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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Plant Biology 2021Quote: ... and GST-△F-box-AFF1 proteins were immobilized on GST beads (Glutathione Agarose; ThermoFisher). Immobilized agarose beads containing 2 μg GST ...
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bioRxiv - Cancer Biology 2023Quote: ... Data were normalized on TBP (TATA-Box Binding Protein) or GAPDH (Thermo Fisher Scientific). The relative quantity was determined using ΔΔCt by the CFX Maestro software (BioRad).
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were incubated overnight with Paired Box 7 (PAX 7) antibodies (Invitrogen, USA) in blocking solution at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Large-scale expression of Strep-tagged proteins for purification was conducted in Trichoplusia Ni High Five cells (Thermo Fischer Scientific) in SF-900 medium (Gibco). Cells at a density of 2×106 cells/ml were infected with 1.5% of amplified virus and incubated for 40 hours ...
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bioRxiv - Microbiology 2020Quote: Expression vectors for FixL and FixJ His6-tagged proteins were generated using the Gateway high-throughput recombinational cloning system (Invitrogen). The entire fixJ gene or fixL amino acids 329-851 (lacking transmembrane domains ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Plant Biology 2021Quote: ... The second group was treated with proteinase (1 ng μL-1 Proteinase K Thermo Fisher Scientific) and RNase (0.4 ng μl-1 RNase A ...
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bioRxiv - Microbiology 2021Quote: ... and fluorescence tagged goat anti-mouse IgG Alexafluor 594 (1:200, Invitrogen) secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... purified mouse myelin tagged with pHrodo Red succinimidyl ester (1:100, ThermoFisher) or isolated human synaptoneurosomes from AD brains (Tai et al. ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) secondary antibodies for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... 1 unit/μl RNaseOUT ™ Recombinant Ribonuclease Inhibitor (Invitrogen), and 20mM Tris-HCl/10% glycerol for 25 minutes in ice ...
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bioRxiv - Cell Biology 2021Quote: ... ISG15 recombinant rabbit monoclonal antibody (1□00) (Invitrogen, 703132), and collagen I (1□100 ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). The lysates were cleared by centrifugation at 200,000 ×g for 10 min at 4°C 3 times to remove the contamination of the lipid ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). 10% of the precipitates were analyzed by western blotting to check the protein immunoprecipitation efficiency ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Electrophoretic mobility shift assay (EMSA) analysis was performed using the Light Shift® Chemiluminescent EMSA Kit (Thermo Scientific). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...