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Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The following day the sections were washed 3 times for 5 min each in PBS-T and incubated with the corresponding secondary antibodies (all 1:1000, Invitrogen), for 2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed with 1% BSA in PBS (180 x g, 3 minutes) before adding viability dye per manufacturer’s instructions (Invitrogen, #L34989). Antibody binding capacity of cells was assessed using 1 µg/mL DUNP19 and anti-human IgG Simply Cellular bead standards (Bangs Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... for 20 min at RT and incubated with 3% NGS PBS containing secondary antibodies (1:600, Alexa-Fluor 594 anti-mouse IgG, Invitrogen and Alexa-Fluor 488 anti-guinea pig IgG ...
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bioRxiv - Neuroscience 2024Quote: HAP1 WT and SNCA KO cells were seeded for ∼75% confluency and transfected with NHEJ and HR plasmid reporters51 using X-tremeGENE HP DNA Transfection Reagent (1:3 DNA:reagent) in OptiMEM (Gibco #31985062). Images were taken on a Zeiss Axio Observer.D1 outfitted with an Excelitas X-Cite 120 LED GFP light at 24 ...
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bioRxiv - Developmental Biology 2024Quote: ... 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’) and 1 μl of 10 mM dNTP mix (Thermo Fisher). Illumina libraries were prepared by using a modified smart-seq2 protocol [Picelli 2014] using SuperScript IV RT and tagmentation procedure as previously described [Henning 2018] ...
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bioRxiv - Genomics 2024Quote: ... and nuclei were washed once with nuclei suspension buffer (NSB; 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% Superase RNA Inhibitor (Thermo Fisher), 1% 0.2mg/mL Ultrapure BSA (New England Biosciences)) ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed 3 times with 1X PBS and treated with 1 μM C11-Bodipy 581/591 dye (Invitrogen) suspended in 1X PBS for 30 min in the dark at 37oC ...
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bioRxiv - Biochemistry 2023Quote: ... with 60 μL of mobile phase A (0.1% FA in HPLC grade water) at 3 μL/min separated using a 50 cm EASY-Spray column (ES803, ThermoFisher) ramping mobile phase B (0.1% FA in HPLC grade acetonitrile ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µL Lipofectamine 3000 Transfection Reagent (Invitrogen) in Opti-MEM for 4-8 hours at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted in 100 µL of nuclease free water and precipitated at -80 °C for ≥ 3 hours with 1 µL of 15 mg/mL GlycoBlue coprecipitant (ThermoFisher), 10 µL of 3 M sodium acetate pH 5.5 ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Microbiology 2022Quote: ... isolated primary cells were co-cultivated with gamma-irradiated mitotically inactivated NIH3T3 mouse embryonic fibroblasts (MEFs) in a 3:1 mixture of Ham’s F-12 nutrient mix (Life technologies) and DMEM supplemented with 5 % FCS ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected using Alexa Fluor 488 labeled goat anti-rabbit IgG antibodies diluted 1:1000 in PBS containing 3% BSA (Invitrogen). Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then washed three times for 3 min in PBST and incubated in an anti-rabbit AF488 (1:2000, Invitrogen) and DAPI (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... 1µg of DNA and 3µl of FuGENE® (1:3 ratio) were combined with 100µl of Opti-MEM™ (Gibco), vortexed and incubated at room temperature for 30 minutes before adding to the cells ...
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bioRxiv - Cell Biology 2023Quote: ... the iMACs were washed 3 x 5 min with PBS and incubated for 1h with 1:1000 goat anti-mouse IgG-AF488 (A11001, Invitrogen). Afterwards the cells were again washed 3 x 5 min with PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twelve hours after transfection ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation, Oregon, USA) followed by washing with 1x DPBS (nuclei data not shown) ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated for 1 hr at room temp in the following secondary antibodies at 1:500 dilution in PBS + 3% BSA: Alexa Fluor 488 Goat Anti-mouse IgG2a (Invitrogen) (binds the CD24 primary antibody) ...
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bioRxiv - Immunology 2023Quote: ... and 100 µl of the homogenate were added to 1:3 v/v of TRIzol™ LS (Thermo Fisher Scientific) and incubated for 10 min at room temperature to inactivate the virus ...
