Labshake search
Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
-
bioRxiv - Immunology 2022Quote: ... IVT was then performed using MEGAscript® T7 Transcription Kit (Thermo Fisher Scientific, AMB-1334-5) according to the manufacturer’s instructions with modifications as using the CleanCap® Reagent AG (TriLink ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Cells were grown in a humidified 37°C (5% CO2) atmosphere in MEM α (ThermoFisher, #32571101) supplemented with 5% HyClone fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2022Quote: The samples were counterstained using 5 ng ml-1 DAPI dissolved in SlowFade Gold (Invitrogen, USA) and covered with a #1.5 high precision coverslip (Marienfeld ...
-
bioRxiv - Microbiology 2022Quote: ... cells were pelleted and resuspended in 50 ul of 5 µg/ml FM4-64 (Molecular Probes). Three microliters of suspension were placed on a microscope slide and immobilized with a poly-L-lysine-treated coverslip.
-
bioRxiv - Molecular Biology 2022Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
-
bioRxiv - Microbiology 2022Quote: ... Protein was then crosslinked to beads with 5 mg/ml DTSSP (Thermo Fisher Scientific, Rockville, MD) in binding buffer for 45 min at RT ...
-
bioRxiv - Microbiology 2022Quote: Genomic DNA was assayed by qPCR on the QuantStudio 5 – Real-Time PCR System (Thermo Fisher) using TaqMan Universal PCR Mastermix (Thermo Fisher) ...
-
bioRxiv - Immunology 2022Quote: ... 2-5×106 cells were incubated in 15 μl Indo-1 solution in 1ml IMDM (Gibco) + 1%FBS (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... RNA was isolated by adding 5 volumes of Trizol LS Reagent (Life Technologies, Carlsbad, CA, USA) followed by a 5 min incubation at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... Blots were washed 3x 5 min in TBST and 2mL each SuperSignal Substrate (Thermo Fisher Scientific) reagent was added to blot and incubated for 5 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5 ug of RNA was converted to cDNA using Maxima H Minus Reverse Transcriptase (ThermoFisher, EP0751) according to manufacturer instructions and the resulting cDNA was used for capsid sequence recovery ...
-
bioRxiv - Plant Biology 2022Quote: ... The 10 μl reaction mixture contained 5 μl 2X SYBR Green/ROX master mix (Applied Biosystems), 2 μl of 1:20 diluted cDNA ...
-
bioRxiv - Systems Biology 2022Quote: ... Experimental starvation medium is DMEM/F12 medium supplemented with 5% (by volume) horse serum (Gibco #16050122), 0.5 mg/mL hydrocortisone (Sigma #H-0888) ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were washed with D-PBS and blocked with 5% horse serum (Thermo Fisher, #26050070), 1% BSA in D-PBS with 0.3% Triton X-100 for 1 h (RT) ...
-
bioRxiv - Neuroscience 2022Quote: ... QPCR was performed with Applied Biosystems QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific, MA, USA). Oligonucleotide primers are listed in Table S1 and primers that amplify Actb and Gapdh were used as reference genes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Primary GR antibody (G-5, Santacruz sc-393232, 1:100) or MAP2 antibody (Invitrogen, 1:2000) incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5 µg of total RNA per sample was treated with 2U of TURBO DNase (ThermoFisher Scientific) for 1 h.
-
bioRxiv - Microbiology 2023Quote: ... Presumptive macrococci colonies were streaked onto blood agar supplemented with 5% sheep blood (Oxoid, ThermoFisher, Johannesburg), incubated for 24 hours at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... Gc were pelleted and resuspended in 5 μg/mL goat anti-mouse AF488 (Invitrogen, Carlsbad, CA) diluted in RPMI + 10% FBS for 30 minutes at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
-
bioRxiv - Cell Biology 2024Quote: ... and reversed transfected at a concentration of 5 nmol/L using Lipofectamine RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: ... Cells were collected into fluorescence-activated sorting tubes and co-stained with 5 µM 7AAD (Invitrogen) and 1X BD Pharmingen™ PE Annexin V (BD Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... washed for 5 min with PBS and mounted with Permafluor (Thermo Fisher Cat# TA-006-FM). Primary mixed glial cultures were fixed for immunofluorescence staining at 5 ...
-
bioRxiv - Neuroscience 2022Quote: ... washed for 5 min with PBS and mounted with Permafluor (Thermo Fisher Cat# TA-006-FM). The following primary antibodies were used ...
