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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Protein and ligand concentrations were determined using a NanoDrop One spectrophotometer (Thermo Fisher Scientific). 25 µM of protein and 1 mM of ligand ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The quantity and quality of gDNA was analyzed through Nanodrop Spectrophotometer (Thermo Scientific One). Global DNA methylation was determined calorimetric through MethylFlash Global DNA (5-mc ...
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bioRxiv - Neuroscience 2024Quote: ... Nanosensor concentration was calculated by measuring absorbance at 632 nm (NanoDrop One, Thermo Scientific) with an extinction coefficient of ε = 0.036 (mg/L)-1 cm-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell Signaling Technology) with one Pierce Phosphatase and Protease Inhibitor Mini Tablet (Thermo Fisher) added per ml of cell lysate ...
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bioRxiv - Microbiology 2024Quote: ... The extracts were quantified with a NanoDrop One spectrophotometer (Thermo Scientific, Wilmington, MA, USA) and TapeStation RNA ScreenTape (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of the protein was measured spectrophotometrically using a NanoDrop One (Thermo Scientific) and purity confirmed by SDS-PAGE analysis followed by Coomassie Blue staining.
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bioRxiv - Microbiology 2024Quote: ... The concentration of the RNA extracts was determined using a NanoDrop One device (ThermoFisher).
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bioRxiv - Molecular Biology 2024Quote: ... was run on the Step-One Plus Real- Time PCR system (Applied Biosystems,4376598). Relative gene expression of interleukin 1) ...
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bioRxiv - Biochemistry 2024Quote: All reagents and solvents (including anhydrous ones) were obtained from commercial sources (Fisher Scientific) and used without purification unless otherwise noted ...
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bioRxiv - Physiology 2024Quote: ... RNA concentration and quality were determined using a NanoDrop One spectrophotometer (Thermo-Fisher Scientific). One μg of RNA were reverse transcribed into complementary deoxyribonucleic acid (cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... using CFX Opus 384 Real-Time PCR System with One software (v2.2.2; Applied Biosystems). Hprt and ActA were used as housekeeping genes to normalize the expression ...
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bioRxiv - Genetics 2024Quote: ... DNA quantity and quality was checked on NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher).
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bioRxiv - Molecular Biology 2024Quote: ... The SuperScript III Platinum One-Step qRT-PCR kit (ThermoFisher Scientific, Waltham, MA, USA) was used on a CFX96TM thermal cycler ...
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bioRxiv - Molecular Biology 2024Quote: One-hybrid GFP reporters were ordered as synthetic pieces of DNA from Thermo Fisher Scientific for CAG-GFP ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 µL of Herpes solution and 2.5 mL of 100 mM CaCl2), staining with Annexin V PE (BD Pharmagen™, BD Biosciences, CA, US and 1 mM 7-Aminoactiomycin D (Thermo Fisher Scientific) and analysis by Flow Cytometry using a BD LSRFortessa X20.
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed in PBSE-d and labeled with a goat anti-rabbit APC conjugated secondary antibody (1:500 dilution, ThermoFisher Scientific, cat # 31984) and DAPI (1:5000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... As a control, siGENOME Non-Targeting Pool #1 (Dharmacon, D-001206-13-05) and a second independent predesigned PURA siRNA (Thermo Fisher Scientific, 289567) was used as described for the PURA siRNA pool ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-HuC/D (a pan-neuronal marker to identify the cytoarchitecture and borders of the PVH; 1:500; Life Technologies, Carlsbad, CA), and either a rabbit anti-CRH (PBL#rC68 ...
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bioRxiv - Plant Biology 2020Quote: ... The histone H3.1 gene (At5g65360) and its promoter (1167 bp upstream of the start codon) were cloned into pENTR/D-TOPO (ThermoFisher Scientific, Waltham, MA) and then sub-cloned using Gateway Technology into the plant binary vectors pB7WG (57) ...
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bioRxiv - Microbiology 2020Quote: ... The transformed cells were plated out onto LB agar supplemented with 35 μg/ml chloramphenicol and Isopropyl β-D-1-thiogalactopyranoside/X-gal (Fisher Scientific, USA) and incubated at 37°C for 18 hours and the plasmid was extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: The ORF of OsRZF1 gene without stop codon was amplified (Supplemental Table 1) and cloned to the entry vector with pENTR™/D-TOPO® Cloning Kit (Invitrogen, USA). Then the 2×35s promoter (Marquès-Bueno et al. ...
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bioRxiv - Microbiology 2023Quote: ... washed with D-PBS and further incubated with a monoclonal antibody against CD11c conjugated with PE-Cyanine7 (1:200; Thermofisher 25-0114-82) for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™, Cat. No. 155411, Thermo Scientific) and incubated at 37 °C for 3-5 hours ...
