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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP) (Gehl et al., 2009) by Gateway® cloning (Invitrogen, California) from the pENTR clones to yield the constructs as listed in Supplementary table S2 ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescent transgenic or mRNA injected embryos (injection protocols in [33]) were mounted in 2% low melting agarose gel (Thermo Fisher Scientific; 16520100) at 512-cell stage and imaged using SP8 LSCM or spinning disk confocal microscopes.
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bioRxiv - Genomics 2019Quote: ... The DNA purity was determined by 0.8% agarose gel 100 V electrophoresis for 40 min and DNA concentration was determined by Qubit® 2.0 fluorescent meter (Invitrogen, Carlsbad, USA). Following quality assessment ...
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bioRxiv - Cancer Biology 2020Quote: ... SALE-Cas9 and SALE-Cas9 RIT1M90I cells were transduced with YAP5SA lentivirus and 48 hours post-transduction GFP-expression was confirmed by direct fluorescent expression using the EVOS FL Color imaging system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... HCLE cells were cultured to confluence on glass bottom dishes and pre-loaded with 5 μM Fluo-3AM fluorescent dye (Invitrogen, Carlsbad, CA) to allow for Ca2+ visualization ...
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bioRxiv - Biophysics 2019Quote: ... for biplane analysis were obtained by running a Z stack of 200 steps (with an axial step size 50 nm) of sparse sub-resolution (200 nm) dark-red fluorescent beads (ThermoFisher FluoSpheres F8807). A number of such beads are selected ...
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bioRxiv - Microbiology 2019Quote: ... of representative isolates from each dendrogram cluster was used as template in cycle sequencing reactions using both pA and pH primers with fluorescent dye-labelled terminators (Applied Biosystems, USA). The sequencing was performed in a 3130xl Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... patch pipettes were filled with the same cesium-base solution described above supplemented with 50 µM of the red fluorescent indicator Rhod2 (R14220, Invitrogen™, Thermofisher Scientific). The Ca2+ dye was excited with a 543 nm Helium Neon Laser system (Melles Griot 05-LGR-193-381 ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent actin was prepared by labelling monomers with Alexa Fluor 649 carboxylic acid succinimidyl esther (Molecular Probes, Life Technologies, Carlsbad, CA, USA). Before use ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent actin was prepared by labelling monomers with Alexa Fluor 649 carboxylic acid succinimidyl esther (Molecular Probes, Life Technologies, Carlsbad, CA, USA). Before use ...
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bioRxiv - Microbiology 2020Quote: ... 1.3 µL of 1 mg/mL stock of the lipophilic fluorescent dye BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, 493/503, Molecular Probes, ThermoFisher Scientific, USA) per sample was added to stain for neutral lipids (TAG) ...
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bioRxiv - Cell Biology 2021Quote: ... The channels were then carefully aligned using alignment parameter from control measurements with 0.5 μm diameter multi-spectral fluorescent beads (Invitrogen, Thermo Fisher Scientific). For visualization of the Golgi surface ...
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bioRxiv - Biophysics 2020Quote: ... The biotinylated surface was then incubated with FluoSpheres™ NeutrAvidin™-marked microspheres (200nm in diameter, fluorescent in yellow-green, ThermoFisher, F8774) with 1-5uL in 150uL PBS per hydrogel ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane potential was determined by incubating 300’000 cells for 40 minutes with 100 nM of the fluorescent probe DiBAC4(3) (Thermofisher, ref. no. B438) in 6-well plates in 1 mL media ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed x3 in PBS or TBS and incubated with fluorescent secondary antibodies and dyes (DAPI, Cell Signaling Technology; Phalloidin, ThermoFisher) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated for one hour at room temperature with secondary antibodies conjugated with Alexa Fluor fluorescent probes (Thermo Fisher Scientific) diluted in 10 % horse serum ...
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bioRxiv - Microbiology 2020Quote: ... followed by qPCR using HIV-1 primers and probes (specific for transcription of the first nucleosome nuc1a or gag/u1a) and the housekeeping genes 18S and GAPDH (both containing VIC&trade/TAMRA&trade fluorescent probe; Applied Biosystems, USA) as controls (see list of primers).
