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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Ex vivo lung slices were washed 3 × 30 minutes in PBS+0.5% Triton X-100) before incubating overnight at 4°C overnight with: 1:100 Alexa Fluor 488 goat anti-rabbit (Thermo Scientific - A11008) or anti-mouse (A32723 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained with primary antibodies overnight at 4°C targeting the following proteins: MAP2 (PA1-10005, ThermoFisher Scientific, 1:5000), AT8 (MN1020 ...
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bioRxiv - Biochemistry 2023Quote: ... ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher, 4:1 beads/cell).
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was cleared at 45’000⍰g for 25⍰min at 4⍰°C and the supernatant was loaded into HisPur NiNTA Chromatography 1 ml cartridge (Thermo Fisher Scientific). The resin was washed with 50 ml of buffer A containing 20⍰mM imidazole ...
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bioRxiv - Developmental Biology 2023Quote: ... mucus-like compounds were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBSt; Molecular Probes #L32460).
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bioRxiv - Immunology 2023Quote: ... a liver burn injury of approximately 1 mm3 was made on which a droplet of pHrodo Red succinimidyl ester (4 μM; Thermo Fisher Scientific) was administered ...
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bioRxiv - Immunology 2023Quote: ... The sections were fixed for 1 hour with 4% paraformaldehyde (PFA) in Hank’s Balanced Salt Solution (HBSS, Gibco, Waltham, MA, pH 7.2) supplemented with 0.1% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed and fixed with 4% paraformaldehyde in PBS for 10 min at room temperature then stained with Alexa Fluor 488 Phalloidin (1:500; Invitrogen cat# A12379) in blocking buffer for 20 min at room temperature and mounted in DuoLink PLA mounting medium with DAPI ...
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bioRxiv - Biochemistry 2024Quote: ... HBMEC were incubated overnight at 4°C with the primary antibodies (anti-β-catenin, # 71-2700; anti-ZO-1, # 61-7300; anti-CAT1, # PA5-82800, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 3x 10 min with PBS and incubated either for 2 hrs at RT or 24-72 hrs at 4° C in goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, ThermoFisher Scientific) secondary antibody in carrier solution containing 1% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with 5% non-fat dry milk overnight at 4°C under gentle agitation followed by 1,5 hours incubation at room temperature with anti-CRM1 (MA5-27879, Invitrogen, 1:2000 dilution) antibody to detect PmXPO1 protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed in PBS three times for 5 min at 4 °C by rocking and then incubated by rocking with secondary antibody (Goat-anti-Mouse 488, 1:2000, Invitrogen #A-11008) in 1% BSA PBST for 1 hour at RT in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was blotted for 3s (blot force 1) at 100 % humidity and 4°C in a Vitrobot Mark IV (Thermo Fisher Scientific) before plunge-freezing in liquid ethane.
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2023Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) i n PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 5 ul of cDNA reaction was used in each of 4 x 50 ul Round 1 PCRs per sample using Phusion polymerase in HF buffer (Life Technologies-F531L). The 4 Round 1 PCRs were then pooled and 5 ul of that mix were used as template for a 50 ul Round 2 PCR ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... The PVDF membrane was blocked for 1 h with 5% BSA in TBST buffer containing 0.1% Tween 20 followed by overnight incubation at 4 °C with primary antibody for: ERα (MA5-14104, dilution 1:400, Thermo Fisher Scientific) and PR (sc-7208 ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes isolated from human PBMCs (1 x 106 cells/well) were incubated with 27D6 antibody or isotype-matched control antibody (4 μg/mL, IgM, Thermo Fisher Scientific) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 ng of RNA were mixed with 4 µl of diluted ERCC RNA Spike-In Mix (Invitrogen, cat# 4456740. 1:1,000 dilution). All libraries were sequenced on the Illumina NovaSeq 6000 system.
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bioRxiv - Cancer Biology 2024Quote: ... the following secondary antibody was added overnight at 4°C in blocking buffer: Alexa FluorTM 647 donkey anti-rabbit IgG (1:1000, Invitrogen #A-32795). Finally ...
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bioRxiv - Immunology 2024Quote: ... 67 or Bacillus anthracis were loaded to 1-well 4-12% Bis-Tris NuPAGE Novex SDS-PAGE gels (Invitrogen, Thermo Fisher Scientific). Proteins were separated for 45 min at 200 V ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: To confirm protein enrichment on the pulldown samples the protein were eluted from the beads as mentioned above and 1/3rd of the eluted protein was resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and transferred onto transferred onto iBlot 2 Transfer Stack containing nitrocellulose membrane using the BOLT transfer system ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were separated by SDS-PAGE ((4-20%; Criterion XT precast gel) with 1× MOPS SDS gel running buffer (NP0001, Invitrogen, Life Technologies Corp). Following electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256 ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Microbiology 2020Quote: ... 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene) (Invitrogen D3922) in 200 mM HEPES buffer ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, Thermo Fisher Scientific Inc.) and blotted onto nitrocellulose (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, Thermo Fisher Scientific Inc.) and blotted onto nitrocellulose (926-31092 ...
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bioRxiv - Biophysics 2020Quote: ... the cell medium was changed to 4°C cold Leibovitz’s L-15 CO2 independent medium (Gibco). Prior to microinjection ...
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bioRxiv - Cancer Biology 2021Quote: ... submerged in 10 mL of freshly prepared 4% PFA in 1X PBS (Thermo Fisher Scientific, #28906), and placed on a rocker set at low speed for 24 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 °C for 10 minutes and re-suspended in 10 μL nuclease free water (Ambion, AM9937). Nuclei were then counted using a haemocytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was measured with Calcein AM live cell staining dye (4 mM stock from Invitrogen), which stains viable cells with green fluorescence ...
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bioRxiv - Immunology 2021Quote: After 4 h of treatment BoMac cells were recovered in 500μl of TRIzol (Invitrogen, Carlsbad, CA) and equal volumes of chloroform were added ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: Final cDNA amplified library concentration and quantity was determined using a Qubit 4 Fluorometer (Invitrogen Technologies) and 2100 BioAnalyzer (Agilent) ...
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bioRxiv - Cell Biology 2020Quote: ... samples were resolved on 4–20% polyacrylamide gels (15-well, Invitrogen; 26-well, Bio-Rad Laboratories), and transferred to PVDF (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... slices were transferred from the recording chamber to 4% paraformaldehyde in phosphate buffered solution (PBS, Affymetrix) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stimulated with NE / control treatments for 4 hours before being harvested using TRizol (Invitrogen) and were stored on −80° C until analyses were performed ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were subsequently incubated for 2 hours at 4°C with Streptavidin Dynabeads (Invitrogen M-280), and beads were successively washed with RIPA (2 times) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was performed on BOLTTM Bis-Tris Pure 4-12% gradient gels (Thermo Fisher Scientific) in MES-SDS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... we flowed through the chamber 4 mL of SYTOX Deep Red Nucleic Acid Stain (Invitrogen, S11380) at a 1:2000 concentration in DPBS with 0.1% v/v Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... V5-DNAJB6b-F93L-H31Q were cultured in DMEM containing 4 mM l-glutamine (Invitrogen; 11965-084), 10% FBS (Atlanta Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and horseradish peroxidase (HRP)-conjugated secondary antibodies (ThermoFisher) were incubated at RT for 1 hr ...