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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were reverse transcribed in the presence of a mixture of oligo dT and random primers (9:1) at 50°C for 50 min using ThermoScript Reverse Transcriptase or SuperScript IV Reverse Transcriptase (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was manually processed with 20 minutes heat-induced epitope retrieval at pH 9 followed by 15 minutes of proteinase K (1μg/ml; Life Technologies) digestion at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Developmental Biology 2021Quote: ... One μL cDNA of each sample was placed in each well and 9 μL of the Master Mix consisting of Power SYBR Green Master Mix (Life technologies), water and primers purchased from MWG ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Molecular Biology 2021Quote: The volume of cDNA was estimated and digested by adding 100 µL of digestion mix (79 µL H2O, 9 µL 10x Fast Digest Buffer (Thermo Scientific), 5 µL ExoI (20 U/µL ...
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bioRxiv - Genomics 2020Quote: ... Samples were run on an Agilent 4200 Tapestation to ensure RNA had a RIN of >9 and quantified via Qubit (Thermo Fisher). PolyA+ purification was performed with 50 μl of NEXTflex polyA+ beads on a minimum of 40 μg of total RNA ...
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bioRxiv - Immunology 2019Quote: Stable cell lines were generated by co-transfection of pcDNA5/FRT/TO and pOG44 in a 9:1 ratio into HeLa FlpIN T-REx cells (kindly provided by the Hentze Lab, EMBL, Heidelberg) using Lipofectamine2000 (ThermoFisher Scientific) and selection with 10 µg/ml blasticidin and 500 µg/ml hygromycin B ...
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bioRxiv - Immunology 2021Quote: ... The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505, Fisher Scientific). The protein content of the viral lysate was quantified using BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: iPSCs (DF19-19-9-11T) purchased from WiCell Technologies were maintained on vitronectin-coated plates in Essential 8 medium (E8, ThermoFisher Scientific), and passaged when the colonies reached ~75-80% confluence ...
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bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
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bioRxiv - Bioengineering 2019Quote: ... The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse) (Invitrogen, Carlsbad, CA), Rabbit anti-Mouse anti -MMP-2 (R & D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... RLuc-HuD 3’UTR was transfected alone or in combination with epB-Bsd-TT-FMR1 or epB-Bsd-TT-eGFP in 5x104 pre-seeded HeLa cells expressing RFP-FUSP525L [9] in a 24-well plate using Lipofectamine 2000 (Life Technologies), following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Cells were transfected with 3.5 µg of each CAS-9-guide and EGFP donor knock-in vector using Lipofectamine 2000 (11668019, Invitrogen, Thermo Fisher Scientific) for 6 hours before replacing media to allow cells to recover ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred onto Immobilon-P PVDF membrane (MilliporeSigma) for 9 min under 1.3A and 25V using the Power Blotter Station (Model: PB0010, Invitrogen by Thermo Fisher Scientific). The membrane was incubated in EveryBlot Blocking Buffer (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... 9 mL blood samples (from the same pool) were poured into 9 mL S-Monovette opened syringes using S1 Pipet Filler (Thermo Scientific) which enabled fatigue-free pipetting ...
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bioRxiv - Plant Biology 2020Quote: Synthetic Arabidopsis RALF1 polypeptide was synthesized by using 9-fluorenylmethyl chloroformate solid-phase chemistry with a peptide synthesizer from Thermo Scientific company and confirmed by MALD-TOF analysis (Applied Biosystems Voyager System 2098 ...
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bioRxiv - Microbiology 2020Quote: ... and groups of 10 midguts were exposed to 2 × 106 parasites in 40uL of PBS in a well of an electron microscopy 9 cavity Pyrex pressed plate (Fisher Scientific). Chambers were transferred to a humidified chamber ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed twice with 0.9% NaCl and 100 μl per well was placed in a 96-well black plate with optical bottom (Thermo Scientific Nunc), in triplicate ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Cancer Biology 2022Quote: Protein extracts were collected in 9 M urea lysis buffer and quantified using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). Protein samples were fractionated with SDS-PAGE using Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Liquid nitrogen was allowed to evaporate (30-60s) before 2mLs of extraction medium consisting of 75% (9:1 v/v) Methanol (ThermoFisher Scientific):Chloroform (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted in 96-well PCR plates in 9 µl of RT/pre-amp mix from the CellsDirect One-Step qRT-PCR Kit (Life Technologies) and were kept at −80°C at least overnight ...
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bioRxiv - Immunology 2020Quote: ... The amplified product was diluted with a ratio of 1 part cDNA to 9 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with a dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit ...
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bioRxiv - Neuroscience 2021Quote: ... The rt-qPCR was performed in triplicates with 9 μl of the diluted cDNA and 2× PowerUp Sybr Green Master Mix (Applied Biosystems) in a 20 μl final volume ...
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bioRxiv - Cancer Biology 2022Quote: ... were combined at a 1:9 ratio and then added to luciferase-stable JeKo-1 cells suspended in Buffer R (Neon Transfection System, Thermo Fisher). The suspension was pulse electroporated at 1550 volts for 30 milliseconds ...
