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Citations for Thermo Fisher :
7201 - 7250 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 5 μg of total RNA was further digested with DNase using a DNA-free kit (Ambion). The amount and purity of all RNA samples isolated were assessed by NanoDrop spectroscopy (Thermo Fisher) ...
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bioRxiv - Microbiology 2019Quote: ... Yale University) were maintained at 37°C/5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum (HIFBS ...
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bioRxiv - Immunology 2021Quote: PBMCs were activated for 5 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific). RNA was extracted from flow-sorted naive CD8+ T cells (n = 300 per condition ...
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bioRxiv - Immunology 2021Quote: ... cells were washed once with PBS and incubated with 5 μM CM-H2DCFDA (Life Technologies-Invitrogen), a general oxidative stress indicator ...
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bioRxiv - Immunology 2021Quote: ... cells were washed once with PBS and incubated with 5 μM CM-H2DCFDA (Life Technologies-Invitrogen), a general oxidative stress indicator ...
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bioRxiv - Immunology 2021Quote: ... Samples were run in technical triplicates using the QuantStudio 5 Real-Time PCR System (Thermo Fisher) with 10 min initial activation at 95°C followed by 40 cycles of 15 sec at 95°C ...
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bioRxiv - Immunology 2021Quote: ... thawed PBMCs were resuspended at 5 × 106 cells/well in AIM-V medium (Thermo Fisher Scientific) supplemented with Flt3 ligand (Flt3L ...
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bioRxiv - Bioengineering 2021Quote: ... The bioluminescence from each well was detected by a microplate reader (Fisher Scientific BioTek Cytation 5) with an exposure of 200 milliseconds.
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bioRxiv - Immunology 2020Quote: ... which were labeled with 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes, Eugene, OR) as described (52) ...
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bioRxiv - Immunology 2020Quote: All cells were cultured at 37 °C with 5 % CO2 in RPMI 1640 containing glutamine (Invitrogen) and supplemented with 10 % FBS (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... glass slides were functionalized with 5 μg/mL of Anti-CD3 (OKT3) (eBiosciences, Thermo Fisher Scientific) or 5 μg/mL of IgG or 100 μg/mL of PLL for 30 minutes at room temperature followed by extensive rinsing with PBS + 0.1% of BSA ...
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bioRxiv - Microbiology 2021Quote: ... 5% CO2 in HMI-9 medium supplemented with 20% heat inactivated Foetal μg/ml streptomycin (Gibco). Cell lines were µg/ml G418 (parental ...
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bioRxiv - Cell Biology 2021Quote: ... made of 5:1:1 ratio of H2O : H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: HeLa Kyoto cells were cultured at 37°C with 5% CO2 in MEM-α medium (Gibco) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... in a final reaction volume of 7.5µl on a QuantStudio 5 384 PCR machine (ThermoFisher Scientific). Samples were run in technical duplicate as two wells in the same qPCR plate and results were analysed using PBGD/RPLP0 as housekeeping genes ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples (5–20 µg) were separated with SDS-PAGE and transferred to PVDF membranes (Invitrogen). The membrane was blocked in Blocking-one (Nacalai ...
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bioRxiv - Genomics 2020Quote: ... and 30 µL from each sample was diluted 1:5 with serum-free MEM (ThermoFisher Scientific), and added to individual wells of a 12 well plate with confluent BF-2 monolayers ...
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bioRxiv - Cell Biology 2021Quote: ... 5% (w/v) glycerol (Applichem, A0970)) in the presence of 2.03 μM Hoechst 33342 (Invitrogen, H1399) when using unlabeled arrays for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were counted by mixing 5 µl of Trypan blue (Thermo Fisher Scientific, cat. no. T10282) with 5 µl of the sample and loaded on INCYTO C-Chip Disposable Hemocytometer ...
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bioRxiv - Genetics 2020Quote: ... tauschii was labelled with ChromaTide™ Alexa Fluor™ 594-5-dUTP (Invitrogen; C11400; coloured red) and 4 ...
