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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Leucine Rich Repeats And Immunoglobulin Like Domains Protein 3 LRIG3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... or lacking the CTLH (ΔCTLH) and LISH (ΔLISH) domain were cloned by the Gateway® (Invitrogen) method into pDONR221 entry vector and then recombined into a yeast low-copy number CEN destination vector (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... were used for mouse antibodies and protein G beads were used for rabbit antibodies (Dynabeads, Invitrogen, 10004D). Antibodies used were to CENP-A69 and CENP-C (gift from W ...
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bioRxiv - Pathology 2019Quote: ... Proteins bound to primary antibodies were visualised with peroxidase-conjugated secondary antibodies (Thermo-Fisher Scientific, Brebières, France) and a chemiluminescent detection system (ECL-Plus ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were detected using the following antibodies: anti-MPC1 (BRP44L Polyclonal Antibody, Thermo Fisher Scientific, Roskilde, Denmark), anti-MPC2 (MPC2 (D4I7G ...
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bioRxiv - Biochemistry 2022Quote: ... and polyclonal antibodies were purified from bleeds using protein A purification (Custom Antibody Production, Life Technologies corporation). Western blot analysis was performed using NUPAGE 4-12% gels (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we first extracted total RNA from pools of tissue derived from ten like individuals using TRIzol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... it was necessary to use a fluorescent DNA quantification method with high sensitivity like the Qubit dsDNA HS kit (Invitrogen). Quality assessment ...
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bioRxiv - Biochemistry 2020Quote: ... Further sub-cloning of ZmGl2 and ZmGl2-like sequences for plant expression experiments were performed using LR Clonase II Enzyme Mix (Invitrogen), using pEarleyGate100 vector (Earley et al. ...
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bioRxiv - Neuroscience 2022Quote: ... transfected (psDsRed-2Mito) striatal-like neurons in coverslips were washed with Hanks’ Balanced Salt solution (HBSS) (#14025, Thermo Fisher Scientific). Mitochondrial movement videos were acquired on a Zeiss Cell Observer Spinning Disk System (Carl Zeiss Microscopy) ...
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bioRxiv - Microbiology 2022Quote: ... WTC hiPSCc were passaged as small clumps for maintenance or single cell-like suspension for cardiac differentiation using Versene (Gibco) and mTeSR Plus supplemented with 10 μM Y-27632 (Tocris) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... the cell instructive LEGO-like scaffolds were prepared by loading microgels supplemented with 10 µg/mL of rhVEGF165 (Invitrogen, ThermoFisher) and PDGF-BB (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... the cell instructive LEGO-like scaffolds were prepared by loading microgels supplemented with 10 µg/mL of rhVEGF165 (Invitrogen, ThermoFisher) and PDGF-BB (R&D Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the monolayers containing a mixture of mononucleated CTB-like cells and multinucleated STB cells were partially dissociated using StemPro Accutase (A1110501, Gibco) for 8-12 minutes ...
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bioRxiv - Microbiology 2022Quote: Murine macrophage-like RAW 264.7 cells and human A549 lung epithelial carcinoma cells were cultivated in RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were chosen for this study due to the absence of endogenous ASIC-like currents [53] and were grown using standard procedures in the following medium: Ham’s F-12 Nutrient Mixture (Life Technologies), 10 % fetal bovine serum (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... ATCC CCL-2) cells and 3T3-L1 (murine embryo fibroblast-like cells, ATCC CL-173) were grown in DMEM (Gibco) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... Like samples were combined into a single tube then quantified by high sensitivity DNA Qubit (ThermoFisher scientific, Cat. No. Q32854).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... For both fungi, first we obtained fresh blastospores (i.e., yeast-like single cells) suspended in Grace’s Insect Medium (Gibco, Thermo Fisher Scientific) supplemented with 2.5% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... resuspended in extracellular-like Ca2+-free buffer (120mM NaCl; 5mM KCl; 1mM KH2PO4; 0.2mM MgCl2·6H2O (Fisher Scientific #M35-500); 0.1mM EGTA ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from cryo-pulverized liver tissue and hiPSC-derived hepatocyte-like cells using Trizol (Thermo Fisher Scientific) and 1 ug RNA was reverse-transcribed using SuperScriptTM IV VILOTM Master Mix (#11756050 ...