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bioRxiv - Microbiology 2023Quote: ... mouse embryonic fibroblasts 3T3-J2 cells (kind donation from Howard Green, Harvard Medical School, Boston52) with keratinocyte culture medium cFAD (3:1 DMEM (Gibco) to Ham F-12 Nut Mix (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twelve hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HA-Gα-LgBiT constructs and SmB-cmyc-PDZ-RhoGEF were co-transfected using a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). After 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were denatured by adding 3× SDS sample buffer (containing 188 mM Tris-HCl, pH 6.8; 3% SDS; 30% glycerol; 0.01% bromophenol blue; and 1× Bond-Breaker TCEP solution from Thermo Fisher Scientific) and heating at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twenty-four hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... at 70 °C for 7 min then precipitated in 75% ethanol with 10% volume of sodium acetate (3 M, pH 5.5) and GlycoBlue (1 μL, Invitrogen AM9515). The RNA fragments were dissolved in nuclease-free H2O at a concentration of 100 ng/μL ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed 3 times with 5% milk TBST and incubated with HRP-conjugated secondary antibodies (1:5000, 32230/32260; Invitrogen). Data were visualized using chemiluminescence detection on ChemiDoc Touch (Bio-Rad Laboratories).
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... and chromosome III probes (3 BACS covering approximately 1% of the chromosome) were labeled with AlexaFluor 488 (Molecular Probes, F32947).
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bioRxiv - Immunology 2023Quote: ... 1×106 primary human keratinocytes were seeded into the inserts and kept submerged in a 3:1 DMEM (Hyclone, #SH30243.01) and F12 (Gibco, #31765035) mixture with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies (Supplemental Table 3) followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with the indicated plasmids routinely using 1 μg of DNA mixed with 3 μl of Lipofectamine 2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Developmental Biology 2023Quote: ... Stained samples were washed (3 x 5 minutes) and incubated in DAPI solution (Invitrogen, D3571; diluted 1:1000 in DPBS) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissolved tissue was subsequently washed 3 times with 1% FBS solution in 1x DPBS (Dulbecco’s phosphate-buffered saline, ThermoFisher Scientific) and passed through 75 μm and 35 μm cell strainers to remove debris.
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bioRxiv - Biochemistry 2024Quote: ... cartilage shavings were submerged in 3:1 methanol:water and homogenized using a tissue homogenizer (SPEX Sample Prep 1200 GenoLyte, Fisher Scientific). Homogenization included 15 cycles of homogenizing for 20 seconds and resting for 2 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... with 60 μL of mobile phase A (0.1% FA in HPLC grade water) at 3 μL/min separated using a 50 cm EASY-Spray column (ES803, ThermoFisher) ramping mobile phase B (0.1% FA in HPLC grade acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-1 HexaPro S) at 3 µg/mL were placed into 384-well Nunc Maxisorp plates (ThermoFisher, 464718) in 1x TBS and incubated for 1 hour at 37°C followed by slap drying and blocking with 80 µL of Casein for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... separation was performed using a NuPAGE Novex 3%–15% Tris-Acetate Mini Gel with 1× NuPAGE Tris-Acetate SDS running buffer (Invitrogen). Following an overnight transfer of proteins onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... membrane for 3 h at 60 V and detected with anti-GFP primary antibody (1:2000 mouse monoclonal GF28R, Invitrogen), horseradish peroxidase-conjugated rabbit anti-mouse secondary antibody (1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were also probed with a mouse monoclonal antibody targeting glyceraldehyde-3-phosphate dehydrogenase (mAb-α-GAPDH; 1:8000; ThermoFisher).
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bioRxiv - Genetics 2024Quote: ... Cells were seeded with 300 000 cells/well in 6-well plates and transfected 24 hours later with 1 µg of each minigene construct mixed with 3 µL of Lipofectamine 2000 Reagent (Invitrogen) per well ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a second incubation for 3 min in PBS with LysoTracker Red (L7528, Invitrogen; 1:2,000 in 1x PBS) and HOECHST (H1399 ...
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bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3× 5min with 1×PBST and incubated with mouse or rabbit secondary antibodies conjugated with horseradish peroxidase (Invitrogen) for 1 h at room temperature followed 3 × 5 min wash by 1× PBST ...
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bioRxiv - Plant Biology 2020Quote: ... The amplicon was cloned into pENTR/D TOPO vector (Invitrogen). The entry vector was then shuttled into the binary vector pMDC99 [54] using Gateway technology (Invitrogen) ...