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Citations for Thermo Fisher :
6301 - 6350 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Roughly 2–5 μL of DNA (~80 ng/mL concentrations) were slowly pipetted into ~50 μL of competent cells (Invitrogen). The cells were left on ice for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... then resuspended at 2 × 107 cells/mL in growth medium supplemented with 5 mM biotin-phenol (biotin tyramide, Acros Organics). After a one-hour incubation ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
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bioRxiv - Developmental Biology 2022Quote: 5-ethynyl-2-deoxyuridine (EdU) pulse labeling was performed with the Click-IT EdU imaging kit (Life Technologies; Cat#:C10340) as described in (Perillo et al. ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in assay medium (RPMI with 10% FBS and 20 IU/mL IL-2), and centrifuged (300xg, 5 mins) (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were washed in PBS with 2% Triton X-100 at 37°C for 5 days and mounted in SlowFade Diamond Antifade Mountant (S36972, Invitrogen). The stained whole mounts were imaged under the two-photon microscope.
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bioRxiv - Cell Biology 2022Quote: ... ATCC® CCL-2) were grown at 37 °C under 5% CO2 in DMEM high glucose glutamax (Gibco, 61965-059) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... and then loaded onto a PepMap C18 trap column (100 μm x 2 cm, 5 μm particle size, Thermo Scientific) followed by separation on an in-house packed column (75 mm x 19 cm ...
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bioRxiv - Neuroscience 2022Quote: ... For in vivo labelling of proliferating SOX10+ cells 5-Ethynyl-2’-deoxyuridine (EdU, 0.3 mg/ml, Thermo Fisher Scientific, E10187) was administered intraperitoneally to pregnant females two hours prior to harvesting of embryos ...
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bioRxiv - Neuroscience 2023Quote: ... To analyze the proliferative capacity of glial cells we injected 5-Ethinyl-2′-deoxyuridine (EdU, 0.05 mg/g, Thermofisher #E10187) intraperitoneally and animals were sacrificed 1hr after injection.
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions (5 × 105 - 2 × 106 total cells) were washed with PBS and resuspended in 1X Annexin binding buffer (Thermofisher) and washed once ...
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bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Microbiology 2023Quote: ... Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Fisher) solution ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, 164199, ThermoFisher) and separated on an in-house packed analytical column i.d ...
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bioRxiv - Immunology 2024Quote: ... were grown as a monolayer at 37°C with 5% CO2 in RPMI 1640 supplemented with 2 mM L-glutamine (31870-025, Thermofisher), 1 mM sodium pyruvate (11360-039 ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Immunology 2023Quote: ... for 2 h at 37 °C and 5% CO2 in the presence of 0.5 mg/ml dextran-rhodamine (Molecular Probes). Untreated Pf sporozoites were used to measure maximum traversal capacity ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: hiPSC-ECTs were then introduced to a Fura-2 loading solution consisting of Krebs–Henseleit buffer supplemented with 5□μM Fura2-AM (Invitrogen) and 1% (vol/vol ...
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bioRxiv - Cancer Biology 2022Quote: The 5-ethynyl-2’-deoxyuridine (EdU) assay was performed following the manufacturer’s instructions (Thermo Fisher Scientific, C10633 Alexa Fluor 488). We used 10μM of Edu for a 2-hour incubation ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... dUTP was added to DNA ends by pelleting stored cells and resuspending in 100 μL of elongation buffer (1X terminal deoxytransferase [TdT] buffer, 2.5 mM CoCl2, 0.3 mM 5-bromo-2’-deoxyuridine [BrdU] [Invitrogen, Waltham, MA]), then adding 5 U of TdT (Thermo-Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Aiptasia or Nematostella polyps were relaxed for 15 min in 0.1M MgCl2 in sea water or Nematostella medium and transferred to sea water or Nematostella medium containing 300 µM EdU (5-ethynyl-2′-deoxyuridine, Invitrogen) 2%DMSO 0.1M MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Cell Biology 2023Quote: ... FACS buffer (5% bovine serum albumin [BSA], 2 mM EDTA, and FluoroBrite Dulbecco’s modified Eagle’s medium [Thermo Fisher Scientific, A1896701]) was used for diluting the antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 µm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Developmental Biology 2023Quote: Timed pregnant females were injected intraperitoneally with 13 μg per g body weight of 5-ethynyl-2’-deoxyuridine (Edu; Invitrogen) and sacrificed after 1 hour or 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and an Acclaim PepMap 100 C18 HPLC nanoViper Trap Column (100Å, 100 μm X 2 cm, 5 μm; ThermoFisher, USA). The tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were injected on a PepMap 100 C18 trap column (100 µm × 2 cm, 5 µM particles, Thermo Fisher Scientific) and separated with an EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific] ...
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was assessed using the 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay (Click-iT EdU Alexa Fluor 488 Imaging Kit, Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... was washed with wash buffer II (150mM NaCl, 50mM Tris pH 7.5, 5% glycerol) on the DynaMag-2 magnet (ThermoFisher Scientific) and added to each lysate and rotated for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...
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bioRxiv - Biochemistry 2023Quote: ... TEV (2): 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of AcTEVTM protease (10 U/µL) (Invitrogen) in SEC buffer ...
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bioRxiv - Genetics 2023Quote: ... 2–5 μg of total RNA was reverse transcribed using a Superscript First-Strand Synthesis system (Invitrogen, Carlsbad, CA, USA). The quantitative RT-PCR was performed using the suh1-4 RT primers (Supplementary Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells at exponential growth phase were incubated with 200 μM of the thymidine analog EdU (5-ethynyl-2′-deoxyuridine - Invitrogen) at 26 °C for 10.2 h (estimated doubling time of L ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated in a 5% CO2 environment at 38.5°C in 2 mL RPMI 1640 media (Gibco, UK) with 10% FBS (Gibco One-Shot FBS ...