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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were rinsed three times with 1X PBS and coated with ProLong Gold antifade mounting medium with DAPI (4’,6-diamidino-2-phenylindole, 100 ng/ml) (Life Technologies, P-36931) on slides ...
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bioRxiv - Molecular Biology 2022Quote: ... All sections were mounted with 50μl of ProLong Gold Antifade mounting media containing a fluorescent nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, P36931) before imaging ...
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bioRxiv - Cell Biology 2024Quote: Isolated CDX2mCherry cells (n=5) mCherry-negative cells (n=4) and hES cells (n=2 pooled) were centrifuged and 200uL Tri reagent (Thermo Fisher Scientific, USA) was added and incubated at room temperature for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary antibodies were incubated overnight at 4°C before a 2-h incubation with secondary antibodies (1:500, Invitrogen, #A21428 and #A21235) diluted in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and stained overnight at 4 °C with 20 μg/mL of LAMP-2 primary antibody (Thermo Fisher Scientific, MA, USA) in 1 % BSA (in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 45 min in 2% bovine serum albumin (BSA) in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Cell Biology 2024Quote: ... sections were incubated with the secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI) solution (20μg/ml) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: For nuclei and F-actin staining were visualized with 4’,6-diamidino-2-phenylindole (DAPI) (#D523, Dojindo; 1:1000 dilution) and phalloidin Alexa Fluor plus 405-conjugated (#A30104, Invitrogen; 1:400 dilution), phalloidin ATTO 565-conjugated (#94072 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then mechanically chopped at the start of the glial enrichment phase and cultured for 2-4 weeks in base medium supplemented with EGF (Gibco, 20 ng/ml) and leukaemia inhibiting factor (LIF ...
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bioRxiv - Genomics 2024Quote: ... After 2–4 weeks the clones were screened for homologous deletion using the kit Phire Tissue Direct PCR Master Mix (Thermo Fisher Scientific, F170L). Clones with homologous insertion were those showing a mutant band of the expected size and no wild-type band ...
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bioRxiv - Genomics 2024Quote: ... After 2–4 weeks the clones were screened for homologous deletion using the kit Phire Tissue Direct PCR Master Mix (Thermo Fisher Scientific, F170L). Clones with homologous deletion were those showing a mutant band of the expected size and no wild-type band ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were then washed in PBS and the nuclei were counterstained and mounted with 4’,6-diamidino-2-phenylindole (DAPI) SlowFade Gold Antifade Mountant (Thermo Scientific Cat: S36939).48 The stained slides were imaged with 20x objective using the EVOS M7000 imaging system (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... were aspirated using a 21-gauge needle attached to a 5 mL disposable syringe in the presence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered TCM-199 medium (Gibco, Life Technologies, Milan, Italy) and 0.005% (w:v ...
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bioRxiv - Developmental Biology 2024Quote: ... The IVM medium consisted of 25 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid-buffered tissue culture medium 199 (M199; Gibco, Paisley, Scotland, UK) supplemented with 5% calf serum (CS ...
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bioRxiv - Microbiology 2020Quote: The ΔΨ component was measured using tetramethylrhodamine methyl ester (Invitrogen) as described previously14 ...
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bioRxiv - Molecular Biology 2021Quote: ... BODIPY TR methyl ester (1:300, Life Technologies, Darmstadt, Germany) or Vybrant DiO (1:200 ...
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bioRxiv - Physiology 2020Quote: ... Sections were stained with BODIPY TR methyl ester (Thermo Fisher) diluted 1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... Methyl tert-butyl ether (MTBE) was acquired from Acros organics [3787 20010] and acetonitrile was from Honeywell Riedel-de Haën [348512.5L].
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bioRxiv - Neuroscience 2021Quote: To generate construct drg1 [prab-3∷GCaMP6m∷NLS∷unc-54 3’UTR] we performed a 4-way Gateway recombination reaction using LR Clonase II (Invitrogen). We recombined pDEST II with the following entry clones ...
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bioRxiv - Plant Biology 2020Quote: ... peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed before switching to the analytical column (BEH C18 ...
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bioRxiv - Genetics 2020Quote: ... Cells were seeded in 6-well plates and transfected with 4 μg/well vectors expressing CasRx-GFP and gRNAs-mCherry (CasRx:gRNA-1:gRNA-2 = 2:1:1, see Supplementary sequences) using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Control group was only transfected with 2 μg/well vectors containing CasRx-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... The air-dried DNA was dissolved in 19 µL of sterile water and treated with Swa1 in two cycles (2×2 FastDigest units, Thermo Scientific), each cycle of 1 hour at 37°C (total volume 25 µl ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS/2% FBS prior to resuspension in PBS/2% FBS supplemented with 0.5 µm Sytox blue (ThermoFisher Scientific S11348) to assess cell viability ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used (8 μg each): F(ab’)2-goat anti-mouse IgG Alexa 647 & F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher). In WGA-Alexa647 labeling experiments ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was treated with or without (Mock) RNase R (2 U/μg) in the presence of Ribolock(2 U/μg) (ThermoFisher Scientific). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were decolored with 100% ethanol for 2 minutes and incubated for 2 minutes in xylene before mounting with Permount mounting medium (Fisher scientific). Brain slices were digitized using a Zeiss Axio Scan.Z1 slide scanner (Carl Zeiss ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of IL-2 in 2x diluted supernatants was measured using an IL-2 mouse ELISA kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed for 6 min before switching the precolumn in line with the analytical column (BEH C18 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with KIF-encoding plasmid and empty vector pcDNA3.1 (+) (1 µg total DNA) using 2 µl Lipofectamine 3000 Reagent and 2 µl P3000 (Thermo Fisher Scientific). hTERT-RPE1 cells stably expressing SMO-tRFP were transfected a day after seeding and IMCD3 cells expressing mCherry-Arl13b were reverse transfected at the time of seeding ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Biophysics 2022Quote: ... the glass coverslips were mounted onto a Warner chamber and the cells were loaded with 2 µM Fura-2/AM (Molecular Probes) in Ca2+ Imaging Buffer (Ca2+ IB;10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were loaded in loading buffer (2% MeCN, 0.05% trifluoroacetic acid) and bound to an Acclaim Pepmap 100 µm × 2 cm trap (Thermo Fisher Scientific), and washed for 10 min to waste ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...