Labshake search
Citations for Thermo Fisher :
501 - 550 of 8899 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... Flag peptide and small size of non-specific proteins were removed by 20K Slide-A-Lyzer dialysis cassette (ThermoFisher Scientific, 66003) with 1L binding buffer (PBS ...
-
bioRxiv - Systems Biology 2019Quote: ... The Gateway cloning cassette was introduced immediately after the SNAP tag sequence using the Gateway vector conversion system (Invitrogen, # 11828-029). All subsequent cloning steps were performed using Gateway BP and LR reactions according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: ... between 0.5-2 mg of individual histones were combined and dialyzed into 2 M NaCl (10 K MWCO, 3-12 mL Slide-A-Lyzer Dialysis Cassettes, ThermoFisher Scientific). After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare ...
-
bioRxiv - Bioengineering 2020Quote: ... The product was then dialyzed against water using a Slide-A-Lyzer G2 10 kDa molecular cutoff dialysis cassette (Thermo Scientific) with daily water changes for 1 week ...
-
bioRxiv - Biochemistry 2020Quote: ... These fractions were pooled and dialyzed overnight into the same buffer without imidazole using a Slide-A-Lyzer Dialysis Cassette (20k MWCO, Thermo Scientific). During dialysis ...
-
bioRxiv - Bioengineering 2021Quote: ... they were dialyzed overnight using Slide-A-Lyzer G2 dialysis cassettes with a molecular weight cutoff of 10 K (Thermo Scientific). The size was determined by DLS measurements using a Zetasizer Nano ZS (Malvern Instruments Ltd. ...
-
bioRxiv - Bioengineering 2020Quote: ... followed by overnight dialysis against sterile PBS using a Slide-A-Lyzer G2 cassette with 10,000 Da molecular-weight-cut-off (Thermo Fisher Scientific). Dialyzed LNP solutions were concentrated using Amicon® Ultra centrifugal filter units with 10,000 Da molecular-weight-cut-off (Millipore) ...
-
bioRxiv - Cancer Biology 2020Quote: ... sgRNA cassettes were PCR amplified from the genomic DNA using Phusion Flash High Fidelity PCR Master Mix (Thermo Scientific, catalog#F548L). The PCR product was then end-repaired to produce blunt ends using T4 DNA polymerase (NEB M0203L) ...
-
bioRxiv - Plant Biology 2021Quote: ... Expression vectors containing the promoter-gene-fluorescent tag cassette or 3’UTR were obtained by using LR clonase-based three-fragment recombination system (Invitrogen®), the pB7m34GW/pH7m34GW/pK7m34GW/pS7m43GW/ pLOK180_pR7m34g (gift from L ...
-
bioRxiv - Immunology 2020Quote: ... Purified proteins were dialyzed overnight against PBS using Slide-A-Lyzer® dialysis cassettes (10 kDa MW cut-off, Thermo Fisher). Protein concentration was determined using the NanoDrop™ One instrument (Thermo Fisher).
-
bioRxiv - Immunology 2020Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S-2P IMAC elution fractions were concentrated to ~1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... Histology cassettes of fixed tissues were then fixed using the routine overnight non-fat cycle on the Excelsior AS (Thermo Scientific) and embedded in paraffin wax (Histoplast LP ...
-
bioRxiv - Cell Biology 2022Quote: ... The PCR template cassette together with the enAsCas12a expression plasmid were electroporated into hTERT-RPE1 cells using the Neon Transfection System (Thermo Fisher), and cells were selected with zeocin or puromycin for 2 weeks ...
-
bioRxiv - Molecular Biology 2022Quote: ... and dialyzed at 4°C overnight with dialysis buffer (20mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol, 2mM MgCl2) using Slide-A-Lyzer Dialysis cassette (Thermo Scientific 66383). 0.05% was included in all buffers when purifying Dll ...
-
bioRxiv - Plant Biology 2021Quote: ... The gene expression cassette in pJLSmart was then cloned into the destiny vector pGWB414 via recombination reactions using LR Clonase II (ThermoFisher Scientific). Vectors were confirmed by Sanger sequencing and then transformed into Agrobacterium strain 1D1249 for transient expression and agromonas assay in N ...
-
bioRxiv - Neuroscience 2021Quote: ... A Gateway cassette was added within the MCS to allow insertions of some coding sequences with Gateway Cloning System (Thermo Fisher), while others were inserted directly in the MCS by restriction digestions or with In-Fusion cloning (Clontech) ...