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bioRxiv - Microbiology 2019Quote: ... JE-CVax hyper-immune and non-immune heat inactivated mouse serum samples (starting dilution of 1/9) were serially diluted 3-folds in RPMI 1640 medium (Gibco, ThermoFisher Scientific) supplemented with 4% low IgG serum ...
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bioRxiv - Developmental Biology 2021Quote: ... the cassettes were immersed in 0.9 % NaCl overnight before progressive dehydration and paraffin-embedding using an ExcelsiorTM AS Tissue Processor and HistoStarTM Embedding Workstation (Thermo Scientific). 6 μm-thick transversal sections were made for subsequent immunohistochemistry or in situ hybridization.
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bioRxiv - Neuroscience 2020Quote: ... and 1 mM MEM sodium pyruvate) by pipetting with a fire-polished 9” borosiligate glass Pasteur pipet (Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2021Quote: Responder T-cells were generated by transducing HLA-DP4/WT1 TCR (clone 9) into primary T-cells as shown in Figure 6C and labeled with a tracer dye (CellTrace Violet, Life Technologies). HTLV-1-infected CD4+ T-cells from HLA-DP4+ asymptomatic carriers were as purified as shown in Figure 6C ...
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bioRxiv - Microbiology 2019Quote: Bladder epithelial cells (5637 - ATCC HTB-9) were maintained in RPMI 1640 (Roswell-Park Memorial Institute media –; Thermo Fisher Scientific, MA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: The complete Ubr5 cDNA was synthesised from murine embryonic stem cells total RNA [9] and cloned into a modified pcDNA5/FRT vector (Life Technologies) containing an amino-terminal 2×HA/2×Strep ...
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bioRxiv - Biochemistry 2021Quote: ... to a final concentration of 9 mg.mL−1 that was measured by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham MA USA) with theoretical Mw = 39,390 g.mol−1 and ε280 = 50,037 mol−1.L.cm−1.
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bioRxiv - Microbiology 2020Quote: ... ISP2 and ISP4 (International Streptomyces Project medium 2 and 4; premixed Difco™ ISP2 dehydrated medium (Fisher Scientific, #DF0770-17-9) and premixed Difco™ ISP4 dehydrated medium (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Ten different Bacteroides strains in stationary phase (1mL) were subcultured into 9 mL of liquid sABB (Anaerobe Basal Broth, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... lot#81115172) were thawed to room temperature and slowly diluted with 9 mL pre-warmed RPMI-1640 medium (Gibco, 11875-093) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene fragments encoding multiple (2 to 9) HA-tags were designed in silico and ordered using GeneArt Gene Synthesis service from Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... 5 × 106 cells were plated in 10 cm dishes and co-transfected with 3 μg VSV-G plasmid and 9 μg of the respective construct Lipofectamine LTX (Thermo Fisher) the following day ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Each dried aliquot of the non-polar layer was reconstituted in 50 μl of methanol: toluene (9:1, v/v) (Fisher Scientific) and transferred to 2 mL LC-MS amber vials with 300ul glass inserts ...
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bioRxiv - Plant Biology 2022Quote: Full-length coding regions of DUF581-9/ FLZ3 (At2g44670) and of the DUF581-9C47S variant (Nietzsche et al., 2014) were cloned into pENTR-D/TOPO (Thermo Fisher). For stable overexpression lines ...
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bioRxiv - Physiology 2022Quote: ... Newly eclosed flies from these cultures were collected in the late afternoon and incubated overnight in 9-cm plastic Petri dishes (Fisher Scientific) containing plain sugar agar medium [31] to eliminate the flies that die shortly after eclosion ...
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bioRxiv - Molecular Biology 2023Quote: ... pUC19-tRNA-SerAGA and pUC19-tRNA-LeuCAA were co-transfected into SCC-9 or SCC-15 cells with Lipofectamine 2000 reagent (Invitrogen, 11668019). For ubiquitination assay ...
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bioRxiv - Plant Biology 2022Quote: Oligonucleotide primer sets for real-time (quantitative) PCR were designed using Vector NTI Advance 9 software (Life Technologies, Carlsbad, CA, U.S.A.). They are listed in Supplementary Table TS3 ...
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bioRxiv - Microbiology 2023Quote: ... BSF cells and a T7 polymerase/Cas9 expressing derivative line (derived using plasmid pJ1339)128 were cultured in HMI-9 medium (Life technologies), supplemented with 10% foetal calf serum at 37 °C with 5% CO2 ...
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bioRxiv - Pathology 2023Quote: ... 100% buffered Percoll was made by a 1:9 ratio of 10x PBS to Percoll (Fisher Scientific cat# 45-001-747).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with siRNA oligos at a final concentration of 9 nM by forward lipofection using Lipofectamine 2000© (#11668019, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: SVG-A and 293T cells were infected with JCPyV M1-SVEΔ for 9-11 days and stained first with the LIVE/DEAD® Fixable Aqua Dead Cell Stain Kit (Invitrogen), then cells were split and stained in parallel with one of the following antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM MEM sodium pyruvate) by pipetting with a fire-polished 9” borosilicate glass Pasteur pipet (Fisher Scientific, Waltham, MA).