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML ...
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bioRxiv - Neuroscience 2021Quote: ... 1:50 v/v) targeting the pre-synaptic active zone protein Bruchpilot (Hofbauer et al., 2009) and rabbit anti-GFP (Invitrogen, A-6455, 1:100 v/v) targeting mCD8::GFP ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were spun down with a tabletop centrifuge and the protein concentration was determined using the BCA protein assay kit (Thermo Fisher Scientific, Cat: A532225) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Whole-tissue sample lysates were batch process-assayed for total protein content using a BCA Protein Assay Kit (Thermo Fisher Scientific; Waltham, MA, USA). These lysates were used for Western blotting and enzyme assays described below.
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed by boiling in 1M NaOH and protein quantified using the Micro BCA™ Protein Assay (Thermo-Fisher Scientific Inc., Waltham, U.S.A.) and a VersaMax™ microplate reader (Molecular Devices LLC ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were determined using a BCA kit according to the manufacturer’s protocol (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Rockford, IL, USA). Citrate synthase (CS ...
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bioRxiv - Biochemistry 2020Quote: ... An aliquots of 5 μl of Page Ruler Plus pre-stained broad range standard containing a 9 protein ladder (protein composition in kDa of 10, 15, 25, 35, 55, 70, 100, 130 and 250; Thermo Fisher Scientific, CA, USA) was loaded as well ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets corresponding to 10 million cells were washed twice with ice-cold PBS and subjected to subcellular protein extractions using a Subcellular Protein Fractionation for Cultured Cells kit (Thermo Fisher Scientific, TFS #78840) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate was centrifuged at 14,000g for 5min at 4°C and protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, USA PI23227) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... and 3) verify functional cutaneous B2R antagonism via CVC and protein extravascularization (BCA protein assay of dialysate; Thermo Scientific, Waltham, MA, USA; Figure 1D). Increases in skin flux (vasodilation ...
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bioRxiv - Molecular Biology 2021Quote: Neurospora tissue was harvested at the specified circadian times by vacuum filtration and proteins were extracted using Protein Lysis Buffer with Halt™ Protease Inhibitor Cocktail diluted to a 1X concentration (Thermo Scientific™ 78430) 30 ...
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bioRxiv - Cell Biology 2021Quote: ... Each sample was measured for protein content using the PierceTM BCA protein assay kit-reducing agent compatible per manufacturer’s instructions (23250, Thermo Fisher Scientific, Waltham, MA, US). 100 ug of protein was used per sample ...
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bioRxiv - Physiology 2022Quote: The volume of tears per minute (µl / min) and the protein concentration in tears (mg / ml) using the BCA protein assay kit (Fisher Scientific, reference 10678484, France) were measured and expressed as the mean ± SD.
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bioRxiv - Cell Biology 2022Quote: ... the retinal cytosol and membrane proteins were separated using the Mem-PER™Plus membrane protein extraction kit (Cat #: 89842, ThermoFisher Scientific, Waltham, MA). The SDS sample loading buffer was added to the cytosol and membrane fractions ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA). The separated proteins were transferred to nitrocellulose membranes that were subse-quently blocked using 5% non-fat dry milk diluted in TBST (10 mM Tris-HCL ...
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bioRxiv - Physiology 2020Quote: ... All activities from tissue homogenates were normalized suitably by protein concentration using Pierce BCA protein assay (cat: 23227, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pathology 2019Quote: ... Co-immunoprecipitation of STAT3 with associated proteins was then performed by using Protein A/G Agarose beads included in the Pierce Classic IP Kit (26146; Thermo Fisher Scientific, Waltham, MA) followed by immunoblotting to detect immunoprecipitated HA/HA-STAT3 or co-immunoprecipitated PERK and MRTF-A.
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bioRxiv - Physiology 2020Quote: ... All microplate reader measurements were normalised to total protein content per sample using the Pierce™ BCA Protein Assay and Bovine Gamma Globulin assay (Thermo scientific, Loughborough, UK).
