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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and cDNA was quantified using a Qubit 1xdsDNA HS assay kit (cat#: Q33231) on a QuBit 4.0 fluorometer (Invitrogen, ThermoFisher Scientific). Libraries with unique indices were then pooled in equimolar ratios before sequencing.
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bioRxiv - Microbiology 2024Quote: ... The DNA concentration was measured using a Qubit fluorometer and a dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA), and purity was determined by measuring the A260/A280 absorbance ratio with a NanoDrop spectrophotometer (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 14.000 RPM) and protein concentration of collected supernatants measured using the Pierce BCA Protein Assay Kit (#323227, Thermo Scientific, 23227). The samples adjusted to contain equal amounts of protein (10 μg or 12 μg ...
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bioRxiv - Physiology 2024Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The DNA concentration was measured on a Qubit 3.0 fluorometer with the Qubit dsDNA BR assay kit (Thermo Fisher Scientific) and the DNA quality was measured by gel electrophoresis on an BioRad Gel Doc EZ Imager (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... carterae and HEK293T cells were firstly determined using Equalbit RNA HS Assay Kit (Vazyme Biotech, Nanjing, China) and Qbit (ThermoFisher). An aliquot of each RNA sample (∼100 ng ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of the conjugated oligonucleotide DNA and antibodies were quantified using a BCA Protein Assay Kit (Thermo Scientific 23225) and Nanodrop (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... The protein content of the samples was quantified using the Pierce™ 660 nm protein assay kit (Thermo Fisher, US) with BSA as the standard ...
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bioRxiv - Microbiology 2023Quote: ... The DNA was eluted in 60 uL 1x TE buffer and quantified using a Qubit dsDNA HS assay kit (Invitrogen). DNA libraries were prepared using a KAPA HyperPlus kit (Roche) ...
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bioRxiv - Neuroscience 2023Quote: The protein concentration of the cell lysates was determined with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were sonicated and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, cat.no. 23225) for subsequent analyses.
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bioRxiv - Biophysics 2023Quote: ... The final protein concentration was measured with the Micro BCA Protein Assay Kit (#OI191202, Thermo Fisher Scientific, Waltham, MA, USA). Protein purity was verified by SDS-PAGE and silver staining (Fig ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Quantification of cleaned libraries were measured using a Quant-iTTM PicogreenTM dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Germany). Based on the concentration ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 activity was assessed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Scientific, #C10427) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Hydrogen peroxide production in mitochondria isolated from the renal cortex was measured using a commercially available Amplex Red H2O2 assay kit (Invitrogen), as previously described (Forbes et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracted from the cells was dissolved in 0.2 M potassium hydroxide aqueous solution overnight and quantified using Pierce BCA Protein Assay Kit (PI23225, Fisher Scientific). Extracted metabolites are then resuspended in HPLC-grade water containing 5 μM 1,4-piperazinediethanesulfonic acid (PIPES ...
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bioRxiv - Cell Biology 2023Quote: ... The sample concentration was determined using the Qubit dsDNA BR Assay Kit on a Qubit 4 Fluorometer (Thermo Fisher Scientific). Purified PCR products adjusted to 25 ng/µl were sequenced using the Amplicon-EZ service at GeneWiz (Azenta Life Sciences ...
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bioRxiv - Systems Biology 2023Quote: We quantified Protein synthesis rate with the “Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit” (Thermo Fisher Scientific), with minor adaptations from the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Nascent protein synthesis was measured with the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit (cat. no. C10428, ThermoFisher). Cells were plated in 12-well plates (Cell Treat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: Mitochondrial depolarization was assessed on cells exposed to Venetoclax for 24h using the MitoProbeTM JC-1 assay kit (ThermoFisher, M34152) following manufacturer recommendations ...
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bioRxiv - Microbiology 2023Quote: ... and a Microplate Reader SH-9000Lab (Corona Electric, Hitachinaka, Ibaraki, Japan) with a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: The potential phospholipase A2 (PLA2) activity of Dr20/22 was evaluated using the EnzChek™ Phospholipase A2 Assay Kit (Invitrogen). Fresh or frozen (stored at -80 °C ...
