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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with virus containing scrambled control or targeting MTR and Polybrene (8 ug/mL, Invitrogen). Cells were split after 48 h into standard RPMI-media (10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... high titer virus generation (V1) was then used to infect 1200 ml High Five™ cells (Invitrogen) for large scale protein production ...
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bioRxiv - Immunology 2020Quote: ... Virus stocks used in vivo were produced by two passages on Vero E6 cells in DMEM (Gibco) without FBS ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type mumps virus (MuVi/Utrecht.NLD/40.10; genotype G) was grown on Vero cells in DMEM (Gibco) with 2% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfection and virus infection: Expression constructs were transfected into cells using Lipofectamine 2000 Transfection Reagent (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells (ATCC) were cultured for virus production in Dulbecco’s Modified Eagle Medium: Nutrient Mixture (DMEM) (GIBCO) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Biochemistry 2023Quote: ... This virus mixture was used to infect 1.8–2.4 liters of High Five cells (Thermo Fisher Scientific) at a cell density of 3 × 106 per ml for 60 h at 27°C ...
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bioRxiv - Microbiology 2023Quote: ... Infectious virus was reconstituted by transfecting ARPE-19 cells with BAC DNA and Lipofectamine 3000 (ThermoFisher L3000001); high titer stocks generated after three passages in ARPE-19 cells were used for this project ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed twice with PBS and then incubated with virus suspended in infectious medium (DMEM; Invitrogen) supplemented with 35% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... cells were infected with virus diluted in 10 mL of Opti-MEM (ThermoFisher Scientific, Waltham, MA, USA) and incubated for 1 h at 37°C to allow for virus adsorption ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells used for the production of virus were cultured in DMEM supplemented with 2mM glutamine (Gibco), 10% Tetracycline-free FBS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2019Quote: ... ZHBTc4 cells were transduced with pLV-pCAGGS-Tir1-V5 and PLEX-mCherry-OCT4-AID packaged lentiviral vectors (i.e Tir1-V5 and mCherry-Oct4-AID virus, respectively) and were selected with 2 μg/ml Puromycin (Thermo Fisher Scientific #A1113803) for 10 days ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then initially stained with microtubule-associated protein 2 (MAP2) monoclonal primary antibody (M13) (ThermoFisher Scientific,ON, Canada) at a 1:300 dilution then incubated overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentiviruses were obtained by transient transfection of the psPAX2 packaging plasmid and pMD2.G envelope plasmid in HEK293T cells by using Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA). Transfection efficiency was validated by western blotting ...
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bioRxiv - Neuroscience 2019Quote: ... fly brains or imaginal discs were promptly dissected in Schneider’s insect medium (Life-Gibco), properly mounted and scanned ...
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bioRxiv - Molecular Biology 2019Quote: ... larval brains were dissected and placed in insect media (HyClone SFX; Thermo Fisher Scientific) supplemented with 0.1 mM colchicine for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... RNA extraction from individual insects included homogenization with 1 mL of Trizol reagent (Ambion) and further purification using RNeasy kits (QIAGEN ...
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bioRxiv - Developmental Biology 2022Quote: ... Flies were dissected in Grace’s Insect Medium (Cat. No. 11605045; Invitrogen, Carlsbad, California, USA), and then fixed in 4% formaldehyde (Cat ...
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bioRxiv - Microbiology 2023Quote: ... Rasgon (The Pennsylvania State University) and were grown in Schneiderʹs insect medium (Gibco 21720024) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... braziliensis parasites (strain MHOM/BR/01/BA788) were grown in Schneider’s insect medium (GIBCO) supplemented with 20% heat-inactivated FBS ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 2 hours incubation with Protein A dynabeads (Invitrogen). Immunoprecipitated material was sequentially washed with buffer containing low salt (150 mM NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to nitrocellulose membranes using iBlot 2 (Invitrogen) and immunoblotting was performed as previously described in the western blot section.
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bioRxiv - Neuroscience 2019Quote: ... protein concentration was measured using Qubit 2 Fluorometer (Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 S1 protein primary antibody (Thermo Fisher Scientific) at 1µg/ml were added for 1 h at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... Patient KS1-1 was consented for stem cell derivation and cells were reprogrammed using non-integrating Sendai virus vectors (CytoTune-iPS 2.0, ThermoFisher Scientific, Waltham, MA). 5 days post-induction ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... pSPAX2 packaging vector and pVSVG envelope vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) as described (73) ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Immunology 2019Quote: ... Ka539 cells transfected with a virus expressing luciferase reporter were cultured in DMEM/high glucose and IMDM (Gibco) supplemented with 10% FBS (Natocor) ...
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bioRxiv - Microbiology 2020Quote: ... The cell layers were fixed on-chip and subjected to immunofluorescence microscopic analysis for influenza virus NP (Invitrogen) and neutrophils (CD45 ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated to passage three on Vero E6 cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (10,000 IU/mL ...
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bioRxiv - Microbiology 2019Quote: FVG-R infections were performed by inoculating A549 cells with virus diluted in Opti-MEM I (Invitrogen, 31985070) supplemented with 0.2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Cancer Biology 2023Quote: ... Epstein–Barr virus-transformed lymphoblasts were obtained from Coriell Cell Repositories and were grown in RPMI 1640 (Gibco) supplemented with 10% FBS and penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
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bioRxiv - Biochemistry 2023Quote: ... 8 ml P4 virus was used to infect 800 ml cultures of Expi293F GnTI-Cells (Thermo Fisher Scientific) cells at 2-3 × 106 cells supplemented with 1% Fetal Bovine Serum (FBS) ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Genomics 2024Quote: ... Virus was produced by transfecting HEK293T cells with the plasmid and the Lipofectamine 3000 kit (Thermo Fisher Scientific). U87MG were transduced with the supernatant containing the NY-ESO-1 virus ...
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bioRxiv - Microbiology 2024Quote: ... and CC81 (feline cell line transformed by mouse sarcoma virus ECACC 90031403) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-high glucose (GlutaMAX, Gibco) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Molecular Biology 2022Quote: ... Phasi Charoen like virus and Culex Y virus) were maintained at 28 °C in Leibovitz’s L-15 medium (Invitrogen: catalogue number: 21083027) supplemented with 10% foetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was detected by virus-specific primer/probe according to indicated reference sequences (Table 1) utilizing TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems) and QuantStudio™ 5 (ThermoScientific ...