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 0.5 μM tetramethylrhodamine (TMRM; a cell permeant fluorescent red-orange dye as mitochondrial membrane potential indicator) (Thermo Fisher Scientific,T668) and Hoechst dye (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 500 μL of exponentially growing cells in TY were centrifuged and resuspended in 100 μL of PBS supplemented with the fluorescent membrane dye FM 4-64 (Molecular Probes, Invitrogen) at 1 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: Each section of the mOFC was collected directly onto a slide and was stained with NeuroTrace fluorescent nissl (Invitrogen, Eugene, OR, USA) and left to dry in darkness for at least 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell invasion distance was measured from the bead surface using ImageJ from images acquired via fluorescent microscopy after fixing and staining cells with DAPI (Invitrogen, Carlsbad, CA) (10 µg/mL in 1X PBS) ...
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bioRxiv - Plant Biology 2019Quote: Membrane permeabilization of fungal cells was analyzed using confocal microscopy by visualizing the influx of the fluorescent dye SYTOX Green (SG) (Thermo Fisher Scientific). Fresh conidia or germlings mixed with 2 x MIC (3 µM for B ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genotyping was performed with four-color fluorescent capillary electrophoresis using an Applied Biosystem Prism 3100 sequencer (Applied Biosystems, Foster City, CA, USA), and alleles were scored using GENEMAPPER v4.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Processed SIM images were aligned via an affine transformation matrix of predefined values obtained using 100 nm multicolor Tetraspeck fluorescent microspheres (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Gene-edited U2OS cells expressing Monocarboxylate Transporter 1 fluorescently labelled with Enhanced Green Fluorescent Protein (MCT1-EGFP) in both alleles were grown in DMEM/F12/GlutaMAX (Cat. No. 10565042, Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (Cat ...
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bioRxiv - Genetics 2019Quote: ... Fragment length was detected by fluorescent capillary fragment analysis on an ABI 3730s Genetic Analyzer and all data analyzed using the GeneMapper software (all Thermo Fisher Scientific). Data was quality controlled for null allele overrepresentation and deviation from Hardy-Weinberg Equilibrium using Micro-Checker(45 ...
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bioRxiv - Zoology 2020Quote: We examined the PVL for sperm and germinal disc samples for embryonic cells by staining them with the fluorescent DNA marker Hoechst 33342 (Thermo Fisher Scientific), and inspecting them under a fluorescence microscope with a BP 340-380 excitation filter and LP 425 suppression filter (Birkhead et al ...
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bioRxiv - Genomics 2020Quote: ... diffraction- limited images of spatially separated broad-range emitting fluorescent beads were acquired across all three colors (Tetraspecks, 100 nm, Life Technologies, T7279). The precise localizations of the beads were obtained by independently fitting their diffraction-limited Point Spread Functions (PSF ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... surface lysines on MS2 capsids were modified with fluorescent dyes (NHS-Atto488 or NHS-Atto633) and the heterobifunctional maleimide-PEG24-N-hydroxysuccinimide ester crosslinker (Thermo Fisher Scientific). Second ...
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bioRxiv - Systems Biology 2019Quote: ... with 5’-amino modification and were coupled with NHS (N-hydroxysuccinimide)-ester labelled fluorescent dyes (Alexa 488, 594, 647 (Thermo Fisher Scientific) and Cy3B (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: The sample consisting of 3D fluorescence nanospheres was prepared by diluting yellow-green 100 nm fluorescent beads (FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm F8803, ThermoFisher Scientific, USA) into water at a concentration of 3 × 109 particles/mL ...
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bioRxiv - Biophysics 2019Quote: ... in vitro (DIVs) 3D neuronal cultures were loaded with the fluorescent calcium indicator Fluo-4 AM (5 µM) (F14201, Thermo Fisher Scientific), in extracellular recording solution (95 mM NaCl ...
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bioRxiv - Genetics 2019Quote: ... Signal amplification after the RNAscope Multiplex fluorescent v2 assay was performed with TSA plus fluorophores as recommended and Alexa Fluor conjugated secondary antibodies (Thermo Fisher Scientific). Complete details of RNAscope probes used here are provided in Table S6.
