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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution: Alexa 488-goat anti-rabbit (1:1000; Thermo Fisher Scientific) and Alexa 594-donkey anti-mouse (1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the brains were rinsed in PBS for 4 × 15 min before being incubated in the Cy2-conjugated anti-mouse secondary antibody (dilution 1:300 in PBSX; Invitrogen, Eugene, OR), at 4 C for 1 d ...
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bioRxiv - Immunology 2020Quote: ... PBMC were collected and washed twice (400 × g, 8 min, 4°C) with cold PBS containing 1 mM UltraPureTM EDTA (Invitrogen, ThermoFisher, Basel Switzerland). In order to remove platelets ...
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bioRxiv - Microbiology 2020Quote: ... J774A.1 macrophages were seeded at 4×105 macrophages/well in RPMI with 10% FBS in sterile 16-chamber slides (Nunc Int., Denmark). After incubation ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBS and incubated for 2 h at 4°C with appropriate combinations of AlexaFluor-conjugated secondary antibodies (Invitrogen,1:2000) for 2 hours at 4C protected from light ...
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bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody incubation was also performed overnight at 4°C with donkey anti-mouse Alexa 647 (1:250, Invitrogen, Cat. # A-31571).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C in a primary antibody/0.5% BSA/PBS-T solution of rabbit anti-TH (1:1000, Invitrogen, Carlsbad, CA, 701949). Sections were then washed 6×10 minutes in PBS-T before incubating in secondary antibody/0.5% BSA/PBS-T solution of Alexa568 donkey anti-rabbit (1:2000 ...
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed with PBS and lysed at 4°C in ice-cold 1% triton lysis buffer supplemented with protease inhibitors (ThermoFisher Scientific, A32963) and phosphatase inhibitors (5 nM Na3VO4 ...
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bioRxiv - Cell Biology 2022Quote: ... Treated cells were incubated for 1 and 4 hours and total protein was isolated using RIPA lysis and extraction buffer (Thermo Scientific, 89900) and run using SDS-PAGE in 10% precast polyacrylamide gels (BioRad ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were then washed in PBS with 2% Triton X-100 at 37°C for 4 days and incubated with goat anti-rabbit Alexa 633 (A-21071, Invitrogen, 1:100) at 37°C for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... The leaflets were then incubated for 2 days in the dark at 4°C with goat anti-Rat IgG Cross-adsorbed Alexafluor 594 secondary antibody dilution 1:500 (#A11007, Thermo Fisher Scientific), washed 3 times in 1 mL PBS (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: First-round PCR amplification of exon 4 was carried out on 1 μl of single worm lysate using Phusion Green High-Fidelity DNA Polymerase (Thermo Scientific, F534S) in a final volume of 20 μl as follows ...
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bioRxiv - Microbiology 2022Quote: ... proteins were separated on Bolt 4-12% Bis-Tris gel in 1 × Bolt MOPS SDS running buffer (Thermo Fisher Scientific catalog # B0001). Separated proteins were electro-transferred to nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were centrifuged at 300 x g for 5 min at 4°C and treated with 1 mL of Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Gibco; cat#A1049201). After 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4 °C with the primary antibody rabbit anti-CaMKIIα polyclonal antibody (1:1000, Invitrogen PA5-84083), mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were processed for 48 hours at 4 degrees with the primary antibody in blocking solution (1:1250, rabbit anti-Esr1, Invitrogen PA1-309), rinsed 3×10 minutes in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4°C in a 1:500 dilution of primary antibody: Tyrosine Hydroxylase (TH, mouse, Invitrogen; MA1-24654) and the immediate early gene protein c-Fos (c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed cultures were washed twice in 0.01 M PBS and incubated overnight at 4 °C with donkey anti-rabbit Alexa488-labeled secondary antibody (1:1000; Invitrogen, Cat# A21206) in 0.01 M PBS with 10% NGS ...
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bioRxiv - Neuroscience 2022Quote: Fixed slices containing biocytin-filled neurons were washed with PBS 4 x for 10 minutes and transferred to a solution containing AlexaFluor Streptavidin 647 (Invitrogen, 1:500) and Neurotrace 435/55 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at 4°C overnight and then blocked for 1 hour at 37°C using SuperBlock (ThermoFisher Scientific, Cat. # 37516), and then washed four times with PBS 0.05% Tween-20 (PBST) ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4 °C with the primary antibody (anti-TMEM173/STING antibody (OT14H1),1:100 dilution) (Invitrogen, MA,USA). After they were rinsed with PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged with a ratio of 1:4 to when they reached 70% to 80% confluency by incubation cells with 0.5 mM EDTA (Fisher Scientific MT-46034CI) at room temperature for 5 minutes.
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bioRxiv - Microbiology 2024Quote: ... Fixed monola were blocked with 4% BSA-PBST and probed with primary antibodies for pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:250 dilution) and Chlamydia heat-shock protein 60 (cHsp60 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with 4% horse serum in PBS for 1 hour and then incubated with primary antibodies against cTnT (Thermo Fisher Scientific), α-actinin (Abcam) ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Microbiology 2024Quote: ... and 4 μl was spotted onto LB agar or on LB agar with 1% (w/v) sodium deoxycholate (DOC, Thermo Fisher Scientific), as has been previously described (56) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min with PBS and incubated over-night at +4°C with goat anti-rabbit Alexa Fluor 564 (1:1000, Invitrogen, ThermoFisher Scientific) and goat anti-mouse Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 7 min with PBS and incubated overnight at +4°C with goat anti-rabbit Alexa Fluor 594 and goat anti-mouse Alexa Fluor 488 (each 1:1000, Invitrogen, ThermoFisher Scientific) in carrier solution ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were resuspended in 10 µL of water and labeled for 1 h at room temperature with 4 µL (∼11 µg/µL in acetonitrile) of TMT10plex or TMT18plex (Thermo Fisher Scientific). The reaction was quenched with 4 µL of 5% hydroxylamine ...
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bioRxiv - Neuroscience 2024Quote: ... Cellular RNAs captured by bead-bound antibody complexes were digested to <100 nt by incubating for 30 min at 4°C with RNase I (1 U/μl; Thermo Fisher Scientific Ambion; AM2294). After extensive washing ...
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bioRxiv - Neuroscience 2024Quote: ... the slices containing the fixed neurons cross-linked with 4% paraformaldehyde and stained with streptavidin Alexa Fluor 555 conjugate (Invitrogen, 1:1000). Fluorescent images were obtained using a confocal microscope (ZEISS LSM 900 ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cancer Biology 2022Quote: Approximately 5 × 106 MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h) were fixed with 1% methanol-free formaldehyde solution (Thermo Scientific, #28908) for 10 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were pre-extracted with 0.1% Tween-20 in PBS (PBST) for 1 min and then fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were co-transfected at ∼80% confluence with wild-type (4 μg) or mutant SCN1A construct (4 μg) and enhanced green fluorescent protein (eGFP (1 μg) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) and incubated under the culture conditions above for 24 hours ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Biochemistry 2023Quote: ... then resuspended in 100 µL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... for two hours at room temperature Sections were incubated in the following primary antibodies overnight at 4 °C: ERβ (rabbit, 1:200, Invitrogen PA1-311) and NeuN (mouse ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT immortalized BJ-5ta foreskin fibroblasts were cultured in a 4:1 mixture of DMEM medium and Medium 199 (Gibco, Life Technologies) supplemented with 10% FBS ...