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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... and 1 µg RNA starting material was reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Germany). Gene expression was analyzed with predesigned QuantiTect primer assays (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, United States) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Pathology 2024Quote: ... 200 ng of total RNA prepared above was used as starting material for cDNA first strand synthesis using High Capacity RNA to cDNA kit (Applied Biosystems). The preamplification was done with TaqMan PreAmp Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Two micrograms of total RNA were reverse transcribed using High-Capacity RNA to cDNA kit (Applied Biosystems, Rhenium, Modi’in, Israel, #4368814). Quantitative PCR analysis was performed using or FAST SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μl of eluted RNA and input RNA was retro transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™ #4368814). RT-qPCR was performed using SYBR GreenER™ qPCR SuperMix for ABI PRISM™ Instrument (ThermoFisher # 11760100) ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 ng of total RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit using random primers (Applied Biosystems). RT-qPCR was performed by the CFX96 Real-Time PCR system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... adapters were ligated to the amplicons in a PCR reaction using the P5 and P7-bearing adapter primers and the Phusion High-Fidelity DNA Polymerase kit (Thermo Scientific). Amplicons were sized and purified using an AmpurXP magnetic bead capture (Agencourt) ...
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bioRxiv - Microbiology 2024Quote: ... The dsDNA fragments were amplified in a PCR reaction with the 454-E primer and the Phusion High-Fidelity DNA Polymerase kit (Fisher Scientific). Amplicons were purified with the NucleoSpin gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... The eluted TMT-labelled peptides were dried by vacuum centrifugation and phosphopeptide enrichment was subsequently carried out using the sequential metal oxide affinity chromatography (SMOAC) strategy with High Select TiO2 and Fe-NTA enrichment kits (Thermo Scientific). Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA was reverse transcribed using the High-Capacity RNA-to-cDNA™ kit (Thermo Fisher Scientific, Cat# 4387406) following the protocol instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNaseI-treated RNA (500 ng) was reverse-transcribed using random primers and a High-Capacity cDNA Reverse Transcription Kit (#4368814, Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription PCR (RT-PCR) was carried out after cDNA synthesis using the High Capacity cDNA reverse transcription kit (Applied Biosystems). Real-time quantitative reverse transcription PCR (qRT- PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of complementary DNA was performed using 1–2 μg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... 500 ng of the total RNA extracted was reverse transcribed into cDNA using the High-Capacity cDNA Transcription Kit (Applied Biosystems) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Resultant RNA was converted to cDNA using 500 ng total RNA and a high-capacity reverse transcription kit (Applied Biosystems, 100025924) with final concentrations of reagents as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted TMT-labelled peptides were dried by vacuum centrifugation and phosphopeptide enrichment was subsequently carried out using the sequential metal oxide affinity chromatography (SMOAC) strategy with High Select TiO2 and Fe-NTA enrichment kits (Thermo Scientific). Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and about 1.8 μg of total RNA was reverse transcribed using a High Capacity RNA to cDNA kit (Applied Biosystems; Thermofisher Scientific). Additional samples in which RNA was not reverse transcribed were included as controls and indicated effective genomic DNA removal.
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bioRxiv - Evolutionary Biology 2024Quote: ... we used 500 ng of RNA to synthesise first strand cDNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). The cDNA from each sample was diluted 1:10 times in nuclease-free water to be used for qPCR ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: Freshly extracted RNA (500 ng per 20µL reaction) was transcribed into cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher). Such cDNA samples were applied as templates for RT-qPCR using 2× GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... Each segment of the LACV genome was amplified utilizing primers specified in Table 5 and a Phusion High-Fidelity PCR Master Mix Kit with HF Buffer (Thermo Scientific). All LACV segments were confirmed by a 1% agarose gel and sent to Sanger sequencing using the primers in Table 5 at Genewiz (South Plainfield ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from RNA (400 ng) with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Real-time amplification was done with RT2 SYBR Green qPCR Mastermix (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) was generated from 400 ng of total RNA using the high-capacity RNA-to-cDNA kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... then reverse transcribed into complementary deoxyribonucleic acid (cDNA) with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Genomics 2023Quote: ... 2ug of RNA was transcribed into cDNA using the High-Capacity cDNA Reverse Transcription kit (AB Applied Biosystems, catalogue number: 4368814) following the published protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Ten μl of total RNA extract was used for cDNA synthesis using High Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Standard reverse transcriptase was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies, Foster City, CA). Transcript mRNA levels were determined by rt-qPCR on the ABI Prism 7900HT Real Time System (Applied Biosystems ...
