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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... then these were each inserted into the lentiviral vector pLenti6/V5-DEST plasmid (Invitrogen, gift from Torsten Wittmann). These three truncation constructs are resistant to the NuMA sgRNA ...
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bioRxiv - Cell Biology 2019Quote: ... neurons were transfected with 2 mg indicated plasmids per well (12-well plate) using Lipofectamine 2000 reagent (Invitrogen). Neurons were fixed at DIV18 with 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using jetOPTIMUS as recommended by the manufacturer Polyplus (Illkirch, France) (Thermo Fisher Scientific, Waltham, MA). Selection of stable cell lines was carried out with geneticin ...
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bioRxiv - Developmental Biology 2021Quote: The βactinhsp70:KalT4;cmlc2:eGFP construct was generated by combining four plasmids using the Multisite Gateway system (Invitrogen): p5E-bactinhsp70 ...
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bioRxiv - Immunology 2021Quote: Ab heavy and light plasmids were co-transfected at a 1:1 ratio into HEK-293F cells (Thermofisher) using PEI Max (1 mg/mL ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were transfected with FLAG-Empty Vector (FLAG-EV) and FLAG-SP140 plasmids using Lipofectamine 2000 (Invitrogen) for 48 hours ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were transfected into Sf9 insect cells (2×106 cells/ml in 2ml) using Cellfectin II Reagent (Gibco) along with BestBac 2.0 v-cath/chiA Deleted Linearized Baculovirus DNA (Expression Systems ...
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bioRxiv - Immunology 2020Quote: ... 293T-gp96-hFc overexpressing cell line was constructed by transfected 3.1-gp96-hFc plasmid with Higene (Applygen, China) and selected with 200μg/ml hygromycin B (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... V5-APEX2 alone or IPMK-V5-APEX2 were cloned into the pcDNA™5/FRT/TO plasmid (Invitrogen). Flp-In™ T-REx™-293 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Sense RNA was synthesized from the XhoI-linearized plasmid using the Ambion mMessage mMachine T7 transcription kit (Invitrogen). 20-50 pg of capped furinb RNA was injected into the yolk of embryos from s741 heterozygotes incrosses at the 1-4 cell stage ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of early region of MCV genome-expressing plasmid (ER21) or pLenti6.3 empty control vector and pRL.TK (Renilla luciferase) using Lipofectamine 3000 Transfection reagent (# L3000015, Invitrogen) according to the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3′UTR) was amplified from the plasmid pUSER016-T7-MglE using Phusion Hot Start polymerase (ThermoFisher Scientific) and the primers linear_cassette F and linear_cassette R ...
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bioRxiv - Molecular Biology 2020Quote: ... The pp71-expressing plasmid (pCGN-pp71) was transfected in NTera2 cells using lipofectamine-3000 transfection reagent (Invitrogen™) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 1µg of each plasmid was linearized with SpeI and in vitro transcribed with T7 RNA Polymerase (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were seeded in 6-well plates and transfected with CFTR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 µg of pLVX-Nsp1/Nsp2 plasmids were transfected using 3.5 µL of Lipofectamine 3000 (GIBCO, Life Technologies) in HEK293T cell line ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 µg of pLVX-Nsp1/Nsp2 plasmids were transfected using 3.5 µL of Lipofectamine 3000 (GIBCO, Life Technologies) in HEK293T cell line ...
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bioRxiv - Cell Biology 2022Quote: ... This plasmid together with psPAX2 and pLVX-Luc2 were transfected into 293T cells using Lipofectamine 3000 Reagent (ThermoFisher) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the heavy and light chains were co-transfected into FreeStyle™ CHO-S™ cells (Invitrogen) in serum free FreeStyle™ CHO™ Expression medium ...
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bioRxiv - Cell Biology 2019Quote: The pPfBiP-Ty1overexpression plasmid was prepared first by generating cDNA using the SuperScript III reverse transcriptase kit (Invitrogen) using primer P14 ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were allowed to attach overnight prior to transfection of plasmid DNAs (0.1-0.2 µg/well) using Lipofectamine 2000 (Invitrogen) and Opti-MEM (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... wild type S2 cells were seeded at a density of 7.5×105 cells/ml and incubated at 25°C overnight before being co-transfected with 1.0 µg of each respective plasmid and 0.25 µg of pCoHygro (Invitrogen) using FuGENE HD (Promega Corporation ...
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bioRxiv - Bioengineering 2019Quote: ... was created by ligating a 1.1 kb ZeoR expression cassette PCR amplified from plasmid pSV40/Zeo2 (ThermoFisher Scientific) using 5’ phosphorylated primer pair ZeoMluIFor/ZeoMluIRev ...