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bioRxiv - Neuroscience 2022Quote: ... the electrode area of all HD-MEAs was treated with poly-D-lysine (PDL, 20 μL, 0.1 mg mL-1; A3890401, Gibco, ThermoFisher Scientific, Waltham, USA) for 1 hour at room temperature and then rinsed three times with sterile water ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis, MN, Catalog no. S13110], 1% bovine serum albumin [Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 4°C) and viable cells were stained using Live/dead fixable yellow stain (1:1000 in 1% FBS/PBS, Molecular Probes, 15 min., on ice). Cells were washed and incubated (15-20 min. ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl of reverse transcription mix (50 mM Tris-HCl, pH 8.3 [Sigma], 75 mM NaCl [Ambion], 1 mM GTP [Thermo Scientific], 3 mM MgCl2 [Ambion] ...
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bioRxiv - Immunology 2021Quote: ... for 3 days and cultured in RPMI 1640 medium supplemented with 10% of FBS and 1% penicillin/streptomycin (all Gibco, Thermofisher). To obtain monocytes ...
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bioRxiv - Developmental Biology 2021Quote: ... and in secondary antibody at 1:500 for 3 hours at room temperature goat (anti-rabbit Alexa Fluor 488, cat#A11008 Invitrogen). Sections were mounted using Vectashield with DAPI.
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bioRxiv - Microbiology 2019Quote: ... 8 x 10^5 HEK-293T/17 cells pre-plated in a 6-well plate were co-transfected with a mass ratio of 1:3 replicon:C-prM-E plasmids using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific). Four hours post-transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4.5 µl of RfxCas13d protein (3 mg/ml) were mixed in 1 µl of nuclease-free water with phenol red (Invitrogen). One to 5 nL of RNPs were injected into one- to two-cell stage Ancistrus sp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an antisense digoxigenin-labeled riboprobe against Nematostella vectensis polycystin 1 was synthesized by using 3’ RACE product as a template (MEGAscript transcription kit; Ambion), and was used at a final concentration of 1 ng/μl ...
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bioRxiv - Biophysics 2022Quote: ... The samples were rinsed with pre-cold 1xPBS 3 times and incubated with the secondary antibody Alexa 594-labeled goat anti-rabbit (1:800, Cat# A11037, Invitrogen) at RT for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... The monolayers were washed 3 times with PBS and stained with Alexa Flour 647 conjugated with goat anti mouse IgG (1:2,000, ThermoFisher, A32728) and Alexa Flour 594 conjugated with donkey anti rabbit IgG (1:1,000 ...
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bioRxiv - Bioengineering 2019Quote: ... rinsed 3 times in HBSS buffer and incubated with Alexa Fluor 488 WGA (1:200, A12379, Life Technologies, Zug, Switzerland) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were incubated with anti-FLAG M2 mouse monoclonal antibody at a dilution of 1:500 for 3 hours at room temperature and secondary antibody conjugated to fluorophore at a dilution of 1:1000 (Invitrogen). 45 images per coverslip were obtained with a Nikon Eclipse TE2000-E pinning disc confocal microscope equipped with an Andor laser system and Borealis beam conditioning unit ...
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Wells were quickly washed once with 0.25% trypsin (by pipetting up and down 3 times) and twice with PBS to remove 3T3 cells before collecting keratinocytes into 1 ml Trizol (Invitrogen). Details of the samples used for polyI:C challenge across the 24 wolf keratinocyte cultures are provided in Supplementary Table S1.
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 times with PBS-T and then 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
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bioRxiv - Microbiology 2021Quote: ... sections were rested at RT for 30 minutes before washing thrice with PBS for 3 minutes each and incubating with 1:200 goat anti-rabbit IgG-AlexaFluor647 (A21245 - Invitrogen) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed 3 times before adding 50 μl of 1:1,000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Invitrogen, # 31430) to the plate and incubated for 1 h at RT ...
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bioRxiv - Bioengineering 2020Quote: ... hPSCs were washed 3 times with PBS and dissociated into single cells by incubation in 1 mL of TrypLE Express (GIBCO) at 37°C for 3 min ...
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All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... sections were incubated for 3 hours with streptavidin-Alexa Fluor 488 (Invitrogen, #S32354; 1:1000 dilution in 10% (v/v) NGS ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed at least 5 times 20 min and transferred to 0.25% PBT with 1:400 TO-PRO-3 (ThermoFisher #T3605) for 2 nights ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 100 ng of yellow fluorescent protein (YFP) cDNAs in each well using 1:3 DNA: Lipofectamine 2000 (11668019, Invitrogen) ratio ...