Labshake search
Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... and then emerald GFP-miR cassette was PCR amplified and subcloned into the viral expression vector pRRLsinPPT (Invitrogen). Viral particles were produced in HEK-293T cells as previously described (Thomas et al. ...
-
bioRxiv - Cancer Biology 2019Quote: Four miRs designed for targeting TLX and a negative control miR (BLOCK-iT™ RNAi Designer, ThermoFisher) were cloned into pcDNA3.1 and cotransfected with a vector expressing mouse TLX into HEK293T cells ...
-
bioRxiv - Cell Biology 2022Quote: ... senescence green detection kit (ThermoFisher, C10850) was used to label senescent cells according to the manufacturer’s specifications ...
-
bioRxiv - Cancer Biology 2023Quote: ... The ViewRNA eZL Detection Kit (Affymetrix) was used on the Bond RX immunohistochemistry and ISH Staining System with BDZ 6.0 software (Leica Biosystems) ...
-
bioRxiv - Plant Biology 2024Quote: ... and Supersignal detection kit (Thermofisher Scientific). Blot images were acquired with a UVP ChemStudio Plus gel imaging system using Vision Works software.
-
bioRxiv - Plant Biology 2021Quote: ... Detection was performed using the SuperSignal West Dura Detection Kit (Thermo Fisher) and Syngene PXi 4 imager with GeneSys software version 1.4.1.0 (Syngene).
-
bioRxiv - Immunology 2021Quote: ... The PrimeScript RT reagent kit (Invitrogen) was employed to synthesize cDNA ...
-
bioRxiv - Systems Biology 2024Quote: ... Cells were all confirmed to be mycoplasma negative using a PCR mycoplasma detection kit (Thermo Fisher Chemicals, J66117) following manufacturers instructions.
-
bioRxiv - Microbiology 2021Quote: ... Multi-segment polymerase chain reaction (MS RT-PCR) was performed using SuperScript IV One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerases (ThermoFisher, cat#12574-035) and influenza-specific universal set of primers(44 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The expression of mRNAs were quantified by RT-qPCR analysis with SYBR Green PCR Kit(Invitrogen, California, USA). β-actin was used for the normalization control ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using Thermo Script RT-PCR kit according to the instructions provided by the manufacturer (Invitrogen). Real-time PCR reactions were set up with a SYBR green power up kit (Applied Biosystems ...
-
bioRxiv - Microbiology 2021Quote: ... The RNAs were amplified using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... 10 μl of TaqMan® RT-PCR mix (TaqMan® RNA-to-CtTM 1-Step Kit, Applied Biosystems), 0.5 μl of TaqMan® RT-Enzyme Mix ...
-
bioRxiv - Microbiology 2022Quote: ... RT-PCR was done using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, ThermoFisher Scientific, Brussels, Belgium).
-
bioRxiv - Microbiology 2020Quote: ... The spike protein gene cDNA was amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... cDNA synthesis was performed using the Maxima First Strand cDNA Synthesis Kit for RT-quantitative PCR (Thermo Scientific) or NZY First-Strand cDNA Synthesis Kit ...
-
bioRxiv - Microbiology 2020Quote: ... PCR master mix containing Invitrogen SuperScript™ III Platinum™ One-Step RT-qPCR Kit (Life Technologies, USA) mastermix ...
-
bioRxiv - Microbiology 2021Quote: ... Sequencing reactions using purified RT-PCR products were performed with BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems) using standard cycling parameters ...
-
bioRxiv - Neuroscience 2022Quote: ... qPCR was performed on an Applied Biosystems 7500 RT–PCR system using the Power SYBR kit (Applied Biosystems). Cycle threshold (Ct ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was generated using the Superscript III First-Strand Synthesis System for RT-PCR Kit (Invitrogen, Waltham, MA) per manufacturer’s instructions and all qRT-PCR was performed on a CFX Connect Real-Time System (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... cDNA was generated using the Superscript First Strand Synthesis kit for RT-PCR and random hexamer primers (Invitrogen). Q-PCR was performed with SYBR Green using StepOnePlus PCR system (Applied Biosystem) ...
-
bioRxiv - Genomics 2023Quote: ... Quantitative RT-PCR reactions were assembled using the Power SYBR Green RNA-to-CT 1-Step Kit (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... cDNAs were generated from dsRNAs using a Superscript III One-Step RT-PCR Platinum Taq kit (Thermo Fisher) and appropriate segment 7 (NSP3 ...
-
bioRxiv - Microbiology 2020Quote: PCR was performed on RT+ and RT-cDNA reactions using Amplitaq Gold DNA Polymerase (Thermo Fisher Scientific) and primer pairs corresponding to each newly identified VZV transcript (Table S5) ...
