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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... from which it was cloned by Gateway LR reaction to pDEST-FTF/FRT/TO47 or pDEST53 plasmid (Invitrogen) for mammalian expression.
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bioRxiv - Cell Biology 2023Quote: ... from which it was cloned by Gateway LR reaction to pDEST-FTF/FRT/TO47 or pDEST53 plasmid (Invitrogen) for mammalian expression ...
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bioRxiv - Cancer Biology 2023Quote: KH2 ESC 9 were electroporated with pBS31-BCR-ABLp210 and FLP recombinase plasmids (NEON transfection system, Thermo Fisher) and selected with Hygromycin (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #3 was transcribed using the mMESSAGE mMACHINE T7 Transcription Kit (Thermo Fisher Scientific, AM1344) and purified with the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... pRL-CMV-Renilla (5 ng) and pReceiver-M12-SH2B3 (100 ng) plasmids using Lipofectamine 2000 (Invitrogen; cat # 11668019) resuspended in Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... cells were selected for presence of the gRNA plasmid using complete media plus 2.5µg/mL puromycin (Gibco, #A1113803) and 2.5µg/mL blasticidin ...
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bioRxiv - Immunology 2023Quote: Antibody heavy and light plasmids were co-transfected at a 1:1 ratio into HEK-293F cells (Thermofisher) using PEI Max (1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... Clonal genes were delivered as purified plasmid DNA ready for transient transfection in HEK Expi293 cells (Thermo Scientific). Cultures in a volume of 1.2 ml were grown for four days ...
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bioRxiv - Bioengineering 2023Quote: ... coli overnight with the appropriate selective antibiotic and then purifying using the PureLink Plasmid Midiprep Kit (Thermo Fisher), ZymoPURE II Midiprep Kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: ... HT29 cells were seeded in a 6-well plate upto 50-60% confluency and transfected with 2.5μg of cloned PGL4.12 and 50ng of PGL4 (hRluc-CMV) plasmid using lipofectamine-3000 as transfection reagent (Invitrogen). The transfected cells with cloned pGL4.12 and pGL4 (hRluc-CMV ...
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bioRxiv - Developmental Biology 2023Quote: ... R26M2rtTA and Oct4-GFP alleles were transfected with pCX-Gata4-E2A-E2-Crimson plasmid using Lipofectamine 3000 (Invitrogen). Oct4-GFP+/PDGFRα+ and Oct4-GFP-/ PDGFRα+ cells were flow sorted on Day 5 or Day 7 of conversion ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting plasmids were transfected into the One Shot OmniMAX 2-T1R Chemically Competent cells (Invitrogen, C8540-03) by heat shock ...
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bioRxiv - Cell Biology 2022Quote: ... Thereafter, the inserts were transferred to plasmid pMTN1G (Toya et al., 2010) by the LR reaction (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... This was achieved by combining a total of ∼1.0 μg of plasmid DNAs with serum-free OptiMEM (Gibco 31985062 ...
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bioRxiv - Immunology 2023Quote: ... 2.5×105 B16 cells were plated and transfected with 2μg of Cas9-and sgRNA-encoding plasmid DNA using Lipofectamine 3,000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed with ∼2.6 µg plasmid in 2 mL media with 10 μL Lipofectamine 2000 (ThermoFisher scientific). Complexes were allowed to form for 10 minutes and then added dropwise to the cells.
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bioRxiv - Microbiology 2023Quote: ... Overexpression from pSCrhaB2 plasmids transformed into Eco was induced by addition of 0.1% rhamnose (w/v) (Thermo Scientific) into liquid media ...
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bioRxiv - Biophysics 2023Quote: ... coli DH10EmBacY31 cells were transformed with previously cloned pFastBac1 plasmids according to the Bac-to-Bac manual (Invitrogen). The target gene replaced the viral polyhedrin gene using its promoter for high yield expression ...
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bioRxiv - Bioengineering 2023Quote: ... AsCpf1-Ultra mRNA was transcribed from the cloned plasmid using an mMESSAGE mMACHINE™ SP6 Transcription Kit (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... The NPM1-GFP cDNA was cloned into pInducer20 (Plasmid #44012) utilizing the Gateway Cloning technology from Thermo Fisher, with primer details provided in the primer list.
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bioRxiv - Cell Biology 2023Quote: ... pGEX-4T1-based plasmids harboring the construct of interest were transformed into BL21 Star(DE3)pLysS (ThermoFisher, C602003) and plated onto Ampicillin-containing LB plates ...
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bioRxiv - Biochemistry 2023Quote: The plasmid for the catalytic domain of NopD was cloned from pMx-NopD (purchased from Thermo Fisher Scientific). All constructs of pET28a-NopD were amplified by PCR and ligated using the “Restriction Enzyme Free PCR” methodology 39 ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected with dual gRNA CRISPR/Cas9-plasmid using Lipofectamine LTX with Plus reagent (Thermo Fisher Scientific, 15338100) and 0% serum EMEM media (QBI-112-018-101 ...
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bioRxiv - Genetics 2023Quote: All plasmids were created with the Multisite Gateway system with LR Clonase II Plus (Cat#12538120; Life Technologies) according to the manufacturer’s instructions and original Tol2 Kit information2,31 ...
