Labshake search
Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... plates were washed six times with PBST and developed by adding 50 μL of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific 34022) per well ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed 3 times with WB before adding 100 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher 34029) followed by incubation in the dark at room temperature with shaking (225 rpm ...
-
bioRxiv - Bioengineering 2022Quote: ... Plates were finally washed three more times with PBS-T and developed using 1-Step Ultra TMB-ELISA solution (Thermo Fisher Scientific #34028). The HRP reaction was stopped with 1M hydrochloric acid and absorbance values at 450 nm were measured on a SpectraMax i3x plate reader (Molecular Devices).
-
bioRxiv - Biochemistry 2023Quote: ... three times washed with 1%BSA (prepared in 1XTBS) followed by development of signal by treating cells with 200μL TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue color appeared ...
-
bioRxiv - Neuroscience 2021Quote: ... SP was labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
-
bioRxiv - Genetics 2022Quote: ... The pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Fisher Scientific, 78840) or resuspended in 1x RIPA buffer with protease inhibitors to extract whole cell lysate.
-
bioRxiv - Cell Biology 2019Quote: ... The cell pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Scientific, 78840), or resuspended in 1 x RIPA buffer with protease inhibitors to extract whole cell lysate ...
-
bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
-
bioRxiv - Neuroscience 2022Quote: ... and nuclear compartments using consecutive centrifugation steps of varying speeds and specialized buffers obtained from a commercially available kit (Sub-Cellular Protein Fractionation Kit for Tissues, ThermoFisher). Bradford protein assays were performed for each compartment individually ...
-
bioRxiv - Microbiology 2023Quote: The protein content of pEVs were quantified using Pierce BCA kit according to the manufacturer’s instructions (BCA Protein Assay Kit, Thermo Fisher).
-
bioRxiv - Cancer Biology 2021Quote: ... Protein extracts or purified sEVs were quantified for protein content using the bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific #23225). Equal amounts of cell lysate or purified sEVs were resolved by SDSPAGE and transferred into a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Bioengineering 2022Quote: ... Lysates were clarified by centrifugation (20,000 × g, 15 min) and protein concentration was determined (BCA protein assay Kit, Pierce®, Thermo scientific, IL, USA). The same protein amount (usually 20-30 μg ...
-
bioRxiv - Immunology 2021Quote: ... 10 min) and cell-free supernatants were snap-frozen for total protein analysis using the Pierce™ BCA protein assay kit (Thermo Scientific; #23227). The pellet was resuspended with 400 μL of PBS and total leukocyte count was measured via a hemocytometer (Neubauer chamber) ...
-
bioRxiv - Genomics 2020Quote: ... SDS) for 10 minutes at 95°C and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Protein samples were separated by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ gels (BioRad ...
-
bioRxiv - Bioengineering 2020Quote: ... The purified protein was fluorescently labeled with Alex Fluor™ 568 and 647 protein-labeling kits (Thermo Fisher Scientific Cat# A10238 and A30009) to produce hmAb-AF568 and hmAb-AF647 following the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2021Quote: ... 5 ×105 cells with or without transduction and their supernatants were harvested with RIPA buffer and the protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Equal amounts of protein (50 µg ...
-
bioRxiv - Bioengineering 2021Quote: ... The total protein concentration of the EVs was measured using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) to estimate the concentration and the yield ...
-
bioRxiv - Neuroscience 2019Quote: ... The supernatant was collected from the samples and BCA Assay was performed to determine the protein content (Pierce™ BCA Protein Assay Kit, Thermo Scientific, 23223).
-
bioRxiv - Developmental Biology 2021Quote: ... The supernatants were transferred into new tubes and protein concentrations were quantified using the Pierce BCA Assay Protein kit (Thermo Fisher Scientific – 23225). 60μg total protein were mixed with SDS gel loading buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The results were normalized using total protein content of the cells that was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Experiments have been performed in triplicates in five independent experiments.
-
bioRxiv - Microbiology 2020Quote: ... sera were harvested and IgGs were purified on protein A and labeled with Alexa Fluor™ 647 using the protein labeling kit (Thermo Fisher Scientific) as recommended by the manufacturer.
-
bioRxiv - Cell Biology 2020Quote: ... the total protein concentration was quantified and normalized in all samples using the Pierce BCA protein assay kit (ThermoFisher Scientific, Waltham, MA, USA). Results were expressed as median fluorescence intensity (MFI).