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bioRxiv - Plant Biology 2023Quote: ... DNA quantification was carried out using a Qubit fluorometer with the dsDNA HS assay kit (Thermo Fisher Scientific, MA, USA), and concentrations were adjusted to 10 ng/μl for all samples ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA concentration of each sample was quantified using the Quant-IT Broad Range RNA Assay Kit (ThermoFisher Scientific; Q10213) and a CLARIOstar Plus plate reader (BMG LabTech) ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were then purified using 0.6x VAHTSTM DNA Clean Beads and were quantified by Qubit dsDNA HS assay kit (Invitrogen, Q32854).
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bioRxiv - Neuroscience 2023Quote: ... Following double-sided size selection by bead-purification the libraries were quantified with Qubit dsDNA HS assay kit (ThermoFisher #Q32851) and pooled for sequencing on NovaSeq6000 (SP 2 x 100bp).
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and processed for Click-iT EdU detection according to manufacturer’s protocol (Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit; Thermo Fisher). All samples were analyzed and sorted using an Aria Fusion (Becton Dickinson ...
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bioRxiv - Genomics 2024Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit according to manufacturer’s guidelines (Thermo Fisher; Cat. No. 23225). Protein samples were mixed with 4X NuPAGE™ LDS Sample Buffer (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes, Life Technologies, Rhenium, Modi’in, Israel) and quantification was performed using the Agilent DNA HS kit and Bioanalyzer 2100 ...
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bioRxiv - Bioengineering 2023Quote: ... The sample’s DNA was extracted using phenol-chloroform and kept at −20°C until applying the PicoGreen dsDNA assay kit (Invitrogen). Total sGAGs of the samples were obtained via papain (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and quality of RNA samples were evaluated using the Qubit RNA IQ Assay Kit (ThermoFisher Scientific, catalog # Q33221) and its concentration were evaluated using Qubit RNA HS Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the protein concentration in the supernatant was determined using a BCA Protein Assay Kit (Cat# 23225, Thermo Fisher Scientific). Afterward ...
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bioRxiv - Cell Biology 2024Quote: ... scATAC-seq libraries were amplified with 12 and 7 PCR cycles and quantified with the High Sensitivity dsDNA Quanti-iT Assay Kit (ThermoFisher) using a Synergy HTX microplate reader (BioTek ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer and boiled for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Quantification of the purified amplicons was performed using the Qubit dsDNA HS Assay Kit on the Qubit 3.0 Fluorometer (ThermoFisher Scientific). For the preparation of template-positive Ion PGM™ Template OT2 400 Ion Sphere™ Particles (ISPs ...
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bioRxiv - Neuroscience 2024Quote: ... and the success of the gene editing method was assessed using the T7 Endonuclease 1 (T7E1) assay kit (Invitrogen, Germany). Positive individuals from the resulting F1 generation were then crossed with wild-type fish to produce the F2 generation which were screened again with T7E1 assay ...
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bioRxiv - Microbiology 2024Quote: ... and DNA concentrations were measured using the Qubit™ dsDNA High-Sensitivity assay kit (Thermo Fisher Scientific, cat. no. Q32851).
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bioRxiv - Cancer Biology 2024Quote: ... Isolated DNA was quantified using the Invitrogen™ Qubit™ dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, cat# Q32854). Sequencing libraries were prepared from 10 ng gDNA using the Ion Torrent™ Oncomine™ Comprehensive Assay v3C (OCAv3C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA concentration was quantified using the Invitrogen™ Qubit™ RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, cat# Q32852). DNA digestion was performed using the Invitrogen™ ezDNase™ enzyme (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA concentration for immunoprecipitated and input control samples were measured with a Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher Scientific). 5 ng of DNA for each sample was then used with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cell lysis was determined in cell-free supernatants using the LDH Cytotoxicity Assay Kit (Thermo Fisher Scientific, Waltham, MA). Both DPP4 and LDH activities obtained from cell-free supernatants were normalized to the CFU counts.
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bioRxiv - Cell Biology 2024Quote: ... The 20 μg of extracted proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, WA, USA) at 500 ng/μL ...
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bioRxiv - Neuroscience 2024Quote: ... prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). 10µg of proteins were resolved using SDS-PAGE (4-12% Bis-Tris precast gels in buffer MES) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in the tissue lysates were determined by Pierce™ BCA protein assay kit (Thermo Scientific, Cat# 23227, USA). Protein lysates were suspended in sample buffer containing NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Biophysics 2024Quote: ... Protein concentrations were determined using a BCA protein assay kit (Pierce) or by absorbance at 280 nm in a Nano Drop spectrophotometer (ThermoFisher) used in cuvette mode ...