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bioRxiv - Biochemistry 2019Quote: ... and plasmid pBMDEL-HTT DNA were separately diluted in serum-free OptiMEM complexation medium (Thermo Fisher, Cat. 31985062) at 10% of the total production volume in a ratio of 1 μg DNA to 1.2 μL FectoPro to 0.5 μL Booster per mL cell culture ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were allowed to attach overnight prior to transfection of plasmid DNAs (0.1-0.2 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen) within a small volume of Opti-MEM (200 μL ...
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bioRxiv - Microbiology 2019Quote: ... 150 ng of plasmids were used for transfection experiments with the aid of Lipofectamine 2000 (Invitrogen, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... and 50 ng of Acr plasmids were transiently transfected in a 24-well format using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The expression plasmids were purified and transformed into HEK cells (FreeStyle™ 293-F cell line, Life Technologies) as previously described(Moremen et al ...
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bioRxiv - Biochemistry 2020Quote: ... and transfected the cells with 2000 ng of plasmid DNA and 6 μl of Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2019Quote: ... The designed plasmids were incubated at 37°C overnight with TopOne Shot cells (Thermofisher Scientific cat no. C404010) on LB Agar Bacterial resistant plates (50 μg/ml Spectinomycin Sigma Aldrich cat no ...
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bioRxiv - Cancer Biology 2019Quote: ... StagBFPEP-miR-E shControl or StdTomatoEZ shControl together with pMD2G and psPAX2 packaging plasmids using Lipofectamine 2000 (Invitrogen). Supernatants containing lentiviral particles were used to infect MDA-LM2 cancer cells overnight in the presence of 8 μg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... virus envelope plasmid pCMV-VSV-G and appropriate transfer plasmid were mixed at a molar ratio of 1:0.54:1.26 in 3ml Opti-MEM (Gibco), using a total of 10 pmol plasmid per 150mm plate ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid was linearised using NotI (ThermoScientific) and capped mRNA was synthesised using mMessage Machine SP6 kit (Ambion). Embryos from AB zebrafish were collected at one-cell stage and injected with 10 pg optofYAP mRNA.
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bioRxiv - Immunology 2021Quote: ... VSV-g or BaEV 22 and the plasmid coding for the vector of interest using lipofectamine LTX (Invitrogen). 48hrs later the virus-containing supernatant from HEK-293T cells was concentrated 100-fold using Lenti-X concentrator (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... 3’UTRs were in vitro transcribed from 1μg of linearised plasmids using MEGAscript T7 Transcription Kit (Invitrogen, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91 (1.33μg) using 10μl of Lipofectamine 2000 (Invitrogen #11668-027) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Ce-npr-5a/pcDNA3.1 or empty pcDNA3.1 (negative control) plasmid using Lipofectamine LTX and Plus reagent (Invitrogen, UK). After transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Electroporation of plasmid DNA was conducted in resting CD4+ T cells by using Neon™ Transfection system (Invitrogen) through the suggested protocol for PBMC according to the manufacturers’ instructions ...
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bioRxiv - Bioengineering 2021Quote: ... a Thermo Fisher Scientific GeneJET Plasmid Miniprep kit was used according to the manufacturer’s recommendations (Thermo Scientific, Lithuania). Digestion with restriction enzymes was conducted according to the supplier’s instructions (Thermo Fisher Scientific Waltham ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... pEGFP-N1-M1 and pEGFP-N1-M3 plasmids were transfected into cancer cell lines using LipofectamineTM 3000 (Invitrogen). Six hours after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were generated with pCMV-VSVG and psPAX2 packaging plasmids according to the manufacturer (Thermo Fisher Scientific). Viruscontaining supernatant was collected 48 hours post transfection and used at a multiplicity of infection of 5 ...
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bioRxiv - Neuroscience 2021Quote: The HEK293T cells were divided into four groups and transfected with plasmids by Lipofectamine 2000 (11668-027, Invitrogen). The four groups (tTAN ...
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bioRxiv - Neuroscience 2020Quote: ... The in-vitro transcription reaction was performed on linearized plasmid using the mMessage mMachine SP6 Transcription Kit (ThermoFisher), and the RNA was purified using a Mini Quick Spin Column (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... synthesized ORF8 sequence was amplified by PCR and inserted into a pFastBac HT A plasmid (Thermo Fisher Scientific). Recombinant pFastBac-ORF8 plasmid was transformed into DH10Bac-competent cells to obtain Bacmid-ORF8 ...
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bioRxiv - Cell Biology 2021Quote: ... The next day the cells were transfected with a plasmid encoding ZIPT-2.3 or pcDNA-3.1(+) (a vector only control) using Lipofectamine 2000 (Invitrogen). After 48 hours ...
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ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ... The plasmids were transfected into 293FT cells (Thermo Fischer Scientific, R70007) using TurboFect™ (Thermo Fisher Scientific, R0533). Cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with plasmid DNA (2.5 μg/106 cells) or siRNA (50 nM) using Lipofectamine 2000 (Invitrogen) or Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: HEK293 or HeLa cells were co-transfected with pBABED and pX335 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...