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Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). The lysates were loaded in the amount of 5 µg of total protein in Laemmli sample buffer onto a 6% (v/v ...
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bioRxiv - Neuroscience 2024Quote: Total protein levels of all CSF samples were determined by use of a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA). CSF Aβ40/Aβ42 and total tau (t-tau)/tau phosphorylated at threonine-181 (p-tau ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit; #23225, Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2024Quote: ... 10-30 µg of protein was loaded onto precast gel (NP0321, NuPAGE™ 4- 12%, Bis-Tris, Mini Protein Gels, ThermoFisher Scientific, USA) and resolved at 135 volts for 90 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 30 μg of protein fraction for each sample was separated by electrophoresis using NuPAGETM 4-12% Bis-Tris Protein Gel (ThermoFisher Scientific, Waltham, MA). Proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins of AGS cells were isolated with the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, United States). Briefly ...
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bioRxiv - Immunology 2024Quote: ... The final spike protein concentration was determined by measuring absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Proteins were dispensed into 0.5 ml aliquots and stored at -80°C.
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content was determined using the bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with an automated KoneLab instrument (Thermo Scientific, Vantaa, Finland).
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bioRxiv - Cell Biology 2024Quote: ... The Bradford assay was used to quantify the total protein content of lysates (Bradford, 1976) using Coomassie Protein reagent (Thermo Scientific, cat: 1856209). Lysates were stored at −80° C if not immediately being used for SDS-PAGE sample preparation.
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in Ripa Buffer and total protein was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227) for data normalization.
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bioRxiv - Systems Biology 2024Quote: ... The detection of protein synthesis was performed according to the protocol provided by the Click-iT® Plus OPP Protein Synthesis Assay Kits (Thermo Fisher, C10456). In brief ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of the membrane fractions and whole cell lysates were measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). Myc-tag immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Cancer Biology 2023Quote: The amount of proteins was quantified for each sample and time point using Pierce™ Bicinchoninic Acid (BCA) Protein Assay (Thermo Fisher Scientific). Samples were loaded at 10 µL per well in duplicate ...
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bioRxiv - Neuroscience 2023Quote: ... Sample concentrations were quantified based on the standard curve and normalized to total protein obtained through BCA protein assay kit (Thermo Fisher Scientific, 23225) We performed 5-6 independent experiments with 2-3 technical replicates per experiment that were averaged within each experiment.
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bioRxiv - Biochemistry 2022Quote: All NativePAGE analyses were conducted in an Invitrogen XCell SureLock using NativePAGE™ 4 to 16% bis-tris mini protein gels and NativeMark Unstained Protein Standard from Fisher Scientific (USA) with 1x running buffer made from 10x Tris/Glycine Buffer from Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Microbiology 2022Quote: ... Estimation of protein purity and quantity were achieved with SDS-PAGE and a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL), respectively.
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bioRxiv - Cell Biology 2023Quote: ... Samples were centrifuged for 10 min at 10,000 times gravity (ξ g) at 4 °C insoluble materials and total protein concentration was measured by the PierceTM BCA protein assay (Thermo Fisher Scientific). 15-20 µg of samples was prepared in Laemmli buffer and run in 8% SDS-PAGE before being transferred onto nitrocellulose membrane at 320 mA for 2.5 h ...
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bioRxiv - Immunology 2023Quote: The concentration of total protein in each nasal swab was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher, Cat No. 23225) according to the manufacturer’s microplate procedure ...
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bioRxiv - Immunology 2023Quote: ... Total protein in BALF was determined by the bicinchoninic acid (BCA) colorimetric assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, #23227).
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined using the Pierce 660 nm Protein Assay Reagent together with the Ionic Detergent Compatibility Reagent (Thermo Scientific, Waltham, MA).
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bioRxiv - Immunology 2023Quote: ... Total protein concentrations of 24-hour GD18 urine was determined using a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the lysates were centrifuged for 15 min at 16,000 g and the supernatant containing the proteins were subjected for protein determination using a commercial Bicinchoninic acid assay (BCA) kit (Thermo Fisher, Karlsruhe, Germany). 50 g of proteins were heat desaturated in SDS-sample buffer (supp ...
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bioRxiv - Immunology 2023Quote: ... The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Thermo Scientific, USA, #23225).
