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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... and subcloned into pENTR/D-TOPO vector (Thermo Fisher Scientific). The resultant vector was used to amplify the coding sequence of H2B-mNeonGreen using a primer pair of MpFGMYBp-AtHTB1-Fw and MpFGMYBt-mNG-Rv ...
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bioRxiv - Microbiology 2023Quote: ... using a pENTR/D-TOPO cloning kit (Thermo Fisher Scientific) as described previously (9) ...
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bioRxiv - Bioengineering 2023Quote: ... Human FLRT2 antibody (#AF2877, Invitrogen R&D Systems, Minneapolis, MN) was added at 20 μg/mL in 100 μl stain buffer per 1x106 cells and incubated for 20 min at room temperature protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... Genes assembled in a pENTR/D-TOPO vector (K240020, Invitrogen) were validated by sequencing ...
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bioRxiv - Genomics 2023Quote: ... and 55 μM β-mercaptoethanol in Knockout D-MEM (Invitrogen) containing 2i (1 μM PD0325901 ...
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bioRxiv - Genomics 2023Quote: ... HCT116-Cas9 cells were washed once with D-PBS (GIBCO) and detached using 0.25% Trypsin (GIBCO) ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into pENTR/D-TOPO vector (Thermo Fisher Scientific). The resulting plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... containing 50μg/ml poly-D-Lysine (Gibco; Thermo Fisher Scientific) coated for 24hr (37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Both SA2 cDNAs were cloned into pENTR/D vector (Invitrogen) and then into an N-terminal YFP-tagged Gateway cloning vector (a kind gift from Endre Kiss-Toth ...
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bioRxiv - Cell Biology 2023Quote: ... using as a template pENTR/D-TOPO (Thermo Fisher Scientific) vector containing cDNA of human TTBK2 ...
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bioRxiv - Immunology 2023Quote: ... cDNAs prepared using oligo d(T)12-16 primers (Invitrogen). The mRNA expressions of the specific gene were quantified by the CFX96 thermal cycler (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... containing 50μg/ml poly-D-Lysine (Gibco; Thermo Fisher Scientific) coated for 24hr (37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... D-Galactose was purchased from Fisher Scientific (part no. BP656). D-Glucose was purchased from Alfa Aesar (part no ...
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bioRxiv - Cancer Biology 2023Quote: ... cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific), and subsequently recombined with pLenti6-V5/DEST using LR clonase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... RAP2.12 and SUB1A coding sequences in pENTR-D/TOPO (Invitrogen) were recombined into pDH51-GW-YFPc (Zhong et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pENTR™/D-TOPO™ (Invitrogen™). The AS2 coding sequence was cloned into the pATML1 containing gateway vector pAR176 (Basta resistance (46) ...
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bioRxiv - Plant Biology 2024Quote: ... cloned into the pENTR-D TOPO vector (Invitrogen, Carlsbad, CA), and then into the pHellsgate 8.0 vector35 using LR Clonase (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... aeruginosa strain PAO1 was cloned into pET200/D-TOPO (Invitrogen), digested with NdeI and BamHI restriction enzymes and then sub-cloned into the standard pET 28(a ...
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bioRxiv - Microbiology 2024Quote: ... with 4.5 g/L D-glucose (Gibco – cat #11995-073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split every 2-3 days through 9 minutes incubation with EDTA 50mM pH=7.4 and 3 minutes of 0.25% trypsin (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... was performed using the Applied Biosystems QuantStudio 3 Real-Time PCR system and analyzed with the QuantStudio 3 Design and Analysis software (v1.5.1,ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: 3′-desthiobiotin labeling was carried following the manufacturer’s’ guidelines of Pierce™ RNA 3′ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Biophysics 2019Quote: ... Procaspase-3 (0.123 mg/mL protein extract) and caspase-3 (1.0 U) were assayed in 96-well microplates (Nunc™ MicroWell™ 96-Well ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pbf1 gene of johansen Standard was amplified with the primer pair psbNRO_F 5’-AGCATTGGGAGGCTCATTAC-3’ and psbNRO_R 5’-GGAAACAGCAACCCTAGTCG-3’ and cloned into to pCRTM2.1-TOPO® (Invitrogen). The vector was linearized with HindIII and in vitro transcription was performed according to the suppliers’ protocol ...
