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Citations for Thermo Fisher :
6051 - 6100 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: The human dynein motor domain locked in α-registry was expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described (21) ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM L-glutamine at 37°C and in 5% CO2. Human RPE-1 cells (a gift from Dr. Hongtao Yu) were cultured in DMEM: F-12 medium (Invitrogen) supplemented with 10% FBS and 10mM L-glutamine at 37°C and in 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: Human cervical carcinoma HeLa cells (CCL-2, ATCC) were cultured in DMEM/F-12 media (Gibco, Thermo Scientific, MA, USA) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin antibiotics at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Biochemistry 2024Quote: Human cervical carcinoma HeLa cells (CCL-2, ATCC) were cultured in DMEM/F-12 media (Gibco, Thermo Scientific, MA, USA) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin antibiotics at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Microbiology 2024Quote: Immortalized human HaCaT keratinocytes [46] and murine K38 keratinocytes [59] were cultured in high glucose DMEM media (GIBCO 11965-092) supplied with penicillin and streptomycin (GIBCO 15140-122 ...
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes from PBMC buffy coats were isolated with anti-human CD14 magnetic beads (Miltenyi) and cultured in RPMI containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 50 U/ml penicillin ...
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bioRxiv - Immunology 2024Quote: ... 1×106 cells were stained with the panel of antibodies in 100 µl of staining buffer containing 0.5 % human serum albumin (HSA, Albunorm, Octapharma) and 2 mM EDTA (ThermoFisher Scientific) in PBS (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: Primary human skeletal muscle cells were seeded and differentiated on 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) coated with laminin (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... chronic infected patient and HD groups by Luminex using the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (#EPX450-12171-901 ThermoFisher) according supplier’s recommendations ...
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bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Cell Biology 2024Quote: ... in this study were generated from deidentified commercially available human tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (Thermo Fisher) as described before24 ...
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bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
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bioRxiv - Microbiology 2024Quote: ... with a plasmid encoding for a human furin ectodomain (residues 1–794) into 40 mLs of FreeStyle 293-F cells (ThermoFisher). Cells were transfected at a density of 1 million cells/mL using 25 kDa linear polyethylenimine (PEI) ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
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bioRxiv - Cell Biology 2024Quote: ... the human embryonic kidney cell line HEK293T (purchased from ATCC) and the human hepatocellular carcinoma HepG2 (purchased from ATCC) were maintained in DMEM medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... The iPSCs were resuspended in human embryonic stem media with 10 ng/mL of bFGF (beta fibroblast growth factor, Invitrogen) (HES-10 ...
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bioRxiv - Microbiology 2024Quote: The HT29-MTX human colorectal adenocarcinoma cell line 37 was grown in Dulbecco’s modified Eagle medium (DMEM, supplied by Thermo Fisher) supplemented with 10% heat-inactivated (HI ...
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA-TruB1 was generated by inserting the ORF of human TruB1 with a Flag sequence into pcDNA.3.1(+) (Thermo Fisher Scientific) at the Nhe1 and EcoR1 sites ...
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bioRxiv - Cell Biology 2019Quote: ... and SqCC/Y1 cells and primary keratinocytes were routinely maintained in EpiLife medium (with 60 mM calcium concentration) (MEPI500CA) supplemented with Human Keratinocyte Growth Supplement (HKGS; ThermoFisher Scientific). For the experiments ...
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bioRxiv - Cell Biology 2020Quote: ... pseudokinase domain of mouse and human MLKL were expressed in Sf21 insect cells using the Bac-to-Bac system (ThermoFisher Scientific) and purified using established procedures28 ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNAs (siRNAs) specifically targeting human CD44 (siRNA IDs s2681 and s2682) were obtained from Life Technologies (Carlsbad, CA, USA). DPSCs (1×105 per well ...
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bioRxiv - Cell Biology 2019Quote: ... cells were subsequently pulsed at 370C for different time points with 25µg/ml Alexa flour-488 or- 568-conjugated human transferrin (Invitrogen, ThermoFisher Scientific) reconstituted in starvation medium and clarified by centrifugation at 15,000 × gmax at 40C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher). Libraries were then purified via solid phase reversible immobilization (SPRI ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Relative mRNA levels of a panel of inflammation-associated genes were initially screened using a Human Immune Taqman PCR array (4418718, ThermoFisher Scientific) and Taqman Fast Advanced Master Mix ...
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bioRxiv - Neuroscience 2020Quote: Human iPSCs were maintained as feeder-free cells in Essential 8 medium on vitronectin-coated plates (all from Gibco, Carlsbad, CA) (69) ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA samples were used for global gene expression profiling on human Clariom S Assay microarrays (Thermo Fisher Scientific, Wilmington, DE, USA), which include 24351 genes ...
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bioRxiv - Immunology 2022Quote: ... Blots were washed 3x with PBS-T and incubated for 1 h at room temperature with appropriate secondary antibodies: mouse IgGk-BP-HRP (cat# sc-516102, SantaCruz) or goat anti-human-HRP (cat# 31410, Thermo Scientific), diluted 1:10,000 in 5% blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pulsed with either 100µg/ml transferrin from human serum conjugated with Alexa Fluor™ 647(#T23366; Thermo Fisher Scientific) or vehicle (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1 ...
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bioRxiv - Neuroscience 2021Quote: ... of human iPSC-induced neurons were plated on completely dry PDL- (100 µg/ml) and laminin-(10 µg/ml; Thermo Fisher) coated 96-well or 6-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Bioengineering 2022Quote: The human monocyte cell line THP-1 was purchased from ATCC (TIB-202) and routinely cultured in RPMI 1640 medium (Life Technologies) containing 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... were incubated with 2.105 primary human mast cells (cell line established from a different donor) with or without anti-CD3/CD28-coated beads (Dynabeads™, Gibco) at a T cell:bead ratio of 10:1 in RPMI 10% FCS supplemented with 20 ng.mL−1 SCF ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-human CD69 together with Live/Dear-near IR (1:1000 in PBS; LD-NIR; ThermoFisher Scientific; Cat no L10119) for exclusion of dead cells ...
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bioRxiv - Immunology 2021Quote: ... The remaining cells were enriched for CD8+ cells with Dynabeads™ Untouched™ Human CD8 T Cells Kit (Thermo Fisher #11348D) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: CNVs were detected using the Affymetrix Genome-Wide Human SNP Array 6.0 (Cat No. 901182, ThermoFisher Scientific Inc., Waltham, MA, USA) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2019Quote: ... Human IgG and IgM was quantified in blood serum (1:1000 dilution) and culture supernatants (DILUTION) using the Human IgG/IgM total ELISA Ready-SET-Go! Kits (Invitrogen/eBioscience). All ELISAs were performed as per manufacturer’s protocols ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... Cells were re-suspended at 100µL/well with 1:1000 diluent with anti-human IgG-Alexa 488 (#A28175, ThermoFisher, Waltham, MA). Plates were incubated for 1 hour at 4°C ...