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bioRxiv - Neuroscience 2020Quote: ... and a 384-channel Neuropixels 3A probe (https://www.neuropixels.org/) coated in a fluorescent dye (CM-DiI, DiI, DiO, Thermofisher Scientific; JF-669, Tocris) was slowly lowered into the brain ...
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bioRxiv - Neuroscience 2021Quote: ... The point spread function of the microscope was estimated from the FWHM value of the x-,y- and z- intensity line profiles from 3D images of 200nm yellow-green fluorescent latex beads (FluoSpheres, F8811, Thermo Fisher Scientific): PSF810x/y = 325 ± 27 (SD ...
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bioRxiv - Developmental Biology 2021Quote: ... or 1 pmol of rif1-MO or 2 pmol of standard control MO together with a fluorescent tracer (dextran fluorescein lysine, Thermo Fisher Scientific) were microinjected into fertilized eggs ...
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bioRxiv - Cell Biology 2020Quote: Virus concentration on the CytoFLEX S and LSR Fortessa was normalized using 200 nm fluorescent polystyrene (Green FluoSpheres, Cat# F8848, ThermoFisher Scientific, USA) spike-in beads ...
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bioRxiv - Biophysics 2021Quote: ... The nucleic acid-specific fluorochrome SYTO 13 green fluorescent nucleic acid stain in 5 mM solution in DMSO (Molecular Probes, Eugene, OR) was used in all experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with fluorescent secondary antibodies (Goat anti-mouse IgG DyLight 800 ThermoFisher #SA5-10176 and Goat anti-rat IgG DyLight 800 ThermoFisher #SA5-10024), and images acquired on a LI-COR Odyssey instrument ...
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bioRxiv - Molecular Biology 2022Quote: ... ΔfcyA::sgfp and Δuprt::k2s fluorescent mutants were co-inoculated in liquid AMM in Nunc96 microplates (Thermo Scientific Inc., Waltham, MA, USA). For each strain 1×104 spores in a total volume of 25 μL 2× AMM were inoculated in each well ...
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bioRxiv - Cancer Biology 2022Quote: ... were designed using GeneArt (https://www.thermofisher.com/ie/en/home/life-science/genome-editing/geneart-crispr/geneart-crispr-search-and-design-tool.html) and cloned into the GeneArt™ CRISPR Nuclease Vector with OFP (orange fluorescent protein) Reporter (ThermoFisher, Cat# A21174). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with unsupplemented neurobasal media and loaded with 200μM 2’,7’-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye (Thermo Fisher Scientific, MA) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: We estimated the holographic uncaging resolution of our customized SLM microscope experimentally by imaging sub-resolution fluorescent beads (100 nm FluoSpheres, Molecular Probes, USA) embedded in 2 % agarose (Cole et al. ...
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bioRxiv - Neuroscience 2023Quote: ... every fourth section was collected onto glass slides and coverslipped with Prolong antifade mounting medium with a fluorescent stain for DAPI (Invitrogen, catalog #P36962). Cannula tracks were identified under a light microscope (Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... we used 0.2 μm diameter carboxylate-modified polystyrene fluorescent microspheres (FluoSpheres, far red/FR λ=660/680, 2% solid diluted in water, F8807, ThermoFisher Scientific) that were activated to promote covalent coupling with primary amines of proteins exposed on the parasite surface ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein content of each spotted lysate was assessed by fluorescent staining with Sypro Ruby Protein Blot Stain according to the manufacturer’s instructions (Molecular Probes, Eugene, OR).
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bioRxiv - Molecular Biology 2023Quote: ... we followed the same procedure as described earlier in the presence of LysoTracker ™ Blue DND-22 fluorescent acidotropic probes for labeling and tracking acidic organelles in live cells (L7525, ThermoFisher, USA). The staining was carried out according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Incorporated AHA was detected using Fluorescent Non-canonical Amino Acid Tagging (FUNCAT) via cycloaddition of alkyne-conjugated Alexa Fluor 647 (Thermo Scientific A10278) (Dieterich et al. ...