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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... donkey Anti-Rabbit (Invitrogen; A10042 or Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-GFP (Invitrogen), (1:300) ...
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bioRxiv - Biophysics 2021Quote: ... Both Fn39-10 S1417C mutant and its inactive version were biotinylated with Maleimide-PEG11-Biotin at residue 1417 (ThermoFisher Scientific) in PBS.
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bioRxiv - Molecular Biology 2021Quote: ... Biotin was attached to cross-linked RNA duplexes by incubating at 37°C for 1.5 h with 150mM Click-IT Biotin DIBO Alkyne (Life technologies) under constant agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were recovered 20 hrs biotin supplementation and were lysed in RIPA buffer with 1x Halt Protease Inhibitor (ThermoFisher) for 45 minutes at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... PltB and Neu5Gc-IgY was achieved by NHS mediated biotin tagging at 20:1 molar ratio as per manufacturer’s protocol (Thermo scientific, EZ-Link Sulfo-NHS-LC Biotin ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of DOT1B-Myc-BirA* was induced by addition of 5 ng/ml tetracycline for 24 h and cells were further incubated for 24 h with 50 µM D-biotin (Invitrogen) and 5 ng/ml tetracycline ...
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bioRxiv - Genomics 2020Quote: ... The DNA labeled with biotin was purified using 100 μL of 10 mg/mL Dynabeads My One T1 Streptavidin beads (Invitrogen). The washed beads were transferred to the sample tube ...
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The PLpro and 3CLpro proteins were biotinylated by minimal biotinylation approach with the EZ-LINK Sulfo-NHS-LC-LC-biotin reagent (ThermoFisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... cDNA was fragmented and labeled with a 3’ biotin tag before quantifying gene expression using Affymetrix custom tiling arrays (A-AFFY-116 - Affymetrix Custom Array - S ...
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bioRxiv - Bioengineering 2019Quote: ... These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific). Excessive biotin was removed by buffer-exchange into PBS ...
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bioRxiv - Cell Biology 2019Quote: SiRNA-treated and PDGF-BB-stimulated cells were washed twice with cold PBS and surface proteins were biotinylated with 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Scientific) for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo scientific) or fluorescent secondary antibody to visualize proteins ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were then reacted for 2 h at RT with the following click reagents: 50 μM biotin-alkyne (ThermoFisher Scientific), 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA ...
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bioRxiv - Biochemistry 2020Quote: ... was prepared by PCR from plasmid SB649 (20) using primers 5′-biotin-GTTGGGTAACGCCAGGG-3′ and 5′-Alexa488-GGAAACAGCTATGACATG-3′ (IDT) and Platinum Taq DNA Polymerase (Invitrogen). The PCR product was purified using DNA SizeSelector-I SPRI magnetic beads (Aline Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... S1 subunit and the S protein (InVivo Biotech) were biotinylated using 10 molar excess of NHS-LC-Biotin (Thermo Scientific). Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2 ...
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bioRxiv - Biophysics 2021Quote: ... The other end was ligated to a ∼190 bp biotin-labeled fragments created similarly by ApaI restriction of a 375 bp PCR amplicon of plasmid pBluKSP containing biotin-11-dUTP (Invitrogen, Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Cohesive DNA ends were biotinylated by the addition of 7.6 μl of the biotin fill-in mixture prepared in 1 × NEBuffer 2.1 (0.025 mM dCTP (Thermo Fisher; Cat#: R0151), 0.025 mM dGTP (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubules contained ∼10% biotin-tubulin for attachment to streptavidin-coated cover slip and ∼10% Alexa Fluor 488 (Thermo Fisher Scientific) tubulin for visualization ...
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bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and HAΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated with a 20-fold excess of 10 mM EZ-Link NHS-LC-biotin (ThermoFisher) and unbound biotin was removed using a Zeba Spin Desalting Column (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM Aminoguanidine and 2.5 mM Sodium Ascorbate the reaction allowed to proceed for 6h at room temperature before removal of the biotin excess using Zeba desalting columns (7kDa cut-off, Thermofisher). The samples were then combined altogether at a 1:1:1 ratio within one replicate ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with ice-cold PBS (pH 8.0) three times and incubated with 1 mg/ml Sulfo-NHS-LC-Biotin (ThermoFisher Scientific)/PBS for 2 min at room temperature to initiate the reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Protein pellets were resuspended by sonication in 80 μL of resuspension buffer containing 10 μM EZ-Link HPDP-Biotin (ThermoFisher), diluted with 320 μL of Triton buffer containing 10 μM HPDP-Biotin ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled LDMNCs were incubated with Biotin Binder Dynabeads at a ratio of 4 beads per input cell (Life Technologies) and the lineage-positive cells were depleted using a magnetic particle concentrator according to the manufacturer’s instructions (Dynal MPC-6 ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated in 5 ml of PBS containing 5 mg EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The labelled lysate was then either “clicked” on with TAMRA-alkyne or biotin-alkyne using copper catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry reaction protocol supplied by ThermoFisher. Biotin-labelled samples were then immunoblotted and visualized with Streptavidin-HRP antibody on an iBright™ FL-1500 imaging system (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: ... permeabilised cells were subjected to the Click-It reaction to conjugate biotin to incorporated EdU (Invitrogen, as per manufacturer’s protocol). Cells were washed 2X in PLA washing buffer and resuspended in 50 μl of each primary antibody made up in washing buffer (elongating RNA Polymerase II ...