-
bioRxiv - Neuroscience 2022Quote: ... v/v) before LC-MS/MS analysis according to the total protein content (quantified using BCA protein Assay Kit (Thermo Scientific, Massachusetts, USA).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the supernatant was collected and the cleared lysate protein concentration was quantified using the BCA protein assay kit (Thermo Scientific, Pierce Rockford, IL) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... Total protein concentration in lysates (supernatants) was determined using the BCA method (Pierce™ BSA Protein Assay Kit, Thermo Fisher Scientific, Waltham, MA). Proteins (20-30 μg ...
-
bioRxiv - Molecular Biology 2022Quote: ... soluble nuclear and chromatin-bound protein extracts were prepared by stepwise separation using Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific, 87790). For this purpose ...
-
bioRxiv - Biochemistry 2022Quote: ... combined protein concentrations of complex I and MSP2N2) were determined using the Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific). For the sample subjected to cryo-EM analyses ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). Lysates containing 5 μg of total protein in Laemmli sample buffer were loaded onto a 6% (v/v ...
-
bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a fresh tube and protein was quantified using a Pierce BCA Protein assay kit (Thermo Fisher; Cat. #23225).
-
bioRxiv - Microbiology 2020Quote: ... Concentration of the final purified protein solution was quantified using the Pierce™ Bradford Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
-
bioRxiv - Developmental Biology 2022Quote: ... The supernatant from each tube was isolated and protein concentration for each sample was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL). 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad ...
-
bioRxiv - Genomics 2020Quote: ... cell debris was removed by centrifugation at 10000rpm for 10 min and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were prepared for SDS-PAGE (30μg loaded per well) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Amylase levels were normalized to the total protein content determined by a BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, 23225).
-
bioRxiv - Immunology 2019Quote: Ab019 and Ab161 were purified by incubation with Protein G beads and their concentrations were determined by BCA protein assay kit (Thermo Scientific, IL, USA). According to the manufacturer’s manual ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant was transferred into a fresh tube and protein concentration determined using the Pierce™ BCA protein assay kit (Thermo Scientific, #23225). 550ug protein from each sample was diluted to 500ul and mixed with 3 ug ACTN2 antibody (Abcam ...
-
bioRxiv - Cancer Biology 2019Quote: The EV fraction or the transfected PCa cells was measured for protein content using the Micro BCA Protein Assay kit (Thermo Scientific, Wilmington, DE). Equal amounts of protein were loaded onto 4-20% Mini-PROTEAN TGX gels (Bio-Rad ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein concentration was determined for each sample using a Pierce reducing agent-compatible BCA protein assay kit (Thermo Fisher Scientific, cat. no. 23250).
-
bioRxiv - Neuroscience 2022Quote: ... for 10s/sample on ice and protein concentration was determined using Pierce BCA protein assay kit following manufacturer’s instruction (Thermo Fisher Scientific cat#23227). 20μg to 30 μg of total protein from each lysate was combined with loading buffer (4X ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). OCR measurements were normalized to the protein concentration in each well.
-
bioRxiv - Genomics 2022Quote: ... for 30 minutes at 4°C and supernatant was retained and protein concentration quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). A total of 25-35μg proteins were loaded onto 12.5% Tris-HCl gel for SDS-PAGE analysis ...
-
bioRxiv - Microbiology 2022Quote: ... Protein concentration of NAD+ or NADH extracts was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL) to normalize NAD(P)H and NAD(P)+ concentrations.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA). Activity was compared to the solvent control samples (set at 100% activity) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The whole cell lysate were obtained with RIPA and the protein concentration was measured using BCA protein assay kit (23225, Thermo Scientific, MA, USA). Monoclonal antibody anti-DNMT1 (ab188453 ...
-
bioRxiv - Microbiology 2022Quote: ... pH 8) was added on each pellet and total protein quantification was performed using BCA Protein Assay kit (Pierce™, Thermo Fisher Scientific). Before immunobloting analysis ...
-
bioRxiv - Microbiology 2022Quote: Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). The lysates were loaded in the amount of 5 µg of total protein in Laemmli sample buffer onto a 6% (v/v ...
-
bioRxiv - Neuroscience 2024Quote: Total protein levels of all CSF samples were determined by use of a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA). CSF Aβ40/Aβ42 and total tau (t-tau)/tau phosphorylated at threonine-181 (p-tau ...
-
bioRxiv - Physiology 2024Quote: ... and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit; #23225, Thermo Fisher Scientific, Rockford, IL).
-
bioRxiv - Microbiology 2024Quote: ... The membrane proteins of AGS cells were isolated with the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, United States). Briefly ...
-
bioRxiv - Immunology 2024Quote: ... The final spike protein concentration was determined by measuring absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Proteins were dispensed into 0.5 ml aliquots and stored at -80°C.
-
bioRxiv - Cell Biology 2023Quote: ... cells were lysed in Ripa Buffer and total protein was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227) for data normalization.