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bioRxiv - Neuroscience 2020Quote: ... or primary antibody against Cld-5 (mouse monoclonal, C43C2, 35-2500, Thermo Fisher scientific, 1:200). AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody ...
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bioRxiv - Genetics 2020Quote: ... Antibodies were NKX2-5 (mouse, R&D, 1:500) and β -actin (rabbit, Invitrogen, 1:1000). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: 5×105 cells were transfected with 2ug of PBase using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Each tumor was minced with a clean blade and then dissociated in 5 mL RPMI (Gibco) containing 1 mg/mL collagenase IV (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... Pellets were diluted to a concentration of 5 × 104 cells/μL with ACK lysis buffer (Gibco). Samples were mixed by pipette and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 5 × 106–10 × 106 hybridoma cells using TRIzol reagent (Invitrogen, Gaithersburg, MD). Heavy (H)- and light (L)-chain cDNAs containing the gene encoding the antibody-binding (Fab ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 ml of 0.08% trypsin containing 0.35 mM EDTA (25200-072, Life Technologies, Somerset, NJ) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... the media was changed to 1:1 KSR: N2B media with puromycin (5 μg/ml, Gibco), where N2B was composed of DMEM/F12 ...
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bioRxiv - Immunology 2022Quote: ... absolute cell numbers were quantified using 5 µL of 123count eBeads Counting Beads (Thermo Fisher Scientific) added to each sample.
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bioRxiv - Cell Biology 2022Quote: ... were grown at 37°C with 5% (vol/vol) CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequent incubation at 37°C with 5% CO2 in a cell incubator (Thermo Fisher Scientific) for another 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... we embedded 5 μm fluorescent microspheres and a separate sample of 10 μm microspheres (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37°C with 5% CO2 in high glucose DMEM (Gibco, 11965-092) supplemented 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... qPCR experiments were performed using QuantStudio 5 Real-time PCR system (Thermo Fisher Scientific, MA, USA) and SYBR Green Master Mix (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... were obtained from American Type Culture Collection (ATCC) and cultured at 37°C and 5% CO2 in either RPMI-1640 (Gibco 11835-030) or Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biophysics 2022Quote: ... The RNA digestion was performed by addition of 5 U of RNase T1 (Thermo Fisher Scientific) and 5 μg of RNase A (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were sectioned at 5 μm using a rotary microtome (Thermo Fisher Microm HM310 Rotary Microtome) and affixed to clean slides.
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bioRxiv - Cancer Biology 2022Quote: ... TaqMan Assay reaction mixtures contained: 5 μL of TaqMan® Fast Advance (Applied Biosystems, Warrington, UK), 2.5 μL nuclease-free H2O ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed CsgF protein was then passed through a 5 mL Zeba spin desalting column (Thermo Fisher) that was equilibrated with 25 mM Tris pH 9 buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Image-iTTM Red Hypoxia Reagent (5 μM) and Cell Tracker DeepRed (0.1 μM) (all from Invitrogen). Cell samples were split into five equal volumes and subsequently encapsulated in 5.6 nL droplets in a continuous phase of fluorinated oil Novec HFE-7500 (3M ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at 37°C and 5% CO2 in DMEM/Glutamax (#31966; Gibco, Invitrogen Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at 37°C and 5% CO2 in DMEM/Glutamax (#31966; Gibco, Invitrogen Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at 37°C and 5% CO2 in DMEM/Glutamax (#31966; Gibco, Invitrogen Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: 200,000 cardiomyocytes were incubated in Amplex Red (5 μM; 2 min) for cellular ROS (A22177, ThermoFisher), or MitoSOX Red (5 μM ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Thermo Fisher Scientific; 11965092) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: We maintained all cells at 37°C and 5% CO2 in a Heracell incubator (Thermo Fisher). We cultured HEK293 cells in minimum essential medium (MEM ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-EU visualization used hapten-containing azide and Click-iT Cell Reaction Buffer Kit (Thermo Fisher). Samples were washed twice before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 1000 rpm for 5 min at 4°C (accuSpin Micro 17R; Fisher Scientific). The proteins were extracted in 50 mM Tris buffer (pH 7.8 ...