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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (ThermoFisher Scientific; #31430 or #32460) (1:5000 - 10,000).
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Cancer Biology 2023Quote: ... the sample was washed 3 x 5 min with PBS and stained with secondary antibodies Anti-Rabbit-568 (1:500, ThermoFisher A-21069), Anti-Mouse-488 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... 1:100 in AK Diluent) and secondary antibodies (anti-goat 546, Thermo Fisher, #A-11058, 1:400; Alexa Fluor 488 anti-rabbit, Thermo Fisher, #A-21206 ...
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bioRxiv - Cancer Biology 2023Quote: ... then the cells were washed with PBS and incubated in secondary antibody diluted in PBSAT (Alexa Fluor donkey anti–rabbit 488, 1:1000, Life Technologies; A21206) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then washed in PBS before being incubated in Alexa fluorophore secondary antibodies (donkey anti-rabbit, Thermo Fisher Scientific #A-21206, 1:1000) for 1 h at room temperature ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... for two hours at room temperature Sections were incubated in the following primary antibodies overnight at 4 °C: ERβ (rabbit, 1:200, Invitrogen PA1-311) and NeuN (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then immersed in diluted blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse IgG1, Invitrogen / Thermo-Fisher), each at 1:800 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed and incubated in the secondary antibody cocktail (1:200, anti-mouse; Alexa-594, Cat. # A11005 and 1:200, Alexa-488 anti-rabbit, Cat. # A11008; ThermoFisher Scientific, USA) for 4- 5hrs at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS and incubated in the secondary antibody solution (1:200, Alexa-488 anti-rabbit; A11008, ThermoFisher Scientific, USA) for 4-5hrs followed by three washes in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were washed with DPBS for 5 min three times and then incubated with DyLight 800-labeled donkey anti-rabbit secondary antibody (Invitrogen, SA5-10044) at 1:1000 dilution and Alexa Fluor 647-labeled goat anti-rat secondary antibody (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... and LIMYB-32-L4-isolated nuclei were immunoprecipitated using rabbit polyclonal anti-GFP - IgG antibody (Thermo Fisher Scientific, MA, USA, cat. # A11122) and captured with protein-A agarose beads (InvitrogenTM ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated with a 1:500 dilution of goat anti-rabbit IgG DyLight 488 conjugated secondary antibody (Thermo Fisher Scientific #35552) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody goat anti-Rabbit DyLight 680 (ThermoFisher, cat. no. 35568) or/and donkey anti-Goat DyLight 680 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with the appropriate secondary antibodies (anti-rabbit-A488 Life Technologies, 1:1000; anti-mouse-A568 Life Technologies, 1:1000) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (three times in 0.3% Triton X-100 in 1x PBS for 15 min) and incubated with Alexa Fluor 488 goat anti-rabbit secondary antibody (1:1000, Thermo Fisher Scientific) and Alexa Fluor 546 goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... overnight at 4°C and thenincubated for 45 minutes at room temperature with the secondary antibody goat anti-rabbit IgG Alexa Fluor488 (1:300, #A11008, Thermo Fisher Scientific) and phallodin (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were Alexa Fluor 488 and Alexa Fluor 594 donkey anti-rabbit and donkey anti-goat (all Life Technologies; 1:300), Alexa Fluor 594-conjugated streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in 10 ml Blotto + goat α-rabbit poly-horse-radish peroxidase secondary antibody (Invitrogen; 1:10,000 dilution) for 1-2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Table S4) using the HCR v.3.0 protocol (Molecular Instruments) described above and a rabbit anti-GFP antibody (Invitrogen, A11122, Table S4) with subsequent secondary goat anti-rabbit conjugated with AF488 (ThermoFIsher ...
