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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...
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bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
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bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibody incubations were for 1 hour at RT using fluorescently tagged antibodies from Invitrogen. All antibody dilutions were made in a solution of 1 mg/ml BSA in 0.25% Tween 20 in PBS ...
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bioRxiv - Microbiology 2024Quote: ... Vectors of sulfatase 1 and 2 tagged with myc were transfected using Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown on TSA plates at 37°C in a sealed box with Anaerogen sachets (Oxoid ThermoFisher) for 24h ...
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bioRxiv - Cell Biology 2019Quote: ... 0.3μg of GFP-tagged construct DNA were mixed with 0.7μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1μg of GFP-tagged construct DNA were mixed with 0.2μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of 6xHis-tagged proteins and Strep-tagged proteins was tested with 6xHIS TagAntibody HIS.H8 (Invitrogen Co.) and StrepMAB-Classic ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then incubated with fluorescently tagged secondary antibodies and/or fluorescently tagged Phalloidin (ThermoFisher Scientific, Waltham, MA) diluted in the blocking buffer at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg of GFP-tagged construct DNA were mixed with 0.5µg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cancer Biology 2022Quote: ... groups followed by affinity NeutrAvidin (Thermo Fisher Scientific) pulldown ...
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bioRxiv - Biochemistry 2022Quote: ... and HCA groups using Trizol RNA reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Human A549 and mouse LUAD cells were cultured in high-glucose DMEM (Life Technologies #11965-092) supplemented with 10% FBS and 1% penicillin/streptomycin (“full DMEM” ...
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bioRxiv - Biochemistry 2020Quote: Human primary dermal fibroblasts and HeLa cells were cultured in high-glucose DMEM (Thermo Fisher, #31966047) and 10% FBS (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescently tagged secondary antibodies were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Alexa 488-tagged phalloidin (ThermoFisher, 400x stock) was added at 4x working concentration and incubated for 2 days ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged Fzd4 in pcDNA3.1 (Thermo Fisher), or both Tspan12 and Fzd4 (300 ng each receptor per mL of cells) ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor-tagged secondary antibodies (Invitrogen) were matched to the primaries and incubated for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2) 1 U/μL RNaseOUT™ Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific). Hydra polyps in MCB buffer were homogenized on ice using a Dounce homogenizer ...
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bioRxiv - Immunology 2020Quote: Recombinant HIV-1 Env gp120 was expressed in Freestyle 293 cells (ThermoFisher) by transient transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... along with 1 unit of RNaseOUT Recombinant RNase Inhibitor (Invitrogen Cat. 10777019) and 1 mM MgCl2 (Thermo Fisher Scientific Cat ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
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bioRxiv - Immunology 2022Quote: ... incubated with DPBS containing 10% HI FBS and 1:1000 Hoechst 33342 (Invitrogen, #H3570) for nuclear staining and mounted onto microscope cover slips with Fluoromount G (Southern Biotech ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: Each fraction was analysed on an Orbitrap Lumos Tribrid mass spectrometer equipped with the FAIMS Pro ion mobility system interfaced with an nLC 1200 liquid chromatography system (all from Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm × 2 cm ...
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bioRxiv - Evolutionary Biology 2023Quote: The fractions were analyzed on an Orbitrap Eclipse Tribrid mass spectrometer equipped with the FAIMS Pro ion mobility system and interfaced with an Easy-nLC1200 liquid chromatography system (both Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 µm x 2 cm ...
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bioRxiv - Microbiology 2024Quote: The fractions were analyzed on an Orbitrap Fusion Lumos Tribrid mass spectrometer equipped with a FAIMS Pro ion mobility system and interfaced with an Easy-nLC1200 liquid chromatography system (all Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm ...
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bioRxiv - Cell Biology 2024Quote: ... Liquid chromatography (LC)-coupled tandem MS/MS analysis was performed using a hybrid timed ion mobility-time of light mass spectrometer (timsTOF Pro 2, Bruker) coupled to a nanoflow chromatography system (Ultimate nanoSRLC, Thermo Fisher Scientific) equipped with a reversed phase-C18 column (Aurora Elite 250×0.075 mm ...
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bioRxiv - Cell Biology 2024Quote: Each fraction was analyzed on an Orbitrap Lumos Tribrid mass spectrometer equipped with a FAIMS Pro ion mobility system interfaced with an nLC 1200 liquid chromatography system (all from Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm × 2 cm ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...