-
bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked using either 5% milk or Superblock (PBS) Blocking Buffer (ThermoFisher, Cat#37515). Primary antibody was applied overnight at 4°C for PDE11A (Fabgennix PD11-101 at 1:500) ...
-
bioRxiv - Neuroscience 2022Quote: ... followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific; Cat#: 28904; Thermo Fisher Scientific; Cat#: A955)in a centrifuge at 3000 rpm ...
-
bioRxiv - Neuroscience 2022Quote: ... was dissolved in 5% DMSO and then diluted with saline solution (DPBS, 14190-144, Life Technologies) to reach 1 mg/mL CNO and 0.25% DMSO ...
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented either with 5 gm/L AlbuMax I (for 3D7, R539T and RKL-9; Gibco, USA) or 10% heat inactivated human A+ serum (for NF54 ...
-
bioRxiv - Molecular Biology 2022Quote: ... reagent in technical duplicates using the QuantStudio™ 5 Real-Time PCR cycler (Thermo Fisher Scientific) and following conditions ...
-
bioRxiv - Molecular Biology 2022Quote: ... qPCR was carried out on a QuantStudio 3 or QuantStudio 5 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
-
bioRxiv - Immunology 2022Quote: ... 4×104 sorted peritoneal and thymic macrophages were stained with 5 µM eFluor 450 (Thermo Fisher) in PBS for 10 min at 37℃ ...
-
bioRxiv - Neuroscience 2022Quote: ... VA) were cultured at 37 °C and 5% CO2 in DMEM-HG (Life Technologies, Carlsbad, CA) supplemented with 10% FBS (Clontech ...
-
bioRxiv - Biophysics 2022Quote: ... labeled for 30 min with 5 µM Alexa FluorTM 488 C5 maleimide (Thermo Fisher Scientific, A10254) in high potassium KD98 solution (98 mM KCl ...
-
bioRxiv - Bioengineering 2022Quote: ... RBC (5 million cells/mL) were incubated with 2 μg/mL calcein AM (Thermo Fisher Scientific) for 15 minutes at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... VOs we’re fixed for 1 hour in 4% paraformaldehyde solution (Thermo Fisher Scientific, 7732-18-5) in PBS and for 2 hours in 2.5% glutaraldehyde in PHEM buffer (TAAB ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged approximately every 3-5 days (70-85% confluency) using Accutase (Gibco, #11105-01) to detach cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 ug of empty vector or FLAG-POLR3B was transfected per well using lipofectamine 2000 (Invitrogen). After 24h ...
-
bioRxiv - Molecular Biology 2024Quote: Reverse siRNA transfections were done using 5 nM Silencer Select pre-designed siRNAs (Thermo Fisher Scientific) with Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: HEK293T cells were grown at 37°C in a 5% CO2 atmosphere in DMEM (Gibco 11995065), supplemented with 10% FBS ...
-
bioRxiv - Bioengineering 2024Quote: ... qRT-PCR was then performed using the QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific). ΔCt values were calculated using GAPDH and compared between treatment groups ...
-
bioRxiv - Bioengineering 2024Quote: ... IVT was then performed using MEGAscript® T7 Transcription Kit (Thermo Fisher Scientific, AMB-1334-5) according to the manufacturer’s instructions with modifications as using the CleanCap® Reagent AG (TriLink ...
-
bioRxiv - Microbiology 2024Quote: ... 5 μl of DNase I buffer (Fermentas) and 0.5 μl of Superase In (Thermo Fisher Scientific) were added to the denatured RNA and incubated at 37°C for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Nucleus were stained for 5 min with Hoechst n°33342 (ref H3570, Invitrogen, Carlsbad, California, USA) at 1:2000 ...
-
bioRxiv - Biochemistry 2024Quote: ... were all grown at 37°C in 5% CO2 using DMEM/F-12 medium (Gibco, #10565042) supplemented with 5% horse serum (Gibco ...
-
bioRxiv - Biophysics 2024Quote: ... The trapping column was PEPMAP NEO C18 (5 µm particle size, 300 µm*5mm, Thermo Scientific). Analytical column was nano EaseTM M/Z HSS C18 T3 (100Å ...
-
bioRxiv - Cell Biology 2024Quote: HL-60 cells were cultured with IMDM containing 20% FBS (Gemini) and 5% Penicillin-Streptomycin (Gibco) and maintained at 1 x 105 and 1 x 106 cells/ml ...