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bioRxiv - Physiology 2023Quote: ... Standard reverse transcriptase was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies, Foster City, CA). Transcript mRNA levels were determined by rt-qPCR on the ABI Prism 7900HT Real Time System (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... One thousand nanograms of RNA was used to synthesize complementary DNA (cDNA) using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qRT-PCR reactions were performed on a QuantStudio 7 Flex PCR machine using PowerUp Sybr Green Master Mix (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... jejuni NCTC11168 WT and RNase deletion strains were reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). One microgram of RNA was denatured ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA transcription into complementary DNA was performed using the High Capacity cDNA Reverse Transcription Kit in Veriti 96 Thermocycler both from Applied Biosystems. Finally ...
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bioRxiv - Pathology 2023Quote: ... cDNA was made with 2000 ng of RNA per reaction utilizing the High-Capacity cDNA Reverse Transcription Kit according to the manufacturer’s protocol (Thermofisher cat# 4368814). qPCR was performed using 50 ng per reaction with PowerUp SYBR green master mix and final concentration of 2.5 μM of forward and reverse primers (Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from either 1 μg (tissue) or 100 ng (organoid) of total RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using SYBR™ Green master mix (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 μg total RNA from each stimulated sample using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (4374966, Applied Biosystems, Foster City, CA, USA). Quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Molecular Biology 2022Quote: cDNA for real-time quantitative PCR (qPCR) was generated from total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time qPCR reactions were performed in triplicate as described (Risco et al. ...
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bioRxiv - Immunology 2023Quote: Dried peptides were re-suspended in 50 µL Fe-NTA binding buffer (0.1% TFA, 80% acetonitrile; included in High-SelectTM Fe-NTA Phosphopeptide Enrichment Kit, Thermo Scientific #A32992).
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bioRxiv - Cancer Biology 2022Quote: ... The purity and concentration of the extracted RNA were determined at 260/280 nm using a spectrophotometer (Nanophotometer) and were reverse transcribed into cDNA with a High Capacity cDNA conversion Kit (Applied Biosystems) using 2 μg of total RNA ...
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bioRxiv - Neuroscience 2023Quote: 100 ng of RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Sci., Cat. no: 4374966) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Equal amounts of RNA (0.5–1μg) were reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, #4368813) with the random primers provided ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (qPCR) was performed using cDNA generated with the High Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific, # 4368813) with SYBR Select Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... was performed on 200 ng to 1 µg DNase I-treated RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCRs (qPCRs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The barcodes were amplified from 100 ng DNA and a final primer concentration of 0.1 µM using the Phusion® High-Fidelity PCR Kit (Thermo Scientific) with individually optimised annealing temperatures between 62 and 65°C ...
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bioRxiv - Neuroscience 2023Quote: ... one µg of total RNA was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA). Quantitative analysis of target gene expression was performed on a 7500 Applied Biosystems real-time PCR system (Foster City ...
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bioRxiv - Microbiology 2023Quote: ... Multiplexed TMT samples were separated into four fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). TMT labelled fractions were analysed in an Orbitrap Fusion Lumos using a resolution of 50,000 ...
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bioRxiv - Plant Biology 2023Quote: ... Two μg of total RNA were subjected to reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, MA) with oligo(dT ...
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bioRxiv - Physiology 2023Quote: ... 1 μg of RNA was converted to cDNA using the High-Capacity RNA to cDNA kit (Applied Biosystems, Foster City, CA). For qRT-PCR ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg of total RNA was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA). Quantitative analysis of target gene expression was performed on a 7500 Applied Biosystems real-time PCR system (Foster City ...