-
bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and dialyzed with PBS buffer pH 7.4 using Slide-A-Lyzer™ Dialysis Cassette 10K MWCO (Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Fractions containing protein as determined by the A280 absorbance were combined and dialyzed into PBS with 10k MWCO Slide-A-Lyzer dialysis cassettes (Fisher Scientific). The dialysed antibody was concentrated in a 50 MWCO Amicon Ultra Spin column (Fisher Scientific) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Dialysis was performed using Slide-A-Lyzer™ Dialysis Cassettes with a 3.5 kDa cut-off (Thermo Scientific, Rockford, IL, USA). Extracts were dialyzed three times with 150 mL S30 buffer per mL extract for 45 min at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... gra29 and sfp1 were disrupted in RHΔku80 and PruΔku80 backgrounds by co-transfecting a double gRNA pCAS9 vector targeting the 5’ and 3’ of the gene and a synthetic repair construct with 500 nt homology arms and HXGPRT-T2A-mCherry cassette (GeneArt, Life Technologies). Transfectants were selected by the addition of M/X ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
-
bioRxiv - Biochemistry 2021Quote: ... Imidazole in the eluted samples was removed through dialysis using Slide-A-Lyzer Dialysis Cassette MWCO 10K (66380, Thermo Fisher Scientific) in 3L of dialysis buffer (10mM HEPES ...
-
bioRxiv - Cell Biology 2020Quote: ... DFBS was made by dialyzing against 100 volumes of PBS (five changes in three days) using Slide-ALyzerTM G2 dialysis cassettes with cut-through MW size 2K (ThermoFisher Scientific) at 4 ⁰C to remove any potential polyamines in the FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... For designing the RNAi cassette of (firefly) luciferase (P. pyralis, NCBI accession number M15077.1) LOCK-iT™ RNAi Designer from Thermo Fisher Scientific (https://bit.ly/3qcESry ...
-
bioRxiv - Cancer Biology 2022Quote: ... It was then centrifuged at 13,300 rpm and 4 °C for 15 min and the supernatant was transferred to a “Slide-A-Lyzer™ G2 Dialysis Cassettes” (3.5K) (Thermo Scientific) and dialyzed against 3 L of dialysis buffer (20 mM Tris-Cl pH 7.3 ...
-
bioRxiv - Biochemistry 2022Quote: ... proteins stored in Tris buffer were dialyzed four times against methylation buffer (25 mM HEPES [pH 7.5], 200 mM NaCl, and 10% glycerol) using Slide-ALyzer™ Dialysis Cassettes (Thermo Scientific) with an appropriate cut-off ...
-
bioRxiv - Plant Biology 2022Quote: ... 2006) and the cassettes cloned into pENTR/D-TOPO using a pENTR™/D-TOPO™ Cloning Kit (Thermo Fisher Scientific) and subsequently pUB-DEST (Grefen et al. ...
-
bioRxiv - Synthetic Biology 2022Quote: ... successful removal of the CapR cassette was confirmed by PCR using ‘KO-Ver’ primers and DreamTaq polymerase (Thermo Scientific, Dreieich, Germany).
-
bioRxiv - Neuroscience 2022Quote: ... The solution was applied to three rounds of dialysis against 1 liter of PBS by using a Slide-A-Lyzer dialysis cassette (MWCO10,000; Thermo Fisher Scientific). The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo filter (Sartorius ...
-
bioRxiv - Plant Biology 2022Quote: ... The resulting cassette expressing the sgRNA and Cas9 was transferred into the pMpGE010 vector using LR Clonase II Enzyme Mix (Thermo Fisher) and then transformed into Tak-1 thalli ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 % penicillin/streptomycin, R16 medium and dialysis cassettes (Slide-A-Lyzer, cellulose, 100K molecular weight cut-off) were obtained from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Immunology 2023Quote: ... Each antibody with interpretable sequencing was expressed via a linear DNA cassette in Expi293F cells (Thermo Fisher Scientific, Cat No. A14527). Cell culture media was tested for binding to HIV-1 envelope ...
-
bioRxiv - Genomics 2023Quote: ... and 10% glycerol by dialysis using Slide-A-Lyzer G2 Dialysis Cassettes with 20-kDa cutoff (Thermo Fisher Scientific, no. 87737) at 4 °C overnight ...
-
bioRxiv - Plant Biology 2023Quote: ... The gene expression cassette in pJLSmart was then cloned into the destiny vector pGWB414 via recombination reactions using LR Clonase II (ThermoFisher Scientific). Constructs were confirmed by Sanger sequencing and then transformed into Agrobacterium strain GV2260 for transient expression in N ...
-
bioRxiv - Neuroscience 2023Quote: ... Purified vector fractions were dialysed against phosphate-buffered saline (PBS) overnight (Slide-A-lyzer dialysis cassette 10,000 MWCO, Thermo Fisher Scientific). Viral genome titres were quantified by quantitative RT-PCR (qRT-PCR).