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bioRxiv - Microbiology 2020Quote: ... RdRp_rev 5’-CAAATGTTAAAAACACTATTAGCATA-3’ RdRp_probe 5’-FAM-CAGGTGGAACCTCATCAGGAGATGC-TAMRA-3’) and/or TaqMan gene expression assays (Applied Biosystems) for ACTB (Hs99999903_m1) ...
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bioRxiv - Microbiology 2022Quote: ... 3 µL RT reaction mix consisting of 3 µL of 50 µM random hexamers (Thermo Fisher, cat#48190011), 0.8 µL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Genomics 2021Quote: 3’-desthiobiotin labeling was carried following the manufacturers’ guidelines of Pierce™ RNA 3’ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Immunology 2022Quote: ... and their corresponding FAM-labelled MGB probes (εGLT: 5′-AGGCACCAAATG-3′ and γ1GLT: 5 ′-CTCAGCCAGGACCAAG-3′) (Applied Biosystems) were designed in house ...
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bioRxiv - Neuroscience 2023Quote: ... 5’- CCACCCTCCAGCCAATC-3’ and FAM/ZEN-labeled probe 5’-ACAGGGCAACCATTGGCTCG-3’ with Taqman Gene Expression Master Mix (ThermoFisher).
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bioRxiv - Cancer Biology 2024Quote: ... we isolated mRNA from bone marrow of 3 Ctrl and 3 cKO animals using TRizol (Thermo Scientific, #15596026) and prepared cDNA using 500 ng of total RNA using high capacity cDNA reverse transcriptase (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting topoisomerase 2beta (5’CGAUUAAGUUAUUACGGUUtt 3’, s106; 5’ GAGUGUACACUGAUAUUAAtt 3’, s108; both purchased at Ambion/ThermoFIsher Scientific), TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’ ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting topoisomerase 2beta (5’CGAUUAAGUUAUUACGGUUtt 3’, s106; 5’ GAGUGUACACUGAUAUUAAtt 3’, s108; both purchased at Ambion/ThermoFIsher Scientific), TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’ ...
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...
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bioRxiv - Microbiology 2023Quote: The embB region containing codon 406 was amplified using PCR primers F1 5’-TGATATTCGGCTTCCTGCTC-3’ and R1 5’-TGCACACCCAGTGTGAATG-3’ designed using Primer3 (23) (version 0.4.0) and acquired from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Immunology 2022Quote: ... tissues were minced with a razor blade and subjected to a 30-minute enzymatic digestion at 37 °C in 3 mL of 1:1 DMEM/F12 media (Gibco, Grand Island, NY; 200 mM L-glutamine) with 1X-collagenase/hyaluronidase (10X stock ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Microbiology 2022Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:3000; Thermo Fisher, HYB-076-08-02), rabbit anti- CFP-10 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:1,000; Thermo Fisher, HYB-076-08-02), rabbit anti-CFP-10 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 1×106 HL-60 cells were seeded on cell 75 cm2 culture flask in 6 mL IMDM medium (Thermo Fisher Scientific, Waltham, MA, US) and incubated for 24 h in the presence of compound 1 at 10 nM and 10 μM concentrations ...
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bioRxiv - Microbiology 2020Quote: ... analyzed on a QuantStudio 3 (Applied Biosystems) thermal cycler using the standard cycling conditions ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 3 mM L-glutamine (Gibco), 0.25 mg/mL gentamycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3% O2 in DMEM/F12 (Gibco) with B27 supplement (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... then 3-mm glass beads (Fisher Scientific) and 1 ml fecal lysis buffer (50 mM Tris pH7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... 3 % goat serum (with 0.02 % NaN3) (Invitrogen), 3 % donkey serum (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and run on a QuantStudio 3 (ThermoFisher). All reactions were normalized to the housekeeping gene RpL32 and control flies ...
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bioRxiv - Molecular Biology 2021Quote: ... on a Quant studio 3 (Applied Biosystems) Real-Time PCR Detection machine ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-8% Tris-Acetate gels (Invitrogen EA0375) were used to resolve all proteins ...