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bioRxiv - Neuroscience 2019Quote: ... 5µl each from the standards and the rAAV dilutions along with 15µl of mastermix were subjected to qPCR at 95°C 10 min / 95°C 15 sec / 60°C 1 min/ repeat 40×/ melt curve using StepOne machine (Applied Biosystems, USA). Data analyses were performed by StepOne2.3 software and by Microsoft excel.
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bioRxiv - Genetics 2022Quote: We investigated the short tandem repeats (STRs) of the Y-chromosome using the AmpFlSTR® Yfiler® PCR Amplification Kit (Applied Biosystems) and the subsequent data analysis and haplotype determination was carried out with GeneMapper® ID Software v3.2.1 (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... tissue sections were washed 3 times with PBS-T and the following secondary antibodies were used to detect the primary antibodies: Streptavidin-AF555 (Invitrogen), anti-rat IgG2a-AF488 (SouthernBiotech) ...
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bioRxiv - Genomics 2023Quote: ... A western blot was performed on 3 of the monoclonal lines to confirm knockout (primary antibody used: MLH1 Monoclonal Antibody; Invitrogen, Cat No ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies (Supplemental Table 3) followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Immunology 2023Quote: ... D1-3 and D4-5 proteins were fluorescently labelled with Alexa Fluor™ 594 or 488 Microscale Protein Labeling Kits (Invitrogen™, A30008 or A30006) as described [15] ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody-protein-chromatin complex was retrieved by adding 25μl pre-washed Protein G Dynabeads (Thermo Fisher Scientific, 10004D). Immunoprecipitated chromatin DNA was de-crosslinked by 65°C heating overnight using elution buffer (1% SDS ...
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bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antibody proteins were purified from the culture supernatant after 12-14 days using Protein A bead columns (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... CSDE1 protein was then captured by incubation with an affinity purified CSDE1 antibody conjugated to protein A Dynabeads (Invitrogen) for 4 hours at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitation of the protein-antibody conjugate was performed incubating with Dynabeads® Protein A (Thermo Scientific, Carlsbad, CA, USA) 40 minutes at 4ºC in a rotary shaker ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA-protein-antibody complex was captured using protein A/G magnetic beads (Thermo Fisher Scientific, 10002D and 10004D). Magnetic beads were washed with low salt buffer (1X PBS ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were washed with PBS and incubated for 1 hour with secondary antibody [Alexa Fluor-488 goat anti-mouse immunoglobulin G (IgG) (H+L) conjugate or anti-rabbit IgG (H+L) conjugate (Invitrogen)] ...
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bioRxiv - Biochemistry 2020Quote: ... cells were simultaneously incubated with secondary Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (Invitrogen, A-11001, 1:1000) and Streptavidin-Alexa Fluor 647 IgG (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... The secondary immunoglobulins conjugated to horseradish peroxidase were visualized using enhanced chemiluminescence with a commercial kit (ECL Pierce Plus, ThermoFisher) or a homemade solution (100 mM Tris-HCl pH 8.5 ...
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bioRxiv - Immunology 2021Quote: ... Isolation of IgA from a mixture of immunoglobulins was carried out using the agarose-immobilized Jacalin (Thermo Fisher Scientific, USA). For this purpose ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody ZG003 (Thermo Fisher Scientific, Waltham, Massachusetts, US) were incubated at 4 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The antibodies (3 μg/IP) used include anti-GFP mouse monoclonal 3E6 (Thermo Fisher), Anti-GFP rabbit polyclonal A-11120 (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... probed overnight with the following primary antibodies diluted in 3% BSA (Fisher Scientific BP1600): RAI1 (1:1000) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed and allowed to bind with TLR 3 antibody (Invitrogen-PA5-29619) and Alexa 594 conjugated secondary anti-rabbit goat antibody (Invitrogen-A11012) ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-claudin 3 polyclonal antibody (1:100 dilution, PA5-16867, Thermo Fisher Scientific); mouse anti-chondroitin sulfate antibody (1:100 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) (3-8% gradient gels, Invitrogen) and subsequently transferred onto nitrocellulose membranes ...