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Citations for Thermo Fisher :
7001 - 7050 of 7736 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Invitrogen #A18805), goat anti-human IgM (Invitrogen #A18835) ...
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bioRxiv - Biochemistry 2019Quote: ... pcDNA3.1P-gp was constructed by the insertion of cDNA encoding human P-gp in pcDNA3.1 (Thermo Fisher Scientific, Austin, TX, USA). To engineer P-gp–Venus ...
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bioRxiv - Genetics 2021Quote: ... into the melanoma cell line UACC 903 and multiple human melanocyte cultures (c23, c197 and c87) using Lipofectamine 2000 reagent (Thermo Fisher) for UACC903 or electroporation with the Lonza Amaxa P2 kit and the CA-137 protocol on the 4D-Nucleofector system for primary melanocytes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Both of the in vitro systems depend on the application of three-dimensional embryoid body cultures derived from either mouse embryonic stem cells (mESC, ES-D3, ATCC, CRL-1934) or human episomal induced pluripotent stem cells (hiPSC, Gibco, A18945). The teratogenicity determination of the mEST is based on the half maximal inhibition of differentiation into beating cardiomyocytes (ID50 ...
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bioRxiv - Immunology 2020Quote: Soluble extracellular domain (ECD) of human CD58 (UniProt residues 29–204 or 29–213) was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent ...
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bioRxiv - Physiology 2020Quote: ... The cells were transiently transfected for 16-24 hours with either human or mouse orthologs of TRPA1 using Fugene 6 transfection reagents and Opti-MEM (Thermofisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Soluble extracellular domain (ECD) of human CD58 (UniProt residues 29–204 or 29–213) was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent ...
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... The human laryngeal cancer cell line (HEp2) was purchased from ATCC (CCL-23) and cultured in DMEM/F12 medium (Gibco, 11320033) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... chamber slides were incubated with secondary antibody Alexa-Fluor 488 goat anti-human IgG (H+L) (Invitrogen #A-11013, Paisley, UK) diluted 1:500 and Hoechst 33342 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... ATCC) and human kidney COS-7 cells (CRL-1651, ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco 11995-065) supplemented with 10% v/v heat-inactivated fetal bovine serum (Gibco 12483-020 ...
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bioRxiv - Bioengineering 2021Quote: DiPS 1016 SevA hiPSC line derived from human skin fibroblasts were seeded and kept in culture on Geltrex (1% Invitrogen, USA)-coated culture flasks using mTeSR (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Cell Biology 2020Quote: C2C12 myoblasts were transfected with a plasmid containing the N-terminal 88 amino acids of human cyclin B1 fused to EGFP using Lipofectamine 3000 (Invitrogen, L3000001) with the P3000 reagent according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and labeled on ice with 1:250 dilution of secondary antibodies for binding (anti-mouse 488, ThermoFisher A11059 or anti-human 647, ThermoFisher A21445) and expression (anti-chicken 647 ...
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bioRxiv - Biochemistry 2020Quote: ... and human CMP-N-acetylneuraminate-poly-α-2,8-sialyltransferase (ST8SIA4) (NP_005659)were codon-optimized for human expression and synthesized (Geneart, Thermo Fisher Scientific). A cDNA encoding a soluble version of human NCAM ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
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bioRxiv - Immunology 2020Quote: ... by negative selection magnetic bead sorting (Miltenyi) and activated in complete RPMI supplemented with 30 U/ml IL-2 with anti-human CD3/CD28 dynabeads (Thermo Fisher) at a 1:1 cell-to-bead ratio.
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bioRxiv - Molecular Biology 2019Quote: ... After 24h the cell suspensions were transferred into a new flask and PBMCs were stimulated by adding Dynabeads Human T-Activator CD3/CD28 (Life Technologies) in a 1:1 cell-to-bead ratio ...
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bioRxiv - Genetics 2019Quote: ... Read alignment to the human genome reference (hg19/GRCh37) and variant calling were performed with the Ion Torrent Suite Software v5.02 (Thermo Fisher Scientific).
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bioRxiv - Physiology 2019Quote: ... Approximately 20-40 mg of human ventricular myocardium was homogenized in 1 mL of Trizol (Life Technologies #15596-026, Carlsbad, CA) with a TissueLyser LT (Qiagen N.V ...
