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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... RNA samples were diluted 1:100 prior to RT-qPCR with Power SYBR Green RNA-to-Ct 1-step kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... F2 progeny of all crosses were screened for fzo-1(tm1133) and/or pdr-1(gk448) mutations using a single worm PCR Phire Animal Tissue Direct PCR Kit (Thermo Scientific). The resultant amplification products were visualized by gel electrophoresis ...
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bioRxiv - Bioengineering 2021Quote: ... solution (1 mg/mL in DMEM) with 1:50 Cy5 dye-labeled fibronectin (conjugated in house using dialysis kit from Life technologies) was added on the chip and incubated for 1 h at room temperature ...
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bioRxiv - Pathology 2022Quote: ... 40 μL of elution buffer (from the kit) and 20 μL of 1:1 NuPAGE sample reducing agent (Thermo Scientific, NP0009) and NuPAGE LDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and 1 million cells were incubated with 1:100 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Life Technologies), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 8% CO2 with 125 rpm rotation to a density of 3.0 x 106 live cells per mL of culture were transiently transfected with an appropriate plasmid at 1 μg of DNA per 1 mL of culture using the Expifectamine transfection kit (Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then covered with a mix of 1 ml of Reagent A and 1 ml of Reagent B from a Pierce ECL western blotting substrate kit (Thermofisher, 32106). Excess liquid was removed ...
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bioRxiv - Microbiology 2020Quote: ... were grown overnight in 600 μL of LB at 37°C in a 1 mL-volume 96-deep-well plate (Thermo Scientific; catalogue number 260251), aliquoted in 96-well plates with 15% glycerol ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Bioengineering 2021Quote: ... The sections were cultured statically in a 24 well plate and incubated for and additional hour before adding cell culture media (DMEM, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA) for 5 days ...
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bioRxiv - Cell Biology 2021Quote: Plasmid transfection into NIH3T3 cells was carried out with 500 ng DNA per well of a 24-well plate using 1 µl Lipofectamine LTX (Thermo Fisher Scientific, Cat# 15338100) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: INS-1E or INS-1 832/13 cells were seeded in a 12-well plate and transfected with siRNA using RNAiMAX (Thermo Fisher Scientific, Cat# 13778150). ON-TARGET plus SMARTpool siRNA was used for knocking down rat Wfs1 (Dharmacon ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid transfection into ARPE-19 cells was carried out with 500 ng DNA per well of a 24-well plate using 1 µl Lipofectamine LTX (Thermo Fisher Scientific, Cat# 15338100)) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR success was evaluated on a 1 % agarose gel before PCR products were normalised using a SequalPrep Normalisation plate (Thermo Fisher Scientific, MA, USA) following the manufacturer’s instructions with an end concentration of 25 ng per sample (100 μl) ...
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bioRxiv - Immunology 2022Quote: ... A pre-titrated dose of pseudovirus was incubated with eight serial 5-fold dilutions of serum samples (1:20 start dilution) in duplicate in 384-well flat-bottom tissue culture plates (Thermo Fisher, #12-565-344) for 1 hour at 37°C prior to adding 293T/ACE2 cells ...
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bioRxiv - Bioengineering 2022Quote: ... The isolated cells were washed and plated on non-tissue culture treated plates in complete RPMI (10% fetal bovine serum [FBS, Gibco], 1% penicillin/streptomycin [Gibco]) supplemented with 20ng/mL of M-CSF [Miltenyi Biotech]) ...
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with DENV-1 Mochizuki strain (in-house product) was coated onto a Nunc Maxisorp ELISA plate (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Immunology 2021Quote: ... the virus-serum mixture was added to 1 x104 Vero or Vero E6 cells seeded 3 h before in 96 well plates (Thermo Fisher Scientific, Ulm, Germany). Cells were incubated for 4 days at 37°C in a humidified atmosphere containing 6% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were plated on 6-well plates at 1 × 105 cells/well or onto 4-channel chamber slides (Lab-Tek II chamber slide, Thermo Fisher Scientific, Waltham, MA) at 5 × 104 cells/well and were grown until reaching 70% confluence in serum-free EpiLife medium supplemented with HKGS.
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... N2a cells grown on coverslips were transferred to 35 mm culture plates containing 1 ml of serum and antibiotics free Opti-MEM (Gibco, ThermoFisher Scientific, Waltham, MA, USA). Then ...
