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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Anti-PDIA3 (AMAB90988; WB, 1:3000; IF, 1:500), RanGAP1 mouse (33-0800; IF, 1:300; PLA, 1:500) was from Invitrogen. Anti-VAPB (66191-1-1g ...
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bioRxiv - Systems Biology 2020Quote: ... For each step proteome was quantified using a commercially available assay kit (Micro BCA™ Protein Assay Kit, Thermofisher) and samples were diluted to be in the linear range of BSA protein standard range (0.5 to 20 μg/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane and cytosolic fractions were isolated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific kit #89842).
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was evaluated and 1 mg total protein was incubated with 30 μl streptavidin-magnetic beads (ThermoFisher, 88816) for 1 h at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... An equal amount (500µg) of each protein lysate was precleared for 1 hour with magnetic Protein G Dynabeads (Invitrogen) and immunopurified with 40µl washed anti-V5 agarose beads (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed by incubating in trypsin (1 µg trypsin/50 µg protein; Thermo Fisher Scientific, Waltham, MA) for 3 h ...
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bioRxiv - Genetics 2023Quote: Protein samples were prepared from 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified protein samples were incubated with NeutrAvidin® Agarose resin (50 µL per 1 mg protein, Thermo Scientific) for 2 hours at RT ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... followed by chloroform-methanol protein precipitation to obtain pure protein pellets that were subsequently resuspended in digestion buffer (100 mM TEAB with 1% (v/v) SDC) and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, United States). From each sample ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Final sEVs pellet was resuspended in 100 μL of PBS and the protein content was measured by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). The NS500 nanoparticle characterization system (NanoSight ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant mixture was centrifuged at 14,000 g and the supernatant was used to estimate the total protein by using Pierce™ BCA Protein Assay Kit (Thermo scientific). Equal quantity of protein (300 μg ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Cell Biology 2020Quote: ... Membrane pellets were gently resuspended in 100 μl SEM and protein content was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) to normalize total protein concentration across samples ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Cell Biology 2019Quote: ... anti-TAC102 antibody is crosslinked to protein A / protein G beads from the Pierce™ Crosslink Magnetic IP kit (Thermo Scientific™). A mitochondrial enriched fraction is prepared by digitonin fractionation with 100 ml of procyclic form parasites (cell line ...
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bioRxiv - Cell Biology 2020Quote: ... TAG amounts were normalized to the total protein amounts in each sample using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). The data were analyzed using a Student’s t test.
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bioRxiv - Microbiology 2020Quote: The proteins from cell lysates and isolated mitochondria were analysed by separating 10-40 μg of protein (concentrations determined using Pierce BCA Protein Assay kit, Thermo Fisher Scientific), by SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... the P2 fraction was brought up in 400uL of PBS and protein concentrations were measured using the QuBit Protein Assay Kit (Thermo Fisher, Q33211). Samples were precleared with 15mg of DEPC treated (to inactivate RNAse ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the isolated exosomes was detected by using a total exosome RNA and Protein Isolation Kit (Thermo Fisher Scientific) and a BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer instructions.
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... we transferred the supernatant to a clean tube and determine the protein concentration using the EZQ® Protein Quantitation Kit (Cat. no. R33200,Thermo Scientific) or another method ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We quantified the protein content in the clarified lysates of the damselflies using Pierce™ BCA protein assay kit (Thermo Fisher Scientific). We used the Bovine Serum Albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... Protein pellets from each aliquot per sample were pooled and the total protein content were measured using BCA protein Assay kit (ThermoFisher Scientific, Australia). The protein disulphide bonds were reduced with 10 mM dithiothreitol for 30 min at 60 °C and the cysteine residues were alkylated with 20 mM iodoacetamide for 30 min at 37 °C in the dark ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Protein content in each sample was measured with NanoOrange Protein Quantification Kits according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA), and was used for ATP synthesis rate normalization ...
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bioRxiv - Bioengineering 2019Quote: Proteins were collected 48 hours post-transfection and their concentrations were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Western blots were performed as described previously (Lee et al ...
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bioRxiv - Pathology 2019Quote: ... Protein concentration of the supernatant was determined using the BCA assay (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, cat. number 23225). Samples were mixed with SDS-loading buffer without heating and electrophoretically separated on a 4–20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extracts were diluted in PBS and quantified in duplicate using a Micro BCA Protein Assay Kit (ThermoFisher Scientific, cat no: 23235). Bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2020Quote: ... Intensity quantification was performed using ImageJ software and the obtained values were corrected by culture media background (without having been in contact with cells) and total protein content measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Values were further normalized by internal negative and positive controls from the antibody assay ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear membrane and debris were pelleted by centrifugation and protein concentration of the cleared lysate was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 2μg of the anti-BAF155 antibody (ab172638 ...
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bioRxiv - Biochemistry 2020Quote: ... The cell debris was removed by centrifugation and the protein concentration of the supernatants were determined using Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... followed by collection of supernatants for quantification of total protein concentration using Microplate BCA Protein Assay Kit (Pierce™, Thermo Fisher Scientific). Equal amounts of protein were separated by SDS-PAGE using 4-15% SDS polyacrylamide gels (Bio-Rad) ...
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bioRxiv - Bioengineering 2019Quote: ... The tissues were dissolved in RIPA buffer and the total protein concentration was measured with a Pierce BCA protein assay kit (Thermo Fisher Scientific). Proteins (15 μg each ...
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bioRxiv - Cell Biology 2020Quote: ... was solubilized with 0.1 N NaOH and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). Cellular cholesterol content data was expressed as mg cholesterol/mg of protein ...
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bioRxiv - Biophysics 2020Quote: ... For more accurate characterization protein concentration was determined by the bicinchoninic acid method31 with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). A method based on pyridine hemochrome spectra32 was used to determine the amount of heme ...
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bioRxiv - Biophysics 2020Quote: ... proteins were labeled using Alexa-fluor 488™(green) or Alexa-fluor 594™(red) microscale protein labeling kits (ThermoFisher Scientific, Invitrogen). Small amounts (∼0.3 µl ...
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bioRxiv - Biophysics 2020Quote: ... proteins were labeled using Alexa-fluor 488™(green) or Alexa-fluor 594™(red) microscale protein labeling kits (ThermoFisher Scientific, Invitrogen). Small amounts (∼0.3 µl ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of each lysate was measured using the BCA protein assay kit according to manufacturer’s directions (Thermo-Fisher Scientific, Waltham, MA). Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined using the Thermo Scientific™ Pierce™ BCA (bicinchoninic acid) Protein Assay Kit (Thermo Scientific™ Cat# 23225). Following concentration determination ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were passed through a 0.22 μm Spin-X filter column after which total protein amount was determine using the Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Equal protein amounts were denatured in SB and subjected to SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the tissue lysates were measured with the aid of a bicinchoninic acid protein assay kit (Thermo Scientific, Rockford, IL). TNF-α ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were transferred to new ice-cold Eppendorf tubes and protein concentration was measured by the BCA assay using Pierce BCA Protein Assay kit (ThermoFisher Scientific; #23225). Equal amounts of protein were loaded on 4-12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Bioengineering 2021Quote: ... Total protein quantification was performed in samples using Pierce™ BCA (Bicinchoninic Acid) Protein Assay Kit (Thermo Fisher Scientific Inc. Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... sonicated 3 three times for 10 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... the cleared supernatant was collected and the protein content in the soluble fraction was determined using the Pierce® BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s instructions ...