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s protocol and excess NHS-biotin was removed with 0.5 mL Zeba Spin Desalting Columns (Thermo Fisher, 87766). Bispecific CSANs were then formed using the biotinylated αCD3 scFv monomer and the αB7-H3 scFv monomer at a 1:1 ratio ...
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bioRxiv - Biochemistry 2020Quote: ... and excess biotin reagent was removed using Zeba spin desalting column following the manufacturer’s recommendation (Thermo Fisher Scientific, Waltham, MA). The biotinylated protein samples to be used as ‘load’ in the BLI experiments were purified in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with ice-cold PBS containing 1mM CaCl2 and 0.5mM MgCl2 and incubated with 1mg/ml Sulfo-NHS-SS-biotin (Thermo Scientific) for 15 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated baits that had been either purified or dialysed against PBS to remove excess free D-biotin were immobilised in streptavidin-coated 96-well microtitre plates (NUNC). Preys were incubated for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 30 min at room temperature (RT) with 0.5 mg/ml Premium Grade EZ-link Sulfo-NHS-LC-Biotin (Thermo Scientific) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo Scientific) or fluorescent secondary antibodies with species-specific Alexa Fluor-488 or -568 (Invitrogen A-11039 or A-11011 ...
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bioRxiv - Cell Biology 2022Quote: ... then the biotin-depleted “flow-though2” was incubated with 100 μL Dynabeads™ M-280 Streptavidin (Thermo Fisher Scientific, 11205D) for 2 h at 4°C with gentle rotation ...
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bioRxiv - Genetics 2019Quote: ... The overhangs generated by the restriction enzyme were filled-in by adding a mix of 0.4 mM biotin-14-dATP (Life Technologies), 10 mM dCTP/dGTP/dTTP (0.75 µl of each dinucleotide) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... washed twice with DPBS and incubated with 0.25 mg/ml of EZ-Link Sulfo-NHS-SS-Biotin cross-linking reagent (Thermo # 21331) in DPBS supplemented with 0.1 mM oxidized glutathione (Thermo Fisher) for 30 min at room temperature under constant rocking ...
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bioRxiv - Genomics 2020Quote: ... The DNA overhangs were filled with biotinylated nucleotides by addition of 100 μl pre-mix (25 μl 0.4 mM Biotin-dATP (Invitrogen, #19524016), 25 μl 0.4 mM Biotin-dCTP (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: The p-azido phenylalanine incorporation was confirmed by biotinylation of azido group using EZ-link Phosphine-PEG3-Biotin (Thermo Fisher) (Agard et al ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2020Quote: The supernatant was aspirated and the cells were rinsed with cold PBS three times and then treated with 0.1 mg/ml EZ-linkTM Sulfo-NHS-LC-Biotin (Thermo Scientific) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear RNA was fragmented by base hydrolysis and enriched for biotin-labelled RNA with Streptavidin beads (Dynabeads M280 Streptavidin, InVitrogen). Nascent biotinylated RNA was ligated with 3’-VRA RNA adaptor (5’Phos-GAUCGUCGGACUGUAGAACUCUGAAC-3’invdT) ...
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bioRxiv - Biophysics 2022Quote: ... The biotin-PEG layer was functionalized by incubating for 15 min with a 0.2 mg/mL solution of neutravidin (Thermo Scientific). Channels were washed with 1 mL of HB to remove excess neutravidin ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... at 37 °C and biotin enrichment was done using 20 ul Dynabeads™ MyOne™ Streptavidin C1 beads (Invitrogen #65001). Libraries were prepared with the NEBNext Ultra II Library Preparation Kit (NEB #E7103) ...
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bioRxiv - Microbiology 2022Quote: ... The membrane containing the biotin alkyne labeled proteins was incubated in a solution containing 1% BSA with streptavadin-HRP (Invitrogen) for subsequent visualization.
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bioRxiv - Immunology 2022Quote: ... The excess biotin of biotinylated mAbs was removed with 7k molecular weight-cutoff (MWCO) Zeba spin desalting columns (Thermo Scientific). Plates were coated with 2 μg/ml RBD antigen overnight at 4°C ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... Cells were washed twice in ice-cold PBS prior to immersion in ice-cold PBS (pH7.7) containing 200µg/ml biotin (Thermo Scientific #A39258) for 30 min with gentle agitation at 4 OC ...