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bioRxiv - Microbiology 2021Quote: ... Specifically, 1 μg/ml purified hACE2 protein (Kactus, ACE-HM501) was coated onto a 96-well ELISA plate (Thermo Fisher Scientific, 44-2404-21) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ONH astrocytes were seeded at 1 x 104 cells/cm2 on sterilized glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ONH astrocytes were seeded at 1 x 104 cells/cm2 on sterilized glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 24 h ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... The plate was washed for three times and incubated with 100 uL of streptavidin poly-HRP (1:2000 dilution; Thermo Fisher Scientific, Waltham, MA) at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: Both WT and Cx43 KO OCY454 cells following thawing were seeded on rat type I collagencoated plates and grown at 33° C in standard OCY454 media (Supplemental Table 1: αMEM with 10% FBS (Gibco: 26140-079; Lot # 2496659RP) and 2% P/S) ...
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bioRxiv - Genomics 2023Quote: We adapted the neurogenin 2-based neuronal differentiation protocol from (Fernandopulle et al. 2018) using plates and coverslips coated for 1 hour at 37°C with Geltrex (Thermo Fisher Scientific, cat# A1413302) diluted to 1% with DMEM/F12 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was adjusted to 5 µg ml-1 in a total of 100 µl per well diluted antigen and were coated into Pierce™ Nickel Coated Plates (Thermo Fisher Scientific, #15142) at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in PBS at an optical density at 600 nm (OD600nm) of 1 before inoculation into 96-well culture plates (Thermo Fisher Scientific, Waltham, MA) at a final OD600nm of 0.01 in mMRS ...
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bioRxiv - Cancer Biology 2023Quote: ... and were seeded at a density of 1 x 104 cells/well (200 µl culture medium per well) into Nunclon™ Sphera™ 96-well -low attachment plates (Thermo Fisher Scientific) respectively ...
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bioRxiv - Bioengineering 2023Quote: After diluting cells from culture or colonies (see above) 1:10 into PBS+0.1% Tween (total volume=200 μL) in 96-well round-bottom plates (Fisher Scientific cat #07-200-760), samples were measured for cellular composition using a ThermoFisher Attune NxT V6 Flow Cytometer at UW-Madison’s Carbone Cancer Center ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at a density of 1×104 cells/well (200 µl of culture medium per well) into Nunclon™ Sphera™ 96-well low attachment plates (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... optical densities were determined in 200 µl culture in microtiter plates by spectrophotometry in a plate reader (Spectramax M5, Applied Biosystems, United States) and 20 µl of culture were transferred into 4 ml of fresh MMAB-medium ...
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bioRxiv - Cancer Biology 2020Quote: ... The specimen itself was floated on the water surface inside a standard multi well plate (Thermo Scientific Nunclon Delta Surface 96 well plates). Those are needed to be tape sealed in order to prevent water creep under ultrasound irradiation ...
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bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
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bioRxiv - Neuroscience 2020Quote: ... or NAD standard were combined with 50 μl of reagent in a 96-well plate (Nunc™ F96 MicroWell™ White Polystyrene plate, ThermoFisher Scientific). Luminescence was recorded at approx ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were loaded on cellulose TLC plates (20L×L20Lcm, EMD Millipore™ Precoated TLC and PLC Glass Plates, Cat#: M1057160001, ThermoFisher Scientific) and developed in two solvent systems ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... The lysate was transferred to a clear flat-bottom 96-well plate to measure absorbance at 595 nm using a Varioskan plate reader (Thermo Scientific, Cat#: VL0000D0) and SkanIt Software for Microplate Readers ...
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bioRxiv - Microbiology 2023Quote: ... coli phenotypic morphology (pink, round) was picked from each plate and streaked for isolation onto blood agar plates (Thermo Fisher Scientific, Lenexa, KS) which were incubated at 35°C for 18-24h ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μl forward primers (5 μM) and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Biophysics 2021Quote: ... a stack of z-scan images over an axial range of 7 μm in steps of 100 nm of immobilized tetra-spectral fluorescent beads was recorded (TetraSpeck™ carboxylate micro-spheres, 0.1 μm, T7279, Thermo Fisher Scientific). Fig ...
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bioRxiv - Cell Biology 2020Quote: Intracellular pH (pHi) measurements were performed by monitoring intracellular free H+ concentration using the pH-sensitive fluorescent dye BCECF-AM (B1170; Thermo Fisher Scientific) as described previously.19,20 Details of the experimental setup and solutions used are provided in the Supplementary Materials.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... in PBS at 200ng/ul was microinjected directly into the cytoplasm together with fluorescent reporter Dextran Texas Red (MW 70000 Da) (D1864, Invitrogen, Molecular Probes) that facilitates identification of successfully injected neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed 3 times with PBS and were mounted on the slides using Fluorescent-G mounting medium (Invitrogen, Carlsbad, CA). Final concentrations of primary Abs were used as follows ...