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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... A square glass window (2 mm x 2 mm) of No 1 cover glass (0.13 to 0.17 mm thick, Thermo Fisher Scientific, USA) was placed over the craniotomy and the edges were sealed with cyanoacrylate glue (3M ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2021Quote: ... Japan), 50 μg/mL streptomycin (Meiji Seika Pharma, Japan) and 0.1 mM 2-mercaptoethanol (2-ME; 21985-023, Thermo Fisher Scientific). For the maintenance of PKA-ESCs ...
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bioRxiv - Genetics 2021Quote: ... and 1.5 µg of each of the sgRNAs plasmids (PX330-Nanog-sgRNA1/2 and PX330-Esrrb-sgRNA1/2) using 16.5 µl of lipofectamine 3000 and 22 µl of P3000 (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... cell pellet of 1 x106 – 2 x106 PBMCs was resuspended in 2 ml erythroblast cell expansion media containing basal blood media Stempo34 SFM (Thermo Fisher) supplemented with 1x Stempo34 nutrient supplement (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... One-tenth volume of cDNA (2 µl) was added to qPCR reactions containing target specific primers (Table 2) and SYBR green master mix (Applied Biosystems) as per manufacturer’s specification ...
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bioRxiv - Microbiology 2022Quote: ... U-2 OS cells were transduced with lentiviral vector encoding shBmal1 and cells selected using 2 μg/ml of puromycin (Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... The resulting peptic peptides were collected on a trap column cartridge (2 mm x 2 cm) that was filled with POROS 20 R2 reversed-phase resin (Thermo Scientific) and kept at 0.5 °C for 3 min ...
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bioRxiv - Genomics 2023Quote: ... were added into a fresh 2 mL microcentrifuge tube and washed with 1 mL of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific) for a total of three washes (1 min incubation/each) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 variants were identified by the targeted sequencing approach with the Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... RNA pellets were resuspended with 16 µL of nuclease-free H2O and treated by DNase (2 µL of DNase buffer and 2 µL of TURBO DNase; Thermo Fisher) at 37 °C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... The BALF cell pellet was treated with red cell lysis buffer and re-suspended in Hank’s Balanced Salt Solution (HBSS) supplemented with 2% fetal bovine serum (FBS) (2% FBS/HBSS; Gibco, CA, USA) for cell counting and flow cytometry analysis.
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bioRxiv - Immunology 2023Quote: ... cells were stained at 37°C for 30 min with 2 µM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA; Thermo Fisher Scientific) or 1 µM MitoSOX (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of 1.1 mg/mL or 0.5 mg/mL of complex were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids84 prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Neuroscience 2024Quote: ... NPCs were cultured in 2% B-27-supplemented Neurobasal CTS Media (plus 2 uM L-glutamine and 0.2% vol/vol penicillin–streptomycin; Thermo Fisher Scientific) plus 20 ng/ml BDNF ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Immunology 2024Quote: ... Efferocytosis was quantified by incubating BMDMs with Jurkat T cells (MOI 2, 45 min) which had been labelled with calcein-acetoxymethyl (2 h, 37 °C, ThermoFisher Scientific) and exposed to UV light (305 nm ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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bioRxiv - Immunology 2024Quote: ... Digestion was stopped by adding acetonitrile to 2% and trifluoroacetic acid to 0.3% and the beads were collected with DynaMag-2 Magnet (Thermo Fisher Scientific). Digested peptides were purified by loading the supernatant into C18 StageTips (58) ...
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bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
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bioRxiv - Physiology 2023Quote: ... Sense and antisense sequences targeting daf-2 or aak-2 were amplified from N2 lysates and subcloned into donor vectors using Gateway Cloning Technology (Life Technologies). The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Microbiology 2023Quote: 3 μL of 3 mg/mL purified Umb1 particle samples were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Microbiology 2023Quote: ... after which metal beads with DNA absorbed on their silica surface were recovered using the DynaMag™-2 Magnet tube holder (12321D-DynaMag-2 – Invitrogen) and washed twice with AMMLAV/E buffer (10 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2023Quote: ... was pre-complexed at 5µg/mL with PE-conjugated goat anti-Human IgG diluted at 1/100e (Supplementary Table 2) for 2 h at room temperature in PBS CaCl2 MgCl2 (14040133, ThermoFisher Scientific). 200 000 T-ALL cells pre-stained with cell surface markers were incubated with this complexe ...
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Microbiology 2022Quote: ... Filters were first cut into 2 mm by 2 mm pieces using scissors sterilized and treated with RNase AWAY surface decontaminant (Thermo Scientific). Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... were added to Au-flat 2/2 (2 µm hole, 2 µm space) cryo-electron microscopy grid (Protochips, Morrisville, NC, USA) using a Vitrobot Mk IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
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bioRxiv - Neuroscience 2023Quote: ... The following day cells were rinsed with PBS and incubated for 2 hours with the appropriate Alexa Fluor-conjugated secondary antibodies (2 μg/mL, Thermo Fisher) and DAPI (1 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
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bioRxiv - Genomics 2023Quote: ... total RNA from young livers were spiked with 2 µl of a 1:100 dilution of ERCC Ex-fold Mix 1 while that from old livers were spiked with 2 µl of 1:100 dilution of ERCC Ex-fold Mix 2 (Thermo Fisher). Exfold ERCC spike-ins are provided in two mixes ...
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bioRxiv - Biochemistry 2023Quote: ... The peptic peptides thus generated were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...
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bioRxiv - Immunology 2024Quote: ... 2.7 μl of the mixture was pipetted to Quantifoil R 2/2 gold grids with vitrobot Mark IV (Thermo Fisher Scientific) using parameters set to 4 °C with 95% humidity and vitrified with no wait time ...
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bioRxiv - Cancer Biology 2024Quote: ... an IonPac AS24 analytical column and IonPac AG24 guard column (2 x 250 mm and 2 x 50 mm, Thermo Scientific) were connected to a Dionex Ultimate 3000 HPLC system (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids72 prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5.5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Genomics 2024Quote: ... Slides were blocked with 2% BSA in PBS and 4,6-diamidino-2-phenylindole (DAPI; D1306, Thermo Fisher Scientific, Waltham, MA, USA) for 5 minutes and permeabilized with 0.1% Saponion (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 11.2 and 20.2 µg/mL) concentrations as well as the following additional small molecule inhibitors were collected: puromycin (2 µg/mL; ThermoFisher, A1113803), rapamycin (9.1 nM ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 μg/mL Penicilin-Streptomicin) supplemented with 2 mM L-Glutamine (ref.07100, Stemcell) and 10 mM 2-Mercaptoethanol (ref.11528926, Thermo Fisher). The medium was partially changed every other day extremely slowly to prevent disturbing the EB formation ...
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bioRxiv - Biochemistry 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids93 prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5.5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Cell Biology 2024Quote: ... The dishes were then filled with 2 mL of serum-free DMEM-F12 medium supplemented with 2 mM thymidine and 0.02 mg rutin (Acros Organics, #132390050) to avoid GFP photobleaching.