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Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Reactions were conducted with 5 μL RNA and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems) with 500 nM primers and 200 nM probes ...
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bioRxiv - Cell Biology 2021Quote: ... Permeabilized cells were washed with PBS (2x) and blocked with 5% goat serum (Invitrogen, Grand Island, NY) for 45 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 SCID tumor samples and the mT3-2D cell line using the PureLink RNA Mini Kit (Ambion). RNA concentration and quality were assessed using the Agilent BioAnalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with DAPI for 5 min in PBS and washed twice before mounting with Prolong Gold (Invitrogen). Cells were imaged on a Zeiss Imager.Z2 microscope using the ApoTome.2 structured illumination platform ...
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bioRxiv - Developmental Biology 2021Quote: ... Japanese quail skins were incubated at 37°C with a 5% CO2 atmosphere (Thermo Scientific Midi 40). Explant were cultured at 37°C in 5% CO2 atmosphere (Thermo Scientific Midi 40) ...
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bioRxiv - Cell Biology 2019Quote: MCF10A human mammary epithelial cells (ATCC) were grown at 37° and 5% CO2 in DMEM/F12 (Gibco) with 5% horse serum (HS ...
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bioRxiv - Genetics 2021Quote: ... 5 U of the required restriction enzyme (BsaI/Eco31l, #ER0292, Thermo Fisher, or BpiI, ER1011, Thermo Fisher), and 400 U T4 DNA ligase (M0202 ...
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bioRxiv - Genetics 2021Quote: ... 5 U of the required restriction enzyme (BsaI/Eco31l, #ER0292, Thermo Fisher, or BpiI, ER1011, Thermo Fisher), and 400 U T4 DNA ligase (M0202 ...
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bioRxiv - Genetics 2021Quote: ... The reaction was performed using SYgreen Blue Mix (PCRbio # PB20.15-20) and a QuantStudio 5 (Applied Biosystems) thermal cycler.
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bioRxiv - Genetics 2019Quote: 2.106 CEM cells were centrifuged for 5 minutes at 1200 rpm and washed in PBS 1X (Gibco). They were then transfected by nucleofection with 5 μg DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... depending on the sorting round) and 5 µg/mL mouse anti-c-myc (clone 9E10, Thermo Fisher), followed by a secondary incubation with 20 µg/mL goat anti-mouse IgG-Alexa Fluor 488 and 10 µg/mL streptavidin-Alexa Fluor 647 (both Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn (Thermo Scientific, Illkirch-Grafenstaden/France) and separated on a 75 μm × 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2020Quote: ... Glycosylated RboP hexamers were coupled to beads by adding 5×107 Dynabeads M280 Streptavidin (Thermo Fisher Scientific) to the individual glycosylation reaction mixtures ...
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bioRxiv - Microbiology 2020Quote: ... MEFs were maintained at 5% CO2 and 37°C in high-glucose Dulbecco’smodified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl of sample was loaded onto the trap using an Easy nLC-1000 (Thermo Fisher Scientific). Each sample was separated using the Easy nLC-1000 with a binary mobile phase gradient to elute the peptides ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Immunology 2020Quote: ... T cells were resuspended in 1X PBS and stained with CFSE (Invitrogen, #C34554, working concentration: 5 μM). T cells were incubated at 37°C for 20 mins and then washed twice with an excess of media ...
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bioRxiv - Microbiology 2021Quote: ... The 10-μL PCR mixture comprised 5 μL of TaqMan® Universal Master Mix II (Applied Biosystems) with uracil-N-glycosylase (UNG) ...
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bioRxiv - Microbiology 2021Quote: ... U937 monocyte cells were cultivated at 37°C in 5 % CO2 in RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10 % heat-inactivated foetal calf serum (HyCloneTM) ...
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bioRxiv - Immunology 2020Quote: For cell proliferation assays splenocytes freshly isolated from P14 mice were labelled with 5 μM CFSE (Invitrogen) at 37 °C for 20 min in RPMI complete media (described above) ...
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bioRxiv - Immunology 2020Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Lipid droplets and nuclei were stained with 0.2% Triton-X100/PBS containing 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503, 5 μg/ml; Thermo Fisher)88 ...
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bioRxiv - Immunology 2020Quote: ... Samples were acquired on an LSRFortessa 5 with stained UltraComp eBeads Compensation Beads (Invitrogen #01-2222-41) as compensation controls.
