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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 1×106 cells were electroporated with 0.5 μg of plasmid DNA in a final volume of 200μl of Opti-MEM I (31985062; Gibco), in a 2 mm electroporation cuvette (Z706086 ...
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bioRxiv - Biochemistry 2022Quote: ... PAC KO cells were transfected with 500–800 ng/ml of plasmid DNA using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A total of 36 μg VP1 plasmid DNA was mixed with 1.5 mL of Opti-MEM I (ThermoFisher). 72 μL of Lipofectamine 2000 was diluted in 1.5 mL of Opti-MEM I and incubated for 5 min at room temperature prior to mixing with the diluted plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transiently transfected into HEK293T or MCF-7 cells at 70% confluence using Lipofectamine 3000 (Invitrogen, #L3000015) in a 4:1 v/w ratio following manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... All cells were reverse transfected with 500 ng minigene plasmid DNA with Lipofectamine 3000 and P3000 reagent (Invitrogen) in 12 well plates ...
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bioRxiv - Genetics 2019Quote: ... 1 µg of each sgRNA plasmid were transfected in C57BL/6N C2 ESCs75 by lipofection (Lipofectamine 2000, ThermoFisher). After 16 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The complex of the transfection reagent and plasmids were prepared in opti-MEM reduced serum medium (Thermofisher, 31985070). Transfected cells were allowed to express the fluorescent proteins for the 24h before any experiment.
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bioRxiv - Genetics 2019Quote: ... The HL+ZEO+HR insert was ligated into the unique SacI site in plasmid pUC57 (Thermo Fisher Scientific) and transformed into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 1 μg linearized plasmid was used as template in SP6 mMessage mMachine in vitro transcription reaction (Ambion, AM1344) according to product instructions.
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bioRxiv - Neuroscience 2019Quote: ... they were transfected with plasmid expression constructs in 6-well tissue culture plates (Thermo Fisher Scientific, Burlington, ON) using Lipofectamine 3000 transfection reagent (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... mammalian CHO K1 cells stably overexpressing apo-aequorin and human Gα16 were transiently transfected with the nmur-1/pcDNA3.1 or empty pcDNA3.1 (control) plasmid using Lipofectamine LTX and Plus reagent (Invitrogen). After transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293T cells were cotransfected with pEGFP-Aly/Ref and pmCherry-NXF1 plasmids with lipofectamine 2000 (Thermo Fisher Scientific). Following the transfection ...
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bioRxiv - Microbiology 2020Quote: Each well of 293T cells in a 96-well plate was transfected with 0.1µg of ACE2 plasmid DNA following the standard protocol of Lipofectamine 2000 (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... The pMLM3613 plasmid encoding cas9 was used for in vitro transcription using the SP6 mMessage mMachine Kit (Ambion) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... MiRNA mimics and inhibitors as well as DNA plasmids were transfected using transfection reagent Lipofectamine 3000 (Invitrogen, USA) following the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2020Quote: ... cells were grown until they reached 60% confluency and the plasmids were transfected using Lipofectamine-3000 (ThermoFisher scientific) transfection reagents according to the manufacturer’s instructions.
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A LRRK2/dLRRK-mediated lysosomal pathway that contributes to glial cell death and DA neuron survivalbioRxiv - Neuroscience 2021Quote: ... 500 ng plasmid and 2 ul Lipo 2000 were diluted with 100 μl Opti-MEM (Cat. #31985070, Gibco), mixed ...
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bioRxiv - Microbiology 2020Quote: ... cells were co-transfected in triplicate with 0.4 μg of total DNA (0.2 μg of each plasmid) using Lipofectamine 2000 (Invitrogen). 24 h post transfection cells were washed with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection was performed using the JetPEI (Polyplus transfection, 101-40N) or Lipofectamine 2000 kits (Life technologies, 11668019) as recommended by the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with individual overexpression plasmids (2.4 μg DNA for a 3.5cm dish) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... sequenced using the T3 and T7 plasmid primers and analyzed using an ABI 310 Genetic Analyzer (Applied Biosystems). The obtained sequences were examined through BLAST analysis.
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bioRxiv - Pathology 2021Quote: ... and the Renilla luciferase-expressing plasmid pRL-TK were co-transfected into cells by using lipofectamine 2000 (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... 1984) and the SCR promoter were cloned into pDONR plasmids and then inserted into the binary pB7M24GW (Invitrogen) plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with 6 μg plasmid DNA per 106 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V ...
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bioRxiv - Immunology 2021Quote: ... using plasmid specific primers on StepOne™ Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific, Lithuana). Plasmid DNA at different concentrations (1 x 102 to 1 x 107 copies/mL ...