-
bioRxiv - Microbiology 2023Quote: ... The EPS was filtered through a 0.22 µm filter and then was transferred to Slide-A-Lyzer dialysis cassette (Thermofisher, Cat# 66380) and placed in beaker containing Milli-Q water ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were transfected with a KLF2 expression plasmid containing a puromycin resistance marker and a DsRed expression cassette (pBRPyCAG-hKlf2-DsRed-Ip; addgene, Cat # 26277) using Lipofectamine 3000 (Thermo Scientific). Clones were selected by treatment with 2 μg/mL puromycin in normal medium that was replaced every few days ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... which contains a p35S driven β-estradiol inducible XVE cassette (Floriach-Clark et al., 2021; Kubo et al., 2013; Nakaoka et al., 2012) via a Gateway® LR reaction (Invitrogen) as the manufacture’s recommendation.
-
bioRxiv - Neuroscience 2023Quote: ... Labeled protein was filter-dialyzed against 10 L of PBS using a 3K-CutOff Slide-A-Lyzer™ Dialysis Cassette (ThermoFisher). Labeling quality was assessed by Mass Spectrometry as previously described [46].
-
bioRxiv - Synthetic Biology 2023Quote: ... proteins were dialyzed overnight using a 7 or 10 kDa molecular weight cut-off Slide-A-Lyzer dialysis cassette (Catalog No. 66380, Thermo Scientific) in high salt buffer (500 mM NaCl and 20 mM Tris ...
-
bioRxiv - Bioengineering 2023Quote: DNA fragments for construction of plasmids and expression cassettes were PCR amplified with Phusion High Fidelity DNA Polymerase (Thermo Fisher Scientific) according to the manufacturer’s manual ...
-
bioRxiv - Microbiology 2023Quote: ... the pH of the crude extract was adjusted to 5 with NaOH and dialyzed against water with a Slide-A-Lyzer Dialysis Cassette (MWCO of 3.5 kDa; Thermo Fisher Scientific). For HPLC-MS analysis ...
-
bioRxiv - Neuroscience 2023Quote: ... The fractions containing vector particles were collected and applied to three rounds of dialysis against PBS using a Slide-A-Lyzer G2 dialysis cassette (MWCO 10,000; Thermo Fisher Scientific). The dialyzed solution was finally concentrated by centrifugation through a Vivaspin Turbo 4 membrane filter (MWCO 10,000 ...
-
bioRxiv - Neuroscience 2023Quote: ... with a linear pH gradient 3-10 were rehydrated in 155 µL of the resulting mixture overnight at room temperature inside IPG Runner cassettes (cat. # ZM0008, Life Technologies). Isoelectrofocusing (first dimension separation ...
-
bioRxiv - Molecular Biology 2023Quote: ... and after 72 hours the viral particles were collected by iodixanol gradient and purified using 10K MWCO Slide-A-Lyzer G2 Dialysis Cassette (ThermoFisher Scientific). AAV6 titre was determined by Quick Titre AAV quantification kit (Cell BioLabs ...
-
bioRxiv - Biophysics 2023Quote: ... The mixture of cleavage products was dialyzed overnight at 4°C using a Slide-A-Lyzer cassette (10 kDa cutoff, Thermo Scientific) in 4 l dialysis buffer (20 mM Tris-HCl ...
-
bioRxiv - Immunology 2024Quote: ... Fractions containing the S protein were pooled and dialyzed against PBS buffer pH 7.4 using Slide-A-Lyzer™ Dialysis Cassette 10K MWCO (Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The CFTR expression plasmids were co-transfected along with a plasmid expressing 4 copies of the original or optimized (Opt) ACE-tRNA expression cassettes into HEK293T cells using Lipofectamine LTX (Thermo Fisher) as outlined above ...
-
bioRxiv - Molecular Biology 2024Quote: ... The mixture of SUMO protease cleavage products was dialyzed overnight (4 ºC, 4 L, 20 mM Tris-HCl, 150 mM NaCl, Slide-A-Lyzer→ cassette, 10 kDa, Thermo Scientific). The hexa-histidine tagged SUMO protein was separated from non-tagged authentic Mpro present in the dialysate by IMAC and collecting Mpro in the flow through ...
-
bioRxiv - Cell Biology 2024Quote: ... human BAK was cloned into a version of the retroviral Peak12-MMP vector containing an IRES-hygromycin cassette for selection in 200 μg/ml hygromycin B (Invitrogen, 10687010). The cDNAs for all BH3-only members ...