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Citations for Thermo Fisher :
5901 - 5950 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...
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bioRxiv - Microbiology 2022Quote: ... PBLs as well as human Jurkat T lymphocytes (human T-cell leukemia cell line) (ATCC-CRL-2899TM) were cultured in RPMI 1640 plus Glutamax (GIBCO) supplemented with 10% fetal bovin serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 2×105 cells were transfected with 20 pmol of ON- TARGETplus Human HMG20A (10363) siRNA-SMART pool (Dharmacon) using Oligofectamine™ according to the manufacturer’s instructions (Invitrogen). Cells were cultured for 3 days before being harvested for follow-up experiments.
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bioRxiv - Neuroscience 2022Quote: ... human BDNF-biotin (Alomone labs; B-250-B) was conjugated to Quantum dots-605 (QD-605) (Thermo Fisher Scientific; Q10103MP). Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... or Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (1:30 bead:T-cell ratio; Gibco) or (2 ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... the RAD54L2 cDNA was assembled from a partial cDNA clone encoding Met109 to Lys1467 was from the human ORFeome collection (Lamesch et al, 2007) and a synthetic DNA fragment encoding Met1 to Glu108 (Invitrogen). RAD54L2K311R was constructed by QuikChange site-directed mutagenesis with primers (5’-AGATCACTTGCAAAGTTCTCCCCAGACCCATGCTG-3’ and 5’-CAGCATGGGTCTGGGGAGAACTTTGCAAGTGATCT-3’) ...
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bioRxiv - Cell Biology 2024Quote: ... that were derived from healthy human donor cells (isWT1.14, kindly provided by the UMG Stem Cell Unit) were cultured in StemFlex medium (Gibco A3349401) and differentiated to atrial cardiomyocytes according to an established protocol (Kleinsorge & Cyganek ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: Organoid culture supernatants and lysates were analyzed for secreted proteins using single- and multiplexed Human ProcartaPlex™ Panel (Thermofisher Scientific), following previously documented procedures.37,38 The plates were read using the Luminex MAGPIX xMAP instrument and xPONENT® software ...
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bioRxiv - Microbiology 2024Quote: ... Huh7 human hepatoma and 293T cells (Riken, Japan) were grown and maintained in Dulbecco’s modified Eagle medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM GlutaMAXTM (4.5 g/L D-glucose) (Life Technologies #10-566-024) supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The Pro-Pol fusion HA-tagged (ProWTPolWT and ProMUTPolWT) plasmids were generated using an in-house human PGK promoter-driven lentiviral vector via Gateway cloning (Invitrogen). Plasmids expressing HuSaV (GI.2 ...
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bioRxiv - Cell Biology 2024Quote: ... The process entailed cloning the various human ACTL7B gene variants into the pENTR TOPO vectors using the pENTR d-Topo cloning kit (Invitrogen), following the manufacturer’s guidelines ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: African green monkey kidney cells (Vero) and Human Embryonic Kidney 293T (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish Kcnc3a channel was labeled using a polyclonal antibody originally generated against the human Kcnc3 protein (ThermoFisher Scientific PA5-53714) at a concentration of 2.5 μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated human Anti-CD9 antibody (MEM-61; catalog no. MA1-19485) and biotinylated human anti-CD81 antibody (M38; catalog no. ab239238) were purchased from Thermofisher Scientific (Stockholm ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... the mucosal layer was removed from an approximately 1 cm3 fresh section of human colonic tissue and washed twice with 1X Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher, 14040133). The mucosa was transferred to a 5-ml conical tube containing 0.5 ml crypt isolation buffer (2 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... whole bone marrow cells from B6 mice or human cord blood mononuclear cells were first prepared in I2 medium (IMDM (Gibco) plus 2% FBS and penicillin/streptomycin ...
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bioRxiv - Genomics 2024Quote: ... human chronic myelogenous leukemia cancer cell line K562 and human embryonic epithelial kidney cell line HEK293T were cultivated in Dulbecco’s Modified Eagle Medium with high glucose (#11965084, Life Technologies) supplemented with 10% fetal bovine serum Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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bioRxiv - Immunology 2024Quote: ... Bound mAb was detected using goat anti-human (H+L)-HRP (1:1000 dilution) (Thermo Fisher Scientific, Waltham, MA, USA). In the case of His-tag detection ...
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bioRxiv - Bioengineering 2024Quote: Human BJ fibroblasts (ATCC CRL-2522) were cultured on standard cultureware in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco 11965-092) with 10% Fetal Bovine Serum (FBS ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... Acquired spectral data files were searched against the human component of UniProtKB/SwissProt database using SEQUEST search engine in the Proteome Discoverer 1.4 (Thermo Scientific). The following parameters were applied ...
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bioRxiv - Cancer Biology 2023Quote: ... at different timepoints as the percentage of hCD20pos cells (anti-human CD20 FITC, clone 2H7) compared to murine CD45pos cells (anti-mouse CD45 PE, clone 30F11, ThermoFisher). When indicated ...
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bioRxiv - Microbiology 2023Quote: ... a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (1:500 dilution; #43700, Invitrogen) and a peroxidase-conjugated goat anti-mouse secondary antibody (1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Human intestinal epithelial cells (HIEC-6) were cultured in Opti-MEM™ I Reduced Serum Medium (Thermo Scientific, MA, USA). Cell viability was measured using MTT assays (ref) ...
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bioRxiv - Bioengineering 2023Quote: ... A 10% (w/v) solution of biomaterial was prepared by manually mixing the pulverized biomaterial in Human endothelial serum free media (HESFM; Gibco) supplemented with fetal bovine serum (2% v/v ...
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bioRxiv - Developmental Biology 2023Quote: A small piece of primary human or mouse tissue was resected and embedded in Fisher Healthcare Tissue-Plus Optimum Cutting Temperature (OCT, ThermoFisher). This was frozen at -80 0C ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Cancer Biology 2023Quote: The 387 complex genetics sarcomas were analyzed on Human Genome U133 Plus 2.0 array (900466, Affymetrix, Santa Clara, CA, USA), according to the manufacturer’s procedures ...
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bioRxiv - Microbiology 2022Quote: ... PBMCs were isolated by density gradient using Lymphoprep (Axis-Shield) and CD4+ T cells purified by negative selection using the Dynabeads Untouched Human CD4+ T Cell Isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: 1000-3000 IEQ human islets pellets were resuspended in 200 µL PBS and treated with 20 µL 10 mg/mL Rnase A (Invitrogen) and 20 µL Protein Kinase K (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... cells were harvested and stained for cell surface CD4 expression using anti-human CD4 APC (RPA-T4, eBioscience, ThermoFisher Scientific) and analysed by flow cytometry on a BD FACSCanto II system (BD Biosciences ...
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bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: RT-qPCR with TaqMan primer/probes was used to determine relative expression levels of miR-146a compared to U18 (human) or sno251 (mouse) endogenous control genes (ThermoFisher, assay IDs ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Cell Biology 2022Quote: ... and maintained in Ultiβ1 cell culture medium (Human Cell Design) containing 100 U/mL penicillin and 100 μg/mL streptomycin (Life Technologies). Based on the experimental setup ...