Labshake search
Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... 2.5 ng total RNA was employed for first-strand cDNA synthesis with High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) followed by a 15 cycles preamplification PCR of genes of interest using the Fluidigm PreAmp Master Mix (Fluidigm Europe B.V. ...
-
bioRxiv - Microbiology 2021Quote: ... Total isolated RNA (1μg) was reverse transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems Cat# 4368814). The resulting cDNA was used for amplification of selected genes by real-time quantitative PCR using Power SYBR Green Master Mix (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNAs were reverse transcribed from 2μg of total RNA by using Applied Biosystems High-Capacity cDNA Reverse Transcription Kits (Thermo Fisher Scientific). Conventional PCR amplification of cDNA was done in a 25μl reaction volume by using DreamTaq Green DNA polymerase (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... of total RNA were reverse transcribed into cDNA by using random hexamers from a high-capacity cDNA reverse transcription kit (Applied Biosystems). TaqMan gene expression Master Mix (Applied Biosystem ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was synthesized using all RNA available (or 1-5 ng) with the High-Capacity Reverse Transcription Kit with RNase Inhibitor (Life Technologies) (250 C for 10 min ...
-
BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... cDNA was derived from 500 ng RNA by reverse transcriptase using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368814) according to the manufacturer’s specifications ...
-
bioRxiv - Immunology 2021Quote: ... cDNA synthesis was conducted using the High Capacity cDNA RT kit according to manufactureŕs guidelines (Applied Biosystems, Foster City, CA, USA). Following ...
-
bioRxiv - Neuroscience 2022Quote: ... 250-1000 ng of RNA was reverse transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). RT-qPCR mix was prepared using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The extracted RNA was stored at −80°C until subjected to reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The obtained cDNA samples were stored at −20 °C until analysed using qPCR.
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from 25 ng of RNA using oligoDT primers in a 20 µl reaction using a High Capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368814). qRT-PCR reactions were performed in real time using 5 µl PowerUp SYBR Green Master Mix (ThermoFisher ...
-
bioRxiv - Immunology 2022Quote: ... RNA was isolated as described above and 500 ng were reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems, 4387406) and analysed by RT-qPCR.
-
bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 1 µg of the extracted RNA was used as a template for cDNA synthesis by high-capacity cDNA reverse transcription kit (Cat. no. 4368814, Applied Biosystems) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... The amount of RNA was measured using the Nanodrop technique and reverse transcribed with random hexamers (High-Capacity cDNA Archive kits; Applied Biosystems), following the manufacturer instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... 1ug of which was converted to cDNA by a RT-PCR reaction using the High Capacity RNA-to-cDNA kit (Applied Biosystems). ISG induction relative to the untreated controls in wild-type cells was measured by qPCR assay (PowerSYBR Green PCR Master Mix ...
-
bioRxiv - Cell Biology 2022Quote: ... and reverse transcribed into complementary DNA (cDNA) using random primers from the High-Capacity cDNA Reverse transcription kit (Thermo Fisher Scientific). Using specifically designed primers ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA was quantified using a ThermoScientific NanoDrop 1000 and 500 to 2000 ng was reverse transcribed with a High-Capacity cDNA Reverse Transcription Kit (Life Technologies). qPCRs were run using the SensiFAST probe No-ROX kit (Bioline ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 1 µg equivalent of RNA was used for first-strand cDNA synthesis using High capacity cDNA reverse transcription kit [Thermo Fisher Scientific (Applied Biosystems), Waltham ...
-
Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... 2 μg of RNA were used to generate cDNA libraries using a High-Capacity cDNA reverse transcription kit (Applied Biosystems, 4368814). RT-qPCR was performed using PowerUP SYBR Green Master Mix (Applied Biosystems ...
-
bioRxiv - Immunology 2022Quote: ... One microgram of RNA of each sample was reverse transcribed with the High-Capacity cDNA Reverse Transcription kit from Applied Biosystems (Applied Biosystems/Life Technologies ...
-
bioRxiv - Genomics 2022Quote: ... and 200 ng of total RNA from each sample was reverse transcribed using the High-capacity RNA-to-cDNA kit (ThermoFisher Scientific). The qPCR reactions were prepared using TaqMan Multiplex Master Mix (ThermoFisher Scientific).
-
bioRxiv - Plant Biology 2022Quote: ... For the cDNA obtention we used 2 μg of the total RNA extract from each sample and the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using 10 pmol random nonamers (Takara ...
-
bioRxiv - Plant Biology 2022Quote: ... concentrated to almost dry on a SpeedVac and used as input for High Select Fe-NTA Phosphoptide Enrichment kit (Thermo Scientific). Phosphopeptides obtained from both enrichment processes were dried using a SpeedVac ...
-
bioRxiv - Immunology 2022Quote: ... of total RNA were reverse transcribed into cDNA by a high-capacity cDNA reverse transcription kit using random hexamers (Applied Biosystems). Cytokine gene expression levels were determined by TaqMan probe (TaqMan ...
-
bioRxiv - Microbiology 2022Quote: RNA from Caco-2 cells was used as the template in reverse transcription reactions using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... 1000 ng of isolated RNA were subjected to cDNA synthesis using the high-capacity cDNA reverse transcription kit (Thermo Scientific, USA), followed by PowerUp SYBR Green Master Mix (Applied Biosystems ...
-
bioRxiv - Microbiology 2022Quote: ... These RNAs were 35 nucleotides long and they were prepared by in vitro transcription using a DNA template (5’-CAGTAATACGACTCACTATAGGGGAAGCGGGCATGCGGCCAGCCATAGCCGATCA-3’) and the TranscriptAid T7 high-yield transcription kit (Thermo Scientific). The reaction was performed in a 50-μl mixture containing 1× TranscriptAid reaction buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 150 ng of total RNA extracted from heart tissues with a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time PCR was performed with QuantStudio (Applied Biosystems ...
-
bioRxiv - Neuroscience 2023Quote: Concentrations of RNA samples were measured by NanoDrop and stored at −80°C until being reverse-transcribed into cDNA with the High Capacity cDNA Reverse Transcription kit (4368814, Thermo Fisher). qRT-PCR was conducted with the TaqMan™ Fast Universal PCR Master mix (4352042 ...
-
bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
-
bioRxiv - Neuroscience 2022Quote: ... n=168 punched samples were analysed).Total RNA was reverse transcribed to generate cDNA using High Capacity reverse transcription kit (Life Technologies) as per the manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... 2 µg of total RNA was used to generate the cDNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific). The reaction was run in QuantStudio 6 Real-Time PCR Systems using 0.2 μl of cDNA in a 15 μl reaction according to the manufacturer’s instruction in triplicate that measures real-time SYBR green fluorescence ...
-
bioRxiv - Immunology 2022Quote: ... Extracted RNA was reverse transcribed to complementary DNA (cDNA) using the High-capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, CA), as per manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... Eluted peptides were lyophilized and reconstituted in 300 µL 0.2% TFA for fractionation using the Pierce High pH Reversed-Phase Peptide Fractionation Kit following the instructions provided by Thermo Fisher Scientific for TMT-labelled samples ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA samples were subjected to retro-transcription using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, 4368813). Quantitative real-time PCR was performed on CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA samples were subjected to retro-transcription using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, 4368813). Quantitative real-time PCR was performed on CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Physiology 2022Quote: ... 1 μg of RNA was reverse transcribed using Oligo dT primers and a high-capacity cDNA Synthesis Kit according to the manufacturer’s instructions (Applied Biosystems, #4368814). Quantitative PCR amplifications were performed using the Power SYBR Green PCR Master Mix (Applied Biosystems ...
-
bioRxiv - Physiology 2022Quote: ... Total RNA (1 µg) was reverse transcribed to cDNA using High Capacity cDNA Reverse Transcription Kits with random primers (Applied Biosystems). qPCR was performed using an ABI Real-Time PCR 7500 system (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40 and MERS-CoV UpE targets were transcribed from 600 ng of linearized and purified plasmids using the TranscriptAid T7 High Yield Transcription kit (Thermo Scientific). The IVT mastermix was set up according to the manufacturer instructions and the reaction was performed at 37°C for 6-7 hr ...
-
bioRxiv - Molecular Biology 2022Quote: ... to remove genomic DNA and then reverse transcribed using a High-Capacity cDNA reverse-transcription kit with random hexamer primers (Applied Biosystems) in accordance with the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1ug of total RNA from each sample was used for reverse transcription using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was reversed transcribed using random hexamer primers with High-Capacity cDNA Reverse Transcription Kit (Catalog No. 4368814, Thermofisher Scientific, USA). For qPCR ...
-
bioRxiv - Biochemistry 2022Quote: ... Four to nine hundred nanograms of total RNA were reverse transcribed using a high-capacity cDNA reverse transcription kit (Applied Biosystems). qPCR was carried out using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... RNA integrity was checked using the Fragment AnalyzerTM (AATI, USA) before cDNA was synthesised from extracted RNA using the High-Capacity cDNA reverse transcription kit (Applied Biosystems). Three technical replicates of gene specific products were amplified in 10 µl reactions using Power SYBR Green Master mix on a CFX384 Touch Real-Time PCR detection system ...
-
bioRxiv - Physiology 2022Quote: ... Complementary DNA (cDNA) was prepared from 1 ug of total RNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) and the resulting cDNA was diluted 1:50 before use ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from reverse transcription on 1.0 μg total RNA in a 20 μL reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, U.S.A.) and T100 thermal cycler (BIO-RAD ...
-
bioRxiv - Cancer Biology 2022Quote: Reverse transcription was performed on 500 ng total RNA using the High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (ThermoFisher, 4374966). Power Up SYBR Green Master Mix (ThermoFisher ...
-
bioRxiv - Microbiology 2024Quote: ... adapters were ligated to the amplicons in a PCR reaction using the P5 and P7-bearing adapter primers and the Phusion High-Fidelity DNA Polymerase kit (Thermo Scientific). Amplicons were sized and purified using an AmpurXP magnetic bead capture (Agencourt) ...
-
bioRxiv - Microbiology 2024Quote: ... The dsDNA fragments were amplified in a PCR reaction with the 454-E primer and the Phusion High-Fidelity DNA Polymerase kit (Fisher Scientific). Amplicons were purified with the NucleoSpin gel and PCR Clean-up (Macherey-Nagel).