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Citations for Thermo Fisher :
7201 - 7250 of 7736 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... along with the N-terminal peptidase domain of human ACE2 was expressed using the Bac-to-Bac® baculovirus system (Invitrogen). The RBD (Residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2020Quote: ... cells were then washed 3 times with PBS and incubated with goat anti-rabbit Alexa Fluor 488 or goat anti-human Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific) for 1h at RT ...
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bioRxiv - Biophysics 2021Quote: ... Cultured cells were stained with mAb 0614 or human chimera MAb 0614-5 as the primary antibody and Alexa Fluor 488– or 647–conjugated anti-rat or human-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: The pCS2 Lrp6-APEX2-IRES-GFP-PAC plasmid was transfected into Human Embryonic Kidney 293T (HEK293T) cells using Lipofectamine2000 (Thermo Fisher Sci.). 48 hours after transfection puromycin was added to the culture medium ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin were cloned into human expression vectors driven by the CMV promoter (Table 3) and co-transfected into FreeStyle 293-F cells (Thermo Fisher Scientific). The empty 5Myc plasmid (CS2P ...
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bioRxiv - Immunology 2021Quote: ... and on the surface of H1568 EVs (with IFN-γ stimulation) were quantified using a PD-L1 Human ELISA kit (#BMS2212, ThermoFisher Scientific). For the cells ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA samples from affected individuals and unaffected family members were subjected to WES using Agilent SureSelect™ human exome capture arrays (Life Technologies) with next generation sequencing (NGS ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... 200,000 cells were transfected in 12 well plate with 25 pmol siRNA (siTFEB : ON-TARGETplus Human TFEB, L-009798-00-0005, Dharmacon™) using Lipofectamine RNAiMAX Transfection Reagent (1:200; Life Technologies). Cells were incubated 72 h at 37°C prior to further manipulation or drug treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were then incubated with Alexa 594 goat-anti rabbit secondary antibody at 1:750 and with Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT.
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bioRxiv - Molecular Biology 2020Quote: The dual pri-miRNA expression plasmids were transfected to human embryonic kidney (HEK) 293 cells in 6-well plates using the Lipofectamine 3000 reagents (Thermo Fisher Scientific) following standard procedures ...
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bioRxiv - Neuroscience 2021Quote: ... cRNA transcripts encoding human KCNQ1-5 were generated by in vitro transcription using the T7 polymerase mMessage mMachine kit (Thermo Fisher Scientific) or SP6 polymerase mMessage mMachine kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cultured in 10% human AB serum (produced in house) and 100 U/mL penicillin and 100 µg/mL streptomycin (Thermo Fisher Scientific) supplemented RPMI 1640 medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... we introduced the hAsCas12a gene into the human HEK 293 T-REx cell line nuclear genome (Flp-In ™ T-REx ™ −293 Cell Line, Invitrogen) allowing controlled expression by the tet-on system ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized primary human fibroblasts (gift from Paola Scaffidi) expressing human telomere reverse transcriptase (hTERT) (Scaffidi and Misteli, 2011) were grown in MEM (Thermofisher, 11095-080) supplemented with 15% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Media were collected after 24h in culture and cytokine/chemokine concentrations analyzed using the Inflammation 20-Plex Human ProcartaPlex™ panel (EPX200-12185-901, Thermo Fisher) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Immunology 2021Quote: ... or unseparated PBMCs (1 × 105 per well) were stimulated for 48-72 hours either with Dynabeads™ Human T Activator CD3/CD28 antibody coated beads (Thermo Fisher) at 2:1 or 4:1 bead:cells ratios or with plate-bound anti-CD3 and soluble anti-CD28 ...
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bioRxiv - Genomics 2019Quote: Plasmids were transfected into the human embryonic kidney 293 cell line (HEK293T; ATCC CRL-3216) and grown in DMEM media (Gibco, 10566-016), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with Aß-S8C dimer was subjected to hybridization in duplicates on the GeneChip PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Cell Biology 2019Quote: ... and human ELYS-IDR-C2 (aa. 2034–2275) were artificially synthesized with the codon usage optimized for bacterial expression (Thermo Fisher Scientific), and sub-cloned into pET28-a (+) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was isolated from the right middle lobe of mice and from pathologic samples from human lung tissue using Trizol (Invitrogen, Carlsbad, CA). Reverse transcription utilized the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... iPSCs were cultured on a feeder layer of irradiated mouse embryo fibroblasts using human embryonic stem cell media containing DMEM with F12 (DMEM/F12, 1:1 ratio, Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Bound cynomolgus monkey antibodies were detected with alkaline phosphate-labeled goat-anti-human IgG (1:1000, 4HI1305, Invitrogen Life Technologies, Bleiswijk, Netherlands) or alkaline phosphate-labeled goat-anti-human IgM (1:2000 ...