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bioRxiv - Bioengineering 2023Quote: ... was used for separation of the proteins by molecular weight and then separated proteins were transferred in iBot 2 PVDF membrane (Thermo Fisher Scientific, USA). Anti-Epo polyclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Biochemistry 2023Quote: ... The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific, Waltham, Massachusetts, USA). Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... An acetone-based protein precipitation was performed followed by tryptic digest and desalting of digested proteins via stop-and-go extraction tips (Thermo Fisher Scientific, USA) as described (Beyer et al ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Mixed and protein fractions were pre-cleared of IgG from FBS with 100µL of protein G coated magnetic Dynabeads (Thermo Fisher 10003D Lot# 00715594) overnight at 4°C with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein molecular masses were estimated relatively to the electrophoretic mobility of co-transferred prestained protein marker PageRuler™ Prestained Protein Ladder (Cat#:26616, Thermo Fisher Scientific). The following primary antibodies were used ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatants were transferred to clean tubes and their protein concentrations were measured using a BCA Protein Assay Kit (Pierce Thermo Scientific, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... an SDS Bis-Acrylamide gel was loaded with 20 µg of protein from each sample and a standard protein marker (PageRuler Plus, cat. No. 11852124; Thermo Fisher Scientific, USA). The proteins were transferred to FL-PVDF membranes (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Purification yield and protein integrity were analyzed by SDS-PAGE followed by Coomassie staining according to manufacturer instructions (PageBlue Protein Staining Solution; Thermo Fisher Scientific, 24620). Protein concentration was determined with NanoDrop measurements.
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bioRxiv - Microbiology 2023Quote: ... which was quantified using both optical density at 600 nm (OD600) and total protein content estimated using a BCA protein assay kit (Thermo Scientific, MA, USA). For quantification of the expression of the arsM gene ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected and protein concentration was determined using the PierceTM BCA protein assay kit following manufacturer’s instructions (Thermo Fisher Scientific, Massachusetts, USA). Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... The top 14 high-abundant blood plasma proteins (HAP) were depleted using single-use High Select™ Top 14 Abundant Protein Depletion Midi Spin Columns (#A36371, Thermo Fisher Scientific). These included albumin ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard, Thermo Fisher) on opposite ends of the gel ...
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bioRxiv - Neuroscience 2023Quote: ... Cytokines and ApoE levels were normalized to the total amount of protein determined by BCA protein assay (Thermo Scientific, Pierce, catalogue no. 23225).
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bioRxiv - Molecular Biology 2023Quote: ... PRP pellets were lysed in lysis buffer and their protein concentration was measured using Pierce BCA protein assay kit (Thermo Scientific, Cat. 23225) and a Tecan Infinite 200 plate reader (Tecan Group Ltd. ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant from each sample was then determined using the Pierce BCA protein assay kit (Thermo Scientific, Catalogue #23225) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration from whole cell lysate was measured by BCA assay using Pierce™ BCA Protein Assay Kit (Cat# 23227, Thermo Scientific™) as per manufacturer protocol and 40 µg of protein per well was separated on 12% SDS PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 µg of total protein fraction for each sample was separated by electrophoresis using NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific). Proteins were transferred to a polyvinylidene difluoride membrane using an XCell SureLock Mini-Cell Electrophoresis System (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... An aliquot of each batch of ACM was thawed and used to measure the protein content using the Pierce BCA protein assay kit (Thermo Fisher, Cat# 23225).
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bioRxiv - Cell Biology 2023Quote: ... Total protein was determined by the bicinchoninic acid method according to the manufacturer’s instructions (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia).
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bioRxiv - Cell Biology 2024Quote: ... The concentration of bEV was measured with Qubit™ Protein and Protein Broad Range (BR) Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA). The expression level of EV markers including CD9 ...
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bioRxiv - Cell Biology 2024Quote: HEK293T or HeLa Kyoto cells expressing the indicated tagged proteins were washed twice with ice-cold PBS and lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) containing protease and phosphatase inhibitors ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the membrane-associated proteins were transferred to a new tube followed by protein concentration determination by BCA assay (Thermo Fisher, A53225/A53226). For ubiquitin immunoprecipitation at 3.4 mg per sample ...