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bioRxiv - Microbiology 2023Quote: ... the samples treated with primary antibody and washed in PBS-Tw were incubated with goat anti-rabbit IgG conjugated Alexa Fluor 488 (Life Technologies, USA) diluted 1:500 in the blocking solution for 90 minutes in RT followed by rinsing with PBS-Tw (three times for 10 min at RT in the dark) ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with 1:500 goat anti-rabbit IgG (H+L) secondary antibody tagged with Alexa Fluor 647 (Invitrogen, A-21244) + 1:50 SiglecF-Alexa Fluor 488 (BD ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used for hiPSC-derived neurons and microglia were as follows: anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 488 (Invitrogen, 1:1000), anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: Three vibratome slices from three goat eyes were incubated for 4 hours in 5% BSA blocking solution without permeabilization agent, overnight in GFAP primary antibody (rabbit origin, (Invitrogen, PA5-16291) at 1:200 in blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... retinas were incubated for 2 hours at RT with 546-conjugated anti-rabbit IgG antibody (1/300; Life Technologies, Saint Aubin, France). Cell nuclei were labelled with Draq5 (1/500 ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Cell Biology 2022Quote: ... followed and the slides were incubated with secondary antibody (1:400 in blocking buffer, donkey a-rabbit Alexa 488 antibody, Invitrogen A-21206; or goat a-mouse Alexa488, Invitrogen A-11001) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed with TBST and incubated with secondary antibodies in blocking buffer [goat anti-rabbit (cat# A32731; Invitrogen, Waltham, MA, USA) or goat anti-mouse (cat# 8890 ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used were as follows: Alexa Fluor 488 either anti-mouse or anti-rabbit IgG (A11001, A21206, Thermo Fisher Scientific), Alexa Fluor 647 either anti-mouse or anti-rabbit IgG (A21236 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed three times with TBS-0.1% Tween 20 and incubated for 1 hour with anti-mouse/rabbit secondary antibodies (ThermoFisher Scientific, Waltham, MA). Subsequently ...
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bioRxiv - Developmental Biology 2023Quote: ... the signals were detected using a donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 568 dye (Invitrogen, A10042; 1:1000 dilution) as described previously [40].
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and incubated for 45 min with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Donkey anti-Rabbit IgG, (#A10042, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times with PBS and labeled with secondary antibodies (goat anti-rabbit/human conjugated to Alexa Fluor 647; 1:1000; Invitrogen, #A21246/A21445) and FluoTag-X4 anti-GFP conjugated to Atto488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 546 goat anti-rabbit IgG and Alexa Fluor 546 goat anti-mouse IgG secondary antibodies were purchased from Invitrogen (Carlsbad, CA).
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bioRxiv - Developmental Biology 2023Quote: ... Cells were centrifuged at 1400 rpm for 4 minutes and incubated with Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (ThermoFisher, MA5-14520) at room temperature for 30 minutes ...
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bioRxiv - Genetics 2023Quote: ... the slides were incubated at 37 °C for 30 min with a 1:1000 dilution of secondary antibody goat anti-rabbit IgG 594 (Molecular Probes, A11012) and goat anti-mouse IgG 488 (Molecular Probes ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 1 h with an Alexa594-conjugated goat antibody to rabbit IgG (5 μg/ml; A11012, Thermo Fisher Scientific) and a biotinylated donkey antibody to mouse IgG (10 μg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 1 h with an Alexa488-conjugated goat antibody to rabbit IgG (5 μg/ml; A11034, Thermo Fisher Scientific) and a biotinylated donkey antibody to mouse IgG (10 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and for 30 min with secondary Alexa488/647-conjugated antibodies (anti-mouse-/anti-rabbit-IgGs: Thermo Fisher Scientific, A-21202/A-31573). Sections were imaged using an Evos FL microscope and marker expression was quantified using CellProfiler 63.
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... the cells were washed 3 times with DPBS and incubated with secondary goat anti-rabbit antibody (Alexa Fluor 488; Thermo Fisher Scientific) at a 1:500 dilution in 1X PBS containing 1% bovine serum albumin and 0.3% Triton X-100 for 1-1.5 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were subsequently washed with PBS and incubated with fluorophore-conjugated secondary antibodies in blocking solution [anti-rabbit IgG Alexa Fluor™ 594 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig, and goat anti-rabbit were from Invitrogen, MA, USA) were used at 1:500.
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bioRxiv - Immunology 2023Quote: ... The resulting cells were then stained by a FITC-conjugated anti-rabbit IgG secondary antibody (4300581, eBioscience) and analyzed on an Attune NxT Flow Cytometer (Thermo Fisher Scientific). The data were processed by Flojo software (FlowJo ...
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bioRxiv - Pathology 2023Quote: ... appropriate secondary antibodies were treated for 1-2 hours in the dark (AF555 anti-Rabbit, AF555 anti-Rat, AF647 anti-Rat (Invitrogen, Waltham, MA)) and washed in PBS containing 0.01% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for another 24 hours in the solution containing secondary antibodies (1:1000, goat anti-rabbit, Thermo Fisher #A32731). The samples were then washed in PBS-T and mounted in Tris-HCL (pH 8.0)-buffered 80% glycerol + 0.5% DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2 hours with a secondary goat anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (Invitrogen, A-21244). Nuclei were labeled by 4-,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with 0.1% Tween in PBS and incubated with donkey anti-rabbit IgG H&L Alexa Fluor™ 568 secondary antibody (1:1000, Invitrogen, A10042) and 1 μM Hoechst prior to imaging ...