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bioRxiv - Immunology 2023Quote: ... where indicated along with 6 μg pCL-Eco plasmid DNA using 60 μl Lipofectamine 2000 in OptiMEM (Invitrogen) for 8 h in antibiotic-free medium ...
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bioRxiv - Microbiology 2023Quote: ... and pYK-JRCSF HIV-1 expression plasmid in a 5:1 mass ratio with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s manual.
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bioRxiv - Immunology 2023Quote: ... plasmids were transfected into 293 T cell or Hela cell using Lipofectamine™ 3000 Transfection Reagent (Life Technologies) according to the manufacturer’s instructions ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Plasmid-free cultures were then centrifuged for ten minutes at 3,600 rpm (Thermo Scientific Megafuge 40R TX-1000), resuspended in 28 ml PBS ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid sequences were confirmed by Sanger sequencing with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (ThermoFisher Scientific), purified by the BigDye XTerminator™ Purification Kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... was used to linearize the plasmid vector for in-vitro transcription by MEGAscript SP6 transcription kit (Invitrogen # AM1330). Then ...
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bioRxiv - Cell Biology 2023Quote: ... and separately 14.5 µg CRISPR/Cas9 plasmid containing the cloned protospacer with 500 µl Opti-MEMTM (Thermo Scientific). The final transfection solution was obtained by mixing the prepared solutions ...
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bioRxiv - Biophysics 2023Quote: ... The coding sequence in the resultant plasmid was subcloned into the pFastBac™ Dual Expression Vector (Thermo Fisher) using EcoRI and HindIII sites (underlined in the primer sequences) ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... 24 μg plasmids and 24 μL FectoPro transfection reagent were mixed in 3 mL Opti-MEM medium (ThermoFisher), then incubated for 20 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... 1μg plasmid DNA was then digested in 50μL volume for 1hr with FD BstXI (Thermo Fisher Cat. #FD1024). Digested plasmid DNA was then Klenow-extended using added UMI linker (sequence ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected with the expression plasmid using Lipofectamine LTX Reagent with PLUS Reagent (Thermo Fisher Scientific, no. 15338100) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the assembled plasmids and empty vector controls were cleaned and concentrated via ethanol precipitation with yeast tRNA (ThermoFisher) as a co-precipitate (12 µg yeast tRNA per 300 ng assembled DNA per 840 uL precipitation) ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells in a T175 flask were transfected with 46.4µg DNA consisting of equal amounts of four plasmids using 138µL lipofectamine 2000 (Thermo Fisher). The plasmids were ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting plasmids were used in an LR reaction (Gateway LR Clonase II Enzyme Mix, # 11791-020 ThermoFisher) with plasmids pCFJ210 (#30538 Addgene) ...
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bioRxiv - Neuroscience 2024Quote: ... we transfected the DNA of one BC plasmid into each of the HEK samples using lipofectamine 2000 (Invitrogen) according to the manual ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid standards containing a copy of the Escherichia coli 16S rRNA gene were synthesized by GeneArt (ThermoFisher Scientific). Prior to qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... then transfected with TOP-Rluc or TOPmut-Rluc and Firefly Luciferase plasmids using Lipofectamine-2000 (Invitrogen, Cat #11668027) following recommended protocols ...
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bioRxiv - Biophysics 2024Quote: Plasmids with and without parS1 site (pUC57, supplementary material S1) were transformed into TOP10 cells (Invitrogen, cat C404003), grown in LB media with appropriate antibiotics ...
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bioRxiv - Cell Biology 2024Quote: ... and CCSer2-SxNN1,4 U2OS cells were transfected with 0.5 µg of plasmid using Lipofectamine LTX reagent (Thermo Fisher) as per manufacturers instruction ...
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bioRxiv - Biochemistry 2024Quote: ... of either HEK293 or HCT116 p300- in the respective media was seeded in a T-25 flask and 24 hours later was transfected with EP300 plasmid construct (1 µg or 2.5 µg) using Lipofectamine 3000 according to the manufacturer’s protocol (Invitrogen). Following 24 hours of transfection ...
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bioRxiv - Microbiology 2024Quote: Clinical isolates with emrR or oqxR mutations were transformed with pTOPO expression plasmids (pCR-Blunt II-TOPO, Invitrogen) harboring the wild-type gene (pTOPO_emrR or pTOPO_oqxR ...
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bioRxiv - Microbiology 2024Quote: ... Stable transfectants carrying plasmids with BSD-selectable marker were initially selected on 2 μg/ml blasticidin-S (Invitrogen). In order to obtain parasites carrying large plasmid copy numbers ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.5-0.75 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen). Lipofection was done using small volumes of Opti-MEM (200 μL ...
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bioRxiv - Microbiology 2024Quote: ... The linearized plasmid was in vitro transcribed using the mMESSAGE mMACHINE T7 Transcription Kit (ThermoFisher, Waltham, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and C952 Fab plasmids were co-transfected in Expi293F cells using the ExpiFectamine transfection kit (ThermoFisher Scientific, A14525) in a 1:1.5:1:1.5 ratio ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid was transfected into HEK293 cells at 70% confluency using Genejuice (Fisher Scientific 70-967-3) at a 1:3 ratio (DNA ...