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bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
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bioRxiv - Genomics 2020Quote: ... (RV-5′-GCCGTGGGTACTGAGAAAG-3′) and the fragments were bi-directional sequenced using (3130 Genetic Analyser; Applied Biosystems). Further the results were compared with the reference sequence of ARHGEF40 gene using NCBI-BLAST program and the chromatogram was analysed using Chromas lite (2.1 ...
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bioRxiv - Cell Biology 2021Quote: ... (5 μg with Takara Clontech Xfect in NRVMs, 2.5 ug and 7.5ul Lipofectamine LTX reagent, Thermo Fisher’s Lipofectamine LTX & PLUS Reagent ...
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bioRxiv - Genetics 2020Quote: ... 5% CO2 in Dulbecco modified Eagle medium (DMEM; GE) supplemented with 10% fetal bovine serum (FBS; GIBCO) and 1% antibiotics (penicillin 100 U/ml ...
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bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-10A cells were cultured in DMEM-F12 (11320-033) with 5% horse serum (Gibco, 16050-122), hydrocortisone 0.5 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were then given intraplantar injection of 10 μL of 5% neutral-buffered formalin (NBF) (Fisher Scientific) in 0.9% saline (Millipore Sigma) ...
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bioRxiv - Immunology 2022Quote: ... Peptides was were loaded via an Acclaim PepMap 100 trap column (C18, 5 μm, 100Å; Thermo Scientific) onto a Waters BEH C18 column (100 μm × 100 mm ...
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bioRxiv - Microbiology 2022Quote: ... blotted for 4-5 s and plunge-frozen into liquid ethane in a Vitrobot Mark IV (ThermoFisher), while the blotting chamber was maintained at 100% humidity at 10 ºC ...
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bioRxiv - Immunology 2022Quote: ... Probe: 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-NFQ-MGB-3’ (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... centrifugation (table top max speed; 5 mins) before they were run on 4-12% polyacrylamide gels (Invitrogen). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... NSCs were cultured at 37°C/5% CO2 in serum-free media: DMEM/F12 GlutaMax (10565018; Invitrogen) with B27 (1:50 ...
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bioRxiv - Molecular Biology 2022Quote: ... In vitro transcribed RNA (15 pmol) was 5’ dephosphorylated using FastAP thermosensitive alkaline phosphatase (Thermo Fisher Scientific) in 20 μl reaction containing 1x FastAP buffer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: HEK293T and HeLa cells were cultured at 37 °C and 5% CO2 in high glucose DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... all syntheses were cleaved from resin with 5 mL of 92.5:2.5:2.5:2.5 trifluoroacetic acid (>95%, Fisher Scientific):DI water:triisopropylsilane (98% ...
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bioRxiv - Bioengineering 2022Quote: ... at 37? and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) / F-12 (Gibco, Thermo Fisher, USA) containing 10% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... at 37? and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) / F-12 (Gibco, Thermo Fisher, USA) containing 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection with 5 ug total DNA in a 6 well plate was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the equivalent of 5-10 adult worms was loaded into 4-12% NuPAGE Bis-Tris gels (Invitrogen). Proteins were transferred overnight to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2022Quote: ... The tumoroids were centrifuged at 300 g for 5 minutes and then digested with TrypLE solution (Gibco) for 5 min at 37 ° C ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantify mitochondrial membrane potential cells were incubated with 20 nM MitoProbe DiIC1(5) (Thermo Fisher, M34151). To quantify mitochondrial oxidative potential cells were incubated with 100 nM MitoTracker Red CM-H2XRos (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed three times with 1×PBS for 5 min and stained with DAPI (ThermoFisher Scientific) for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... with a 5 eV slit width and a Falcon 4 or Falcon 4i direct electron detector (ThermoFisher). Images were collected at a pixel size of 0.745 Å/pix or 0.951Å/pix with a cumulative dose of 40e−/Å2 for untilted ...
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bioRxiv - Bioengineering 2022Quote: ... Real-time PCR was performed in 5 μL reactions using the PowerUp SYBR Green Mastermix (ThermoFisher #A25742) and a QuantStudioI 6 Flex System (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: Samples were denatured and reduced using 5× Lane Marker Reducing Sample Buffer (Thermo Scientific, Rockford, IL, US), boiled at 95°C for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 45 minutes using a blocking buffer containing DPBS with 5% goat serum (ThermoFisher #16210064) 0.05% sodium azide (Sigma #S2002 ...
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bioRxiv - Biochemistry 2022Quote: ... anti-rabbit fluorescent secondary antibodies (Alexa Fluor 488; 1:1000) and Hoechst 33342 (5 μg/ml; Invitrogen) at room temperature for 1 hr ...