Labshake search
Citations for Thermo Fisher :
7151 - 7200 of 7736 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher), as per manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Previously homogenized human-derived AD crude brain extracts were diluted 1 to 20 with Opti-MEM (Thermo Fisher Scientific, cat. 31985062) and sonicated in a Qsonica multiplate horn water bath for 3 minutes at 40% power ...
-
bioRxiv - Immunology 2023Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: The human alveolar epithelial cell line A549 (ATCC CCL-185) was cultured in F-12K culture medium (Gibco™ 21127-030) supplemented with 10% fetal calf serum (FCS ...
-
bioRxiv - Cancer Biology 2023Quote: ... human prostate tissues were first digested in aDMEM/F12/Collagenase II (1.5mg/ml)/Hyaluronidase VIII (1000 u/ml; Thermo Fisher Scientific) plus 10 μM Y-27632 (Tocris ...
-
bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human liver homogenate was prepared from human FLC or matched normal liver tissue (adjacent to the FLC) using RIPA Lysis buffer (Thermo Fisher) with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher) ...
-
bioRxiv - Pathology 2023Quote: ... 25 nM) or Human YAP/TAZ-siRNA (UGUGGAUGAGAUGGAUACA) (102) by using Lipofectamine RNAiMAX reagent (13778075, Thermo Fisher Scientific, Waltham, MA, USA). Cells were harvested after 72 h.
-
bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8; ATCC CCL-244) were cultured in RPMI 1640 ATCC Modification medium (Thermo Fisher A14091-01) supplemented with 10% fetal bovine serum (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... The experiments were normalized using human β-Actin as the endogenous control (FAM/MGB probe, cat. no. 401846; Applied Biosystems, USA).
-
bioRxiv - Bioengineering 2023Quote: Human kidney epithelial cell line HEK293T was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
-
bioRxiv - Bioengineering 2023Quote: Human lung Carcinoma cell line A549 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units /ml Pen-Strep (GIBCO ...
-
bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and dilutions were used: Alexa Fluor 568- and 647-conjugated goat anti-human and anti-rabbit (Life Technologies), 1:1000 ...
-
bioRxiv - Bioengineering 2023Quote: Human lung Carcinoma cell line A549 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units /ml Pen-Strep (GIBCO ...
-
bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
-
bioRxiv - Molecular Biology 2023Quote: The surface of all cultureware used for the maintenance and propagation of human pluripotent stem cells (WTC-11 line) was coated with Corning Matrigel (Fisher Scientific) prior to cell seeding ...
-
bioRxiv - Genetics 2023Quote: ... 293T human embryonic kidney epithelial cells (ATCC, #CRL-3216) were grown in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher, #11995-065) supplemented with 10% FBS ...
-
bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... we used quantitative TaqMan based OpenArray Human MicroRNA Panel (Cat# 4470187; Thermo Fisher Scientific, Life Technologies Corporation, Grand Island, New York). This panel contains 754 well-characterized human miRNA sequences from the Sanger miRBase v14 ...
-
bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
-
bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
-
bioRxiv - Systems Biology 2023Quote: ... IgG1 and IgG4 in the basolateral media were quantified using human ELISA) IgG1 and IgG4 quantification kits (Invitrogen, EHIGG1 and BMS2095) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
-
bioRxiv - Immunology 2023Quote: Polyclonal T cells were isolated from PBMCs (see above) and pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron) ...
-
bioRxiv - Developmental Biology 2023Quote: 100 ng total RNA was reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression Kit following the manufacturer’s protocol (Thermo Fisher Scientific). Target genes (SQSTM1 ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
-
bioRxiv - Cell Biology 2023Quote: MRC5 fibroblasts (ATCC cat. no. CCL-171) and adult human mesenchymal cells were cultured in DMEM (cat. no. 11965092, Thermo Fisher) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
-
bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
-
bioRxiv - Immunology 2023Quote: ... and processed for quantitative PCR analysis of SMAD3 and SP1 binding to the human MCEMP1 promoter using Power SYBR Green PCR Master Mix (ThermoFisher Scientific). ChIP-qPCR results were calculated using the ΔΔCt method and are shown as percentage of input DNA for each ChIP experiment.
-
bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA was isolated from tissue lysates as well as from cultured human and murine aortic or DA SMCs with the PureLink RNA Mini Kit (ThermoFisher, 12183018). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: Cell line derived from human neuroblastoma (SH-EP; CVCL_RR78) was verified by STR profiling and grown in RPMI-1640 (Thermo Fisher Scientific). Murine neuroblastoma cells (NHO2A ...
-
bioRxiv - Immunology 2024Quote: ... tissue samples with small volumes (such as human samples) were then included in 1.5% agarose (Invitrogen, Carlsbad, CA, USA; #16500-500). Tissue clearing was then carried out with MetOH dehydration (as described above ...
-
bioRxiv - Biochemistry 2024Quote: Human liver specimens were minced to extract genomic DNAs (gDNAs) using the PureLink™ Genomic DNA Mini Kit (Invitrogen™, K182001), and genomic DNA concentrations were measured using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Scientific™ ...
-
bioRxiv - Bioengineering 2024Quote: ... Human dermal fibroblasts (ZenBio, Cat. # DF-F) were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Cat. # SH30021FS) with 10% exosome-depleted FBS (Bio-Techne ...
-
bioRxiv - Cell Biology 2024Quote: IMR-90 human fibroblast was purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen ...
-
bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
-
bioRxiv - Immunology 2024Quote: ... on semiconfluent monolayers of OP9 stromal cells expressing the human Jag2 Notch ligand (OP9-Jag2) (60) and cultured in p24-well plates with α-MEM medium (Gibco) supplemented with 20% FBS ...
-
bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
-
bioRxiv - Microbiology 2024Quote: MRC-5 human embryonic lung fibroblasts were purchased from ATCC (Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco Thermo Fisher) supplemented with 10% FBS (Gibco Thermo Fisher) ...
-
bioRxiv - Neuroscience 2024Quote: ... Additionally, human embryonic kidney HEK293T/17 cells (ATCC, Manassas, VA, USA, Cat# CRL-11268) were grown in DMEM media (Thermo Fisher Scientific ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: The human neuroblastoma cell line (SH-SY5Y: ATCC, CRL-2266) was grown in low glucose DMEM medium (11885084, Thermo Fisher Scientific) with 10% fetal bovine serum (Sigma-Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
-
bioRxiv - Cancer Biology 2024Quote: RNA isolation and RT-qPCR for human tissue were performed as described previously (O’Donnell et al., 2023) using a hybrid TRIzol (Life Technologies 15596018) and RNeasy Plus Mini kit protocol after liquid nitrogen pulverization ...
-
bioRxiv - Immunology 2024Quote: ... and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions ...
-
bioRxiv - Immunology 2024Quote: ... and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions ...