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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: BeWO cell culture was harvested 48-hours post transfection media was subjected to lactate dehydrogenase (LDH) detection using the Pierce™ LDH Cytotoxicity Assay Kit (Thermo Fisher, Cat No. 88953), according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: ... The sample was subsequently labeled with the TAMRA-alkyne dye by using the Click-It™ TAMRA Glycoprotein Detection Kit (Thermo Fisher Scientific, Waltham, MA). The mixture was vortexed for 5 seconds after the addition of each component according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 10μM EdU was added to the culture media for 2h prior to fixation and detection using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10424), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The assessment of apoptosis in pMBMECs was performed by immunofluorescence staining with the Image-iT™ LIVE Red Poly Caspases Detection Kit (Thermo Fisher Scientific, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... real-time RT-PCR for AIV diagnosis was done using the VetMAX-Gold AIV Detection Kit (cutoff Ct value = 38 / internal control 25-30 Ct, Applied Biosystems™ Cat No. 4485261) and the Detection Kit Genome amplification (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA concentration was determined by NanoDrop and 800ug of RNA was used as input for cDNA synthesis using the AB High-Capacity cDNA Synthesis Kit (Applied Biosystems). cDNA was diluted 1:10 for used in ddPCR assays ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 1 μg of RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific) as per the manufacturers protocol ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 1 μg of RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific) as per the manufacturers protocol ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 200 ng of total RNA with the High-Capacity cDNA Synthesis kit (Applied Biosystems, Foster City, CA). qRT-PCR was performed using the 2x Fast SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... DNase-treated total RNA was reversed transcribed to a single-stranded cDNA using the high-capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative reverse transcriptase PCR (qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s instructions and 1 μg was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher, USA). qRT-PCR was performed using 25 ng cDNA per reaction and the Power SYBR Green PCR Master Mix (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). The qPCR reaction was performed on a qTOWER3 real-time thermal cycler (Analytik Jena ...
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bioRxiv - Immunology 2021Quote: ... cDNA was then synthesized from the total RNA using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA) on a thermal cycler (Veriti 96-Well Thermal Cycler ...
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bioRxiv - Genetics 2021Quote: 1ug of extracted RNA was used to generate cDNA using a high-capacity cDNA reverse transcriptase kit (Thermo Fisher Scientific, #4374966) using the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... above six were applied to first strand cDNA synthesis using 300ng of RNA and High Capacity cDNA Reverse Transcription kit (Applied Biosystems). The synthesized cDNA from each RNA sample was diluted 1:5 times in nuclease-free water to conduct qPCR.
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bioRxiv - Developmental Biology 2020Quote: ... from 1–2 μg of DNase I treated TNA was synthesised using a High Capacity cDNA Reverse Transcription Kit using random primers (Invitrogen, #4374966). qRT-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was prepared from 1 μg of total RNA purified from the GCs collected at the indicated time points (Basal, PMSG, hCG 4h and hCG 10h) (Fig. 2A) using the High Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific). qPCR was performed using Power SYBR Green Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Physiology 2022Quote: ... and single stranded cDNA was synthesized from total RNA using the High-Capacity cDNA reverse Transcription Kit (Applied Biosystems, Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from 100 ng of total RNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA) and qPCR was carried out with gene-specific assays for MT1A (Hs00831826_s1) ...
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bioRxiv - Bioengineering 2022Quote: ... The RNAs were quantified using a Nanodrop and the retro-transcription made using the high-capacity cDNA reverse transcription kit (Thermo Fisher). qPCRs were done by mixing 3.25 μL of H2O RNAse free from the RNA extraction kit to 6.25 μL of Fast SYBR green Master mix (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of total RNA was used for complementary DNA (cDNA) synthesis using the High Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific). Quantitative expression analysis of targets was performed on a 7500 Fast Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs were then subjected to reverse transcription using SuperScript II (Life Tech) or High Capacity cDNA Reverse Transcription Kits (Applied Biosystems). For Per2AS transcripts ...
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bioRxiv - Molecular Biology 2021Quote: Two µg of total RNA extracted from each cell line was reverse-transcribed using a High-Capacity cDNA ArchiveTM Kit (Applied Biosystems). A two-hundredth aliquot of the cDNA was subjected to qRT-PCR with primers (final concentration 200 nM each ...
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bioRxiv - Molecular Biology 2020Quote: ... was performed as follows: 2 μg of messenger RNA (mRNA) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg purified DNaseI-treated RNA was used for reverse transcription (RT) by High Capacity RNA-to-cDNA kit (Applied Biosystems). 1 μg purified DNaseI-treated RNA was used for reverse transcription (RT ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... cDNA was then used as a template for messenger RNA (mRNA) amplification using the TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific). Amplified (aRNA ...
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bioRxiv - Neuroscience 2019Quote: The first-strand cDNA was synthesized using one-to-two μg of RNA using high capacity cDNA reverse transcription kit (Applied Biosystems) and qPCR was performed on an Applied Biosystems ViiA using Taqman primers (Integrated DNA Technologies ...
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bioRxiv - Physiology 2019Quote: ... cDNA was synthesized from 1µg of RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... Viral RNA samples were reverse-transcribed (RT) to cDNA (High Capacity RNA-to-cDNA Kit, Applied Biosystems, Foster City, CA, USA). To quantify the number of ZIKV genomes we used the cDNA in a quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA (1 µg) from each sample was subjected to first-strand cDNA synthesis with the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) and random hexamers ...
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bioRxiv - Microbiology 2020Quote: ... A 1.5 μg of total RNA was applied to reverse-transcription reaction for cDNA by using High Capacity RNA-to-cDNA Kit (Applied Biosystems). All specific primers for the target genes are listed in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA synthesis was performed using the High Capacity cDNA Reverse Transcription Kit (Ref No. 4368814, Lot No. 00643655, appliedbiosystems by Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... isolated total RNA from slices was converted to double stranded cDNA using the High Capacity cDNA Reverse Transcription Kit (Life Technologies). Input cDNA was used at 2-50 ng per reaction as determined by studies to determine the linear ranges of amplification ...
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bioRxiv - Cell Biology 2019Quote: ... in accordance with the manufacturer’s instructions and then fractionated using a Pierce high-pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Eight fractions were collected ...
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bioRxiv - Plant Biology 2019Quote: ... 2μg of total RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (#4368814; Applied Biosystems, Foster City, USA). cDNA was diluted 20X in ddH2O prior to RT-PCR or qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... 1-2 µg of total RNA for each genotype was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher). qPCR was performed using SYBR Select Master Mix (Thermo Fisher) ...
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bioRxiv - Physiology 2019Quote: ... 1 µg total RNA (RIN scores > 7.0) was then reverse transcribed using a high capacity RNA to cDNA kit (Applied Biosystems, UK) following manufacturers steps ...
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bioRxiv - Genomics 2019Quote: ... Extracted RNA (500 ng to 2 µg) were used as templates to make cDNA libraries using a High Capacity RNA-to-cDNA kit (Applied Biosystems). TaqMan gene expression assays were designed using GAPDH (Hs03929097 ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was prepared by reverse transcribing 99ng total RNA in a volume of 44μl using the High-Capacity RNA-to-cDNA™ Kit (#4387406, Applied Biosystems). qRT-PCR was carried out using 2μl cDNA ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (250 ng) was used to generate cDNA with the High Capacity cDNA Reverse Transcription Kit following the manufacturer’s protocol (Thermo Fisher Scientific). Human MLH3 splice variants were characterized by Reverse Transcription PCR (RT-PCR ...
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bioRxiv - Genetics 2021Quote: ... 1ug of mRNA was used for the cDNA synthesis using the High Capacity cDNA Reverse Transcriptase Kit (4368814) by Applied Biosystems. The qPCR reaction was carried out using KAPA SYBR FAST (KK4602 ...
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bioRxiv - Genomics 2021Quote: ... RNA (1 µg, quantified with NanoDrop) was reverse transcribed to cDNA with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA quantity was assessed using the NanoDrop-1000 spectrophotometer and cDNA was prepared with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Physiology 2021Quote: ... 2000 ng of RNA was used to synthesize cDNA by reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Gene expression was performed by quantitative PCR with iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... treatment and 1 μg DNA-free RNA was reverse transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) on a Mastercycler Thermocycler (Eppendorf ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... 1ug of RNA was converted to cDNA using the High-Capacity RNA-to-cDNA reverse transcriptase kit (ThermoFisher Scientific, Paisley, UK) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... was reverse transcribed to cDNA at 42°C for 60 min using the High Capacity RNA-to-cDNA kit (Applied Biosystems), according to the manufacture’s protocol ...