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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Sections were washed 3× for 5 min each in PB and mounted with Prolong Gold (Thermo Fisher) and Deckglaser cover glass (Cat#NC1776158) ...
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bioRxiv - Cell Biology 2022Quote: ... 5′-RACE and 3′-RACE were done using the GeneRacer method according to the manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... they were concentrated from 40 μl to 5 μl during 2h at low temperature using speedvac (Thermofisher). Small RNA libraries were then built with Illumina TruSeq Small RNA Sample kit following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... stained with 8 µl of 5 mg/ml Alexa fluor 488-conjugated phalloidin (Thermo Fisher Scientific, A12379), and incubated in a rotary platform overnight at 4°C in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... then transfected for 5 hours with indicated plasmids in 2 mL Opti-MEM (Invitrogen, Cat. #31985-088) using Lipofectamine 3000 transfection kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μm thick paraffin sections were mounted on ProbeOn™ slides (Thermo Fisher Scientific #15-188-51)) ...
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bioRxiv - Genomics 2022Quote: ... was added in 50 μl-reaction system supplied with 5 μl Buffer Tango (10X, Fisher Scientific, BY5) and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... K562 cells (ATCC CCL-243) were maintained at 37°C and 5% CO2 in RPMI (ThermoFisher 11875119) with 10% FBS (Corning 35015CV) ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′) and KAPA HiFi HotStart ReadyMix (Thermo Fisher Scientific), cleaned up using RNAClean XP SPRI beads three times ...
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bioRxiv - Immunology 2022Quote: Spleen cells (3.0 × 107) were stained with 5 μM 5,6-carboxyfluorescein succinimidyl ester (CFSE; Molecular Probes, USA) in phosphate-buffered saline with 0.1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cells were plated at the density of 5×10⁵ cells/cm² after disassociation by TrypLE express (Invitrogen) into single-cell suspension ...
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bioRxiv - Genomics 2022Quote: ... Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 minutes (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.
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bioRxiv - Molecular Biology 2022Quote: Arabidopsis seedlings of 5 days after germination (DAG) were fixed in 4% methanol-free formaldehyde (Thermo Scientific) diluted in nuclease free 1 x PBS (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) and antibiotics (0.1 unit penicillin, 0.1 mg.mL-1 streptomycin, GIBCO) at 37 °C in a humidified 5% CO2 incubator.
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bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDM) were differentiated over 5-7 days in DMEM (Gibco, 11960044 or 11885092), 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... and 8% CO2 following the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... After 4-5 h bacteria were collected by centrifugation (5000 rpm, SLA 3000 rotor, ThermoFisher Cat#07149) for 15 minutes and resuspended by gentle trituration in ice-cold 50 ml of TBS Buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: Hela S3 cells were cultured in suspension with a 5 Liter T-flask in SMEM (Thermofisher Scientific) supplemented with 10% fetal calf serum (SigmaAldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were trapped on a μPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm × 5 mm, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with 5% nonfat dried milk and incubated with CAMKIIα (1:1000, Invitrogen PA5-84083) and VINC (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... and then stained for markers at 5 μg/ml for IL-17A-FITC (Thermofisher, 11-7177-81) and IFNg-PE (Thermofisher ...
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bioRxiv - Plant Biology 2023Quote: ... Primers for qRT-PCR were designed using Primer Prime Plus 5 Software Version 3.0 (Applied Biosystems, USA). For internal reference ...
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bioRxiv - Immunology 2022Quote: Enriched Treg and non-Treg cells were stained with 5 μM of CellTrace Violet Proliferation Marker (ThermoFisher) for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher. mAbs for chemokine receptors (i.e ...
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bioRxiv - Neuroscience 2022Quote: ... cultured in Opti-MEM supplemented with 5% FBS and 200 μM Sodium pyruvate (all from Thermo Fisher). Lentivirus was generated using a 3rd generation packaging system ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated at 37°C/5% CO2 and passaged using TrypLE Express (GIBCO; Gaithersburg, MD, USA) as a dissociation agent once 70-90% confluent ...
