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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Genetics 2019Quote: ... human COT-1 DNA (Invitrogen) and biotin-labeled mouse COT-1 or minor satellite DNA (a gift from Dr ...
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bioRxiv - Genetics 2021Quote: ... human COT-1 DNA (ThermoFisher) to detect HSA21 and biotin-labeled I-EGFP-I-loxP-3’HPRT (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Human Cot-1 DNA (Invitrogen), 3M NaAc and 100% cold EtOH ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 μL of RNaseOut™ Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with recombinant murine EGF (100 ng ml−1, Gibco), recombinant human R-spondin 1 (200 ng ml−1 ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with recombinant murine EGF (100 ng mL-1, Gibco), recombinant human R-spondin 1 (200 ng mL-1 ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1ẞ high sensitivity kit sold by Invitrogen. The sample for our ELISA consisted of cell culture media (referred to as sample hereafter ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: Human foreskin fibroblasts (HFFs) were routinely maintained in DMEM with high glucose (Gibco, Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... Human hepatocellular carcinoma (HepG2) cells were cultured in high glucose DMEM (Thermo-Fisher Scientific) and supplemented with 10% fetal bovine serum (Gemini Biosciences ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: Human foreskin fibroblasts (HFFs) were routinely maintained in DMEM with high glucose (Gibco, Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: IMR-90 human lung fibroblasts (Coriell Institute) were cultured in high-glucose DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... were coated at 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) for >4 hours at ambient temperature and blocked ≥1 hour with 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 2% HI-FBS and 1% penicillin-streptomycin (Gibco 15140-122). Cells were maintained in humidified incubators at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and 293T were cultured in high-glucose DMEM (HyClone) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, ThermoFisher Scientific), 100 U/ml penicillin-G ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS and 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Bioengineering 2023Quote: ... The collected sections were split in two groups for staining: 1) lectin (DyLight 649; 1:100; Thermo Fisher) and α-SMA (1:100 ...
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bioRxiv - Biophysics 2024Quote: ... random group assignment) in a final volume of 100 μL of 1:1 phosphate-buffered saline (PBS, Gibco) and matrigel (BD) ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Bioengineering 2023Quote: ... In the LipofectamineTM CRISPRmax group (ThermoFisher, #CMAX00003), 3μg of 0xNLS-SpyCas9-2xNLS protein (18 pmol ...
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bioRxiv - Biophysics 2019Quote: ... The size of the TIM22 complex was determined based on the mobility pattern of a native protein molecular weight standard (cat number: LC0725, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... the DNA-protein interaction was detected with a LightShiftTM Chemiluminescent electrophoretic mobility shift assay (EMSA) kit (Thermo Fisher Scientific, USA). The primers used for EMSA are listed in Table S2.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: A group of 4 slices was loaded with 20 μM OGB-1 (Life Technologies O6807) in oxygenated aCSF containing 0.04% (w/v ...
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bioRxiv - Physiology 2019Quote: ... AlexaFluor-tagged secondary antibodies (Life Technologies) were used to detect primary antibodies ...
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bioRxiv - Systems Biology 2020Quote: ... 4μM fluorescein-tagged peptide (#F1314, Invitrogen) was loaded in the left side of the set-up ...
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bioRxiv - Cell Biology 2020Quote: ... fluorochrome-tagged secondary antibodies (Life Technologies) were added to coverslips for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and fluorescently tagged phalloidin (Invitrogen #A34055) were included during the secondary antibody incubation step ...
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bioRxiv - Molecular Biology 2020Quote: ... All of the UMI-tagged template molecules were added to the reaction containing 2 U Platinum SuperFi DNA Polymerase High Fidelity (Thermo Fisher Scientific, USA), and a final concentration of 1X SuperFi buffer ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2023Quote: ... custom micropatterned glass coverslips presenting 500 µm groWTh areas (CYTOOCHIPS Arena 500A) were covered with 800µL of 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were grown in glass surfaces previously coated with 10μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...