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bioRxiv - Immunology 2021Quote: ... using plasmid specific primers on StepOne™ Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific, Lithuana). Plasmid DNA at different concentrations (1 x 102 to 1 x 107 copies/mL ...
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bioRxiv - Genetics 2019Quote: ... the plasmid and the downstream SapI-containing PCR was digested with SapI (also called LguI from ThermoFisher, ER1931), phosphatase treated (Antarctic phosphatase ...
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bioRxiv - Cell Biology 2020Quote: ... 293T cells were transfected with pLenti-CMV-eGFP plasmid together with a lentviral packaging mix (Life Technologies, USA) to produce GFP lentivirus ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmid extract kits and T4 DNA ligase were the products of Fermentas(Thermo Fisher Scientific, Waltham, MA USA). DNA recovery kits were purchased from Biomed (Los Angeles Biomedical Research Institute ...
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bioRxiv - Biochemistry 2019Quote: ... or mismatch plasmids (1.5 μg per well in 6-well plate) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: mRNAs encoding Gaussia luciferase were generated on template of pJET_T7_Gluc_128A plasmid digested with restriction enzyme AarI (ThermoFisher Scientifics). The plasmid was obtained by cloning the T7 promoter sequence and coding sequence of Gaussia luciferase into pJET_luc_128A (34) ...
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bioRxiv - Neuroscience 2019Quote: The plasmid containing a codon-optimized neurotactin cDNA with 2xHA tag was obtained from GeneArt (ThermoFisher Scientific, USA). C ...
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bioRxiv - Cancer Biology 2019Quote: ... F0 Casper larvae were obtained by injecting 3 nL of 12.5 ng/mL DNA plasmid and 17.5 ng/mL in vitro transcribed (Ambion) transposase mRNA into the cell of one-cell stage embryo.
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bioRxiv - Genetics 2020Quote: ... On the day of transfection 0.5ug of combined plasmid DNA was mixed with 1.5ul of the Lipofectamine 3000 reagent as directed by ThermoFisher’s protocol and the whole mixture was pipetted into a well of a 24 well plate ...
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bioRxiv - Genetics 2020Quote: ... Cells were transfected 24 hours after seeding with 500 ng dual luciferase reporter plasmid using Lipofectamine3000 (Invitrogen L3000015) per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... cells were co-transfected in triplicate with 0.4 μg of total DNA (0.2 μg of each plasmid) using Lipofectamine 2000 (Invitrogen). 24 h post transfection cells were washed with PBS ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids for mammalian cell transfection were generated using Gateway cloning technology as previously described (Invitrogen, Carlsbad, CA, USA) (Katzen ...
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid concentrations were determined using the Qubit dsDNA broad range assay kit with a Qubit 3 fluorometer (Invitrogen). In vitro myTXTL reactions were prepared by combining 5 nM of pSLP15 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Linearized plasmids were used as DNA templates for cRNA synthesis using the mMessage mMachine T7 transcription kit (Ambion). cRNAs were precipitated with lithium chloride and were resuspended in a suitable volume of RNAse-free water and stored at −20°C before use.
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bioRxiv - Biochemistry 2021Quote: ... The plasmid pPICZ used to obtain the yeast α-factor secretion signal sequence and was obtained from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 100 ng of plasmid was transformed into DH10Bac competent cells (MAX Efficiency™ DH10Bac Competent Cells, Gibco #10361012) for bacmid DNA production ...
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bioRxiv - Immunology 2021Quote: ... Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen). The following day 300μL of ExpiFectamine Enhancer 1 and 3mL of Enhancer 2 (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: All vectors used for assembly and transfections were purified with GeneJET plasmid miniprep kit (Thermo Fisher Scientific, K0503) or GenEluteTM Plasmid MiniPrep Kit (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... was generated in-house to yield a plasmid named Httex197QTC1-Em Y66L in the pT-Rex vector (Invitrogen). The pT-Rex Em Y66L construct alone was also generated in-house as described previously (13) ...
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bioRxiv - Synthetic Biology 2022Quote: ... we first tried magnetic beads-based method using the MagJET plasmid DNA Kit (K2792) (ThermoFisher Scientific, Waltham, MA) on the Fluent ...
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... The cells were transiently transfected with individual GPCR-Tango plasmid using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000-015). After 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid transfer vector containing the TMPRSS2 gene was transformed into Escherichia coli DH10Bac cells (Thermo Fisher; Cat# 10361012) to generate recombinant viral bacmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... and transfected with 0.14 pmol of plasmid DNA using 2.5 μl per well of Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were transfected at about 70% confluency with plasmid DNA containing the RCAS constructs using Lipofectamine 3000 (Invitrogen). Transfected cells were grown for four days to enable viral replication ...