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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... hMOs were then washed overnight with 1X PBS at 4°C and incubated with Hoechst 33342 solution in 1X PBS for 2 h (1:1000, Thermo Fisher Scientific) and washed in PBS for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was refreshed every day and cells were passaged every 4-5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... 48 hours after transfection cells were splitted in a ratio of 1:4 and 300 μg/ml Hygromycin B (Thermo Fisher Scientific) was added to the cell culture medium for antibiotic selection of stably transfected cells ...
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bioRxiv - Physiology 2020Quote: ... containing 1.5 ml of chilled (4°C) transfer media composed of Ham’s F-10 medium including 1 mM L-glutamine (LG; Thermo Fisher Scientific, Denmark), 0.1% heat inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was incubated for 1 hr at 4°C with 200 µL of pre-washed magnetic Dynabeads M-270 Epoxy (Thermo Fisher Scientific) conjugated to rabbit IgG (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... each of the libraries was diluted 4-fold prior to running on a 1% Agarose E-gel (Life Technologies, #G402001, Carlsbad, CA) with a E-Gel 1-Kb Plus DNA Ladder (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... mucus-like compounds in Xenopus were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBST; Molecular Probes #L32460). Detailed protocol was published in (Walentek ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 5% (w/v) skimmed milk overnight at 4°C and probed with anti-DsbA (1:1000, Thermo Fisher Scientific) or anti-AP (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in TBS containing 0.2% Triton X-100 three times and incubated in Alexa 488-conjugated goat anti-rabbit secondary antibody (1:500, 4 hours; Invitrogen, A-11034) containing bisbenzimide (1 μg/ml) ...
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bioRxiv - Biophysics 2021Quote: ... we diluted the provided solution 1:100000 and added 4 μL of the dilution to a coverslip (#1.5, Thermo Scientific Menzel Gläser). We then used a second coverslip used on top of the first one to have homogeneous distribution of beads on the field of view ...
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bioRxiv - Biophysics 2021Quote: ... Cells were induced with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours and harvested by centrifugation (Sorvall LYNX 6000, ThermoFisher Scientific) at 8,000 rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C followed by washing and incubation with alexa-conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-3 hour in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 10 min in PBS 1X + 0.1% Triton X-100 + 1% BSA and incubated for 6h with secondary antibodies at 4°C: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell counts are re-determined by using the Countess ll FL and adjusted to ~1000□cells/μl by low speed centrifugation at 4□°C and re-suspended in 1× PBS without calcium or magnesium (Thermo Fisher MT21040CV) supplemented with 0.04% BSA to prepare cells for emulsification ...
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bioRxiv - Biochemistry 2022Quote: ... The MTS were stained with a mixture of 2 μM calcein AM and 4 μM ethidium homodimer-1 in PBS (Thermo Fisher Scientific) to stain for live and dead cells ...
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bioRxiv - Microbiology 2022Quote: ... GTX135357) primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, Carlsbad, CA, USA) secondary antibodies for 40 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... cells were washed three times with ice-cold PBS and then incubated for 30 min at 4°C in PBS containing 1 mg/ml EZ-Link-sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific). Next ...
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bioRxiv - Immunology 2020Quote: ... Approximately 5 mg of purified ACE2 PD was covalently attached per 1 mL of 4% beaded agarose by amine coupling using AminoLink Plus resin (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... The cells were washed twice and then reacted for 30 min at 4°C with Streptavadin-Cy5 (Thermo Fisher; 1:1000 dilution) or anti-mouse IgG-Alexa488 ...
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bioRxiv - Microbiology 2021Quote: One-tenth of the protein samples and their corresponding inputs (pre-diluted 1/10) were size-separated on a bolt 4-12% Bis-Tris plus (Thermo Fisher Scientific) and stained with the silver stain kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were collected by spinning at 1000g for 5 min at 4°C and cell pellets were resuspended in 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... microsphere mixtures were successively incubated with serum samples (1:400) biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific), and Streptavidin-R-Phycoerythrin (4 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 20 min at room temperature and stained overnight at 4 °C using an anti-V5 antibody (1/500; Invitrogen, Cat R96025) or anti-FLAG antibody (1:100 ...