-
bioRxiv - Bioengineering 2020Quote: ... plasmids bearing wild type HSA and the mutants were transfected to HeLa cells using Lipofectamine 3000 transfection kit (Invitrogen) and Opti-MEM (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... from each sample was reverse-transcribed using the qScript™ One-Step SYBR® Green qRT-PCR kit in a ViiA-7 RT-PCR system (Thermo Fisher Scientific). The GAPDH gene was used as the endogenous control ...
-
bioRxiv - Plant Biology 2020Quote: Quantitative PCR (ABI Prism 7300 Sequence Detection System, Applied Biosystems) was performed using 30 ng cDNA and the iQ SYBR Green Supermix (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2024Quote: ... the viral RNA was considered for first-strand cDNA synthesis following the protocol mentioned in the one-step RT PCR kit (SuperScript™ III kit, Invitrogen, USA) consisting of various reagents from the kit inclusive of 10 μmol of each primer ...
-
bioRxiv - Microbiology 2020Quote: ... Real-time qPCR was performed with PowerUp SYBR Green Master Mix (ThermoFisher) and a QuantStudio 5 (ThermoFisher) ...
-
bioRxiv - Cell Biology 2019Quote: ... which was ran on a StepOnePlus Real-Time platform (Thermo Fisher Scientific). To quantify gDNA the geometric mean of two primer pairs amplifying different genomic loci was used ...
-
bioRxiv - Developmental Biology 2021Quote: ... Reactions were assayed using a QuantStudio6 Real-time system (Thermo Fisher Scientific). Zebrafish specific Taqman assays (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... The real-time qPCR reactions were performed on StepOne system (Applied Biosystems). The reactions were performed in duplicates and the results were standardized with the endogenous control of TUB1 (α-Tubulin gene) ...
-
bioRxiv - Immunology 2021Quote: ... Quantitative real-time qPCR was performed using specific Taqman probes (Thermo Fisher) for TGFβ1 (Tgfb1) ...
-
bioRxiv - Neuroscience 2021Quote: ... For 96-well microtiter plates StepOne Plus real time cycler (Applied Biosystems) and for 384-well microtiter plates a 7900 HT real-time cycler (Abi ...
-
bioRxiv - Cell Biology 2022Quote: ... Real-time QPCR was carried out using SYBR Green Mastermix (Applied Biosystems) with specific primers whose efficiency had been previously checked ...
-
bioRxiv - Biochemistry 2021Quote: ... Real-time qPCR was performed on a QuantStudio 6 Flex (Applied Biosystems), using the following Taqman primers and probes ...
-
bioRxiv - Cell Biology 2022Quote: ... and assays (Table S3) were used for real-time qPCR (Thermo Fisher, Applied Biosystems QuantStudio 3 and BioRad CFX 384 ...
-
bioRxiv - Genomics 2022Quote: ... on a QuantStudio™ 5 Real-Time platform (Applied Biosystems cat.no. A34322). Relative mean expression was normalized to the expression levels of GAPDH or β-Actin.
-
bioRxiv - Neuroscience 2022Quote: ... then visualised using an E-Gel Safe Imager Real Time Transilluminator (Invitrogen) to ensure specific amplification ...
-
bioRxiv - Neuroscience 2022Quote: ... using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems). Thresholds cycles (Cts ...
-
bioRxiv - Molecular Biology 2024Quote: ... Melt curve was performed with StepOne Plus Real-Time machine (Applied Biosystems). Results were exported to csv files using the Applied Biosystems Analysis Software.
-
bioRxiv - Neuroscience 2024Quote: ... Reactions were performed in a Real-Time 7900 Fast apparatus (Applied Biosystems). Relative quantification was carried out using the 2ΔΔCT method ...
-
bioRxiv - Microbiology 2023Quote: ... determined by the ABI 7500 Real-Time software (version 1.4, Applied Biosystems), was ≤ 36 ...
-
bioRxiv - Cell Biology 2023Quote: Real-time qPCR was performed on ViiA™ 7 system (Applied Biosystems). Total volume of qPCR reaction was 20µl and it contained 10µl of 2x qPCR Master Mix Low ROX (Eurogentec) ...
-
bioRxiv - Neuroscience 2024Quote: ... using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems). Threshold cycles (Cts ...
-
bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was performed using the TaqMan system (Applied Biosystems) in a 384-well format ...
-
bioRxiv - Immunology 2020Quote: ... in a ViiATM-7 RT-PCR system (Thermo Fisher Scientific). Primer sequences are available upon request ...
-
bioRxiv - Plant Biology 2019Quote: ... RT-PCR was performed using M-MLV reverse transcriptase (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... in a Viia 7 RT-PCR machine from Applied Biosystems. All experiments were performed with least three biological replicates collected from different days ...
-
bioRxiv - Microbiology 2020Quote: ... One-step RT-PCR was performed using SuperScript III (Invitrogen), Platinum Taq HiFi polymerase (Thermo Fisher) ...