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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 3 μg of the PRKN plasmid and 1 μg of pCAG-NLS-Bxb1 (9:1 molar ratio) were added in 400 μL of OptiMEM (Thermo Fisher Scientific). Then ...
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bioRxiv - Bioengineering 2023Quote: The lungs of mice were homogenized using a Multi-beads shocker (Yasui Kikai, Osaka, Japan) in 9 volumes of Hanks’ Balanced Salt Solution (Gibco #14025-092). Total RNA was extracted from lung homogenates using an RNeasy Mini kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... capsid plasmid (15 μg per dish) and cargo plasmid (9 μg per dish) were mixed with serum-free DMEM (Thermo Fisher Scientific) and polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Six-μm paraffin-embedded tissue sections were deparaffinized and antigen retrieval was performed by heating the slides for 20 minutes in Tris-EDTA buffer (pH 9) using Lab Vision PT Module (Thermo Fisher Scientific). Sections and coverslips were rinsed 5 minutes in PBS ...
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bioRxiv - Systems Biology 2023Quote: ... then held at 90% B for 9 minutes at a flow rate of 300 nl/min delivered by an Easy1200 nLC system (Thermo Fisher Scientific). All MS1 spectra were collected with orbitrap detection at a 70,000 resolution and a scan range from 300 to 1,500 m/z ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1.5 µg pCig2 Cre T2A H2B-mCherry and 1.5 µg pCA LNL JAM construct was combined with 225 µL of Opti-MEM® with 9 µL of Lipofectamine™ 2000 (Invitrogen). 75 µL of this lipofection formulation was added to 6 wells of Neuro-2a cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated RNA was subjected to cDNA synthesis and further for TaqMan assay specific for mature miR-9 (Applied Biosystems, Cat. No. 4427975).
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bioRxiv - Pathology 2024Quote: Sex-matched 9 to 11-week-old wild-type TGFβ1-Tg+ mice received 0.5 mg/mL doxycycline hyclate (Thermo Scientific Cat No. J60579.22) in their drinking water for 21 days as previously described [35].
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded directly onto a 50 cm long 75 μm inner diameter nano capillary column packed with 1.9 μm C18-AQ resin (Dr. Maisch, Germany) mated to metal emitter in-line with an Orbitrap Fusion Lumos (Thermo Scientific, USA). Easy-Spray NSI source was used ...
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bioRxiv - Genetics 2024Quote: ... We first used semi-automated segmentation to create virtual endocranial imprints in the software Avizo 3D (Version 9; FEI SAS, Thermo Fisher Scientific) following protocols detailed in Neubauer et al.2,88 ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen and X-ray irradiated feeder cells were thawed via dropwise addition into 9 mL DMEM with 15% FBS (15% DMEM) (Invitrogen 12100-046). The cell mixture was spun down at 300 × g for 5 min and the supernatant was decanted ...
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bioRxiv - Immunology 2021Quote: ... The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyl phosphate p-toludine salt (NBT/BCIP chromagen) substrate solution (Thermo Scientific) for 7 min ...
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Synthetic Biology 2022Quote: ... Water extractable halides were measured using an ion chromatograph (Dione ICS-2100, Thermo Scientific). Soil (10 g ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-LAMP reactions were performed in 25 μl volumes supplemented with 1 μM SYTO®-9 double-stranded DNA binding dye (Thermo Fisher S34854) in 96-well plates and incubated at 65 or 60 °C on a realtime qPCR machine (BioRad CFX96) ...
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bioRxiv - Genetics 2019Quote: ... Then 11 μl of cells+SLAT were aliquoted to 9 μl of DNA-SLAT mixtures pre-aliquoted to 96-well polyethylene PCR plates (Thermo Scientific AB-0700). Each gap repair assembly mix had 0.1 μl of each of the three GALR’s PCR product + 8.7μl SLAT ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred onto Immobilon-P PVDF membrane (MilliporeSigma) for 9 min under 1.3A and 25V using the Power Blotter Station (Model: PB0010, Invitrogen by Thermo Fisher Scientific). The membrane was incubated in EveryBlot Blocking Buffer (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... T4 phage were added (3×108 phages ml-1) to the AFM bio-cell together with SYTO 9 and PI (BacLight Bacterial Viability and Counting Kit, Invitrogen, ThermoFisher Scientific, United States) at concentration of 0.06 mM ...
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bioRxiv - Microbiology 2020Quote: ... Real-time qualitative PCR analysis was performed as described previously (9) using an Applied Sciences StepOne Plus Real Time PCR System (Life Technologies, Carlsbad, CA). Primer and probe sequences used for gene expression analysis are listed in Supplementary Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... 9 x 105 cells of each indicated SARS-CoV-2-non-infectable cell type were stained with CellTrace™ Violet (Thermo Fisher # C34557), following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Viral mRNA expression was measured with HIV-1 gag primer probe mix (Supplementary Table 9) and normalized to Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited) (Applied Biosystems). Relative expression was calculated using the ΔΔCt method (47) ...
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bioRxiv - Biochemistry 2020Quote: ... RT-LAMP reactions were performed using WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (M1800) supplemented with 1 μM SYTO®-9 double-stranded DNA binding dye (Thermo Fisher S34854) and incubated on a real-time qPCR machine (BioRad CFX96 ...