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bioRxiv - Immunology 2019Quote: ... A panel of 20 inflammatory cytokines were measured in duplicate by ProcartaPlex Human Inflammation Panel 20-plex (ThermoFisher Scientific, San Diego, USA) on a Luminex 200 System (Luminex Corporation ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C with 5% CO2 and transfected by different expression constructs for human IgG-Fc with various signal peptide coding regions using LipofectAmine (Invitrogen, Calsbad, CA) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... University of Southern California) were maintained in StemFit (amsbio) with 100 ng/ml human FGF2 (R&D) on Geltrex (Thermo Fisher Scientific) coated six well plates ...
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bioRxiv - Cancer Biology 2019Quote: ... T-cells were isolated 5 days prior to addition to MCF7 cells and were activated with Dynabeads® Human T-Activator CD3/CD28 (Thermofisher, 11131D) and 2.5 ng/mL of recombinant human IL-2 (Thermofisher ...
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bioRxiv - Neuroscience 2019Quote: Culture medium from 7 div OBSCs was assayed for human Aβ1-40 and Aβ1-42 using commercially available ELISA kits (Life Technologies: KHB3441 and KHB3481). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... gondii parasites were maintained by serial passage in confluent human foreskin fibroblast (HFFs, ATCC# SCRC-1041) monolayers in Dulbecco’s Modified Eagle’s Medium (DMEM, Life Technologies-Gibco, Cat# 10569-010), supplemented with 1% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: Human CXCL9 and CXCL10 cDNA flanked by XhoI and BamHI restriction sites were ordered as GeneArt™ Strings™ DNA Fragments (Invitrogen). DNA strings were digested with XhoI and BamHI in CutSmart buffer (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Neuroscience 2020Quote: ... Primary human dermal fibroblasts were collected from VWMD patients (2) or control family members (2) and maintained in DMEM/F12 (Life Technologies, 21331020), 10% FBS (Bovogen ...
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bioRxiv - Cell Biology 2020Quote: ... human S1R gene was amplified by PCR and cloned into pFastBac-HTA vector (Bac-to-Bac baculovirus expression system, Thermo Fisher Scientific) using EcoRI/HindIII sites ...
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bioRxiv - Cell Biology 2020Quote: ... staining (outside human-marked tissue) or by immunostaining with fluorescently-conjugated wheat germ agglutinin (WGA alexafluor-647, W32466, 1:500, Thermo Fisher Scientific). Specifically ...
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bioRxiv - Cell Biology 2021Quote: ... and human lung carcinoma cell line A549 (ATCC CCL-185) were cultured in the presence of exosome-depleted FBS (Thermo Fisher Scientific) prior to collection of conditioned medium ...
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bioRxiv - Bioengineering 2021Quote: ... A 2kb region surrounding the TRAC locus was amplified by PCR from human genomic DNA and cloned into a pCR blunt II TOPO backbone (Thermo Fisher Scientific). The CAR transgene from a pSFG.iCasp9.2A.14G2A-CD28-OX40-CD3ζ RV-CAR plasmid (gift from Dr ...
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bioRxiv - Biochemistry 2022Quote: ... Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were isolated from PBMCs using Pan T Cell Isolation Kit (Miltenyi, 130-096-535) and activated with Dynabeads® Human T-Expander CD3/CD28 (Gibco, 11141D). Three days later ...
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bioRxiv - Neuroscience 2020Quote: ... HCV-29 human derived urothelial cells were obtained as a gift from Paul Brindley and grown in MEM (Thermofisher Scientific, Waltham, MA) with 10% fetal bovine serum (Sigma-Aldrich ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human wild-type (WT) alpha-synuclein was expressed in Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific) cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human wild-type (WT) alpha-synuclein was expressed in Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific) cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: The DNA sequence of monomeric GFP was fused at the C-terminal of all human LI-cadherin constructs of which stable cell lines were established and was cloned in pcDNA™5/FRT vector (ThermoFisher Scientific). CHO cells stably expressing LI-cadherin-GFP were established using Flp-In™-CHO Cell Line following the manufacturer’s protocol (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were treated for 30 minutes with a 100 µL solution containing a mouse-anti-human ASGPR1 antibody (Thermofisher, Waltham, MA) diluted 1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... human induced pluripotent stem (iPS) cells cultured on Matrigel-coated plates were dissociated with warm enzyme-free dissociation buffer (Gibco, 13150-016) and centrifuged twice at 200xg for 5 min each in advanced DMEM/F12 (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... episomally derived from human foreskin fibroblasts) was cultured on tissue culture-treated plasticware pre-coated with 1mL per well Geltrex (Life Technologies A1413302) for 1 hour at 37C ...