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bioRxiv - Bioengineering 2020Quote: ... samples were fixed with 4% paraformaldehyde (w/v) in PBS for 1 h and stained with Actin Red 555 (Molecular Probes, ThermoFisher) for 1h ...
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bioRxiv - Neuroscience 2022Quote: ... Incubations were performed for 14h at 4° C followed by incubation with secondary antibodies: Alexa 568 anti-mouse 1:200 (Thermo Fisher A10037), and Alexa 488 anti-goat 1:200 (Thermo Fisher A11055 ...
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bioRxiv - Genomics 2022Quote: ... Human cell lysate and mouse cell lysate (as a spike-in internal control) were combined (∼4:1 ratio) and incubated with protein A or G Dynabeads (Thermo Fisher Scientific) conjugated with the relevant antibodies for 14 h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... culture dishes were coated at 4 °C overnight with collagen I at a 1:8 concentration diluted in ddH2O (Gibco, #A10483-01). The next day ...
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bioRxiv - Biochemistry 2022Quote: 400 μL of the total cleared lysates were treated with 4 μL of DNase I (1 U/μL, EN0525, Thermo Fisher Scientific) and 8 μL of RNase I (100 U/μL ...
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bioRxiv - Cell Biology 2022Quote: ... 45 s in air at 15 mA and 0.4 mbar) holey carbon grid (C-flat: 2/1-3C-T) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) after blotting for 3 seconds with blot force 18 ...
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bioRxiv - Molecular Biology 2019Quote: ... Red blood cell cultures (~4% hematocrit) were crosslinked for 10 min by direct addition of methanol-free formaldehyde (1% final concentration) (Thermo Fisher # 28908). The cross-linking reaction was quenched using a final concentration of 0.125 M Glycine for an additional 5 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila antisera (1:20,000) for 1h at 37°C in PBS containing 4% BSA followed by anti-rabbit IgG Alex Fluor 488 (Invitrogen, 1:500) for 1h at 37°C in PBS containing 4% BSA ...
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bioRxiv - Genetics 2019Quote: ... Each sample containing equal amount of proteins was incubated overnight at 4 ℃ with mouse anti-V5 (1:500; Thermo Scientific, R960-25). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were blocked with 4% goat serum in PBS for 1 hr and then incubated with primary antibodies against cTnT (Thermo Fisher Scientific), α-actinin (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... The cells were washed twice and then reacted for 30 min at 4°C with Streptavadin-Cy5 (Thermo Fisher; 1:1000 dilution) in PBS containing 0.5% BSA, ...
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bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
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bioRxiv - Zoology 2021Quote: ... Larvae were subsequently incubated for 12-24 h at 4°C in Alexa Fluor®488 goat anti-rabbit (1:1000 dilution, Molecular Probes). After incubation in the secondary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... mESCs were co-transfected with 4 μg of transposable vector and 1 μg of the piggyBac transposase using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... were used to amplify a 323 bp region of exon 1 of the CD44 gene spanning the cut site for 4 cycles using Phusion Hot Start II DNA Polymerase (Thermo Fisher Scientific). PCR products were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, Invitrogen, USA; 1:500 dilution) and AF488-conjugated α-bungarotoxin (B13422 ...
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bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, ThermoFisher, USA; 1:500 dilution), Cy5 goat anti-rabbit IgG (H&L ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) and rabbit anti-mKO polyclonal antibody (1:500 ...
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bioRxiv - Pathology 2021Quote: ... 1:1000) at 4□ overnight followed by fluorescent staining with Alexa Fluor Plus 647-conjugated donkey anti-rabbit IgG (A32795, Invitrogen, 1:1000) and Alexa Fluor® Plus 555-conjugated donkey anti-mouse antibody (A32773 ...
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bioRxiv - Immunology 2021Quote: ... S2P6 was serially diluted 1:4 starting at 80 μg/ml in infection media (DMEM supplemented with 2% FBS and 20mM HEPES (Gibco, 15630-080)) and incubated with replication-competent VSV-SARS-CoV-2 (23 ...