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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The total protein concentration of the EVs was measured using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) to estimate the concentration and the yield ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was collected from the samples and BCA Assay was performed to determine the protein content (Pierce™ BCA Protein Assay Kit, Thermo Scientific, 23223).
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bioRxiv - Developmental Biology 2021Quote: ... The supernatants were transferred into new tubes and protein concentrations were quantified using the Pierce BCA Assay Protein kit (Thermo Fisher Scientific – 23225). 60μg total protein were mixed with SDS gel loading buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Fifty micrograms of total denatured protein were then separated by SDS-PAGE using precast NuPAGE 4-12% Bis-Tris protein gels (Invitrogen, Waltham, MA, USA) followed by electrotransfer onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... The results were normalized using total protein content of the cells that was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Experiments have been performed in triplicates in five independent experiments.
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bioRxiv - Microbiology 2020Quote: ... sera were harvested and IgGs were purified on protein A and labeled with Alexa Fluor™ 647 using the protein labeling kit (Thermo Fisher Scientific) as recommended by the manufacturer.
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bioRxiv - Cancer Biology 2020Quote: ... Protein sizes were determined using the Precision Plus Protein Dual Color Standards marker (Catalog Number 161-0374) from Life Technologies (Carlsbad, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... the total protein concentration was quantified and normalized in all samples using the Pierce BCA protein assay kit (ThermoFisher Scientific, Waltham, MA, USA). Results were expressed as median fluorescence intensity (MFI).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations of soluble worm extracts were determined using the Pierce™ BCA™ Protein-Assay (Art. No. 23225, Thermo Scientific™) and an Infinite® M200 Pro plate reader (Tecan) ...
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bioRxiv - Neuroscience 2022Quote: ... v/v) before LC-MS/MS analysis according to the total protein content (quantified using BCA protein Assay Kit (Thermo Scientific, Massachusetts, USA).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific, Waltham, MA). After transfer to nitrocellulose ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the supernatant was collected and the cleared lysate protein concentration was quantified using the BCA protein assay kit (Thermo Scientific, Pierce Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein concentration in lysates (supernatants) was determined using the BCA method (Pierce™ BSA Protein Assay Kit, Thermo Fisher Scientific, Waltham, MA). Proteins (20-30 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... soluble nuclear and chromatin-bound protein extracts were prepared by stepwise separation using Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific, 87790). For this purpose ...
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bioRxiv - Biochemistry 2022Quote: ... combined protein concentrations of complex I and MSP2N2) were determined using the Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific). For the sample subjected to cryo-EM analyses ...
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bioRxiv - Biochemistry 2022Quote: ... All samples were normalised to total protein concentration (mg) in the sample by using the Pierce™ BCA total protein quantification assay (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... O2 and H2O2 flux rates were normalised to total protein (mg) in chamber by conducting Pierce™ BCA total protein quantification assay (Thermo Fisher Scientific) either directly from homogenate sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). Lysates containing 5 μg of total protein in Laemmli sample buffer were loaded onto a 6% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a fresh tube and protein was quantified using a Pierce BCA Protein assay kit (Thermo Fisher; Cat. #23225).
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bioRxiv - Microbiology 2020Quote: ... aliquots were diluted 1:2 and 1:5 in 2M urea solvent and their total protein concentration was measured using a Pierce Bicinchoninic Acid Protein Assay (Thermo Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Concentration of the final purified protein solution was quantified using the Pierce™ Bradford Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Bioengineering 2019Quote: ... 1mg of total protein extracts were pre-cleared by incubating with Pierce™ Protein A/G Plus Agarose (Thermo Scientific cat. nr. 20423) for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant from each tube was isolated and protein concentration for each sample was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL). 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad ...
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bioRxiv - Cell Biology 2022Quote: ... the concentration of total soluble proteins of each sample was determined using BCA™ Protein Assay (Thermo Scientific™ Pierce™, Rockland, IL). To run a SDS gel ...
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bioRxiv - Physiology 2019Quote: ... Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific, #1856209). Cell lysate was incubated with Anti-FLAG M2 antibody for 1 h and immunoprecipitated (IP ...
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bioRxiv - Genomics 2020Quote: ... cell debris was removed by centrifugation at 10000rpm for 10 min and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were prepared for SDS-PAGE (30μg loaded per well) ...
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bioRxiv - Developmental Biology 2019Quote: ... Amylase levels were normalized to the total protein content determined by a BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, 23225).
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (15-50 µg) were subjected to SDS-PAGE using NuPAGE 4-12% Bis-Tris Midi Protein Gels (Invitrogen, Carlbad, MA, USA) and subsequently transferred to Polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2019Quote: Ab019 and Ab161 were purified by incubation with Protein G beads and their concentrations were determined by BCA protein assay kit (Thermo Scientific, IL, USA). According to the manufacturer’s manual ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was transferred into a fresh tube and protein concentration determined using the Pierce™ BCA protein assay kit (Thermo Scientific, #23225). 550ug protein from each sample was diluted to 500ul and mixed with 3 ug ACTN2 antibody (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific, Dreieich, Germany). The following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Physiology 2019Quote: ... The supernatant was collected following centrifugation at 8,000 g for 10 minutes and protein concentrations were determined using the Pierce™660 nm protein assay reagent (Thermo Scientific, Waltham, USA). Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels and transferred to polyvinylidene diflouride membranes ...
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bioRxiv - Biochemistry 2020Quote: ... Dithiothreitol was removed from the protein using PD-10 columns and the protein was labelled with Alexa Fluor™ 647 C2 Maleimide (Thermo Fisher Scientific) (Hoernke et al. ...
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bioRxiv - Cancer Biology 2019Quote: The EV fraction or the transfected PCa cells was measured for protein content using the Micro BCA Protein Assay kit (Thermo Scientific, Wilmington, DE). Equal amounts of protein were loaded onto 4-20% Mini-PROTEAN TGX gels (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... The supernatant was collected following centrifugation at 8,000 g for 10 minutes and protein concentrations were determined using the 660 protein assay (Thermo Fisher Scientific, Waltham, MA). Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... Ovalbumin was labelled with Alexa Fluor 647 with the microscale Protein labelling kit (Alexa Fluor 647 Microscale Protein Labelling Kit (Life Technologies, Darmstadt, Germany). Intratracheal instillation of 2.5 μg of OVA dissolved in 20 μl PBS was performed and held over 6 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was determined for each sample using a Pierce reducing agent-compatible BCA protein assay kit (Thermo Fisher Scientific, cat. no. 23250).
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bioRxiv - Cell Biology 2020Quote: ... Resulting eluate was tested for purity and protein amount on a reducing SDS-PAGE and stained with Imperial protein stain (Thermo Fisher, Cat# 24617). Elutions were then combined and protein concentration was quantified by a Bradford assay (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of each total lysate (mixture of murine and cryptococcal proteins) from the protein isolation step was run on a 12% NuPAGE™ tris-acetate gel (Thermo Fisher Scientific) two centimeters into the gel prior to Coomassie R250 staining and water de-staining for subsequent proteomic analysis.
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bioRxiv - Neuroscience 2022Quote: ... for 10s/sample on ice and protein concentration was determined using Pierce BCA protein assay kit following manufacturer’s instruction (Thermo Fisher Scientific cat#23227). 20μg to 30 μg of total protein from each lysate was combined with loading buffer (4X ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). OCR measurements were normalized to the protein concentration in each well.
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bioRxiv - Genomics 2022Quote: ... for 30 minutes at 4°C and supernatant was retained and protein concentration quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). A total of 25-35μg proteins were loaded onto 12.5% Tris-HCl gel for SDS-PAGE analysis ...
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bioRxiv - Microbiology 2022Quote: ... The ribosome pellet was suspended in RIPA buffer and the amount of protein was quantified from both the ribosomal fraction as well as the whole lysate by Pierce™BCA Protein Assay (Thermo Fisher Scientific). Samples were then separated by 4-12% polyacrylamide Bis-tris gel electrophoresis (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration of NAD+ or NADH extracts was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL) to normalize NAD(P)H and NAD(P)+ concentrations.
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bioRxiv - Microbiology 2022Quote: ... fluorescent FFE skin cells were collected from the FF using forceps and protein was collected using M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) to be used in immunoblotting or frozen and processed by the University of Illinois Protein Science Laboratory for mass spectrometry (MS)-based analyses.
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bioRxiv - Molecular Biology 2022Quote: ... JPH2 or JCN and their interacting proteins were co-precipitated by using an immunoprecipitation kit (Dynabeads Protein G; Life Technologies, Inc., ON, Canada) in tissue or cell lysates according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA). Activity was compared to the solvent control samples (set at 100% activity) ...
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bioRxiv - Molecular Biology 2023Quote: ... The whole cell lysate were obtained with RIPA and the protein concentration was measured using BCA protein assay kit (23225, Thermo Scientific, MA, USA). Monoclonal antibody anti-DNMT1 (ab188453 ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized samples were centrifuged at 14,000 rpm for 15 min at 4°C and the protein concentration of the supernatant was determined by Pierce BCA Protein Assay (ThermoFisher Scientific, cat# 23227). Samples were diluted and supplemented with SDS sample buffer (50 mM Tris-HCl pH 6.8 ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) was added on each pellet and total protein quantification was performed using BCA Protein Assay kit (Pierce™, Thermo Fisher Scientific). Before immunobloting analysis ...