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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The final Spike protein concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Final proteins were dispensed in aliquots of 0.5 ml each and stored at -80°C prior to use.
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bioRxiv - Systems Biology 2019Quote: ... Protein pellets were dissolved in 200 uL 5% SDS solution and protein concentrations were determined using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific).
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bioRxiv - Plant Biology 2019Quote: ... The protein concentration of the clear supernatant was determined using the bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce).
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of the tissue homogenates or whole-cell extracts were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... The 3 eluted fractions were pooled and the protein concentrations measured with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, 23225) using the Pierce™ Bovine Gamma Globulin Standard (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... CLICK-IT enrichment of AHA-labelled secreted proteins was performed with the Click-iT™ protein enrichment kit (Thermo Fisher Scientific, C10416) as previously described67,68 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The protein content of supernatants was measured using a BiCinchoninic acid Assay (BCA) protein assay kit (Thremo Fisher Scientific, Mississauga, ON, Canada). Equal amounts of protein in lysis buffer (10 µg ...
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bioRxiv - Neuroscience 2021Quote: ... An input sample of 75 μl was removed from the lysate and protein concentration was assessed using a Pierce Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, 10678484). Concomitantly ...
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bioRxiv - Physiology 2021Quote: ... The total concentration of proteins was measured with the Pierce Rapid Gold BCA Protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA). The E- ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in ddH2O by freeze/thaw cycles and the protein concentration was determined using the Pierce™ BCA protein assay kit (ThermoFisher Scientific). Polyamines were extracted by Bligh and Dyer extraction (Noga et al. ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Developmental Biology 2022Quote: ... A loading control was performed by total protein staining using Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Fisher Scientific, 24580). For blocking ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein levels in the samples were assayed using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific; Cat. # 23227) and diluted with 500 mM TEAB so that the total protein level of each sample would be approximately the same ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein concentration in the supernatant was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA, USA). After diluting with loading buffer and boiling for 10 min at 95°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The total protein concentration for each culture was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Using the same amount of protein from each sample ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified from yeast supernatants by Ni-NTA resin affinity chromatography and protein concentration was determined using Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Expression levels of proteins of interest were normalised by total protein amount (Ghosh et al, 2014) (Moritz, 2017) using a Pierce reversible protein stain kit (#24580, Thermo Fisher Scientific). Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using the PierceTM BCA Protein Assay Kit (Cat# 23225, Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). Proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenates were centrifuged at 4 °C for 30 min at 9300 × g and total protein concentration in the supernatants was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and cytosolic and membrane proteins were extracted with the use of a Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842).
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bioRxiv - Biochemistry 2024Quote: ... and subcellular protein fractionation was performed on 1.0 x 107 cells using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific, Waltham, MA). Protein concentrations were determined using bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each lysate was measured using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). The 2′-3′-cGAMP content were measured by 2′-3′-cGAMP ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each sample was determined by the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). Equal amounts of proteins (up to 20 μg ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellet was resuspended in PBS/EDTA buffer and the protein concentration of the homogenate measured using the Pierce BCA protein assay kit (Thermo Scientific, USA). AChE activity was measured using the Ellman assay using acetylthiocholine as substrate and the indicator 5,5′-dithiobis-2-nitrobenzoic acid (DTNB ...
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) before being used for in vitro acetylation.
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified proteins was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) as described previously (Choi et al. ...
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bioRxiv - Cell Biology 2023Quote: ... was added as a supplement to the lysis buffer and the protein concentration was measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... protein concentrations were measured with the Pierce BCA protein assay kit coupled with the bovine serum albumin standards (23225, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Microbiology 2023Quote: ... calcitrans salivary proteins by size with proteins visualised by Coomassie brilliant blue dye or silver staining (Pierce™ Silver Stain Kit, ThermoFisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... the protein concentrations of synaptosome samples resuspended in HEPES buffer were tested by the Pierce BCA Protein Assay Kit (Thermo Fisher #23225) following the manufacturer’s instructions and then diluted to 1 µg/µL ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were removed and the protein concentration was quantified using the colorimetric assay by PierceTM BCA protein assay kit (ThermoFisher Scientific, 23225). Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... The amount of affibody conjugated to the polymer was quantified by dissolving 2-3 mg of HA-Nor-Ox-Affibody in PBS at 2% w/v and determining the protein concentration of the solution using a Pierce 660 Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The membrane pellet was resuspended in the same buffer and the protein concentration was determined spectroscopically with the BCA protein assay kit (Thermo Fisher Scientific). The membrane fraction was supplemented with n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Immunology 2023Quote: ... they are displayed as area ratio and were normalized to protein content measured using Pierce TM BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Twenty-five microliter of the slurry was centrifuged and the supernatant was subjected to protein concentration determination using Pierce TM BCA protein assay Kit (Thermo Fisher Scientific). The protein slurry was diluted to 1 ng/µl with 100 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... HM and IF pooled EV fractions were assayed for total protein according to manufacturer’s instructions (Micro BCA protein assay kit, Thermofisher Scientific, Brisbane, Australia) and a volume equivalent to 20 µg total protein was aliquoted for western blot (WB ...
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bioRxiv - Cell Biology 2022Quote: ... MSC-EVs were not lysed in RIPA buffer and protein content was determined via the Micro BCA™ Protein Assay Kit (ThermoFIsher, 23235).
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bioRxiv - Genetics 2022Quote: ... A protein concentration of the tissue lysates was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and 3-10 µg of the total protein lysate/homogenate used per assay well in duplicates and repeated in three independent runs ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenates were cleared by centrifugation at 13,000 x g at 4°C for 25 min and the protein concentration in the collected supernatant was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein extracts (20 µg of protein for each sample) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...