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bioRxiv - Systems Biology 2020Quote: Cellular protein contents were quantified by the Bicinchoninic Acid assay using a commercial kit (Pierce™ BCA Protein Assay Kit; Thermo Fisher, Waltham, MA). Stored samples (1 × 106 cells ...
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bioRxiv - Systems Biology 2020Quote: ... Titers were analyzed using a protein A HPLC (model 1100 HPLC, Agilent Technologies, Inc., Santa Clara, CA, protein A column model 2-1001-00, Applied Biosystems, Foster City, CA). The RNA libraries were mapped to the CHO genome89,90 using STAR aligner91 and processed to quantify gene expression counts with HTSeq-count92.
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bioRxiv - Biochemistry 2020Quote: ... The concentration of the bacteriocin was measured after each step of protein purification using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Molecular Biology 2019Quote: ... protein assay was done for extracted protein quantification using Pierce™ BCA Protein assay kit according to the manufacturer’s protocol (cat#23225, Thermo Fisher Scientific, MA, US). For western blotting ...
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bioRxiv - Plant Biology 2021Quote: ... Concentrations of proteins in plant leaves were determined according to the Pierce BCA Protein Assay Kit-Reducing Agent Compatible (Thermo Scientific, #23250, Rockford, IL).
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bioRxiv - Physiology 2019Quote: ... The total protein of the kidneys was extracted by centrifugation at 14,000g for 15 minutes at 4°C and protein concentration was determined using micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, Il, USA) according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg nuclear proteins or 7.5 μg cytosolic proteins were boiled in loading buffer with reducing reagents (Invitrogen, NuPAGE™ LDS Sample Buffer (4X), NP0007) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated in lysis buffer for 30 min at 4°C with continuous shaking and then centrifuged to collect clear supernatant for protein quantification was done using Pierce™ BCA protein assay kit (Thermo Fisher Scientific Inc.). Primary antibodies used ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) and protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... after which proteins were extracted using Protein Lysis Buffer with Halt™ Protease Inhibitor Cocktail diluted to a 1X concentration (Thermo Scientific™ 78430) 30 ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant from the cell lysates were transferred to new tubes and protein concentration of the samples was determined using a Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). The samples were then heated to 95°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... 5–15 µg samples were loaded per well (depending on protein of interest) next to 5 µl pre-stained protein ladder (PageRuler, Thermo Fisher Scientific, Waltham, USA). Proteins were separated by constant current (200 V ...
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bioRxiv - Cell Biology 2022Quote: ... The amount of BS present in liver extract was normalized to liver protein content as measured by bicinchoninic acid (BCA) assay (Pierce® BCA protein assay kit, Thermo Scientific, Waltham, Massachusetts).
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bioRxiv - Physiology 2022Quote: ... Supernatant was transferred to fresh tubes and the protein concentrations of the samples were measured with a Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23227). Samples were prepared for electrophoresis by denaturing 20μg of protein per sample in XT Sample Buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: ... liver was homogenized in RIPA buffer and protein levels were quantified using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Liver lysate and serum samples were analyzed by mass spectrometry for sphingolipid and fatty acid content at the metabolomics core at the Medical University of South Carolina ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by centrifugation and total protein extracts were obtained using M-PER™ Mammalian Protein Extraction Reagent (Thermo-Fisher Scientific, CA, USA) supplemented with pierce protease kit and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Dried residues were resuspended in MilliQ water and total protein content of each homogenate was measured with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). 20 µl of each sample was injected into a 25 cm x 4.6 mm C18 Nucleosil column with 5-µm particles (Interchim ...
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bioRxiv - Cancer Biology 2024Quote: ... cells scraped off and transferred to a 1.5 ml tube for cell lysis before clearance by centrifugation and protein concentration measurement using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) following manufactureŕs instructions and the FLUOstar Omega reader ...
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bioRxiv - Biophysics 2024Quote: ... HPG labeling of proteins was detected using the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit (Life Technologies, cat. #C10429), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... and the concentration of total protein in cell extracts was determined using the Qubit® protein assay kit and the Qubit® 3.0 fluorometer (Thermo Fisher Scientific™). Cell culture supernatants were used purely ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein content in BAL fluid after cell removal was assessed using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer’s instructions.