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bioRxiv - Neuroscience 2021Quote: ... Fixations and all washings without detergent were done in black glass wares or 9-well PYREX™ Spot Plates (ThermoFisher Scientific, MA, USA) to avoid sticking brains onto plastic.
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bioRxiv - Developmental Biology 2022Quote: ... we found that poly-lysine treating the coverslips was not necessary for immobilization of the worms in most cases as long as the liquid under the agarose pad is minimal.) 7-9% agarose pads (Invitrogen, cat no. 16500500) were gently placed over the top of the worms and excess liquid was wicked away using Whatman paper ...
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bioRxiv - Bioengineering 2019Quote: ... microparticles were fabricated by dissolving PLGA (Lakeshore Biomaterials; cat. #5050 DLG4A) at 20 mg/mL in an organic solution containing 9:1 dichloromethane (DCM; Acros Organics, cat. #364230010) to trifluoroethanol (TFE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reverse transcription PCR was performed with 9 µl of those samples free of DNA with the RT-PCR kit (Applied Biosystems, Karlsruhe, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... then subsequently stained with 1 uL SYTO 9 (for DNA) and 1 uL BacLight Red (for cell wall) Bacterial Stain Ex581/Em644 (Invitrogen, Thermo Fisher, UK). For mounting ...
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bioRxiv - Cell Biology 2021Quote: ... Daily media changes were performed up to day 9 when cells were fixed with 100% ice-cold Methanol (Fisher Scientific, cat # A454-4). Images were acquired on a Nikon Instruments Ti2 inverted fluorescence microscope ...
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bioRxiv - Neuroscience 2021Quote: Whole mouse cortex was dissected from 9-month-old mouse brain and peptides were isolated using the EasyPep™ Mini MS Sample Prep Kit (Thermofisher cat# A40006) following manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Breg adenovirus-transduced brown adipocytes were cultured in serum-free medium with 1× protein transport inhibitor cocktail (10.6 µM Brefeldin A and 2 µM Monensin) (Fisher scientific, #50-930-9), 10 µM oligomycin ...
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bioRxiv - Microbiology 2022Quote: ... The cells were co-transfected with 50 ng of plasmid expressing ISRE reporter (9) and 50 ng of pCMV-Red Firefly Luc Vector (Thermo Fisher Scientific # 16156) using lipofectamine LTX (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 50 uL of water and transferred into 9 mm Clear Glass Screw Thread Vials (1.7 mL Tapered Base,Thermo Scientific™ SKU C40009) with Vial Caps (Thermo Scientific™ ...
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bioRxiv - Microbiology 2023Quote: ... nodule sections were immediately stained with 5µM SYTO 9 and 30µM propidium iodide (PI) using a LIVE/DEAD BacLight Bacterial Viability Kit (Thermo Fisher Scientific Cat. No. L13152) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: mTERT-immortalized mouse CCD cells were cultured as described previously (8, 9) in 1:1 DMEM/F-12 (Gibco GlutaMAX™; Fisher #10565018) with 5% FBS (Gibco One Shot™ ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS; Life Technologies, Grand Island, NY, USA) was performed in each amniotic sac using a 30G needle (BD PrecisionGlide Needle ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... for 30 min or with a mix of SYTO 9 (3.34 µM) and propidium iodide (PI, 20 µM) from the LIVE/DEAD BacLight Bacterial Viability kit (#L7012, Molecular Probes, Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were stained with SYTO 9 and propidium iodide specific for prokaryotes (LIVE/DEAD® BacLight™ Bacterial viability Kit, Molecular Probes L7012) according to the manufacturer’s instructions for 15 minutes at room temperature and no light ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos were mounted on a microscope slide with a Secure-Seal™ Spacer (8 wells, 9 mm diameter, 0.12 mm deep, Thermo Fisher Scientific, S24737), covered with a cover glass ...
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bioRxiv - Microbiology 2024Quote: ... and amplified product was also verified by adding 3 µl of SYBR Green I dye (1:9 dilution, Life technologies Corporation, Eugene, OR) in each sample tube ...
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bioRxiv - Microbiology 2023Quote: ... Novex TBE 6 or 10% gels (Invitrogen) were pre-run in 0.5x TBE running buffer (pH ∼8.4 +/- 0.5) ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and immersed and incubated in the dark in staining solution 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronicacid (X-Gluc, Thermo Scientific), 50mM sodium phosphate buffer ...
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bioRxiv - Plant Biology 2019Quote: To assess GUS expression driven by the maize ubiquitin promoter in transgenic barley transformed with the pBRACT214m-GUS construct we collected different tissues and stained these with 1mg/ml of X-gluc (5-bromo-4-chloro-3-indolyl-B-D-glucuronic acid, Thermo Scientific, USA) in X-gluc buffer (100mM sodium phosphate buffer pH 7.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... 7H11 plates contained 50 μg/mL hygromycin and 50 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (Thermo Scientific). Unless specified ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and 100 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-gal) (Thermo Fisher Scientific). After incubation overnight at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... 5×10-5 M 2-mercaptoethanol (Gibco) and 50µg/mL Gentamicin (Lonza ...