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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... BrdU (5-bromo-2’-deoxyuridine) (11594167, Invitrogen™) was administered 6h later i.p ...
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bioRxiv - Cell Biology 2024Quote: ... with Glutamax (2 mM; GIBCO, ThermoFisher, Waltham, MA), N-acetyl cysteine (NAC ...
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bioRxiv - Cell Biology 2024Quote: ... with Glutamax (2 mM; GIBCO, ThermoFisher, Waltham, MA), N-acetyl cysteine (NAC ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2 mM L-glutamine (ThermoFisher Scientific), 1x N-2 supplement (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 units per embryo of SuperAseIn (Invitrogen, AM2694) were added to stop the reaction.
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bioRxiv - Developmental Biology 2024Quote: ... 2% fetal bovine serum (Gibco, 16-000-044), 200 μM adenosine (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 ml DMEM/F-12 medium (Gibco, 11330107) supplemented with 10% FBS (HyClone ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1 mM 2-mercaptoethanol (Gibco, Cat# 31350-010), 0.1 mM L-glutamine (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM GlutaMAXTM supplement (Life Technologies, 35050-061), 100 µg/mL Primocin (InvivoGen ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Cell Biology 2020Quote: ... Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C and 100% humidity.
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were digested with both trypsin and chymotrypsin and the resultant peptides were loaded onto a reverse phase C18 trap column (Acclaim PepMap 100, 75 μm x 2 cm, nano viper, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using an Ultimate 3000 and washed with 50 μL of solvent A at 10 μl/min ...
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bioRxiv - Neuroscience 2020Quote: ... CO slices were perfused at a rate of 2-3 mL/min in oxygenated MitoTracker staining solution containing: ACSF and MitoTracker™ Red CMXRos (Invitrogen™, M7512, 100nM) for 30 minutes at 29–32°C in the dark ...
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bioRxiv - Developmental Biology 2020Quote: ... (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® F C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) was purchased from Molecular Probes (Melbourne, VIC, Australia). The TiO2 was collected from a disassembled column ...
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bioRxiv - Cell Biology 2021Quote: ... We isolated neonatal cardiomyocyte (postnataTl 2 – 3 days) from Gja1M213L/M213L and Wildtype (WT) mice using PierceTM Primary Cardiomyocyte Isolation Kit (Thermo Fisher Scientific, Walthman, MA) following manufacturer protocol ...
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bioRxiv - Physiology 2023Quote: ... HBMECs were washed by 2% BSA (3×, 5min) followed by secondary antibody incubation with either Alexa Fluor 488 goat anti-mouse (ThermoFisher Scientific; Catalogue number: A11001) or Alexa Fluor 555 goat anti-rabbit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Brains were quickly transferred to 10 μL of the same saline in a 200 μL microcentrifuge tube kept at 2-3°C in a Boekel Scientific TropiCooler Benchtop Incubator (model 260014, Fisher Scientific, Ottawa, ON, Canada), and once 30 brains were collected (2-2.5 min per brain ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Immunology 2024Quote: Vero-CCL81 [for MuV and VSV_RUBV] or Vero-hSLAM [for MeV-IC323] target cells which were seeded at a density of 2 × 104 cells per well in flat-bottom 96-well plates (Fisher Scientific, #08-772-3). The cells were incubated at 37 °C/5% CO2 overnight (∼20 hours) ...
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bioRxiv - Neuroscience 2023Quote: ... and IVH-PHH (Supplementary Figure 2) P7 rats underwent intraventricular injection of 20 µl of 3 kDa Red Dextran Tetramethyl rhodamine (RD/TMR) (D3307, Thermo Fisher Scientific, Waltham, MA), 1.9-nm (1102 ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were washed 3 times in DPBS and incubated for 2 hours with Alexa Fluor 647-conjugated anti-mouse secondary antibody (Thermo Fisher Scientific, 1:1000) in blocking buffer at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and ∼5-10 µL of peptide was loaded onto a reverse-phase trap column (Acclaim PepMap 100, 75 µm × 2 cm, NanoViper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, USA) using an Ultimate 3000 autosampler and washed with 40 µL of buffer A at a flow rate of 20 µL min−1 ...
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bioRxiv - Systems Biology 2024Quote: ... 2-3 butanediol, and erythritol), and organic acids (acetate, succinate, tartrate, citrate, and malate) were determined using an HPLC (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and UV/VIS (210nm ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... and predesigned TaqMan-based qPCR assays for target and housekeeping genes (Table 2) and run on the 7500 Fast Real-Time PCR System and QuantStudio 3 thermocyclers (Applied Biosystems; Waltham, CA, USA). Relative expression values were normalized by the expression of housekeeping genes using the 2-ΔΔCt method ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were pre-concentrated on an Acclaim PepMap 100 pre-column (75 µM x 2 cm, C18, 3 µM, 100 Å; Thermo Fisher Scientific, USA) using the loading pump and buffer A (water ...
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bioRxiv - Bioengineering 2024Quote: ... consisting of a trap column (C18, 75 µm ID x 2 cm, particle size 3 µm pore size 100 Å; Thermo Fisher Scientific, 164946) and an analytical column (75 µm ID x 50 cm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cellular debris was removed by centrifugation at 5,488x g for 40 min at 4°C (Heraeus™ Megafuge™ 40R) and incubated for 2 h with 500 μl HisPurTM Cobalt Resin (89964, ThermoFisher Scientific) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Zebrafish larvae were incubated in 1x E3-medium buffered with 4mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technologies, Carlsbad, CA, USA). Seahorse XF Cell Mito Stress kit (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
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bioRxiv - Systems Biology 2020Quote: ... and 4 μL of this dilution were used to transform One ShotTM ccd B Survival 2 T1R competent cells (Thermo Fisher Scientific, A10460). Transformants were selected with 33 μg/mL chloramphenicol (Merck ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Immunology 2020Quote: ... Lysate equivalent to 2×106 cells per well was run on a NuPAGE 4 to 12% Bis-Tris gel (Thermo Fisher Scientific, USA). Western blots were performed on samples and membranes were probed with anti-PTPN22 (clone D6D1H ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Biochemistry 2021Quote: ... The cells were then fixed by resuspending the cell pellet in 100 uL 2% paraformaldehyde (PFA) - 4% solution diluted in Pbs (Invitrogen, Thermo Fisher, UK) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific, Carlsbad, CA, USA) and 20 μL of a 50% slurry of sepharose Protein A (Rockland Immunochemicals ...
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bioRxiv - Microbiology 2021Quote: ... The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks, Invitrogen) using iBlot 2 Dry Blotting System (Cat ...
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bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was run 2 cm into a 1.5 mm NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (Thermo Fisher Scientific), stained with Coomassie blue and distained with distaining solution (10% acetic acid and 50% methanol) ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and stained overnight at 4 °C with 20 μg/mL of LAMP-2 primary antibody (Thermo Fisher Scientific, MA, USA) in 1 % BSA (in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...