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bioRxiv - Genetics 2020Quote: ... Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies) followed by Lithium Chloride precipitation and quantitation using a NanoDrop (Thermo Fisher Scientific)
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bioRxiv - Genetics 2020Quote: ... hybridization mixtures were created from 5ul of the nick translated fluorescent RNA probe mixed with 10ul of human Cot-1 (ThermoFisher #15279011) and 2ul of Salmon Sperm DNA (ThermoFisher #15632011) ...
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bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 2×106 cells were cultivated for 24 h in presence of either Human T-Activator CD3/CD28 dynabeads (Thermo Fisher Scientific), according to manufacture’s instruction ...
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bioRxiv - Physiology 2021Quote: Human embryonic kidney 293T (HEK293T; ATCC, Manassas, VA, USA) cells were cultured in Opti-MEM I media (Invitrogen, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... Lenti-X Human Embryonic Kidney (HEK) cells were transfected with the sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Cat. No. L3000150), then cultured for 2-3 days in the following media ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue culture flasks until they reached 70-80% confluence in human ectocervical primary cell medium (Advanced DMEM/F-12 (Invitrogen, 12634) supplemented with 12 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant poly-histidine tagged ectodomains of the SARS-CoV-2 S proteins (614D and 614G) were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using HisTrap FF column (GE Life Sciences)18 ...
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bioRxiv - Immunology 2021Quote: ... Plates were slapped dry and washed 4x with TBST using a BioTek plate washer followed by addition of Invitrogen anti-Human IgG (ThermoFisher A18817) and one hour incubation at 37C ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Cell Biology 2020Quote: Human pluripotent stem cells were cultured and maintained on feeder-free plates with StemFlex Medium (Cat. No. A3349401, Thermo Fisher Scientific) as described 58 ...
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bioRxiv - Genetics 2021Quote: Human-derived cell line THP1 (ATCC TIB-202) was cultured in RPMI medium with 10% fetal bovine serum (FBS, GIBCO, USA). THP1 cells were activated with 100 ng/ml phorbo-12-myristate-13-acetate (PMA ...
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bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Cell Biology 2019Quote: MCF10A human mammary epithelial cells were a kind gift from Sabrina Spencer and were cultured in growth medium DMEM:F12 (Gibco, #11320-033) containing 5% horse serum (Gibco ...
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bioRxiv - Cell Biology 2021Quote: WT or ATG13-/- MCF10A cells (human breast epithelial), expressing GFP-LC3A (human), were prepared as described previously (Jacquin et al., 2017) and cultured in DMEM/F12 (Gibco, 11320074) containing 5% horse serum (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... or 1×105 (96-well plate) naïve CD8+ T cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 bead to T cell ratio and cultured in 1 ml (48-well plate ...
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bioRxiv - Cancer Biology 2021Quote: ... before incubation with the DAPI and secondary goat anti-human IgG fluorescently labeled with Alexa Fluor 488 (A-11013, ThermoFisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Wells were then washed four times in 1xDPBS followed by incubation with goat anti-human IgG Alexa Fluor 594 (Thermo Fisher) at a dilution of 1:200 in 1xDPBS for 30 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... human hepatocellular carcinoma) and HRT-18G (ATCC CRL-11663; human colorectal adenocarcinoma) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco Life Technologies) supplemented with 8% heat-inactivated fetal bovine serum (HyClone ...
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bioRxiv - Microbiology 2020Quote: ... murine alveolar macrophages MH-S (ATCC CRL-2019) and human monocytes THP-1 (ATCC TIB-202) were grown in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco 21875) supplemented with 10% heat-inactivated fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and ME49 hxgprt-ko ku80-ko (ME49) were grown in confluent human foreskin fibroblasts (HFF, ATCC SCRC-1041) in DMEM (Gibco 41965039) supplemented with 5% fetal calf serum and 40 μg/ml gentamycin (Gibco 15710-049) ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Microbiology 2021Quote: ... The human leukemia monocytic cell line THP-1 16 was cultured in Milieu Roswell Park Memorial Institute (RPMI) medium (ThermoFisher Scientific) enriched with 10% fetal calf serum (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: Human iPSC-derived microglia (Tempo-iMG™; Tempo Bioscience, CA) was cultured in DMEM/F-12 (Thermo Fisher, Waltham, MA, USA) supplemented with 1X N2 supplement ...
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bioRxiv - Immunology 2020Quote: ... Secreted factors were measured in the T cell assay culture media using a Luminex kit (Cytokine Human Magnetic 30 Plex Panel, Thermo Scientific) per manufacturer’s recommendations with 4 replicates per sample ...