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bioRxiv - Physiology 2022Quote: ... and gene expression was quantified utilizing Taqman qPCR probes and QuantStudio 5 Real-Time PCR System (ThermoFisher). Data were analyzed using Excel and GraphPad PRISM ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as the eluate from RNA-ChIP were treated by 5 U of TURBO-DNase (Ambion) 1 h a 37°C prior purification by acidic phenol/chloroform method and ethanol-precipitation with 5µg of Glycoblue (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant were purified by acidic phenol/chloroform method and ethanol-precipitation with 5 µg of Glycoblue (Ambion) as carrier ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... heated for 5 minutes at 95C and separated on 4-12% NuPAGE® Bis-Tris gel (Invitrogen) by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore® ...
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bioRxiv - Molecular Biology 2022Quote: ... WT cells were transfected with 250□Jµl Opti-MEM that contained 5□Jµl Lipofectamine 2000 (Thermo Fisher) and□J2.5µg sgRNA-pX330-mCherry plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated across a 2.1 mm x 5 cm C18 column (1.9 µl Hypersil Gold, ThermoFisher) with a linear gradient of 4% - 40% CH3CN and 0.3% formic acid ...
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bioRxiv - Molecular Biology 2022Quote: ... then treated samples with RNase A (5 U/ml) and TURBO DNase (1 U/ml, Thermo fisher) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5% CO2 in a humidified cell culture chamber (Heracell™ VIOS 160i CO2; Thermo Scientific™).
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bioRxiv - Bioengineering 2022Quote: ... 5’ppp-RNA was prepared by IVT in the absence of ATP using MEGAshortscript T7 kit (Thermofisher) and DNA template ...
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bioRxiv - Biophysics 2022Quote: ... Both cell lines were cultured at 37 °C and in 5% CO2 atmosphere in DMEM (Life Technologies) medium containing 10% heat-inactivated FBS (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... and primer pairs were run in triplicates on a QuantStudio 5 Real-time PCR instrument (Applied Biosystems). Primers were designed using the NCBI/Primer Blast tool (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μg RNA were reverse-transcribed in a reaction containing 1 μl dNTP (10 mmol/l; Invitrogen), 0.5 μg oligo(dT ...
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bioRxiv - Physiology 2022Quote: ... Fresh retinas were minced and incubated in 5 ml DMEM containing 200 U/ml collagenase I (Invitrogen) for 45 mins at 37°C with occasional shaking followed by filtering through a 40 μm nylon mesh ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μm sections were taken from the FFPE tissue using a microtome (Thermo Scientific HM355S; MX35 blades). For the Chromium Fixed RNA Profiling (scFFPE-seq ...
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bioRxiv - Cell Biology 2022Quote: CHO cells were kept at 37°C and 5% CO2 in OptiMEM™ + Glutamax™ (Life Technologies) supplemented with 10% heat-inactivated endotoxin-low FCS ...
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bioRxiv - Pathology 2024Quote: ... The qPCR reactions were performed in triplicates using a QuantStudio 5 Real- time PCR System (Thermo Fisher) using the following thermocycling conditions ...
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bioRxiv - Physiology 2023Quote: ... Fixation was quenched 1 ml of 1.375 M glycine (Cat# BP381-5, Thermo Fisher Scientific, Waltham, MA) with gentle nutation for 5 mins at room temperature ...
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bioRxiv - Microbiology 2024Quote: Reverse transcription quantitative PCR analysis was performed on QuantStudio 5 Real-Time PCR system (Thermo Scientific, USA) using the primer set from E or RdRp Charité (IDT Coralville ...
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bioRxiv - Microbiology 2024Quote: ... CRL-3216) were cultured at 37⁰C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Genetics 2024Quote: ... Slides were covered by mounting medium with nuclear counterstain DAPI (Thermofisher, catalog number D1306, 5 ng/ml). Immunohistochemistry for zebrafish was performed as described20 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR analyses were performed with 5 µl of cDNA using SYBR© Green fluorophore (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested using pre-mixed Earle’s Balanced Salt Solution (EBSS) with 5 mM EDTA (Life Technologies) and centrifuged at 3000 rpm (10 min ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were cultured in a 95% air/5% CO2 Thermo Forma incubator (Thermo Scientific, Waltham, MA) at 37°C ...